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Protein

Inactive 2'-5'-oligoadenylate synthase 1B

Gene

Oas1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. The full-length protein displays antiviral activity against flaviviruses such as west Nile virus (WNV) via an alternative antiviral pathway independent of RNase L. The truncated form of the protein lacks antiviral activity.2 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • double-stranded RNA binding Source: UniProtKB
  • transferase activity Source: InterPro

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • negative regulation of viral genome replication Source: UniProtKB
  • response to virus Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive 2'-5'-oligoadenylate synthase 1B
Short name:
(2-5')oligo(A) synthase 1B
Short name:
2-5A synthase 1B
Alternative name(s):
2'-5'-oligoadenylate synthase-like protein 1
Gene namesi
Name:Oas1b
Synonyms:Flv, Oias2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97430. Oas1b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 351351CytoplasmicSequence analysisAdd
BLAST
Transmembranei352 – 37019Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini371 – 3766ExtracellularSequence analysis

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Inactive 2'-5'-oligoadenylate synthase 1BPRO_0000160261Add
BLAST

Proteomic databases

PaxDbiQ60856.
PeptideAtlasiQ60856.
PRIDEiQ60856.

Expressioni

Tissue specificityi

Thymus and spleen.1 Publication

Inductioni

By type I interferon (IFN) and viruses.

Gene expression databases

CleanExiMM_OAS1B.

Interactioni

Subunit structurei

Interacts with OSBPL1A and ABCF3.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083564.

Structurei

3D structure databases

ProteinModelPortaliQ60856.
SMRiQ60856. Positions 4-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 2-5A synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000022614.
HOVERGENiHBG000994.
InParanoidiQ60856.
KOiK14216.

Family and domain databases

Gene3Di1.10.1410.20. 1 hit.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 1 hit.
PfamiPF10421. OAS1_C. 1 hit.
[Graphical view]
PROSITEiPS00833. 25A_SYNTH_2. 1 hit.
PS50152. 25A_SYNTH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60856-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQDLRSIPA SKLDKFIENH LPDTSFCADL REVIDALCAL LKDRSFRGPV
60 70 80 90 100
RRMRASKGVK GKGTTLKGRS DADLVVFLNN LTSFEDQLNQ QGVLIKEIKK
110 120 130 140 150
QLCEVQHERR CGVKFEVHSL RSPNSRALSF KLSAPDLLKE VKFDVLPAYD
160 170 180 190 200
LLDHLNILKK PNQQFYANLI SGRTPPGKEG KLSICFMGLR KYFLNCRPTK
210 220 230 240 250
LKRLIRLVTH WYQLCKEKLG DPLPPQYALE LLTVYAWEYG SRVTKFNTAQ
260 270 280 290 300
GFRTVLELVT KYKQLQIYWT VYYDFRHQEV SEYLHQQLKK DRPVILDPAD
310 320 330 340 350
PTRNIAGLNP KDWRRLAGEA AAWLQYPCFK YRDGSSVCSW EVPTEVGVPM
360 370
KYLLCRIFWL LFWSLFHFIF GKTSSG
Length:376
Mass (Da):43,620
Last modified:September 5, 2012 - v3
Checksum:i0733589FC702D4C7
GO

Sequence cautioni

The sequence BAB84129 differs from that shown. Reason: Frameshift at positions 173, 175 and 177. Curated
The sequence CAA39455 differs from that shown. Reason: Frameshift at positions 173, 175 and 177. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 603GVK → VFQ in CAA39455 (PubMed:1709495).Curated
Sequence conflicti234 – 2352VY → LD in BAB84129 (PubMed:12396720).Curated
Sequence conflicti234 – 2352VY → LD in CAA39455 (PubMed:1709495).Curated

Polymorphismi

A nonsense mutation in exon 4 results in a premature stop codon leading to a truncated form which determines the resistant/susceptible phenotype of the mice to flaviviruses. Mice encoding the full-length protein show a flavivirus resistance phenotype (Flv(r)), whereas mice encoding a C-terminally truncated protein show a flavivirus susceptible phenotype (Flv(s)).1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti36 – 361A → S in strain: BID, DHA, MPR. 1 Publication
Natural varianti45 – 451S → F in strain: MBT/Pas, PWD/Phj. 2 Publications
Natural varianti47 – 471R → Q in strain: DHA. 1 Publication
Natural varianti50 – 501V → G in strain: DHA. 1 Publication
Natural varianti63 – 631G → C in strain: BID, DHA, MPR, MBT/Pas, PWD/Phj. 2 Publications
Natural varianti65 – 651T → A in strain: BALB/c, BID, C57BL/6J, C3H/He, MBT/Pas, PWD/Phj. 7 Publications
Natural varianti83 – 831S → Y in strain: BID, DHA, MPR, MBT/Pas, PWD/Phj. 2 Publications
Natural varianti90 – 901Q → R in strain: DHA. 1 Publication
Natural varianti103 – 1031C → Y in strain: MBT/Pas, PWD/Phj. 2 Publications
Natural varianti105 – 1051V → I in strain: DHA. 1 Publication
Natural varianti111 – 1111C → F in strain: MPR, DHA, MBT/Pas, PWD/Phj. 2 Publications
Natural varianti118 – 1181H → Q in strain: MPR, MBT/Pas, PWD/Phj. 2 Publications
Natural varianti151 – 1511L → V in strain: BID, MPR. 1 Publication
Natural varianti176 – 1761P → L in strain: MBT/Pas, PWD/Phj. 2 Publications
Natural varianti181 – 1811K → E in strain: DHA. 1 Publication
Natural varianti183 – 1831S → L in strain: BID, MBT/Pas, PWD/Phj. 2 Publications
Natural varianti184 – 1841I → T in strain: BID, DHA, MBT/Pas, PWD/Phj. 2 Publications
Natural varianti190 – 1901R → Q in strain: BALB/c, C57BL/6J, C3H/He. 6 Publications
Natural varianti206 – 2061R → H in strain: MBT/Pas, PWD/Phj. 2 Publications
Natural varianti253 – 376124Missing in the truncated form.
Add
BLAST
Natural varianti266 – 2661Q → R in strain: BID, C3H/RV, DHA, MPR, MBT/Pas, PWD/Phj. 3 Publications
Natural varianti277 – 2771H → L in strain: BID. 1 Publication
Natural varianti278 – 2781Q → P in strain: DHA. 1 Publication
Natural varianti291 – 2911D → V in strain: DHA. 1 Publication
Natural varianti299 – 2991A → V in strain: MPR. 1 Publication
Natural varianti305 – 3051I → V in strain: MPR. 1 Publication
Natural varianti322 – 3221A → T in strain: C3H/RV. 1 Publication
Natural varianti336 – 3361S → P in strain: C3H/RV, DHA, MPR, MBT/Pas, PWD/Phj. 3 Publications
Natural varianti347 – 3471G → A in strain: DHA, MBT/Pas, PWD/Phj. 2 Publications
Natural varianti350 – 3501M → T in strain: BID, DHA, MBT/Pas, PWD/Phj. 2 Publications
Natural varianti354 – 3541L → F in strain: BID, C3H/RV, DHA, MPR, MBT/Pas, PWD/Phj. 3 Publications
Natural varianti368 – 3681F → L in strain: MPR. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067529 mRNA. Translation: BAB84129.1. Frameshift.
AF328926 mRNA. Translation: AAM47542.1.
AF418004 mRNA. Translation: AAM47544.1.
AF418005 mRNA. Translation: AAM47545.1.
AF481734 Genomic DNA. Translation: AAM49738.1.
AF466822 mRNA. Translation: AAM97603.1.
AF466823 mRNA. Translation: AAM97604.1.
AM887907 Genomic DNA. Translation: CAP12708.1.
AM887915 Genomic DNA. Translation: CAP12714.1.
AM887917 Genomic DNA. Translation: CAP12716.1.
AM887918 Genomic DNA. Translation: CAP12717.1.
AM887919 Genomic DNA. Translation: CAP12718.1.
AC115937 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19747.1.
X55982 mRNA. Translation: CAA39455.1. Frameshift.
PIRiS15661.
RefSeqiNP_001077394.1. NM_001083925.1.
UniGeneiMm.233471.

Genome annotation databases

GeneIDi23961.
KEGGimmu:23961.
UCSCiuc008zie.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067529 mRNA. Translation: BAB84129.1. Frameshift.
AF328926 mRNA. Translation: AAM47542.1.
AF418004 mRNA. Translation: AAM47544.1.
AF418005 mRNA. Translation: AAM47545.1.
AF481734 Genomic DNA. Translation: AAM49738.1.
AF466822 mRNA. Translation: AAM97603.1.
AF466823 mRNA. Translation: AAM97604.1.
AM887907 Genomic DNA. Translation: CAP12708.1.
AM887915 Genomic DNA. Translation: CAP12714.1.
AM887917 Genomic DNA. Translation: CAP12716.1.
AM887918 Genomic DNA. Translation: CAP12717.1.
AM887919 Genomic DNA. Translation: CAP12718.1.
AC115937 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19747.1.
X55982 mRNA. Translation: CAA39455.1. Frameshift.
PIRiS15661.
RefSeqiNP_001077394.1. NM_001083925.1.
UniGeneiMm.233471.

3D structure databases

ProteinModelPortaliQ60856.
SMRiQ60856. Positions 4-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083564.

Proteomic databases

PaxDbiQ60856.
PeptideAtlasiQ60856.
PRIDEiQ60856.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23961.
KEGGimmu:23961.
UCSCiuc008zie.1. mouse.

Organism-specific databases

CTDi23961.
MGIiMGI:97430. Oas1b.

Phylogenomic databases

HOGENOMiHOG000022614.
HOVERGENiHBG000994.
InParanoidiQ60856.
KOiK14216.

Miscellaneous databases

PROiQ60856.
SOURCEiSearch...

Gene expression databases

CleanExiMM_OAS1B.

Family and domain databases

Gene3Di1.10.1410.20. 1 hit.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 1 hit.
PfamiPF10421. OAS1_C. 1 hit.
[Graphical view]
PROSITEiPS00833. 25A_SYNTH_2. 1 hit.
PS50152. 25A_SYNTH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOAS1B_MOUSE
AccessioniPrimary (citable) accession number: Q60856
Secondary accession number(s): A8YPE5
, A8YPE7, A8YPE8, A8YPE9, E9QKP6, Q78ZX0, Q8JZN0, Q8JZN4, Q8VI98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: September 5, 2012
Last modified: July 6, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.