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Protein

Serpin B6

Gene

Serpinb6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of cathepsin G, kallikrein-8 and thrombin. May play an important role in the inner ear in the protection against leakage of lysosomal content during stress. May be involved in the regulation of serine proteinases present in the brain or extravasated from the blood.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei343 – 3442Reactive bondBy similarity

GO - Molecular functioni

  • protease binding Source: MGI
  • serine-type endopeptidase inhibitor activity Source: BHF-UCL

GO - Biological processi

  • cellular response to osmotic stress Source: MGI
  • gonad development Source: BHF-UCL
  • negative regulation of endopeptidase activity Source: BHF-UCL
  • sensory perception of sound Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.030.

Names & Taxonomyi

Protein namesi
Recommended name:
Serpin B6
Alternative name(s):
Placental thrombin inhibitor
Proteinase inhibitor 6
Short name:
PI-6
Gene namesi
Name:Serpinb6
Synonyms:Serpinb6a, Spi3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:103123. Serpinb6a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular space Source: GO_Central
  • intracellular Source: BHF-UCL
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Serpin B6PRO_0000094107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei197 – 1971N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ60854.
PaxDbiQ60854.
PRIDEiQ60854.

PTM databases

PhosphoSiteiQ60854.

Expressioni

Tissue specificityi

Expressed in the inner ear hair cells, keratinocytes of hair follicles and epidermis in abdominal skin.2 Publications

Developmental stagei

At E13.5, weakly detected in utricle sensory epithelium but not in hair cells. At E16.5, more prominently detected in crista hair cells. Hair cell expression is sustained in postnatal mice. In cochlea, detected in cochlear hair cells in embryo and in hair cells and the greater epithelial ridge (GER) region in early postnatal age.1 Publication

Gene expression databases

BgeeiQ60854.
ExpressionAtlasiQ60854. baseline and differential.
GenevisibleiQ60854. MM.

Interactioni

Subunit structurei

Forms a complex with the monomeric form of beta-tryptase.By similarity

Protein-protein interaction databases

BioGridi203444. 1 interaction.
IntActiQ60854. 2 interactions.
MINTiMINT-1855884.
STRINGi10090.ENSMUSP00000017188.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M37model-A1-378[»]
ProteinModelPortaliQ60854.
SMRiQ60854. Positions 1-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family. Ov-serpin subfamily.Curated

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiQ60854.
KOiK13963.
OrthoDBiEOG7327PB.
PhylomeDBiQ60854.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60854-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPLQEANGT FALNLLKILG EDSSKNVFLS PMSISSALAM VFMGAKGTTA
60 70 80 90 100
SQMAQALALD KCSGNGGGDV HQGFQSLLTE VNKTGTQYLL RTANRLFGDK
110 120 130 140 150
TCDLLASFKD SCLKFYEAEL EELDFQGATE ESRQHINTWV AKKTEDKIKE
160 170 180 190 200
VLSPGTVNSD TSLVLVNAIY FKGNWEKQFN KEHTREMPFK VSKNEEKPVQ
210 220 230 240 250
MMFKKSTFKM TYIGEIFTKI LLLPYVSSEL NMIIMLPDEH VELSTVEKEV
260 270 280 290 300
TYEKFIEWTR LDKMDEEEVE VFLPKFKLEE NYNMNDALYK LGMTDAFGGR
310 320 330 340 350
ADFSGMSSKQ GLFLSKVVHK AFVEVNEEGT EAAAATAGMM TVRCMRFTPR
360 370
FCADHPFLFF IHHVKTNGIL FCGRFSSP
Length:378
Mass (Da):42,599
Last modified:November 1, 1996 - v1
Checksum:i4B0F5E1A030BBDF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25844 mRNA. Translation: AAA79684.1.
BC006766 mRNA. Translation: AAH06766.1.
BC057950 mRNA. Translation: AAH57950.1.
CCDSiCCDS26441.1.
PIRiA57488.
RefSeqiNP_001157590.1. NM_001164118.1.
NP_001230121.1. NM_001243192.1.
NP_033280.1. NM_009254.3.
XP_006516686.1. XM_006516623.1.
UniGeneiMm.252210.
Mm.374799.
Mm.486427.

Genome annotation databases

EnsembliENSMUST00000043552; ENSMUSP00000041016; ENSMUSG00000060147.
ENSMUST00000076532; ENSMUSP00000075848; ENSMUSG00000060147.
ENSMUST00000167163; ENSMUSP00000131115; ENSMUSG00000060147.
GeneIDi20719.
KEGGimmu:20719.
UCSCiuc007qap.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25844 mRNA. Translation: AAA79684.1.
BC006766 mRNA. Translation: AAH06766.1.
BC057950 mRNA. Translation: AAH57950.1.
CCDSiCCDS26441.1.
PIRiA57488.
RefSeqiNP_001157590.1. NM_001164118.1.
NP_001230121.1. NM_001243192.1.
NP_033280.1. NM_009254.3.
XP_006516686.1. XM_006516623.1.
UniGeneiMm.252210.
Mm.374799.
Mm.486427.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M37model-A1-378[»]
ProteinModelPortaliQ60854.
SMRiQ60854. Positions 1-378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203444. 1 interaction.
IntActiQ60854. 2 interactions.
MINTiMINT-1855884.
STRINGi10090.ENSMUSP00000017188.

Protein family/group databases

MEROPSiI04.030.

PTM databases

PhosphoSiteiQ60854.

Proteomic databases

MaxQBiQ60854.
PaxDbiQ60854.
PRIDEiQ60854.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043552; ENSMUSP00000041016; ENSMUSG00000060147.
ENSMUST00000076532; ENSMUSP00000075848; ENSMUSG00000060147.
ENSMUST00000167163; ENSMUSP00000131115; ENSMUSG00000060147.
GeneIDi20719.
KEGGimmu:20719.
UCSCiuc007qap.2. mouse.

Organism-specific databases

CTDi20719.
MGIiMGI:103123. Serpinb6a.

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiQ60854.
KOiK13963.
OrthoDBiEOG7327PB.
PhylomeDBiQ60854.

Miscellaneous databases

NextBioi299313.
PROiQ60854.
SOURCEiSearch...

Gene expression databases

BgeeiQ60854.
ExpressionAtlasiQ60854. baseline and differential.
GenevisibleiQ60854. MM.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene structure, chromosomal localization, and expression of the murine homologue of human proteinase inhibitor 6 (PI-6) suggests divergence of PI-6 from the ovalbumin serpins."
    Sun J., Rose J.B., Bird P.
    J. Biol. Chem. 270:16089-16096(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  3. "SerpinB6 is an inhibitor of kallikrein-8 in keratinocytes."
    Scott F.L., Sun J., Whisstock J.C., Kato K., Bird P.I.
    J. Biochem. 142:435-442(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-197, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSPB6_MOUSE
AccessioniPrimary (citable) accession number: Q60854
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.