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Protein

Origin recognition complex subunit 2

Gene

Orc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3 (By similarity).By similarity

GO - Molecular functioni

  1. DNA replication origin binding Source: MGI

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

ReactomeiREACT_204269. E2F-enabled inhibition of pre-replication complex formation.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_244558. CDT1 association with the CDC6:ORC:origin complex.
REACT_245230. Orc1 removal from chromatin.
REACT_247969. Assembly of the ORC complex at the origin of replication.
REACT_254983. Activation of the pre-replicative complex.
REACT_259974. CDC6 association with the ORC:origin complex.
REACT_262002. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 2
Gene namesi
Name:Orc2
Synonyms:Orc2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1328306. Orc2.

Subcellular locationi

GO - Cellular componenti

  1. chromatin Source: MGI
  2. condensed chromosome inner kinetochore Source: MGI
  3. heterochromatin Source: MGI
  4. membrane Source: MGI
  5. nuclear origin of replication recognition complex Source: MGI
  6. nucleus Source: MGI
  7. origin recognition complex Source: MGI
  8. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Origin recognition complex subunit 2PRO_0000127076Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161PhosphothreonineBy similarity
Modified residuei225 – 2251PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ60862.
PaxDbiQ60862.
PRIDEiQ60862.

PTM databases

PhosphoSiteiQ60862.

Expressioni

Gene expression databases

BgeeiQ60862.
ExpressionAtlasiQ60862. baseline and differential.
GenevestigatoriQ60862.

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase (By similarity). Interacts with MCM10 (By similarity). Interacts with LRWD1 throughout the cell cycle; this interaction, wich occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1 ubiquitination and hence stabilizes the protein (By similarity). Interacts with DBF4.By similarity1 Publication

Protein-protein interaction databases

BioGridi201975. 4 interactions.
IntActiQ60862. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ60862.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 100100Involved in LRWD1-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the ORC2 family.Curated

Phylogenomic databases

eggNOGiCOG5575.
GeneTreeiENSGT00390000015228.
HOGENOMiHOG000045588.
HOVERGENiHBG007874.
InParanoidiQ60862.
KOiK02604.
OMAiPVCKETP.
OrthoDBiEOG7NPFT1.
PhylomeDBiQ60862.
TreeFamiTF101092.

Family and domain databases

InterProiIPR007220. ORC2.
[Graphical view]
PANTHERiPTHR14052. PTHR14052. 1 hit.
PfamiPF04084. ORC2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60862-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTLQLKETK VPSVQFVGDD DVLSHILDRE GGTKLKKEKA QLLVNPQKVI
60 70 80 90 100
KKADCELEKS DLEVLEDQNY VKVLGRNIQE SLGNGSAKDG RNKVYSFQQR
110 120 130 140 150
KHPEEMTKLA LELAKTSGKK DPLDSNDPEI TKNIAQKSKG HSTSEKAPLV
160 170 180 190 200
NNNKTEFLST QPHNLRKRII ASRSHYDSES EYSASSSEDD EEATKDEEED
210 220 230 240 250
TNVARLSQKS QGQNRLLPAP VSKETLPKKK KRDKASDLVE EYFEAHSSSK
260 270 280 290 300
VLTSDRTLQR LRRARVDQKT LHNLLRKFVP SFSAEIERLN QQHEKLFHKW
310 320 330 340 350
MLQLHLGFNI VLYGLGSKRD LLEKFRTTML QDSIHVVING YFPGVSVKSI
360 370 380 390 400
LNSITEDVLS HVGTFQSVLD QRDWIINRFK EDSSLELFLL IHNLDSQMLR
410 420 430 440 450
GDNSQQILGQ LSSLHNVYLI ASIDHLNAPL MWDHAKQSLY NWLWYETTTY
460 470 480 490 500
SPYTEETSYE NSLLVKQSGS LPLSSLIHVL RSLTPNARGI FRLLMKFQLD
510 520 530 540 550
NQDSPSYIGL SFQDFYQQCR EAFLVNSDLT LRAQLTEFRD HKLIRTKKGT
560 570
DGVEYLLIPV DSGILADFLE KEEEEA
Length:576
Mass (Da):65,893
Last modified:November 1, 1997 - v1
Checksum:iA18BA2D18E72553B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27457 mRNA. Translation: AAB33994.1.
BC015257 mRNA. Translation: AAH15257.1.
CCDSiCCDS14975.1.
RefSeqiNP_001258455.1. NM_001271526.1.
NP_032791.1. NM_008765.3.
UniGeneiMm.3411.

Genome annotation databases

EnsembliENSMUST00000027198; ENSMUSP00000027198; ENSMUSG00000026037.
GeneIDi18393.
KEGGimmu:18393.
UCSCiuc007bcd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27457 mRNA. Translation: AAB33994.1.
BC015257 mRNA. Translation: AAH15257.1.
CCDSiCCDS14975.1.
RefSeqiNP_001258455.1. NM_001271526.1.
NP_032791.1. NM_008765.3.
UniGeneiMm.3411.

3D structure databases

ProteinModelPortaliQ60862.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201975. 4 interactions.
IntActiQ60862. 1 interaction.

PTM databases

PhosphoSiteiQ60862.

Proteomic databases

MaxQBiQ60862.
PaxDbiQ60862.
PRIDEiQ60862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027198; ENSMUSP00000027198; ENSMUSG00000026037.
GeneIDi18393.
KEGGimmu:18393.
UCSCiuc007bcd.1. mouse.

Organism-specific databases

CTDi4999.
MGIiMGI:1328306. Orc2.

Phylogenomic databases

eggNOGiCOG5575.
GeneTreeiENSGT00390000015228.
HOGENOMiHOG000045588.
HOVERGENiHBG007874.
InParanoidiQ60862.
KOiK02604.
OMAiPVCKETP.
OrthoDBiEOG7NPFT1.
PhylomeDBiQ60862.
TreeFamiTF101092.

Enzyme and pathway databases

ReactomeiREACT_204269. E2F-enabled inhibition of pre-replication complex formation.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_244558. CDT1 association with the CDC6:ORC:origin complex.
REACT_245230. Orc1 removal from chromatin.
REACT_247969. Assembly of the ORC complex at the origin of replication.
REACT_254983. Activation of the pre-replicative complex.
REACT_259974. CDC6 association with the ORC:origin complex.
REACT_262002. Association of licensing factors with the pre-replicative complex.

Miscellaneous databases

ChiTaRSiOrc2. mouse.
NextBioi294002.
PROiQ60862.
SOURCEiSearch...

Gene expression databases

BgeeiQ60862.
ExpressionAtlasiQ60862. baseline and differential.
GenevestigatoriQ60862.

Family and domain databases

InterProiIPR007220. ORC2.
[Graphical view]
PANTHERiPTHR14052. PTHR14052. 1 hit.
PfamiPF04084. ORC2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L."
    Takahara K., Bong M., Brevard R., Eddy R.L., Haley L.L., Sait S.J., Shows T.B., Hoffman G.G., Greenspan D.S.
    Genomics 31:119-122(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  3. "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells."
    Kneissl M., Puetter V., Szalay A.A., Grummt F.
    J. Mol. Biol. 327:111-128(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DBF4.

Entry informationi

Entry nameiORC2_MOUSE
AccessioniPrimary (citable) accession number: Q60862
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 4, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.