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Q60862

- ORC2_MOUSE

UniProt

Q60862 - ORC2_MOUSE

Protein

Origin recognition complex subunit 2

Gene

Orc2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication By similarity. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3 By similarity.By similarity

    GO - Molecular functioni

    1. DNA replication origin binding Source: MGI

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Biological processi

    DNA replication

    Enzyme and pathway databases

    ReactomeiREACT_204269. E2F-enabled inhibition of pre-replication complex formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Origin recognition complex subunit 2
    Gene namesi
    Name:Orc2
    Synonyms:Orc2l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1328306. Orc2.

    Subcellular locationi

    GO - Cellular componenti

    1. chromatin Source: MGI
    2. condensed chromosome inner kinetochore Source: MGI
    3. heterochromatin Source: MGI
    4. nuclear origin of replication recognition complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 576576Origin recognition complex subunit 2PRO_0000127076Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei116 – 1161PhosphothreonineBy similarity
    Modified residuei225 – 2251PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ60862.
    PRIDEiQ60862.

    PTM databases

    PhosphoSiteiQ60862.

    Expressioni

    Gene expression databases

    ArrayExpressiQ60862.
    BgeeiQ60862.
    GenevestigatoriQ60862.

    Interactioni

    Subunit structurei

    Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase By similarity. Interacts with MCM10 By similarity. Interacts with LRWD1 throughout the cell cycle; this interaction, wich occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1 ubiquitination and hence stabilizes the protein By similarity. Interacts with DBF4.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi201975. 4 interactions.
    IntActiQ60862. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60862.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1 – 100100Involved in LRWD1-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ORC2 family.Curated

    Phylogenomic databases

    eggNOGiCOG5575.
    GeneTreeiENSGT00390000015228.
    HOGENOMiHOG000045588.
    HOVERGENiHBG007874.
    InParanoidiQ60862.
    KOiK02604.
    OMAiSQMLRGE.
    OrthoDBiEOG7NPFT1.
    PhylomeDBiQ60862.
    TreeFamiTF101092.

    Family and domain databases

    InterProiIPR007220. ORC2.
    [Graphical view]
    PANTHERiPTHR14052. PTHR14052. 1 hit.
    PfamiPF04084. ORC2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60862-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTLQLKETK VPSVQFVGDD DVLSHILDRE GGTKLKKEKA QLLVNPQKVI    50
    KKADCELEKS DLEVLEDQNY VKVLGRNIQE SLGNGSAKDG RNKVYSFQQR 100
    KHPEEMTKLA LELAKTSGKK DPLDSNDPEI TKNIAQKSKG HSTSEKAPLV 150
    NNNKTEFLST QPHNLRKRII ASRSHYDSES EYSASSSEDD EEATKDEEED 200
    TNVARLSQKS QGQNRLLPAP VSKETLPKKK KRDKASDLVE EYFEAHSSSK 250
    VLTSDRTLQR LRRARVDQKT LHNLLRKFVP SFSAEIERLN QQHEKLFHKW 300
    MLQLHLGFNI VLYGLGSKRD LLEKFRTTML QDSIHVVING YFPGVSVKSI 350
    LNSITEDVLS HVGTFQSVLD QRDWIINRFK EDSSLELFLL IHNLDSQMLR 400
    GDNSQQILGQ LSSLHNVYLI ASIDHLNAPL MWDHAKQSLY NWLWYETTTY 450
    SPYTEETSYE NSLLVKQSGS LPLSSLIHVL RSLTPNARGI FRLLMKFQLD 500
    NQDSPSYIGL SFQDFYQQCR EAFLVNSDLT LRAQLTEFRD HKLIRTKKGT 550
    DGVEYLLIPV DSGILADFLE KEEEEA 576
    Length:576
    Mass (Da):65,893
    Last modified:November 1, 1997 - v1
    Checksum:iA18BA2D18E72553B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27457 mRNA. Translation: AAB33994.1.
    BC015257 mRNA. Translation: AAH15257.1.
    CCDSiCCDS14975.1.
    RefSeqiNP_001258455.1. NM_001271526.1.
    NP_032791.1. NM_008765.3.
    UniGeneiMm.3411.

    Genome annotation databases

    EnsembliENSMUST00000027198; ENSMUSP00000027198; ENSMUSG00000026037.
    GeneIDi18393.
    KEGGimmu:18393.
    UCSCiuc007bcd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27457 mRNA. Translation: AAB33994.1 .
    BC015257 mRNA. Translation: AAH15257.1 .
    CCDSi CCDS14975.1.
    RefSeqi NP_001258455.1. NM_001271526.1.
    NP_032791.1. NM_008765.3.
    UniGenei Mm.3411.

    3D structure databases

    ProteinModelPortali Q60862.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201975. 4 interactions.
    IntActi Q60862. 1 interaction.

    PTM databases

    PhosphoSitei Q60862.

    Proteomic databases

    PaxDbi Q60862.
    PRIDEi Q60862.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027198 ; ENSMUSP00000027198 ; ENSMUSG00000026037 .
    GeneIDi 18393.
    KEGGi mmu:18393.
    UCSCi uc007bcd.1. mouse.

    Organism-specific databases

    CTDi 4999.
    MGIi MGI:1328306. Orc2.

    Phylogenomic databases

    eggNOGi COG5575.
    GeneTreei ENSGT00390000015228.
    HOGENOMi HOG000045588.
    HOVERGENi HBG007874.
    InParanoidi Q60862.
    KOi K02604.
    OMAi SQMLRGE.
    OrthoDBi EOG7NPFT1.
    PhylomeDBi Q60862.
    TreeFami TF101092.

    Enzyme and pathway databases

    Reactomei REACT_204269. E2F-enabled inhibition of pre-replication complex formation.

    Miscellaneous databases

    ChiTaRSi ORC2. mouse.
    NextBioi 294002.
    PROi Q60862.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q60862.
    Bgeei Q60862.
    Genevestigatori Q60862.

    Family and domain databases

    InterProi IPR007220. ORC2.
    [Graphical view ]
    PANTHERi PTHR14052. PTHR14052. 1 hit.
    Pfami PF04084. ORC2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L."
      Takahara K., Bong M., Brevard R., Eddy R.L., Haley L.L., Sait S.J., Shows T.B., Hoffman G.G., Greenspan D.S.
      Genomics 31:119-122(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    3. "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells."
      Kneissl M., Puetter V., Szalay A.A., Grummt F.
      J. Mol. Biol. 327:111-128(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DBF4.

    Entry informationi

    Entry nameiORC2_MOUSE
    AccessioniPrimary (citable) accession number: Q60862
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3