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Q60848

- HELLS_MOUSE

UniProt

Q60848 - HELLS_MOUSE

Protein

Lymphocyte-specific helicase

Gene

Hells

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.9 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi231 – 2388ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. chromatin binding Source: MGI
    3. DNA binding Source: InterPro
    4. helicase activity Source: UniProtKB-KW
    5. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin silencing Source: MGI
    2. DNA methylation Source: MGI
    3. lymphocyte differentiation Source: MGI
    4. lymphocyte proliferation Source: UniProtKB
    5. maintenance of DNA methylation Source: UniProtKB
    6. methylation-dependent chromatin silencing Source: UniProtKB
    7. mitotic nuclear division Source: UniProtKB-KW
    8. multicellular organismal development Source: UniProtKB
    9. negative regulation of apoptotic process Source: MGI
    10. negative regulation of intrinsic apoptotic signaling pathway Source: MGI
    11. pericentric heterochromatin assembly Source: UniProtKB
    12. transcription, DNA-templated Source: UniProtKB-KW
    13. urogenital system development Source: MGI

    Keywords - Molecular functioni

    Developmental protein, Helicase, Hydrolase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lymphocyte-specific helicase (EC:3.6.4.-)
    Alternative name(s):
    Proliferation-associated SNF2-like protein
    Gene namesi
    Name:HellsImported
    Synonyms:Lsh1 Publication, PasgImported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:106209. Hells.

    Subcellular locationi

    Nucleus 3 Publications
    Note: Closely associated with pericentric heterochromatin.

    GO - Cellular componenti

    1. chromosome, centromeric region Source: UniProtKB
    2. nucleus Source: MGI
    3. pericentric heterochromatin Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 821821Lymphocyte-specific helicasePRO_0000260052Add
    BLAST

    Proteomic databases

    PaxDbiQ60848.
    PRIDEiQ60848.

    PTM databases

    PhosphoSiteiQ60848.

    Expressioni

    Tissue specificityi

    Highly expressed in thymus, bone marrow, and testis. Not detected in heart, brain, liver, kidney, skeletal muscle, spleen, peripheral blood lymphocytes, small intestine, colon, prostate or ovary.3 Publications

    Developmental stagei

    Differentially expressed during embryonic development, with highest expression in developing face, limbs, skeletal muscle, heart, and tail. Highly expressed in fetal thymocytes from day 14 to 16 of gestation, and expressed at much lower levels in adult thymus.3 Publications

    Inductioni

    By concanavalin or lipopolysaccharide in unactivated splenocytes.2 Publications

    Gene expression databases

    ArrayExpressiQ60848.
    BgeeiQ60848.
    CleanExiMM_HELLS.
    GenevestigatoriQ60848.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Dnmt3aO885084EBI-3043887,EBI-995154
    Dnmt3bO885094EBI-3043887,EBI-7987547

    Protein-protein interaction databases

    BioGridi200271. 5 interactions.
    DIPiDIP-43735N.
    IntActiQ60848. 4 interactions.
    MINTiMINT-2521001.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60848.
    SMRiQ60848. Positions 175-739.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini218 – 386169Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini586 – 736151Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili14 – 9683Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi337 – 3404DEAH boxSequence Analysis

    Sequence similaritiesi

    Belongs to the SNF2/RAD54 helicase family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0553.
    GeneTreeiENSGT00740000115593.
    HOGENOMiHOG000172362.
    HOVERGENiHBG060049.
    InParanoidiQ60848.
    OMAiWDRESVD.
    OrthoDBiEOG7TXKG5.
    PhylomeDBiQ60848.
    TreeFamiTF329077.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view]
    PfamiPF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 12 Publications (identifier: Q60848-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEQTEPAVI TPAMLEEEEQ LEAAGLEKER KMLEEAQKSW DRESTEIRYR    50
    RLQHLLEKSN IYSKFLLTKM EQQQLEEQKK KEKLEKKKRS LKLTEGKSLV 100
    DGNGEKPVMK KKRGREDESY NISEVMSKEE ILSVAKKHKD NEDESSSTTS 150
    LCVEDIQKNK DSNSMIKDRL SQTVRQNSKF FFDPVRKCNG QPVPFQQPKH 200
    FTGGVMRWYQ VEGMEWLRML WENGINGILA DEMGLGKTVQ CIATIALMIQ 250
    RGVPGPFLVC GPLSTLPNWM AEFKRFTPEI PTLLYHGTRE DRRKLVKNIH 300
    KRQGTLQIHP VVVTSFEIAM RDQNALQHCY WKYLIVDEGH RIKNMKCRLI 350
    RELKRFNADN KLLLTGTPLQ NNLSELWSLL NFLLPDVFDD LKSFESWFDI 400
    TSLSETAEDI IAKEREQNVL HMLHQILTPF LLRRLKSDVA LEVPPKREVV 450
    VYAPLCNKQE IFYTAIVNRT IANMFGSCEK ETVELSPTGR PKRRSRKSIN 500
    YSELDQFPSE LEKLISQIQP EVNRERTVVE GNIPIESEVN LKLRNIMMLL 550
    RKCCNHPYMI EYPIDPVTQE FKIDEELVTN SGKFLILDRM LPELKKRGHK 600
    VLVFSQMTSM LDILMDYCHL RNFIFSRLDG SMSYSEREKN IYSFNTDPDV 650
    FLFLVSTRAG GLGINLTAAD TVIIYDSDWN PQSDLQAQDR CHRIGQTKPV 700
    VVYRLVTANT IDQKIVERAA AKRKLEKLII HKNHFKGGQS GLSQSKNFLD 750
    AKELMELLKS RDYEREVKGS REKVISDEDL ELLLDRSDLI DQMKASRPIK 800
    GKTGIFKILE NSEDSSAECL F 821
    Length:821
    Mass (Da):95,126
    Last modified:March 1, 2001 - v2
    Checksum:i8DEC2F2EEFBBD2F5
    GO
    Isoform 21 Publication (identifier: Q60848-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-13: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:808
    Mass (Da):93,786
    Checksum:iDBF5E847C1209568
    GO

    Sequence cautioni

    The sequence AAH20056.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti497 – 4971K → R in AAI00395. (PubMed:15489334)Curated
    Sequence conflicti620 – 6201L → P in AAI00395. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1313Missing in isoform 2. 1 PublicationVSP_052235Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25691 mRNA. Translation: AAB08015.2.
    AF155210 mRNA. Translation: AAG43373.1.
    AK013266 mRNA. Translation: BAB28757.2.
    AK147126 mRNA. Translation: BAE27697.1.
    BC020056 mRNA. Translation: AAH20056.1. Different initiation.
    BC100394 mRNA. Translation: AAI00395.1.
    CCDSiCCDS29789.1. [Q60848-1]
    PIRiJC4666.
    RefSeqiNP_032260.2. NM_008234.3. [Q60848-1]
    XP_006526762.1. XM_006526699.1. [Q60848-1]
    UniGeneiMm.392920.
    Mm.486446.
    Mm.57223.

    Genome annotation databases

    EnsembliENSMUST00000025965; ENSMUSP00000025965; ENSMUSG00000025001. [Q60848-1]
    GeneIDi15201.
    KEGGimmu:15201.
    UCSCiuc008hjt.1. mouse. [Q60848-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25691 mRNA. Translation: AAB08015.2 .
    AF155210 mRNA. Translation: AAG43373.1 .
    AK013266 mRNA. Translation: BAB28757.2 .
    AK147126 mRNA. Translation: BAE27697.1 .
    BC020056 mRNA. Translation: AAH20056.1 . Different initiation.
    BC100394 mRNA. Translation: AAI00395.1 .
    CCDSi CCDS29789.1. [Q60848-1 ]
    PIRi JC4666.
    RefSeqi NP_032260.2. NM_008234.3. [Q60848-1 ]
    XP_006526762.1. XM_006526699.1. [Q60848-1 ]
    UniGenei Mm.392920.
    Mm.486446.
    Mm.57223.

    3D structure databases

    ProteinModelPortali Q60848.
    SMRi Q60848. Positions 175-739.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200271. 5 interactions.
    DIPi DIP-43735N.
    IntActi Q60848. 4 interactions.
    MINTi MINT-2521001.

    PTM databases

    PhosphoSitei Q60848.

    Proteomic databases

    PaxDbi Q60848.
    PRIDEi Q60848.

    Protocols and materials databases

    DNASUi 15201.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025965 ; ENSMUSP00000025965 ; ENSMUSG00000025001 . [Q60848-1 ]
    GeneIDi 15201.
    KEGGi mmu:15201.
    UCSCi uc008hjt.1. mouse. [Q60848-1 ]

    Organism-specific databases

    CTDi 3070.
    MGIi MGI:106209. Hells.

    Phylogenomic databases

    eggNOGi COG0553.
    GeneTreei ENSGT00740000115593.
    HOGENOMi HOG000172362.
    HOVERGENi HBG060049.
    InParanoidi Q60848.
    OMAi WDRESVD.
    OrthoDBi EOG7TXKG5.
    PhylomeDBi Q60848.
    TreeFami TF329077.

    Miscellaneous databases

    NextBioi 287739.
    PROi Q60848.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q60848.
    Bgeei Q60848.
    CleanExi MM_HELLS.
    Genevestigatori Q60848.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view ]
    Pfami PF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE, INDUCTION.
      Strain: C57BL/6Imported.
      Tissue: Fetal thymusImported.
    2. "An SNF2 factor involved in mammalian development and cellular proliferation."
      Raabe E.H., Abdurrahman L., Behbehani G., Arececi R.J.
      Dev. Dyn. 221:92-105(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: C57BL/6Imported.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6JImported.
      Tissue: EmbryoImported and HeartImported.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6JImported and FVB/NImported.
      Tissue: EggImported and Mammary glandImported.
    5. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 31-38, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    6. "Characterization of gene expression, genomic structure, and chromosomal localization of Hells (Lsh)."
      Geiman T.M., Durum S.K., Muegge K.
      Genomics 54:477-483(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Lsh, an SNF2/helicase family member, is required for proliferation of mature T lymphocytes."
      Geiman T.M., Muegge K.
      Proc. Natl. Acad. Sci. U.S.A. 97:4772-4777(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
    8. "Lsh, a SNF2 family member, is required for normal murine development."
      Geiman T.M., Tessarollo L., Anver M.R., Kopp J.B., Ward J.M., Muegge K.
      Biochim. Biophys. Acta 1526:211-220(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    9. "Lsh, a member of the SNF2 family, is required for genome-wide methylation."
      Dennis K., Fan T., Geiman T., Yan Q., Muegge K.
      Genes Dev. 15:2940-2944(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Lsh, a modulator of CpG methylation, is crucial for normal histone methylation."
      Yan Q., Huang J., Fan T., Zhu H., Muegge K.
      EMBO J. 22:5154-5162(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    11. "Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin."
      Yan Q., Cho E., Lockett S., Muegge K.
      Mol. Cell. Biol. 23:8416-8428(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    12. "Growth retardation and premature aging phenotypes in mice with disruption of the SNF2-like gene, PASG."
      Sun L.-Q., Lee D.W., Zhang Q., Xiao W., Raabe E.H., Meeker A., Miao D., Huso D.L., Arceci R.J.
      Genes Dev. 18:1035-1046(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Lsh controls silencing of the imprinted Cdkn1c gene."
      Fan T., Hagan J.P., Kozlov S.V., Stewart C.L., Muegge K.
      Development 132:635-644(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiHELLS_MOUSE
    AccessioniPrimary (citable) accession number: Q60848
    Secondary accession number(s): Q497T3, Q8VDZ1, Q9CYV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3