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Q608L5 (GLGB_METCA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:MCA1475
OrganismMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]
Taxonomic identifier243233 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus

Protein attributes

Sequence length740 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7407401,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188715

Sites

Active site4091Nucleophile By similarity
Active site4621Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q608L5 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 872F7568D25B2D0B

FASTA74084,735
        10         20         30         40         50         60 
MTDSSTLAEL SPDFQRIVEA RHHDPFAVLG RHRRDNRDLI RAFLPQAEEV RVGSAGRVME 

        70         80         90        100        110        120 
RIAGTAIFEC EVEAETTDLH YRLYWTDQTG QTHSFIDPYT FPPRLSDFDL YLFGEGRHWN 

       130        140        150        160        170        180 
IYRVLGAHPH SVDGIDGILF ATWAPNAERI SVVGEFNGWD GRRHPMRVRG ASGVWELFIP 

       190        200        210        220        230        240 
ELQPGLLYKF EIRNRAHGTI HLKSDPYGRQ FELRPNTASI ITRESGYAWN DADWLAQRKD 

       250        260        270        280        290        300 
WPWLHRPLSV YEMHAGSWKR DLEGGYLNYR DLAHELVDYV KSAGFSHIEL MPVTEHPLDA 

       310        320        330        340        350        360 
SWGYQTTGYF APTSRFGTPD DFRYFVDHCH RNGIGVILDW VPAHFPKDAH GLARFDGTAL 

       370        380        390        400        410        420 
YEHEDPRLGE HRDWGTLIYN YGRNEVKNFL LGSALFWLEE FHLDGLRVDA VASMLYLDYS 

       430        440        450        460        470        480 
RQPGDWIPNK YGGNENLEAI AFLRDLNTVV HQQFPGVLVI AEESTAWPQV TRPTWTGGLG 

       490        500        510        520        530        540 
FSMKWNMGWM HDILVYMGKD PVHRHYHHDQ LTFGLLYAFT ENFVLPFSHD EVVHGKGSML 

       550        560        570        580        590        600 
AKMPGDEWRR FANLRVLYTM MFTYPGKKLL FMGCEFAQTG EWNHTTALDW PLLESNLHKG 

       610        620        630        640        650        660 
VLHLVSDLNR LYQSTSALYA YDFESQGFEW IDSHDAAQSV ISYVRRDDDS HVVVVLNFTP 

       670        680        690        700        710        720 
VPRHNYRIGV PEPVGYREVF NSDAECYGGA NLGNWEIKTE SVEWMGRAQS VVLTLPPLAG 

       730        740 
IVLAPVAPSA TPDCGTPGDE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017282 Genomic DNA. Translation: AAU92509.1.
RefSeqYP_113932.1. NC_002977.6.

3D structure databases

ProteinModelPortalQ608L5.
SMRQ608L5. Positions 118-726.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243233.MCA1475.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU92509; AAU92509; MCA1475.
GeneID3103570.
KEGGmca:MCA1475.
PATRIC22606786. VBIMetCap22254_1505.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_METCA
AccessionPrimary (citable) accession number: Q608L5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries