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Protein

Stress-induced-phosphoprotein 1

Gene

Stip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a co-chaperone for HSP90AA1. Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90.By similarity

GO - Molecular functioni

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-447041. CHL1 interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Stress-induced-phosphoprotein 1
Short name:
STI1
Short name:
mSTI1
Alternative name(s):
Hsc70/Hsp90-organizing protein
Short name:
Hop
Gene namesi
Name:Stip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:109130. Stip1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • myelin sheath Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
  • protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063731 – 543Stress-induced-phosphoprotein 1Add BLAST543

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei8N6-acetyllysineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Cross-linki123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei198PhosphothreonineBy similarity1
Cross-linki210Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei301N6-acetyllysineBy similarity1
Modified residuei312N6-acetyllysineBy similarity1
Modified residuei325N6-acetyllysineCombined sources1
Modified residuei332PhosphothreonineBy similarity1
Modified residuei344N6-acetyllysineBy similarity1
Modified residuei354PhosphotyrosineBy similarity1
Modified residuei446N6-acetyllysineBy similarity1
Modified residuei481PhosphoserineBy similarity1

Post-translational modificationi

In vitro kinase assay failed to detect phosphorylation by MAPKAPK2.

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ60864.
MaxQBiQ60864.
PaxDbiQ60864.
PeptideAtlasiQ60864.
PRIDEiQ60864.

2D gel databases

REPRODUCTION-2DPAGEIPI00121514.
Q60864.
UCD-2DPAGEQ60864.

PTM databases

iPTMnetiQ60864.
PhosphoSitePlusiQ60864.
SwissPalmiQ60864.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024966.
CleanExiMM_STIP1.
GenevisibleiQ60864. MM.

Interactioni

Subunit structurei

Forms a complex with HSPA8/HSC70, HSPCA/HSP-86 and HSPCB/HSP-84 (PubMed:8999875). Interacts with PACRG. Interacts with METTL21B. Interacts with HSP90/HSP90AA1; the interaction dissociates the PPP5C:HSP90AA1 interaction. Interacts with FLCN, FNIP1 and FNIP2. Interacts with HSPA8/HSC70 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi203539. 4 interactors.
IntActiQ60864. 5 interactors.
MINTiMINT-1869468.
STRINGi10090.ENSMUSP00000025918.

Structurei

3D structure databases

ProteinModelPortaliQ60864.
SMRiQ60864.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 37TPR 1Add BLAST34
Repeati39 – 71TPR 2Add BLAST33
Repeati73 – 105TPR 3Add BLAST33
Domaini130 – 169STI1 1Add BLAST40
Repeati225 – 258TPR 4Add BLAST34
Repeati260 – 292TPR 5Add BLAST33
Repeati300 – 333TPR 6Add BLAST34
Repeati360 – 393TPR 7Add BLAST34
Repeati395 – 427TPR 8Add BLAST33
Repeati428 – 461TPR 9Add BLAST34
Domaini492 – 531STI1 2Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi222 – 239Bipartite nuclear localization signalSequence analysisAdd BLAST18

Domaini

The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90 (By similarity).By similarity

Sequence similaritiesi

Contains 2 STI1 domains.Curated
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0548. Eukaryota.
ENOG410XTCJ. LUCA.
GeneTreeiENSGT00840000129761.
HOGENOMiHOG000186562.
HOVERGENiHBG057820.
InParanoidiQ60864.
KOiK09553.
OMAiSKCRELC.
OrthoDBiEOG091G0E5W.
PhylomeDBiQ60864.
TreeFamiTF300478.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR006636. STI1_HS-bd.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 1 hit.
PF13414. TPR_11. 2 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00727. STI1. 2 hits.
SM00028. TPR. 9 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 9 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQVNELKEK GNKALSAGNI DDALQCYSEA IKLDPQNHVL YSNRSAAYAK
60 70 80 90 100
KGDYQKAYED GCKTVDLKPD WGKGYSRKAA ALEFLNRFEE AKRTYEEGLK
110 120 130 140 150
HEANNLQLKE GLQNMEARLA ERKFMNPFNL PNLYQKLEND PRTRSLLSDP
160 170 180 190 200
TYRELIEQLQ NKPSDLGTKL QDPRVMTTLS VLLGVDLGSM DEEEEAATPP
210 220 230 240 250
PPPPPKKEPK PEPMEEDLPE NKKQALKEKE LGNDAYKKKD FDKALKHYDR
260 270 280 290 300
AKELDPTNMT YITNQAAVHF EKGDYNKCRE LCEKAIEVGR ENREDYRQIA
310 320 330 340 350
KAYARIGNSY FKEEKYKDAI HFYNKSLAEH RTPDVLKKCQ QAEKILKEQE
360 370 380 390 400
RLAYINPDLA LEEKNKGNEC FQKGDYPQAM KHYTEAIKRN PRDAKLYSNR
410 420 430 440 450
AACYTKLLEF QLALKDCEEC IQLEPTFIKG YTRKAAALEA MKDYTKAMDV
460 470 480 490 500
YQKALDLDSS CKEAADGYQR CMMAQYNRHD SPEDVKRRAM ADPEVQQIMS
510 520 530 540
DPAMRLILEQ MQKDPQALSE HLKNPVIAQK IQKLMDVGLI AIR
Length:543
Mass (Da):62,582
Last modified:November 1, 1996 - v1
Checksum:iB737FBA92B198D6C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15L → P in BAC36100 (PubMed:16141072).Curated1
Sequence conflicti106L → V in AAH03794 (PubMed:15489334).Curated1
Sequence conflicti274 – 279DYNKCR → RLYKCT in BAC36100 (PubMed:16141072).Curated6
Sequence conflicti288V → S in BAC36100 (PubMed:16141072).Curated1
Sequence conflicti305 – 310RIGNSY → PNWQFL in BAC36100 (PubMed:16141072).Curated6
Sequence conflicti316 – 317YK → VQ in BAC36100 (PubMed:16141072).Curated2
Sequence conflicti333 – 347PDVLK…EKILK → QMCSRSASSQRNSE in BAC36100 (PubMed:16141072).CuratedAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27830 mRNA. Translation: AAC53267.1.
AK075988 mRNA. Translation: BAC36100.1.
AK088494 mRNA. Translation: BAC40389.1.
AK149493 mRNA. Translation: BAE28916.1.
AK161645 mRNA. Translation: BAE36509.1.
AK167273 mRNA. Translation: BAE39385.1.
BC003794 mRNA. Translation: AAH03794.1.
CCDSiCCDS37901.1.
RefSeqiNP_058017.1. NM_016737.2.
UniGeneiMm.258633.

Genome annotation databases

EnsembliENSMUST00000025918; ENSMUSP00000025918; ENSMUSG00000024966.
GeneIDi20867.
KEGGimmu:20867.
UCSCiuc008gke.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27830 mRNA. Translation: AAC53267.1.
AK075988 mRNA. Translation: BAC36100.1.
AK088494 mRNA. Translation: BAC40389.1.
AK149493 mRNA. Translation: BAE28916.1.
AK161645 mRNA. Translation: BAE36509.1.
AK167273 mRNA. Translation: BAE39385.1.
BC003794 mRNA. Translation: AAH03794.1.
CCDSiCCDS37901.1.
RefSeqiNP_058017.1. NM_016737.2.
UniGeneiMm.258633.

3D structure databases

ProteinModelPortaliQ60864.
SMRiQ60864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203539. 4 interactors.
IntActiQ60864. 5 interactors.
MINTiMINT-1869468.
STRINGi10090.ENSMUSP00000025918.

PTM databases

iPTMnetiQ60864.
PhosphoSitePlusiQ60864.
SwissPalmiQ60864.

2D gel databases

REPRODUCTION-2DPAGEIPI00121514.
Q60864.
UCD-2DPAGEQ60864.

Proteomic databases

EPDiQ60864.
MaxQBiQ60864.
PaxDbiQ60864.
PeptideAtlasiQ60864.
PRIDEiQ60864.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025918; ENSMUSP00000025918; ENSMUSG00000024966.
GeneIDi20867.
KEGGimmu:20867.
UCSCiuc008gke.2. mouse.

Organism-specific databases

CTDi10963.
MGIiMGI:109130. Stip1.

Phylogenomic databases

eggNOGiKOG0548. Eukaryota.
ENOG410XTCJ. LUCA.
GeneTreeiENSGT00840000129761.
HOGENOMiHOG000186562.
HOVERGENiHBG057820.
InParanoidiQ60864.
KOiK09553.
OMAiSKCRELC.
OrthoDBiEOG091G0E5W.
PhylomeDBiQ60864.
TreeFamiTF300478.

Enzyme and pathway databases

ReactomeiR-MMU-447041. CHL1 interactions.

Miscellaneous databases

ChiTaRSiStip1. mouse.
PROiQ60864.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024966.
CleanExiMM_STIP1.
GenevisibleiQ60864. MM.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR006636. STI1_HS-bd.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 1 hit.
PF13414. TPR_11. 2 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00727. STI1. 2 hits.
SM00028. TPR. 9 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 9 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTIP1_MOUSE
AccessioniPrimary (citable) accession number: Q60864
Secondary accession number(s): Q3TT16, Q8BPH3, Q99L66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.