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Q60862 (ORC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Origin recognition complex subunit 2
Gene names
Name:Orc2
Synonyms:Orc2l
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication By similarity. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3 By similarity.

Subunit structure

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase By similarity. Interacts with MCM10 By similarity. Interacts with LRWD1 throughout the cell cycle; this interaction, wich occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1 ubiquitination and hence stabilizes the protein By similarity. Interacts with DBF4. Ref.3

Subcellular location

Nucleus.

Sequence similarities

Belongs to the ORC2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Origin recognition complex subunit 2
PRO_0000127076

Regions

Repeat1 – 100100Involved in LRWD1-binding By similarity

Amino acid modifications

Modified residue1161Phosphothreonine By similarity
Modified residue2251Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60862 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: A18BA2D18E72553B

FASTA57665,893
        10         20         30         40         50         60 
MSTLQLKETK VPSVQFVGDD DVLSHILDRE GGTKLKKEKA QLLVNPQKVI KKADCELEKS 

        70         80         90        100        110        120 
DLEVLEDQNY VKVLGRNIQE SLGNGSAKDG RNKVYSFQQR KHPEEMTKLA LELAKTSGKK 

       130        140        150        160        170        180 
DPLDSNDPEI TKNIAQKSKG HSTSEKAPLV NNNKTEFLST QPHNLRKRII ASRSHYDSES 

       190        200        210        220        230        240 
EYSASSSEDD EEATKDEEED TNVARLSQKS QGQNRLLPAP VSKETLPKKK KRDKASDLVE 

       250        260        270        280        290        300 
EYFEAHSSSK VLTSDRTLQR LRRARVDQKT LHNLLRKFVP SFSAEIERLN QQHEKLFHKW 

       310        320        330        340        350        360 
MLQLHLGFNI VLYGLGSKRD LLEKFRTTML QDSIHVVING YFPGVSVKSI LNSITEDVLS 

       370        380        390        400        410        420 
HVGTFQSVLD QRDWIINRFK EDSSLELFLL IHNLDSQMLR GDNSQQILGQ LSSLHNVYLI 

       430        440        450        460        470        480 
ASIDHLNAPL MWDHAKQSLY NWLWYETTTY SPYTEETSYE NSLLVKQSGS LPLSSLIHVL 

       490        500        510        520        530        540 
RSLTPNARGI FRLLMKFQLD NQDSPSYIGL SFQDFYQQCR EAFLVNSDLT LRAQLTEFRD 

       550        560        570 
HKLIRTKKGT DGVEYLLIPV DSGILADFLE KEEEEA 

« Hide

References

« Hide 'large scale' references
[1]"Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L."
Takahara K., Bong M., Brevard R., Eddy R.L., Haley L.L., Sait S.J., Shows T.B., Hoffman G.G., Greenspan D.S.
Genomics 31:119-122(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[3]"Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells."
Kneissl M., Puetter V., Szalay A.A., Grummt F.
J. Mol. Biol. 327:111-128(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DBF4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U27457 mRNA. Translation: AAB33994.1.
BC015257 mRNA. Translation: AAH15257.1.
CCDSCCDS14975.1.
RefSeqNP_001258455.1. NM_001271526.1.
NP_032791.1. NM_008765.3.
UniGeneMm.3411.

3D structure databases

ProteinModelPortalQ60862.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201975. 4 interactions.
IntActQ60862. 1 interaction.

PTM databases

PhosphoSiteQ60862.

Proteomic databases

PaxDbQ60862.
PRIDEQ60862.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027198; ENSMUSP00000027198; ENSMUSG00000026037.
GeneID18393.
KEGGmmu:18393.
UCSCuc007bcd.1. mouse.

Organism-specific databases

CTD4999.
MGIMGI:1328306. Orc2.

Phylogenomic databases

eggNOGCOG5575.
GeneTreeENSGT00390000015228.
HOGENOMHOG000045588.
HOVERGENHBG007874.
InParanoidQ60862.
KOK02604.
OMASQMLRGE.
OrthoDBEOG7NPFT1.
PhylomeDBQ60862.
TreeFamTF101092.

Gene expression databases

ArrayExpressQ60862.
BgeeQ60862.
GenevestigatorQ60862.

Family and domain databases

InterProIPR007220. ORC2.
[Graphical view]
PANTHERPTHR14052. PTHR14052. 1 hit.
PfamPF04084. ORC2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSORC2. mouse.
NextBio294002.
PROQ60862.
SOURCESearch...

Entry information

Entry nameORC2_MOUSE
AccessionPrimary (citable) accession number: Q60862
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot