Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q608N7 (SYL_METCA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MCA1453
OrganismMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]
Taxonomic identifier243233 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus

Protein attributes

Sequence length887 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 887887Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152042

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif624 – 6285"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6271ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q608N7 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 72535449F9AD8F20

FASTA88799,746
        10         20         30         40         50         60 
MEETYNPKTV EETAQRIWEE SGVFRAREDS GREKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VIARYQRMRG KNVLQPMGWD AFGLPAENAA MQNGVTPAAW TDANADYMRK QLKSLGLAVD 

       130        140        150        160        170        180 
WDREIATCKP EYYRWEQWLF TRLFEKGLIY KKTAPVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVEKRDIPM YFMRITAYAE ELLAELDDLP GWPEQVKTMQ RNWIGKSHGC EVHFPYEDGS 

       250        260        270        280        290        300 
DVLKVYTTRP DTLMGATYVA VAAEHPLALE AARDNPELAA FIEECRHGGT AEADLATMEK 

       310        320        330        340        350        360 
KGMATGRFVV HPLNGEKLPV WIANYVLWGY GEGAVMAVPA HDERDFEFAN KYGLPIKTVI 

       370        380        390        400        410        420 
VSTVGAYETV DTKGGWIDAY AEHGRCTNSG KYDGLAFQQA FDAIAADLEA KGLGQKRTQF 

       430        440        450        460        470        480 
RLRDWGISRQ RYWGCPIPII HCLSCGDVPV PAEQLPVVLP EDVTIDVGSP LKKMPEWYST 

       490        500        510        520        530        540 
TCPKCGGAAE RETDTMDTFV ESSWYYARYT CPDNTEAMLD RRADYWLPVD QYVGGIEHAI 

       550        560        570        580        590        600 
LHLLYARFFH KLMRDAGLVH CDEPFTNLLT QGMVVAPTFY REEGGKKHYF GPTEVELSTD 

       610        620        630        640        650        660 
ERGRPLGATL KSDGAPVTVG HIEKMSKSKN NGVDPEALID RYGADTVRLF TMFAAPPDQS 

       670        680        690        700        710        720 
LEWSDSGVEG AFRFLKRLWT RAAFSVNTAF SQYGRDELRV TDWALVPLAP NHKEARRMIH 

       730        740        750        760        770        780 
ATLKQANFDF VRHQFNTVVS AAMKILNTLA DDKGFWNCDH LPEGDEKDAF RRSAAVVAFE 

       790        800        810        820        830        840 
GYSLLTRLLY PIAPHICDAL WRQLMPGTDI LDAGWPEVDE AALAQDEIEL VVQVNGKLRG 

       850        860        870        880 
KITVPADASR ETVEQIALAD AQVLRFVEGK PPKRVIVVPG KLVNVVA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017282 Genomic DNA. Translation: AAU92547.1.
RefSeqYP_113910.1. NC_002977.6.

3D structure databases

ProteinModelPortalQ608N7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243233.MCA1453.

Proteomic databases

PRIDEQ608N7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU92547; AAU92547; MCA1453.
GeneID3103369.
KEGGmca:MCA1453.
PATRIC22606742. VBIMetCap22254_1483.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METCA
AccessionPrimary (citable) accession number: Q608N7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries