Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leukotriene C4 synthase

Gene

Ltc4s

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.

Catalytic activityi

Leukotriene C4 = leukotriene A4 + glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301GlutathioneBy similarity
Active sitei31 – 311Proton donorBy similarity
Active sitei104 – 1041Proton acceptorBy similarity

GO - Molecular functioni

  • enzyme activator activity Source: InterPro
  • glutathione binding Source: Ensembl
  • glutathione peroxidase activity Source: GO_Central
  • glutathione transferase activity Source: GO_Central
  • leukotriene-C4 synthase activity Source: MGI
  • lipid binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Leukotriene biosynthesis

Enzyme and pathway databases

BRENDAi4.4.1.20. 3474.
ReactomeiREACT_280918. Synthesis of Lipoxins (LX).
REACT_321024. Synthesis of Leukotrienes (LT) and Eoxins (EX).
REACT_327774. Synthesis of 5-eicosatetraenoic acids.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukotriene C4 synthase (EC:4.4.1.20)
Short name:
LTC4 synthase
Alternative name(s):
Leukotriene-C(4) synthase
Gene namesi
Name:Ltc4s
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107498. Ltc4s.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicBy similarity
Transmembranei7 – 2721HelicalBy similarityAdd
BLAST
Topological domaini28 – 4821LumenalBy similarityAdd
BLAST
Transmembranei49 – 6921HelicalBy similarityAdd
BLAST
Topological domaini70 – 734CytoplasmicBy similarity
Transmembranei74 – 9421HelicalBy similarityAdd
BLAST
Topological domaini95 – 10410LumenalBy similarity
Transmembranei105 – 12420HelicalBy similarityAdd
BLAST
Topological domaini125 – 15026CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Leukotriene C4 synthasePRO_0000217749Add
BLAST

Proteomic databases

PRIDEiQ60860.

PTM databases

PhosphoSiteiQ60860.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ60860.
CleanExiMM_LTC4S.
ExpressionAtlasiQ60860. baseline and differential.
GenevisibleiQ60860. MM.

Interactioni

Subunit structurei

Homotrimer. Interacts with ALOX5AP and ALOX5 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099833.

Structurei

Secondary structure

1
150
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 54Combined sources
Helixi6 – 3227Combined sources
Helixi44 – 7330Combined sources
Helixi76 – 9924Combined sources
Helixi102 – 1043Combined sources
Helixi105 – 14137Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4NTAX-ray2.70A2-150[»]
4NTBX-ray2.70A2-150[»]
4NTFX-ray2.65A2-150[»]
ProteinModelPortaliQ60860.
SMRiQ60860. Positions 2-145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 555Glutathione bindingBy similarity
Regioni58 – 592Glutathione bindingBy similarity
Regioni93 – 975Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG105513.
InParanoidiQ60860.
KOiK00807.
TreeFamiTF105328.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60860-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDEVALLAT VTLVGVLLQA YFSLQVISAR RAFHVSPPLT SGPPEFERVF
60 70 80 90 100
RAQVNCSEYF PLFLATLWVA GIFFHEGAAA LCGLFYLFAR LRYFQGYARS
110 120 130 140 150
AQLRLTPLYA SARALWLLVA MAALGLLVHF LPGTLRTALF RWLQMLLPMA
Length:150
Mass (Da):16,814
Last modified:November 1, 1996 - v1
Checksum:i120ABC53892189C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27195 mRNA. Translation: AAA75042.1.
AL627187 Genomic DNA. Translation: CAI25121.1.
CCDSiCCDS24631.1.
PIRiS68961.
RefSeqiNP_032547.1. NM_008521.1.
UniGeneiMm.245151.

Genome annotation databases

EnsembliENSMUST00000102772; ENSMUSP00000099833; ENSMUSG00000020377.
GeneIDi17001.
KEGGimmu:17001.
UCSCiuc007isa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27195 mRNA. Translation: AAA75042.1.
AL627187 Genomic DNA. Translation: CAI25121.1.
CCDSiCCDS24631.1.
PIRiS68961.
RefSeqiNP_032547.1. NM_008521.1.
UniGeneiMm.245151.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4NTAX-ray2.70A2-150[»]
4NTBX-ray2.70A2-150[»]
4NTFX-ray2.65A2-150[»]
ProteinModelPortaliQ60860.
SMRiQ60860. Positions 2-145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099833.

PTM databases

PhosphoSiteiQ60860.

Proteomic databases

PRIDEiQ60860.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102772; ENSMUSP00000099833; ENSMUSG00000020377.
GeneIDi17001.
KEGGimmu:17001.
UCSCiuc007isa.1. mouse.

Organism-specific databases

CTDi4056.
MGIiMGI:107498. Ltc4s.

Phylogenomic databases

GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG105513.
InParanoidiQ60860.
KOiK00807.
TreeFamiTF105328.

Enzyme and pathway databases

BRENDAi4.4.1.20. 3474.
ReactomeiREACT_280918. Synthesis of Lipoxins (LX).
REACT_321024. Synthesis of Leukotrienes (LT) and Eoxins (EX).
REACT_327774. Synthesis of 5-eicosatetraenoic acids.

Miscellaneous databases

NextBioi291112.
PROiQ60860.
SOURCEiSearch...

Gene expression databases

BgeeiQ60860.
CleanExiMM_LTC4S.
ExpressionAtlasiQ60860. baseline and differential.
GenevisibleiQ60860. MM.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the gene for mouse leukotriene-C4 synthase."
    Penrose J.F., Baldasaro M.H., Webster M., Xu K., Austen K.F., Lam B.K.
    Eur. J. Biochem. 248:807-813(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Two step purification of human and murine leukotriene C4 synthase."
    Goppelt-Struebe M.
    Biochim. Biophys. Acta 1256:257-261(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-27.
    Tissue: Mast cell.
  4. "Molecular cloning, expression and characterization of mouse leukotriene C4 synthase."
    Lam B.K., Penrose J.F., Rokach J., Xu K., Baldasaro M.H., Austen K.F.
    Eur. J. Biochem. 238:606-612(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLTC4S_MOUSE
AccessioniPrimary (citable) accession number: Q60860
Secondary accession number(s): Q5SVR7, Q9QVS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.