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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathway:iglycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 4091NucleophileUniRule annotation
Active sitei462 – 4621Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:MCA1475
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
ProteomesiUP000006821 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7407401,4-alpha-glucan branching enzyme GlgBPRO_0000188715Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243233.MCA1475.

Structurei

3D structure databases

ProteinModelPortaliQ608L5.
SMRiQ608L5. Positions 118-726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiRRYHHDR.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q608L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDSSTLAEL SPDFQRIVEA RHHDPFAVLG RHRRDNRDLI RAFLPQAEEV
60 70 80 90 100
RVGSAGRVME RIAGTAIFEC EVEAETTDLH YRLYWTDQTG QTHSFIDPYT
110 120 130 140 150
FPPRLSDFDL YLFGEGRHWN IYRVLGAHPH SVDGIDGILF ATWAPNAERI
160 170 180 190 200
SVVGEFNGWD GRRHPMRVRG ASGVWELFIP ELQPGLLYKF EIRNRAHGTI
210 220 230 240 250
HLKSDPYGRQ FELRPNTASI ITRESGYAWN DADWLAQRKD WPWLHRPLSV
260 270 280 290 300
YEMHAGSWKR DLEGGYLNYR DLAHELVDYV KSAGFSHIEL MPVTEHPLDA
310 320 330 340 350
SWGYQTTGYF APTSRFGTPD DFRYFVDHCH RNGIGVILDW VPAHFPKDAH
360 370 380 390 400
GLARFDGTAL YEHEDPRLGE HRDWGTLIYN YGRNEVKNFL LGSALFWLEE
410 420 430 440 450
FHLDGLRVDA VASMLYLDYS RQPGDWIPNK YGGNENLEAI AFLRDLNTVV
460 470 480 490 500
HQQFPGVLVI AEESTAWPQV TRPTWTGGLG FSMKWNMGWM HDILVYMGKD
510 520 530 540 550
PVHRHYHHDQ LTFGLLYAFT ENFVLPFSHD EVVHGKGSML AKMPGDEWRR
560 570 580 590 600
FANLRVLYTM MFTYPGKKLL FMGCEFAQTG EWNHTTALDW PLLESNLHKG
610 620 630 640 650
VLHLVSDLNR LYQSTSALYA YDFESQGFEW IDSHDAAQSV ISYVRRDDDS
660 670 680 690 700
HVVVVLNFTP VPRHNYRIGV PEPVGYREVF NSDAECYGGA NLGNWEIKTE
710 720 730 740
SVEWMGRAQS VVLTLPPLAG IVLAPVAPSA TPDCGTPGDE
Length:740
Mass (Da):84,735
Last modified:November 23, 2004 - v1
Checksum:i872F7568D25B2D0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA. Translation: AAU92509.1.
RefSeqiWP_010960751.1. NC_002977.6.

Genome annotation databases

EnsemblBacteriaiAAU92509; AAU92509; MCA1475.
KEGGimca:MCA1475.
PATRICi22606786. VBIMetCap22254_1505.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA. Translation: AAU92509.1.
RefSeqiWP_010960751.1. NC_002977.6.

3D structure databases

ProteinModelPortaliQ608L5.
SMRiQ608L5. Positions 118-726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243233.MCA1475.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU92509; AAU92509; MCA1475.
KEGGimca:MCA1475.
PATRICi22606786. VBIMetCap22254_1505.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiRRYHHDR.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33009 / NCIMB 11132 / Bath.

Entry informationi

Entry nameiGLGB_METCA
AccessioniPrimary (citable) accession number: Q608L5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: November 23, 2004
Last modified: July 22, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.