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Protein

Leukotriene C4 synthase

Gene

Ltc4s

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.

Catalytic activityi

Leukotriene C4 = leukotriene A4 + glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30GlutathioneBy similarity1
Active sitei31Proton donorBy similarity1
Active sitei104Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • leukotriene biosynthetic process Source: GO_Central
  • leukotriene metabolic process Source: MGI

Keywordsi

Molecular functionLyase
Biological processLeukotriene biosynthesis

Enzyme and pathway databases

BRENDAi4.4.1.20 3474
ReactomeiR-MMU-2142688 Synthesis of 5-eicosatetraenoic acids
R-MMU-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-MMU-2142700 Synthesis of Lipoxins (LX)
R-MMU-9026762 Biosynthesis of maresin conjugates in tissue regeneration (MCTR)
R-MMU-9026766 Biosynthesis of protectin and resolvin conjugates in tissue regeneration (PCTR and RCTR)

Chemistry databases

SwissLipidsiSLP:000001453

Names & Taxonomyi

Protein namesi
Recommended name:
Leukotriene C4 synthase (EC:4.4.1.20)
Short name:
LTC4 synthase
Alternative name(s):
Leukotriene-C(4) synthase
Gene namesi
Name:Ltc4s
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107498 Ltc4s

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicBy similarity6
Transmembranei7 – 27HelicalBy similarityAdd BLAST21
Topological domaini28 – 48LumenalBy similarityAdd BLAST21
Transmembranei49 – 69HelicalBy similarityAdd BLAST21
Topological domaini70 – 73CytoplasmicBy similarity4
Transmembranei74 – 94HelicalBy similarityAdd BLAST21
Topological domaini95 – 104LumenalBy similarity10
Transmembranei105 – 124HelicalBy similarityAdd BLAST20
Topological domaini125 – 150CytoplasmicBy similarityAdd BLAST26

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177491 – 150Leukotriene C4 synthaseAdd BLAST150

Proteomic databases

MaxQBiQ60860
PaxDbiQ60860
PeptideAtlasiQ60860
PRIDEiQ60860

PTM databases

iPTMnetiQ60860
PhosphoSitePlusiQ60860

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000020377
CleanExiMM_LTC4S
ExpressionAtlasiQ60860 baseline and differential
GenevisibleiQ60860 MM

Interactioni

Subunit structurei

Homotrimer. Interacts with ALOX5AP and ALOX5 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099833

Structurei

Secondary structure

1150
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 5Combined sources4
Helixi6 – 32Combined sources27
Helixi44 – 73Combined sources30
Helixi76 – 99Combined sources24
Helixi102 – 104Combined sources3
Helixi105 – 141Combined sources37

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NTAX-ray2.70A2-150[»]
4NTBX-ray2.70A2-150[»]
4NTFX-ray2.65A2-150[»]
ProteinModelPortaliQ60860
SMRiQ60860
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 55Glutathione bindingBy similarity5
Regioni58 – 59Glutathione bindingBy similarity2
Regioni93 – 97Glutathione bindingBy similarity5

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IX5F Eukaryota
ENOG4111JV7 LUCA
GeneTreeiENSGT00430000030964
HOGENOMiHOG000116372
HOVERGENiHBG105513
InParanoidiQ60860
KOiK00807
TreeFamiTF105328

Family and domain databases

Gene3Di1.20.120.550, 1 hit
InterProiView protein in InterPro
IPR001446 5_LipOase_AP
IPR018295 FLAP/GST2/LTC4S_CS
IPR023352 MAPEG-like_dom_sf
IPR001129 Membr-assoc_MAPEG
PfamiView protein in Pfam
PF01124 MAPEG, 1 hit
PRINTSiPR00488 5LPOXGNASEAP
SUPFAMiSSF161084 SSF161084, 1 hit
PROSITEiView protein in PROSITE
PS01297 FLAP_GST2_LTC4S, 1 hit

Sequencei

Sequence statusi: Complete.

Q60860-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDEVALLAT VTLVGVLLQA YFSLQVISAR RAFHVSPPLT SGPPEFERVF
60 70 80 90 100
RAQVNCSEYF PLFLATLWVA GIFFHEGAAA LCGLFYLFAR LRYFQGYARS
110 120 130 140 150
AQLRLTPLYA SARALWLLVA MAALGLLVHF LPGTLRTALF RWLQMLLPMA
Length:150
Mass (Da):16,814
Last modified:November 1, 1996 - v1
Checksum:i120ABC53892189C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27195 mRNA Translation: AAA75042.1
AL627187 Genomic DNA No translation available.
CCDSiCCDS24631.1
PIRiS68961
RefSeqiNP_032547.1, NM_008521.2
UniGeneiMm.245151

Genome annotation databases

EnsembliENSMUST00000102772; ENSMUSP00000099833; ENSMUSG00000020377
GeneIDi17001
KEGGimmu:17001
UCSCiuc007isa.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLTC4S_MOUSE
AccessioniPrimary (citable) accession number: Q60860
Secondary accession number(s): Q5SVR7, Q9QVS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health