Q60860 (LTC4S_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leukotriene C4 synthase
Short name=LTC4 synthase
EC=4.4.1.20

Alternative name(s):
Leukotriene-C(4) synthase

Gene names

Name:

Ltc4s

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.
Catalytic activity	Leukotriene C4 = leukotriene A4 + glutathione.
Subunit structure	Homotrimer. Interacts with ALOX5AP and ALOX5 By similarity.
Subcellular location	Nucleus outer membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Widely expressed. Ref.4
Sequence similarities	Belongs to the MAPEG family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Endoplasmic reticulum Membrane Nucleus
Domain	Transmembrane Transmembrane helix
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glutathione biosynthetic process Inferred from Biological aspect of Ancestor. Source: RefGenome leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW leukotriene metabolic process Inferred from direct assay Ref.4. Source: MGI positive regulation of catalytic activity Inferred from electronic annotation. Source: GOC
Cellular_component	endoplasmic reticulum Inferred from Biological aspect of Ancestor. Source: RefGenome endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from sequence or structural similarity. Source: UniProtKB nuclear outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	enzyme activator activity Inferred from electronic annotation. Source: InterPro glutathione peroxidase activity Inferred from Biological aspect of Ancestor. Source: RefGenome glutathione transferase activity Inferred from Biological aspect of Ancestor. Source: RefGenome leukotriene-C4 synthase activity Inferred from direct assay Ref.4. Source: MGI lipid binding Inferred from direct assay Ref.4. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 150	150	Leukotriene C4 synthase		PRO_0000217749
Regions
	Topological domain	1 – 6	6	Cytoplasmic By similarity
	Transmembrane	7 – 27	21	Helical; By similarity
	Topological domain	28 – 48	21	Lumenal By similarity
	Transmembrane	49 – 69	21	Helical; By similarity
	Topological domain	70 – 73	4	Cytoplasmic By similarity
	Transmembrane	74 – 94	21	Helical; By similarity
	Topological domain	95 – 104	10	Lumenal By similarity
	Transmembrane	105 – 124	20	Helical; By similarity
	Topological domain	125 – 150	26	Cytoplasmic By similarity
	Region	51 – 55	5	Glutathione binding By similarity
	Region	58 – 59	2	Glutathione binding By similarity
	Region	93 – 97	5	Glutathione binding By similarity
Sites
	Active site	31	1	Proton donor By similarity
	Active site	104	1	Proton acceptor By similarity
	Binding site	30	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60860 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 120ABC53892189C0	FASTA	150	16,814
	10 20 30 40 50 60 MKDEVALLAT VTLVGVLLQA YFSLQVISAR RAFHVSPPLT SGPPEFERVF RAQVNCSEYF 70 80 90 100 110 120 PLFLATLWVA GIFFHEGAAA LCGLFYLFAR LRYFQGYARS AQLRLTPLYA SARALWLLVA 130 140 150 MAALGLLVHF LPGTLRTALF RWLQMLLPMA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the gene for mouse leukotriene-C4 synthase." Penrose J.F., Baldasaro M.H., Webster M., Xu K., Austen K.F., Lam B.K. Eur. J. Biochem. 248:807-813(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Two step purification of human and murine leukotriene C4 synthase." Goppelt-Struebe M. Biochim. Biophys. Acta 1256:257-261(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-27. Tissue: Mast cell.
[4]	"Molecular cloning, expression and characterization of mouse leukotriene C4 synthase." Lam B.K., Penrose J.F., Rokach J., Xu K., Baldasaro M.H., Austen K.F. Eur. J. Biochem. 238:606-612(1996) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27195 mRNA. Translation: AAA75042.1. AL627187 Genomic DNA. Translation: CAI25121.1.
IPI	IPI00121507.
PIR	S68961.
RefSeq	NP_032547.1. NM_008521.1.
UniGene	Mm.245151.
3D structure databases
ProteinModelPortal	Q60860.
SMR	Q60860. Positions 1-111.
ModBase	Search...
PTM databases
PhosphoSite	Q60860.
Proteomic databases
PRIDE	Q60860.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000102772; ENSMUSP00000099833; ENSMUSG00000020377.
GeneID	17001.
KEGG	mmu:17001.
UCSC	uc007isa.1. mouse.
Organism-specific databases
CTD	4056.
MGI	MGI:107498. Ltc4s.
Phylogenomic databases
GeneTree	ENSGT00430000030964.
HOGENOM	HOG000116372.
HOVERGEN	HBG105513.
InParanoid	Q5SVR7.
KO	K00807.
OrthoDB	EOG4V1729.
Enzyme and pathway databases
BRENDA	4.4.1.20. 3474.
Gene expression databases
ArrayExpress	Q60860.
Bgee	Q60860.
CleanEx	MM_LTC4S.
Genevestigator	Q60860.
GermOnline	ENSMUSG00000020377. Mus musculus.
Family and domain databases
Gene3D	1.20.120.550. 1 hit.
InterPro	IPR001446. 5_LipOase_AP. IPR018295. FLAP/GST2/LTC4S_CS. IPR023352. MAPEG-like_dom. IPR001129. Membr-assoc_MAPEG. [Graphical view]
Pfam	PF01124. MAPEG. 1 hit. [Graphical view]
PRINTS	PR00488. 5LPOXGNASEAP.
PROSITE	PS01297. FLAP_GST2_LTC4S. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291112.
SOURCE	Search...

Entry information

Entry name

LTC4S_MOUSE

Accession

Primary (citable) accession number: Q60860
Secondary accession number(s): Q5SVR7, Q9QVS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program