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Q60854 (SPB6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serpin B6
Alternative name(s):
Placental thrombin inhibitor
Proteinase inhibitor 6
Short name=PI-6
Gene names
Name:Serpinb6
Synonyms:Serpinb6a, Spi3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of cathepsin G, kallikrein-8 and thrombin. May play an important role in the inner ear in the protection against leakage of lysosomal content during stress. May be involved in the regulation of serine proteinases present in the brain or extravasated from the blood. Ref.3

Subunit structure

Forms a complex with the monomeric form of beta-tryptase By similarity.

Subcellular location

Cytoplasm Ref.3 Ref.4.

Tissue specificity

Expressed in the inner ear hair cells, keratinocytes of hair follicles and epidermis in abdominal skin. Ref.3 Ref.4

Developmental stage

At E13.5, weakly detected in utricle sensory epithelium but not in hair cells. At E16.5, more prominently detected in crista hair cells. Hair cell expression is sustained in postnatal mice. In cochlea, detected in cochlear hair cells in embryo and in hair cells and the greater epithelial ridge (GER) region in early postnatal age. Ref.4

Sequence similarities

Belongs to the serpin family. Ov-serpin subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 378378Serpin B6
PRO_0000094107

Sites

Site343 – 3442Reactive bond By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue1971N6-acetyllysine Ref.5

Secondary structure

.............................................................. 378
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q60854 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4B0F5E1A030BBDF6

FASTA37842,599
        10         20         30         40         50         60 
MDPLQEANGT FALNLLKILG EDSSKNVFLS PMSISSALAM VFMGAKGTTA SQMAQALALD 

        70         80         90        100        110        120 
KCSGNGGGDV HQGFQSLLTE VNKTGTQYLL RTANRLFGDK TCDLLASFKD SCLKFYEAEL 

       130        140        150        160        170        180 
EELDFQGATE ESRQHINTWV AKKTEDKIKE VLSPGTVNSD TSLVLVNAIY FKGNWEKQFN 

       190        200        210        220        230        240 
KEHTREMPFK VSKNEEKPVQ MMFKKSTFKM TYIGEIFTKI LLLPYVSSEL NMIIMLPDEH 

       250        260        270        280        290        300 
VELSTVEKEV TYEKFIEWTR LDKMDEEEVE VFLPKFKLEE NYNMNDALYK LGMTDAFGGR 

       310        320        330        340        350        360 
ADFSGMSSKQ GLFLSKVVHK AFVEVNEEGT EAAAATAGMM TVRCMRFTPR FCADHPFLFF 

       370 
IHHVKTNGIL FCGRFSSP 

« Hide

References

« Hide 'large scale' references
[1]"Gene structure, chromosomal localization, and expression of the murine homologue of human proteinase inhibitor 6 (PI-6) suggests divergence of PI-6 from the ovalbumin serpins."
Sun J., Rose J.B., Bird P.
J. Biol. Chem. 270:16089-16096(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[3]"SerpinB6 is an inhibitor of kallikrein-8 in keratinocytes."
Scott F.L., Sun J., Whisstock J.C., Kato K., Bird P.I.
J. Biochem. 142:435-442(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]"A truncating mutation in SERPINB6 is associated with autosomal-recessive nonsyndromic sensorineural hearing loss."
Sirmaci A., Erbek S., Price J., Huang M., Duman D., Cengiz F.B., Bademci G., Tokgoz-Yilmaz S., Hismi B., Ozdag H., Ozturk B., Kulaksizoglu S., Yildirim E., Kokotas H., Grigoriadou M., Petersen M.B., Shahin H., Kanaan M. expand/collapse author list , King M.C., Chen Z.Y., Blanton S.H., Liu X.Z., Zuchner S., Akar N., Tekin M.
Am. J. Hum. Genet. 86:797-804(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-197, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25844 mRNA. Translation: AAA79684.1.
BC006766 mRNA. Translation: AAH06766.1.
BC057950 mRNA. Translation: AAH57950.1.
CCDSCCDS26441.1.
PIRA57488.
RefSeqNP_001157590.1. NM_001164118.1.
NP_001230121.1. NM_001243192.1.
NP_033280.1. NM_009254.3.
XP_006516686.1. XM_006516623.1.
XP_006516687.1. XM_006516624.1.
UniGeneMm.252210.
Mm.374799.
Mm.486427.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M37model-A1-378[»]
ProteinModelPortalQ60854.
SMRQ60854. Positions 1-378.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ60854. 2 interactions.
MINTMINT-1855884.

Protein family/group databases

MEROPSI04.030.

PTM databases

PhosphoSiteQ60854.

Proteomic databases

MaxQBQ60854.
PaxDbQ60854.
PRIDEQ60854.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043552; ENSMUSP00000041016; ENSMUSG00000060147.
ENSMUST00000076532; ENSMUSP00000075848; ENSMUSG00000060147.
ENSMUST00000167163; ENSMUSP00000131115; ENSMUSG00000060147.
GeneID20719.
KEGGmmu:20719.
UCSCuc007qap.2. mouse.

Organism-specific databases

CTD20719.
MGIMGI:103123. Serpinb6a.

Phylogenomic databases

eggNOGCOG4826.
GeneTreeENSGT00740000114938.
HOGENOMHOG000238519.
HOVERGENHBG005957.
InParanoidQ60854.
KOK13963.
OrthoDBEOG7327PB.
PhylomeDBQ60854.

Gene expression databases

ArrayExpressQ60854.
BgeeQ60854.
GenevestigatorQ60854.

Family and domain databases

InterProIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio299313.
PROQ60854.
SOURCESearch...

Entry information

Entry nameSPB6_MOUSE
AccessionPrimary (citable) accession number: Q60854
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot