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Q60862

- ORC2_MOUSE

UniProt

Q60862 - ORC2_MOUSE

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Protein
Origin recognition complex subunit 2
Gene
Orc2, Orc2l
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication By similarity. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3 By similarity.

GO - Molecular functioni

  1. DNA replication origin binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

ReactomeiREACT_204269. E2F-enabled inhibition of pre-replication complex formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 2
Gene namesi
Name:Orc2
Synonyms:Orc2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1328306. Orc2.

Subcellular locationi

GO - Cellular componenti

  1. chromatin Source: MGI
  2. condensed chromosome inner kinetochore Source: MGI
  3. heterochromatin Source: MGI
  4. nuclear origin of replication recognition complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Origin recognition complex subunit 2
PRO_0000127076Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161Phosphothreonine By similarity
Modified residuei225 – 2251Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60862.
PRIDEiQ60862.

PTM databases

PhosphoSiteiQ60862.

Expressioni

Gene expression databases

ArrayExpressiQ60862.
BgeeiQ60862.
GenevestigatoriQ60862.

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase By similarity. Interacts with MCM10 By similarity. Interacts with LRWD1 throughout the cell cycle; this interaction, wich occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1 ubiquitination and hence stabilizes the protein By similarity. Interacts with DBF4.1 Publication

Protein-protein interaction databases

BioGridi201975. 4 interactions.
IntActiQ60862. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ60862.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 100100Involved in LRWD1-binding By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the ORC2 family.

Phylogenomic databases

eggNOGiCOG5575.
GeneTreeiENSGT00390000015228.
HOGENOMiHOG000045588.
HOVERGENiHBG007874.
InParanoidiQ60862.
KOiK02604.
OMAiSQMLRGE.
OrthoDBiEOG7NPFT1.
PhylomeDBiQ60862.
TreeFamiTF101092.

Family and domain databases

InterProiIPR007220. ORC2.
[Graphical view]
PANTHERiPTHR14052. PTHR14052. 1 hit.
PfamiPF04084. ORC2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60862-1 [UniParc]FASTAAdd to Basket

« Hide

MSTLQLKETK VPSVQFVGDD DVLSHILDRE GGTKLKKEKA QLLVNPQKVI    50
KKADCELEKS DLEVLEDQNY VKVLGRNIQE SLGNGSAKDG RNKVYSFQQR 100
KHPEEMTKLA LELAKTSGKK DPLDSNDPEI TKNIAQKSKG HSTSEKAPLV 150
NNNKTEFLST QPHNLRKRII ASRSHYDSES EYSASSSEDD EEATKDEEED 200
TNVARLSQKS QGQNRLLPAP VSKETLPKKK KRDKASDLVE EYFEAHSSSK 250
VLTSDRTLQR LRRARVDQKT LHNLLRKFVP SFSAEIERLN QQHEKLFHKW 300
MLQLHLGFNI VLYGLGSKRD LLEKFRTTML QDSIHVVING YFPGVSVKSI 350
LNSITEDVLS HVGTFQSVLD QRDWIINRFK EDSSLELFLL IHNLDSQMLR 400
GDNSQQILGQ LSSLHNVYLI ASIDHLNAPL MWDHAKQSLY NWLWYETTTY 450
SPYTEETSYE NSLLVKQSGS LPLSSLIHVL RSLTPNARGI FRLLMKFQLD 500
NQDSPSYIGL SFQDFYQQCR EAFLVNSDLT LRAQLTEFRD HKLIRTKKGT 550
DGVEYLLIPV DSGILADFLE KEEEEA 576
Length:576
Mass (Da):65,893
Last modified:November 1, 1997 - v1
Checksum:iA18BA2D18E72553B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U27457 mRNA. Translation: AAB33994.1.
BC015257 mRNA. Translation: AAH15257.1.
CCDSiCCDS14975.1.
RefSeqiNP_001258455.1. NM_001271526.1.
NP_032791.1. NM_008765.3.
UniGeneiMm.3411.

Genome annotation databases

EnsembliENSMUST00000027198; ENSMUSP00000027198; ENSMUSG00000026037.
GeneIDi18393.
KEGGimmu:18393.
UCSCiuc007bcd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U27457 mRNA. Translation: AAB33994.1 .
BC015257 mRNA. Translation: AAH15257.1 .
CCDSi CCDS14975.1.
RefSeqi NP_001258455.1. NM_001271526.1.
NP_032791.1. NM_008765.3.
UniGenei Mm.3411.

3D structure databases

ProteinModelPortali Q60862.
ModBasei Search...

Protein-protein interaction databases

BioGridi 201975. 4 interactions.
IntActi Q60862. 1 interaction.

PTM databases

PhosphoSitei Q60862.

Proteomic databases

PaxDbi Q60862.
PRIDEi Q60862.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027198 ; ENSMUSP00000027198 ; ENSMUSG00000026037 .
GeneIDi 18393.
KEGGi mmu:18393.
UCSCi uc007bcd.1. mouse.

Organism-specific databases

CTDi 4999.
MGIi MGI:1328306. Orc2.

Phylogenomic databases

eggNOGi COG5575.
GeneTreei ENSGT00390000015228.
HOGENOMi HOG000045588.
HOVERGENi HBG007874.
InParanoidi Q60862.
KOi K02604.
OMAi SQMLRGE.
OrthoDBi EOG7NPFT1.
PhylomeDBi Q60862.
TreeFami TF101092.

Enzyme and pathway databases

Reactomei REACT_204269. E2F-enabled inhibition of pre-replication complex formation.

Miscellaneous databases

ChiTaRSi ORC2. mouse.
NextBioi 294002.
PROi Q60862.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q60862.
Bgeei Q60862.
Genevestigatori Q60862.

Family and domain databases

InterProi IPR007220. ORC2.
[Graphical view ]
PANTHERi PTHR14052. PTHR14052. 1 hit.
Pfami PF04084. ORC2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L."
    Takahara K., Bong M., Brevard R., Eddy R.L., Haley L.L., Sait S.J., Shows T.B., Hoffman G.G., Greenspan D.S.
    Genomics 31:119-122(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  3. "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells."
    Kneissl M., Puetter V., Szalay A.A., Grummt F.
    J. Mol. Biol. 327:111-128(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DBF4.

Entry informationi

Entry nameiORC2_MOUSE
AccessioniPrimary (citable) accession number: Q60862
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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