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Q60860

- LTC4S_MOUSE

UniProt

Q60860 - LTC4S_MOUSE

Protein

Leukotriene C4 synthase

Gene

Ltc4s

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.

    Catalytic activityi

    Leukotriene C4 = leukotriene A4 + glutathione.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei30 – 301GlutathioneBy similarity
    Active sitei31 – 311Proton donorBy similarity
    Active sitei104 – 1041Proton acceptorBy similarity

    GO - Molecular functioni

    1. enzyme activator activity Source: InterPro
    2. glutathione peroxidase activity Source: RefGenome
    3. glutathione transferase activity Source: RefGenome
    4. leukotriene-C4 synthase activity Source: MGI
    5. lipid binding Source: MGI

    GO - Biological processi

    1. leukotriene biosynthetic process Source: RefGenome
    2. leukotriene metabolic process Source: MGI
    3. oxidation-reduction process Source: GOC

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Leukotriene biosynthesis

    Enzyme and pathway databases

    BRENDAi4.4.1.20. 3474.
    ReactomeiREACT_196544. Synthesis of Lipoxins (LX).
    REACT_206751. Synthesis of 5-eicosatetraenoic acids.
    REACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leukotriene C4 synthase (EC:4.4.1.20)
    Short name:
    LTC4 synthase
    Alternative name(s):
    Leukotriene-C(4) synthase
    Gene namesi
    Name:Ltc4s
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:107498. Ltc4s.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: RefGenome
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. nuclear envelope Source: UniProtKB
    5. nuclear outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 150150Leukotriene C4 synthasePRO_0000217749Add
    BLAST

    Proteomic databases

    PRIDEiQ60860.

    PTM databases

    PhosphoSiteiQ60860.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ60860.
    BgeeiQ60860.
    CleanExiMM_LTC4S.
    GenevestigatoriQ60860.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with ALOX5AP and ALOX5 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ60860.
    SMRiQ60860. Positions 1-148.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicBy similarity
    Topological domaini28 – 4821LumenalBy similarityAdd
    BLAST
    Topological domaini70 – 734CytoplasmicBy similarity
    Topological domaini95 – 10410LumenalBy similarity
    Topological domaini125 – 15026CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721HelicalBy similarityAdd
    BLAST
    Transmembranei49 – 6921HelicalBy similarityAdd
    BLAST
    Transmembranei74 – 9421HelicalBy similarityAdd
    BLAST
    Transmembranei105 – 12420HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni51 – 555Glutathione bindingBy similarity
    Regioni58 – 592Glutathione bindingBy similarity
    Regioni93 – 975Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the MAPEG family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00430000030964.
    HOGENOMiHOG000116372.
    HOVERGENiHBG105513.
    InParanoidiQ5SVR7.
    KOiK00807.
    TreeFamiTF105328.

    Family and domain databases

    Gene3Di1.20.120.550. 1 hit.
    InterProiIPR001446. 5_LipOase_AP.
    IPR018295. FLAP/GST2/LTC4S_CS.
    IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view]
    PfamiPF01124. MAPEG. 1 hit.
    [Graphical view]
    PRINTSiPR00488. 5LPOXGNASEAP.
    PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60860-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDEVALLAT VTLVGVLLQA YFSLQVISAR RAFHVSPPLT SGPPEFERVF    50
    RAQVNCSEYF PLFLATLWVA GIFFHEGAAA LCGLFYLFAR LRYFQGYARS 100
    AQLRLTPLYA SARALWLLVA MAALGLLVHF LPGTLRTALF RWLQMLLPMA 150
    Length:150
    Mass (Da):16,814
    Last modified:November 1, 1996 - v1
    Checksum:i120ABC53892189C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27195 mRNA. Translation: AAA75042.1.
    AL627187 Genomic DNA. Translation: CAI25121.1.
    CCDSiCCDS24631.1.
    PIRiS68961.
    RefSeqiNP_032547.1. NM_008521.1.
    UniGeneiMm.245151.

    Genome annotation databases

    EnsembliENSMUST00000102772; ENSMUSP00000099833; ENSMUSG00000020377.
    GeneIDi17001.
    KEGGimmu:17001.
    UCSCiuc007isa.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27195 mRNA. Translation: AAA75042.1 .
    AL627187 Genomic DNA. Translation: CAI25121.1 .
    CCDSi CCDS24631.1.
    PIRi S68961.
    RefSeqi NP_032547.1. NM_008521.1.
    UniGenei Mm.245151.

    3D structure databases

    ProteinModelPortali Q60860.
    SMRi Q60860. Positions 1-148.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q60860.

    Proteomic databases

    PRIDEi Q60860.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102772 ; ENSMUSP00000099833 ; ENSMUSG00000020377 .
    GeneIDi 17001.
    KEGGi mmu:17001.
    UCSCi uc007isa.1. mouse.

    Organism-specific databases

    CTDi 4056.
    MGIi MGI:107498. Ltc4s.

    Phylogenomic databases

    GeneTreei ENSGT00430000030964.
    HOGENOMi HOG000116372.
    HOVERGENi HBG105513.
    InParanoidi Q5SVR7.
    KOi K00807.
    TreeFami TF105328.

    Enzyme and pathway databases

    BRENDAi 4.4.1.20. 3474.
    Reactomei REACT_196544. Synthesis of Lipoxins (LX).
    REACT_206751. Synthesis of 5-eicosatetraenoic acids.
    REACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).

    Miscellaneous databases

    NextBioi 291112.
    PROi Q60860.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q60860.
    Bgeei Q60860.
    CleanExi MM_LTC4S.
    Genevestigatori Q60860.

    Family and domain databases

    Gene3Di 1.20.120.550. 1 hit.
    InterProi IPR001446. 5_LipOase_AP.
    IPR018295. FLAP/GST2/LTC4S_CS.
    IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view ]
    Pfami PF01124. MAPEG. 1 hit.
    [Graphical view ]
    PRINTSi PR00488. 5LPOXGNASEAP.
    PROSITEi PS01297. FLAP_GST2_LTC4S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the gene for mouse leukotriene-C4 synthase."
      Penrose J.F., Baldasaro M.H., Webster M., Xu K., Austen K.F., Lam B.K.
      Eur. J. Biochem. 248:807-813(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Two step purification of human and murine leukotriene C4 synthase."
      Goppelt-Struebe M.
      Biochim. Biophys. Acta 1256:257-261(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-27.
      Tissue: Mast cell.
    4. "Molecular cloning, expression and characterization of mouse leukotriene C4 synthase."
      Lam B.K., Penrose J.F., Rokach J., Xu K., Baldasaro M.H., Austen K.F.
      Eur. J. Biochem. 238:606-612(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiLTC4S_MOUSE
    AccessioniPrimary (citable) accession number: Q60860
    Secondary accession number(s): Q5SVR7, Q9QVS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3