Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:MCA1453
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
ProteomesiUP000006821 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 887887Leucine--tRNA ligasePRO_0000152042Add
BLAST

Proteomic databases

PRIDEiQ608N7.

Interactioni

Protein-protein interaction databases

STRINGi243233.MCA1453.

Structurei

3D structure databases

ProteinModelPortaliQ608N7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi624 – 6285"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q608N7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEETYNPKTV EETAQRIWEE SGVFRAREDS GREKFYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VIARYQRMRG KNVLQPMGWD AFGLPAENAA MQNGVTPAAW
110 120 130 140 150
TDANADYMRK QLKSLGLAVD WDREIATCKP EYYRWEQWLF TRLFEKGLIY
160 170 180 190 200
KKTAPVNWDP VDQTVLANEQ VIDGRGWRSG ALVEKRDIPM YFMRITAYAE
210 220 230 240 250
ELLAELDDLP GWPEQVKTMQ RNWIGKSHGC EVHFPYEDGS DVLKVYTTRP
260 270 280 290 300
DTLMGATYVA VAAEHPLALE AARDNPELAA FIEECRHGGT AEADLATMEK
310 320 330 340 350
KGMATGRFVV HPLNGEKLPV WIANYVLWGY GEGAVMAVPA HDERDFEFAN
360 370 380 390 400
KYGLPIKTVI VSTVGAYETV DTKGGWIDAY AEHGRCTNSG KYDGLAFQQA
410 420 430 440 450
FDAIAADLEA KGLGQKRTQF RLRDWGISRQ RYWGCPIPII HCLSCGDVPV
460 470 480 490 500
PAEQLPVVLP EDVTIDVGSP LKKMPEWYST TCPKCGGAAE RETDTMDTFV
510 520 530 540 550
ESSWYYARYT CPDNTEAMLD RRADYWLPVD QYVGGIEHAI LHLLYARFFH
560 570 580 590 600
KLMRDAGLVH CDEPFTNLLT QGMVVAPTFY REEGGKKHYF GPTEVELSTD
610 620 630 640 650
ERGRPLGATL KSDGAPVTVG HIEKMSKSKN NGVDPEALID RYGADTVRLF
660 670 680 690 700
TMFAAPPDQS LEWSDSGVEG AFRFLKRLWT RAAFSVNTAF SQYGRDELRV
710 720 730 740 750
TDWALVPLAP NHKEARRMIH ATLKQANFDF VRHQFNTVVS AAMKILNTLA
760 770 780 790 800
DDKGFWNCDH LPEGDEKDAF RRSAAVVAFE GYSLLTRLLY PIAPHICDAL
810 820 830 840 850
WRQLMPGTDI LDAGWPEVDE AALAQDEIEL VVQVNGKLRG KITVPADASR
860 870 880
ETVEQIALAD AQVLRFVEGK PPKRVIVVPG KLVNVVA
Length:887
Mass (Da):99,746
Last modified:November 23, 2004 - v1
Checksum:i72535449F9AD8F20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA. Translation: AAU92547.1.
RefSeqiWP_010960729.1. NC_002977.6.
YP_113910.1. NC_002977.6.

Genome annotation databases

EnsemblBacteriaiAAU92547; AAU92547; MCA1453.
KEGGimca:MCA1453.
PATRICi22606742. VBIMetCap22254_1483.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA. Translation: AAU92547.1.
RefSeqiWP_010960729.1. NC_002977.6.
YP_113910.1. NC_002977.6.

3D structure databases

ProteinModelPortaliQ608N7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243233.MCA1453.

Proteomic databases

PRIDEiQ608N7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU92547; AAU92547; MCA1453.
KEGGimca:MCA1453.
PATRICi22606742. VBIMetCap22254_1483.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33009 / NCIMB 11132 / Bath.

Entry informationi

Entry nameiSYL_METCA
AccessioniPrimary (citable) accession number: Q608N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 23, 2004
Last modified: April 1, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.