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Q60848 (HELLS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lymphocyte-specific helicase

EC=3.6.4.-
Alternative name(s):
Proliferation-associated SNF2-like protein
Gene names
Name:Hells
Synonyms:Lsh, Pasg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length821 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis. Ref.1 Ref.2 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Subcellular location

Nucleus. Note: Closely associated with pericentric heterochromatin. Ref.7 Ref.10 Ref.11

Tissue specificity

Highly expressed in thymus, bone marrow, and testis. Not detected in heart, brain, liver, kidney, skeletal muscle, spleen, peripheral blood lymphocytes, small intestine, colon, prostate or ovary. Ref.2 Ref.6 Ref.8

Developmental stage

Differentially expressed during embryonic development, with highest expression in developing face, limbs, skeletal muscle, heart, and tail. Highly expressed in fetal thymocytes from day 14 to 16 of gestation, and expressed at much lower levels in adult thymus. Ref.1 Ref.2 Ref.7

Induction

By concanavalin or lipopolysaccharide in unactivated splenocytes. Ref.1 Ref.7

Sequence similarities

Belongs to the SNF2/RAD54 helicase family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence caution

The sequence AAH20056.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Helicase
Hydrolase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processDNA methylation

Inferred from mutant phenotype Ref.9. Source: MGI

centromeric heterochromatin assembly

Inferred from mutant phenotype Ref.11. Source: UniProtKB

chromatin silencing

Inferred from mutant phenotype PubMed 15448183. Source: MGI

lymphocyte differentiation

Traceable author statement Ref.8. Source: MGI

lymphocyte proliferation

Inferred from expression pattern Ref.7. Source: UniProtKB

maintenance of DNA methylation

Inferred from mutant phenotype Ref.12. Source: UniProtKB

methylation-dependent chromatin silencing

Inferred from mutant phenotype Ref.13. Source: UniProtKB

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

multicellular organismal development

Inferred from mutant phenotype Ref.8. Source: UniProtKB

negative regulation of apoptotic process

Inferred from mutant phenotype Ref.8. Source: MGI

negative regulation of intrinsic apoptotic signaling pathway

Inferred from mutant phenotype Ref.8. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

urogenital system development

Inferred from mutant phenotype Ref.8. Source: MGI

   Cellular_componentcentromeric heterochromatin

Inferred from direct assay Ref.11. Source: UniProtKB

chromosome, centromeric region

Inferred from direct assay Ref.11. Source: UniProtKB

nucleus

Inferred from direct assay PubMed 11555636. Source: MGI

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: InterPro

chromatin binding

Inferred from direct assay PubMed 15448183. Source: MGI

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dnmt3aO885084EBI-3043887,EBI-995154
Dnmt3bO885094EBI-3043887,EBI-7987547

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 Ref.2 (identifier: Q60848-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.3 (identifier: Q60848-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 821821Lymphocyte-specific helicase
PRO_0000260052

Regions

Domain218 – 386169Helicase ATP-binding
Domain586 – 736151Helicase C-terminal
Nucleotide binding231 – 2388ATP By similarity
Coiled coil14 – 9683 Potential
Motif337 – 3404DEAH box

Natural variations

Alternative sequence1 – 1313Missing in isoform 2. Ref.3
VSP_052235

Experimental info

Sequence conflict4971K → R in AAI00395. Ref.4
Sequence conflict6201L → P in AAI00395. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 2.
Checksum: 8DEC2F2EEFBBD2F5

FASTA82195,126
        10         20         30         40         50         60 
MAEQTEPAVI TPAMLEEEEQ LEAAGLEKER KMLEEAQKSW DRESTEIRYR RLQHLLEKSN 

        70         80         90        100        110        120 
IYSKFLLTKM EQQQLEEQKK KEKLEKKKRS LKLTEGKSLV DGNGEKPVMK KKRGREDESY 

       130        140        150        160        170        180 
NISEVMSKEE ILSVAKKHKD NEDESSSTTS LCVEDIQKNK DSNSMIKDRL SQTVRQNSKF 

       190        200        210        220        230        240 
FFDPVRKCNG QPVPFQQPKH FTGGVMRWYQ VEGMEWLRML WENGINGILA DEMGLGKTVQ 

       250        260        270        280        290        300 
CIATIALMIQ RGVPGPFLVC GPLSTLPNWM AEFKRFTPEI PTLLYHGTRE DRRKLVKNIH 

       310        320        330        340        350        360 
KRQGTLQIHP VVVTSFEIAM RDQNALQHCY WKYLIVDEGH RIKNMKCRLI RELKRFNADN 

       370        380        390        400        410        420 
KLLLTGTPLQ NNLSELWSLL NFLLPDVFDD LKSFESWFDI TSLSETAEDI IAKEREQNVL 

       430        440        450        460        470        480 
HMLHQILTPF LLRRLKSDVA LEVPPKREVV VYAPLCNKQE IFYTAIVNRT IANMFGSCEK 

       490        500        510        520        530        540 
ETVELSPTGR PKRRSRKSIN YSELDQFPSE LEKLISQIQP EVNRERTVVE GNIPIESEVN 

       550        560        570        580        590        600 
LKLRNIMMLL RKCCNHPYMI EYPIDPVTQE FKIDEELVTN SGKFLILDRM LPELKKRGHK 

       610        620        630        640        650        660 
VLVFSQMTSM LDILMDYCHL RNFIFSRLDG SMSYSEREKN IYSFNTDPDV FLFLVSTRAG 

       670        680        690        700        710        720 
GLGINLTAAD TVIIYDSDWN PQSDLQAQDR CHRIGQTKPV VVYRLVTANT IDQKIVERAA 

       730        740        750        760        770        780 
AKRKLEKLII HKNHFKGGQS GLSQSKNFLD AKELMELLKS RDYEREVKGS REKVISDEDL 

       790        800        810        820 
ELLLDRSDLI DQMKASRPIK GKTGIFKILE NSEDSSAECL F 

« Hide

Isoform 2 [UniParc].

Checksum: DBF5E847C1209568
Show »

FASTA80893,786

References

« Hide 'large scale' references
[1]"A novel putative helicase produced in early murine lymphocytes."
Jarvis C.D., Geiman T., Vila-Storm M.P., Osipovich O., Akella U., Candeias S., Nathan I., Durum S.K., Muegge K.
Gene 169:203-207(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE, INDUCTION.
Strain: C57BL/6.
Tissue: Fetal thymus.
[2]"An SNF2 factor involved in mammalian development and cellular proliferation."
Raabe E.H., Abdurrahman L., Behbehani G., Arececi R.J.
Dev. Dyn. 221:92-105(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: C57BL/6.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Embryo and Heart.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J and FVB/N.
Tissue: Egg and Mammary gland.
[5]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 31-38, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[6]"Characterization of gene expression, genomic structure, and chromosomal localization of Hells (Lsh)."
Geiman T.M., Durum S.K., Muegge K.
Genomics 54:477-483(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Lsh, an SNF2/helicase family member, is required for proliferation of mature T lymphocytes."
Geiman T.M., Muegge K.
Proc. Natl. Acad. Sci. U.S.A. 97:4772-4777(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
[8]"Lsh, a SNF2 family member, is required for normal murine development."
Geiman T.M., Tessarollo L., Anver M.R., Kopp J.B., Ward J.M., Muegge K.
Biochim. Biophys. Acta 1526:211-220(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[9]"Lsh, a member of the SNF2 family, is required for genome-wide methylation."
Dennis K., Fan T., Geiman T., Yan Q., Muegge K.
Genes Dev. 15:2940-2944(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Lsh, a modulator of CpG methylation, is crucial for normal histone methylation."
Yan Q., Huang J., Fan T., Zhu H., Muegge K.
EMBO J. 22:5154-5162(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]"Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin."
Yan Q., Cho E., Lockett S., Muegge K.
Mol. Cell. Biol. 23:8416-8428(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[12]"Growth retardation and premature aging phenotypes in mice with disruption of the SNF2-like gene, PASG."
Sun L.-Q., Lee D.W., Zhang Q., Xiao W., Raabe E.H., Meeker A., Miao D., Huso D.L., Arceci R.J.
Genes Dev. 18:1035-1046(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Lsh controls silencing of the imprinted Cdkn1c gene."
Fan T., Hagan J.P., Kozlov S.V., Stewart C.L., Muegge K.
Development 132:635-644(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25691 mRNA. Translation: AAB08015.2.
AF155210 mRNA. Translation: AAG43373.1.
AK013266 mRNA. Translation: BAB28757.2.
AK147126 mRNA. Translation: BAE27697.1.
BC020056 mRNA. Translation: AAH20056.1. Different initiation.
BC100394 mRNA. Translation: AAI00395.1.
PIRJC4666.
RefSeqNP_032260.2. NM_008234.3.
XP_006526762.1. XM_006526699.1.
UniGeneMm.392920.
Mm.486446.
Mm.57223.

3D structure databases

ProteinModelPortalQ60848.
SMRQ60848. Positions 175-789.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200271. 4 interactions.
DIPDIP-43735N.
IntActQ60848. 4 interactions.
MINTMINT-2521001.

PTM databases

PhosphoSiteQ60848.

Proteomic databases

PaxDbQ60848.
PRIDEQ60848.

Protocols and materials databases

DNASU15201.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025965; ENSMUSP00000025965; ENSMUSG00000025001. [Q60848-1]
GeneID15201.
KEGGmmu:15201.
UCSCuc008hjt.1. mouse. [Q60848-1]

Organism-specific databases

CTD3070.
MGIMGI:106209. Hells.

Phylogenomic databases

eggNOGCOG0553.
GeneTreeENSGT00740000115593.
HOGENOMHOG000172362.
HOVERGENHBG060049.
InParanoidQ60848.
OMAWDRESVD.
OrthoDBEOG7TXKG5.
PhylomeDBQ60848.
TreeFamTF329077.

Gene expression databases

ArrayExpressQ60848.
BgeeQ60848.
CleanExMM_HELLS.
GenevestigatorQ60848.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287739.
PROQ60848.
SOURCESearch...

Entry information

Entry nameHELLS_MOUSE
AccessionPrimary (citable) accession number: Q60848
Secondary accession number(s): Q497T3, Q8VDZ1, Q9CYV7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot