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Q60854

- SPB6_MOUSE

UniProt

Q60854 - SPB6_MOUSE

Protein

Serpin B6

Gene

Serpinb6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Inhibitor of cathepsin G, kallikrein-8 and thrombin. May play an important role in the inner ear in the protection against leakage of lysosomal content during stress. May be involved in the regulation of serine proteinases present in the brain or extravasated from the blood.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei343 – 3442Reactive bondBy similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. serine-type endopeptidase inhibitor activity Source: BHF-UCL

    GO - Biological processi

    1. gonad development Source: BHF-UCL
    2. negative regulation of endopeptidase activity Source: BHF-UCL
    3. regulation of proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI04.030.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serpin B6
    Alternative name(s):
    Placental thrombin inhibitor
    Proteinase inhibitor 6
    Short name:
    PI-6
    Gene namesi
    Name:Serpinb6
    Synonyms:Serpinb6a, Spi3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:103123. Serpinb6a.

    Subcellular locationi

    Cytoplasm 2 Publications

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. extracellular space Source: InterPro
    3. intracellular Source: BHF-UCL

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 378378Serpin B6PRO_0000094107Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei197 – 1971N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ60854.
    PaxDbiQ60854.
    PRIDEiQ60854.

    PTM databases

    PhosphoSiteiQ60854.

    Expressioni

    Tissue specificityi

    Expressed in the inner ear hair cells, keratinocytes of hair follicles and epidermis in abdominal skin.2 Publications

    Developmental stagei

    At E13.5, weakly detected in utricle sensory epithelium but not in hair cells. At E16.5, more prominently detected in crista hair cells. Hair cell expression is sustained in postnatal mice. In cochlea, detected in cochlear hair cells in embryo and in hair cells and the greater epithelial ridge (GER) region in early postnatal age.1 Publication

    Gene expression databases

    ArrayExpressiQ60854.
    BgeeiQ60854.
    GenevestigatoriQ60854.

    Interactioni

    Subunit structurei

    Forms a complex with the monomeric form of beta-tryptase.By similarity

    Protein-protein interaction databases

    IntActiQ60854. 2 interactions.
    MINTiMINT-1855884.

    Structurei

    Secondary structure

    1
    378
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 2120
    Helixi31 – 4414
    Helixi47 – 5711
    Helixi59 – 613
    Helixi65 – 7713
    Beta strandi82 – 9716
    Helixi101 – 11111
    Beta strandi114 – 1185
    Turni120 – 1223
    Helixi124 – 13916
    Beta strandi157 – 17216
    Helixi176 – 1783
    Beta strandi180 – 18910
    Beta strandi191 – 20818
    Turni209 – 2124
    Beta strandi213 – 23422
    Beta strandi236 – 2383
    Helixi241 – 2444
    Helixi247 – 2548
    Helixi256 – 2583
    Beta strandi260 – 26910
    Beta strandi271 – 2733
    Beta strandi276 – 2783
    Helixi281 – 2844
    Turni285 – 2873
    Turni290 – 2923
    Turni294 – 2963
    Turni300 – 3023
    Beta strandi312 – 3209
    Beta strandi322 – 33615
    Beta strandi350 – 3545
    Turni355 – 3584
    Beta strandi359 – 3635

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M37model-A1-378[»]
    ProteinModelPortaliQ60854.
    SMRiQ60854. Positions 1-378.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the serpin family. Ov-serpin subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG4826.
    GeneTreeiENSGT00740000114938.
    HOGENOMiHOG000238519.
    HOVERGENiHBG005957.
    InParanoidiQ60854.
    KOiK13963.
    OrthoDBiEOG7327PB.
    PhylomeDBiQ60854.

    Family and domain databases

    InterProiIPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view]
    PANTHERiPTHR11461. PTHR11461. 1 hit.
    PfamiPF00079. Serpin. 1 hit.
    [Graphical view]
    SMARTiSM00093. SERPIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF56574. SSF56574. 1 hit.
    PROSITEiPS00284. SERPIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60854-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPLQEANGT FALNLLKILG EDSSKNVFLS PMSISSALAM VFMGAKGTTA    50
    SQMAQALALD KCSGNGGGDV HQGFQSLLTE VNKTGTQYLL RTANRLFGDK 100
    TCDLLASFKD SCLKFYEAEL EELDFQGATE ESRQHINTWV AKKTEDKIKE 150
    VLSPGTVNSD TSLVLVNAIY FKGNWEKQFN KEHTREMPFK VSKNEEKPVQ 200
    MMFKKSTFKM TYIGEIFTKI LLLPYVSSEL NMIIMLPDEH VELSTVEKEV 250
    TYEKFIEWTR LDKMDEEEVE VFLPKFKLEE NYNMNDALYK LGMTDAFGGR 300
    ADFSGMSSKQ GLFLSKVVHK AFVEVNEEGT EAAAATAGMM TVRCMRFTPR 350
    FCADHPFLFF IHHVKTNGIL FCGRFSSP 378
    Length:378
    Mass (Da):42,599
    Last modified:November 1, 1996 - v1
    Checksum:i4B0F5E1A030BBDF6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25844 mRNA. Translation: AAA79684.1.
    BC006766 mRNA. Translation: AAH06766.1.
    BC057950 mRNA. Translation: AAH57950.1.
    CCDSiCCDS26441.1.
    PIRiA57488.
    RefSeqiNP_001157590.1. NM_001164118.1.
    NP_001230121.1. NM_001243192.1.
    NP_033280.1. NM_009254.3.
    XP_006516686.1. XM_006516623.1.
    XP_006516687.1. XM_006516624.1.
    UniGeneiMm.252210.
    Mm.374799.
    Mm.486427.

    Genome annotation databases

    EnsembliENSMUST00000043552; ENSMUSP00000041016; ENSMUSG00000060147.
    ENSMUST00000076532; ENSMUSP00000075848; ENSMUSG00000060147.
    ENSMUST00000167163; ENSMUSP00000131115; ENSMUSG00000060147.
    GeneIDi20719.
    KEGGimmu:20719.
    UCSCiuc007qap.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25844 mRNA. Translation: AAA79684.1 .
    BC006766 mRNA. Translation: AAH06766.1 .
    BC057950 mRNA. Translation: AAH57950.1 .
    CCDSi CCDS26441.1.
    PIRi A57488.
    RefSeqi NP_001157590.1. NM_001164118.1.
    NP_001230121.1. NM_001243192.1.
    NP_033280.1. NM_009254.3.
    XP_006516686.1. XM_006516623.1.
    XP_006516687.1. XM_006516624.1.
    UniGenei Mm.252210.
    Mm.374799.
    Mm.486427.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M37 model - A 1-378 [» ]
    ProteinModelPortali Q60854.
    SMRi Q60854. Positions 1-378.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q60854. 2 interactions.
    MINTi MINT-1855884.

    Protein family/group databases

    MEROPSi I04.030.

    PTM databases

    PhosphoSitei Q60854.

    Proteomic databases

    MaxQBi Q60854.
    PaxDbi Q60854.
    PRIDEi Q60854.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043552 ; ENSMUSP00000041016 ; ENSMUSG00000060147 .
    ENSMUST00000076532 ; ENSMUSP00000075848 ; ENSMUSG00000060147 .
    ENSMUST00000167163 ; ENSMUSP00000131115 ; ENSMUSG00000060147 .
    GeneIDi 20719.
    KEGGi mmu:20719.
    UCSCi uc007qap.2. mouse.

    Organism-specific databases

    CTDi 20719.
    MGIi MGI:103123. Serpinb6a.

    Phylogenomic databases

    eggNOGi COG4826.
    GeneTreei ENSGT00740000114938.
    HOGENOMi HOG000238519.
    HOVERGENi HBG005957.
    InParanoidi Q60854.
    KOi K13963.
    OrthoDBi EOG7327PB.
    PhylomeDBi Q60854.

    Miscellaneous databases

    NextBioi 299313.
    PROi Q60854.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q60854.
    Bgeei Q60854.
    Genevestigatori Q60854.

    Family and domain databases

    InterProi IPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view ]
    PANTHERi PTHR11461. PTHR11461. 1 hit.
    Pfami PF00079. Serpin. 1 hit.
    [Graphical view ]
    SMARTi SM00093. SERPIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56574. SSF56574. 1 hit.
    PROSITEi PS00284. SERPIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene structure, chromosomal localization, and expression of the murine homologue of human proteinase inhibitor 6 (PI-6) suggests divergence of PI-6 from the ovalbumin serpins."
      Sun J., Rose J.B., Bird P.
      J. Biol. Chem. 270:16089-16096(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    3. "SerpinB6 is an inhibitor of kallikrein-8 in keratinocytes."
      Scott F.L., Sun J., Whisstock J.C., Kato K., Bird P.I.
      J. Biochem. 142:435-442(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    4. Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-197, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSPB6_MOUSE
    AccessioniPrimary (citable) accession number: Q60854
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3