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Protein

Leukotriene C4 synthase

Gene

Ltc4s

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.

Catalytic activityi

Leukotriene C4 = leukotriene A4 + glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30GlutathioneBy similarity1
Active sitei31Proton donorBy similarity1
Active sitei104Proton acceptorBy similarity1

GO - Molecular functioni

  • enzyme activator activity Source: InterPro
  • glutathione peroxidase activity Source: GO_Central
  • glutathione transferase activity Source: GO_Central
  • leukotriene-C4 synthase activity Source: MGI
  • lipid binding Source: MGI

GO - Biological processi

  • leukotriene biosynthetic process Source: GO_Central
  • leukotriene metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Leukotriene biosynthesis

Enzyme and pathway databases

BRENDAi4.4.1.20. 3474.
ReactomeiR-MMU-2142688. Synthesis of 5-eicosatetraenoic acids.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-2142700. Synthesis of Lipoxins (LX).

Chemistry databases

SwissLipidsiSLP:000001453.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukotriene C4 synthase (EC:4.4.1.20)
Short name:
LTC4 synthase
Alternative name(s):
Leukotriene-C(4) synthase
Gene namesi
Name:Ltc4s
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107498. Ltc4s.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicBy similarity6
Transmembranei7 – 27HelicalBy similarityAdd BLAST21
Topological domaini28 – 48LumenalBy similarityAdd BLAST21
Transmembranei49 – 69HelicalBy similarityAdd BLAST21
Topological domaini70 – 73CytoplasmicBy similarity4
Transmembranei74 – 94HelicalBy similarityAdd BLAST21
Topological domaini95 – 104LumenalBy similarity10
Transmembranei105 – 124HelicalBy similarityAdd BLAST20
Topological domaini125 – 150CytoplasmicBy similarityAdd BLAST26

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177491 – 150Leukotriene C4 synthaseAdd BLAST150

Proteomic databases

PaxDbiQ60860.
PeptideAtlasiQ60860.
PRIDEiQ60860.

PTM databases

iPTMnetiQ60860.
PhosphoSitePlusiQ60860.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000020377.
CleanExiMM_LTC4S.
ExpressionAtlasiQ60860. baseline and differential.
GenevisibleiQ60860. MM.

Interactioni

Subunit structurei

Homotrimer. Interacts with ALOX5AP and ALOX5 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099833.

Structurei

Secondary structure

1150
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 5Combined sources4
Helixi6 – 32Combined sources27
Helixi44 – 73Combined sources30
Helixi76 – 99Combined sources24
Helixi102 – 104Combined sources3
Helixi105 – 141Combined sources37

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NTAX-ray2.70A2-150[»]
4NTBX-ray2.70A2-150[»]
4NTFX-ray2.65A2-150[»]
ProteinModelPortaliQ60860.
SMRiQ60860.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 55Glutathione bindingBy similarity5
Regioni58 – 59Glutathione bindingBy similarity2
Regioni93 – 97Glutathione bindingBy similarity5

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IX5F. Eukaryota.
ENOG4111JV7. LUCA.
GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG105513.
InParanoidiQ60860.
KOiK00807.
TreeFamiTF105328.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60860-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDEVALLAT VTLVGVLLQA YFSLQVISAR RAFHVSPPLT SGPPEFERVF
60 70 80 90 100
RAQVNCSEYF PLFLATLWVA GIFFHEGAAA LCGLFYLFAR LRYFQGYARS
110 120 130 140 150
AQLRLTPLYA SARALWLLVA MAALGLLVHF LPGTLRTALF RWLQMLLPMA
Length:150
Mass (Da):16,814
Last modified:November 1, 1996 - v1
Checksum:i120ABC53892189C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27195 mRNA. Translation: AAA75042.1.
AL627187 Genomic DNA. Translation: CAI25121.1.
CCDSiCCDS24631.1.
PIRiS68961.
RefSeqiNP_032547.1. NM_008521.2.
UniGeneiMm.245151.

Genome annotation databases

EnsembliENSMUST00000102772; ENSMUSP00000099833; ENSMUSG00000020377.
GeneIDi17001.
KEGGimmu:17001.
UCSCiuc007isa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27195 mRNA. Translation: AAA75042.1.
AL627187 Genomic DNA. Translation: CAI25121.1.
CCDSiCCDS24631.1.
PIRiS68961.
RefSeqiNP_032547.1. NM_008521.2.
UniGeneiMm.245151.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NTAX-ray2.70A2-150[»]
4NTBX-ray2.70A2-150[»]
4NTFX-ray2.65A2-150[»]
ProteinModelPortaliQ60860.
SMRiQ60860.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099833.

Chemistry databases

SwissLipidsiSLP:000001453.

PTM databases

iPTMnetiQ60860.
PhosphoSitePlusiQ60860.

Proteomic databases

PaxDbiQ60860.
PeptideAtlasiQ60860.
PRIDEiQ60860.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102772; ENSMUSP00000099833; ENSMUSG00000020377.
GeneIDi17001.
KEGGimmu:17001.
UCSCiuc007isa.1. mouse.

Organism-specific databases

CTDi4056.
MGIiMGI:107498. Ltc4s.

Phylogenomic databases

eggNOGiENOG410IX5F. Eukaryota.
ENOG4111JV7. LUCA.
GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG105513.
InParanoidiQ60860.
KOiK00807.
TreeFamiTF105328.

Enzyme and pathway databases

BRENDAi4.4.1.20. 3474.
ReactomeiR-MMU-2142688. Synthesis of 5-eicosatetraenoic acids.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-2142700. Synthesis of Lipoxins (LX).

Miscellaneous databases

PROiQ60860.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020377.
CleanExiMM_LTC4S.
ExpressionAtlasiQ60860. baseline and differential.
GenevisibleiQ60860. MM.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLTC4S_MOUSE
AccessioniPrimary (citable) accession number: Q60860
Secondary accession number(s): Q5SVR7, Q9QVS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.