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Q60848

- HELLS_MOUSE

UniProt

Q60848 - HELLS_MOUSE

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Protein

Lymphocyte-specific helicase

Gene

Hells

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.9 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi231 – 2388ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. chromatin binding Source: MGI
  3. DNA binding Source: InterPro
  4. helicase activity Source: UniProtKB-KW

GO - Biological processi

  1. chromatin silencing Source: MGI
  2. DNA methylation Source: MGI
  3. lymphocyte differentiation Source: MGI
  4. lymphocyte proliferation Source: UniProtKB
  5. maintenance of DNA methylation Source: UniProtKB
  6. methylation-dependent chromatin silencing Source: UniProtKB
  7. mitotic nuclear division Source: UniProtKB-KW
  8. multicellular organismal development Source: UniProtKB
  9. negative regulation of apoptotic process Source: MGI
  10. negative regulation of intrinsic apoptotic signaling pathway Source: MGI
  11. pericentric heterochromatin assembly Source: UniProtKB
  12. transcription, DNA-templated Source: UniProtKB-KW
  13. urogenital system development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphocyte-specific helicase (EC:3.6.4.-)
Alternative name(s):
Proliferation-associated SNF2-like protein
Gene namesi
Name:HellsImported
Synonyms:Lsh1 Publication, PasgImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:106209. Hells.

Subcellular locationi

Nucleus 3 Publications
Note: Closely associated with pericentric heterochromatin.

GO - Cellular componenti

  1. chromosome, centromeric region Source: UniProtKB
  2. nucleus Source: MGI
  3. pericentric heterochromatin Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 821821Lymphocyte-specific helicasePRO_0000260052Add
BLAST

Proteomic databases

MaxQBiQ60848.
PaxDbiQ60848.
PRIDEiQ60848.

PTM databases

PhosphoSiteiQ60848.

Expressioni

Tissue specificityi

Highly expressed in thymus, bone marrow, and testis. Not detected in heart, brain, liver, kidney, skeletal muscle, spleen, peripheral blood lymphocytes, small intestine, colon, prostate or ovary.3 Publications

Developmental stagei

Differentially expressed during embryonic development, with highest expression in developing face, limbs, skeletal muscle, heart, and tail. Highly expressed in fetal thymocytes from day 14 to 16 of gestation, and expressed at much lower levels in adult thymus.3 Publications

Inductioni

By concanavalin or lipopolysaccharide in unactivated splenocytes.2 Publications

Gene expression databases

BgeeiQ60848.
CleanExiMM_HELLS.
ExpressionAtlasiQ60848. baseline and differential.
GenevestigatoriQ60848.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Dnmt3aO885084EBI-3043887,EBI-995154
Dnmt3bO885094EBI-3043887,EBI-7987547

Protein-protein interaction databases

BioGridi200271. 5 interactions.
DIPiDIP-43735N.
IntActiQ60848. 4 interactions.
MINTiMINT-2521001.

Structurei

3D structure databases

ProteinModelPortaliQ60848.
SMRiQ60848. Positions 175-739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini218 – 386169Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini586 – 736151Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili14 – 9683Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi337 – 3404DEAH boxSequence Analysis

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00740000115593.
HOGENOMiHOG000172362.
HOVERGENiHBG060049.
InParanoidiQ60848.
OMAiWDRESVD.
OrthoDBiEOG7TXKG5.
PhylomeDBiQ60848.
TreeFamiTF329077.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 12 Publications (identifier: Q60848-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEQTEPAVI TPAMLEEEEQ LEAAGLEKER KMLEEAQKSW DRESTEIRYR
60 70 80 90 100
RLQHLLEKSN IYSKFLLTKM EQQQLEEQKK KEKLEKKKRS LKLTEGKSLV
110 120 130 140 150
DGNGEKPVMK KKRGREDESY NISEVMSKEE ILSVAKKHKD NEDESSSTTS
160 170 180 190 200
LCVEDIQKNK DSNSMIKDRL SQTVRQNSKF FFDPVRKCNG QPVPFQQPKH
210 220 230 240 250
FTGGVMRWYQ VEGMEWLRML WENGINGILA DEMGLGKTVQ CIATIALMIQ
260 270 280 290 300
RGVPGPFLVC GPLSTLPNWM AEFKRFTPEI PTLLYHGTRE DRRKLVKNIH
310 320 330 340 350
KRQGTLQIHP VVVTSFEIAM RDQNALQHCY WKYLIVDEGH RIKNMKCRLI
360 370 380 390 400
RELKRFNADN KLLLTGTPLQ NNLSELWSLL NFLLPDVFDD LKSFESWFDI
410 420 430 440 450
TSLSETAEDI IAKEREQNVL HMLHQILTPF LLRRLKSDVA LEVPPKREVV
460 470 480 490 500
VYAPLCNKQE IFYTAIVNRT IANMFGSCEK ETVELSPTGR PKRRSRKSIN
510 520 530 540 550
YSELDQFPSE LEKLISQIQP EVNRERTVVE GNIPIESEVN LKLRNIMMLL
560 570 580 590 600
RKCCNHPYMI EYPIDPVTQE FKIDEELVTN SGKFLILDRM LPELKKRGHK
610 620 630 640 650
VLVFSQMTSM LDILMDYCHL RNFIFSRLDG SMSYSEREKN IYSFNTDPDV
660 670 680 690 700
FLFLVSTRAG GLGINLTAAD TVIIYDSDWN PQSDLQAQDR CHRIGQTKPV
710 720 730 740 750
VVYRLVTANT IDQKIVERAA AKRKLEKLII HKNHFKGGQS GLSQSKNFLD
760 770 780 790 800
AKELMELLKS RDYEREVKGS REKVISDEDL ELLLDRSDLI DQMKASRPIK
810 820
GKTGIFKILE NSEDSSAECL F
Length:821
Mass (Da):95,126
Last modified:March 1, 2001 - v2
Checksum:i8DEC2F2EEFBBD2F5
GO
Isoform 21 Publication (identifier: Q60848-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:808
Mass (Da):93,786
Checksum:iDBF5E847C1209568
GO

Sequence cautioni

The sequence AAH20056.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti497 – 4971K → R in AAI00395. (PubMed:15489334)Curated
Sequence conflicti620 – 6201L → P in AAI00395. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1313Missing in isoform 2. 1 PublicationVSP_052235Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25691 mRNA. Translation: AAB08015.2.
AF155210 mRNA. Translation: AAG43373.1.
AK013266 mRNA. Translation: BAB28757.2.
AK147126 mRNA. Translation: BAE27697.1.
BC020056 mRNA. Translation: AAH20056.1. Different initiation.
BC100394 mRNA. Translation: AAI00395.1.
CCDSiCCDS29789.1. [Q60848-1]
PIRiJC4666.
RefSeqiNP_032260.2. NM_008234.3. [Q60848-1]
XP_006526762.1. XM_006526699.1. [Q60848-1]
UniGeneiMm.392920.
Mm.486446.
Mm.57223.

Genome annotation databases

EnsembliENSMUST00000025965; ENSMUSP00000025965; ENSMUSG00000025001. [Q60848-1]
GeneIDi15201.
KEGGimmu:15201.
UCSCiuc008hjt.1. mouse. [Q60848-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25691 mRNA. Translation: AAB08015.2 .
AF155210 mRNA. Translation: AAG43373.1 .
AK013266 mRNA. Translation: BAB28757.2 .
AK147126 mRNA. Translation: BAE27697.1 .
BC020056 mRNA. Translation: AAH20056.1 . Different initiation.
BC100394 mRNA. Translation: AAI00395.1 .
CCDSi CCDS29789.1. [Q60848-1 ]
PIRi JC4666.
RefSeqi NP_032260.2. NM_008234.3. [Q60848-1 ]
XP_006526762.1. XM_006526699.1. [Q60848-1 ]
UniGenei Mm.392920.
Mm.486446.
Mm.57223.

3D structure databases

ProteinModelPortali Q60848.
SMRi Q60848. Positions 175-739.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200271. 5 interactions.
DIPi DIP-43735N.
IntActi Q60848. 4 interactions.
MINTi MINT-2521001.

PTM databases

PhosphoSitei Q60848.

Proteomic databases

MaxQBi Q60848.
PaxDbi Q60848.
PRIDEi Q60848.

Protocols and materials databases

DNASUi 15201.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025965 ; ENSMUSP00000025965 ; ENSMUSG00000025001 . [Q60848-1 ]
GeneIDi 15201.
KEGGi mmu:15201.
UCSCi uc008hjt.1. mouse. [Q60848-1 ]

Organism-specific databases

CTDi 3070.
MGIi MGI:106209. Hells.

Phylogenomic databases

eggNOGi COG0553.
GeneTreei ENSGT00740000115593.
HOGENOMi HOG000172362.
HOVERGENi HBG060049.
InParanoidi Q60848.
OMAi WDRESVD.
OrthoDBi EOG7TXKG5.
PhylomeDBi Q60848.
TreeFami TF329077.

Miscellaneous databases

NextBioi 287739.
PROi Q60848.
SOURCEi Search...

Gene expression databases

Bgeei Q60848.
CleanExi MM_HELLS.
ExpressionAtlasi Q60848. baseline and differential.
Genevestigatori Q60848.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view ]
Pfami PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE, INDUCTION.
    Strain: C57BL/6Imported.
    Tissue: Fetal thymusImported.
  2. "An SNF2 factor involved in mammalian development and cellular proliferation."
    Raabe E.H., Abdurrahman L., Behbehani G., Arececi R.J.
    Dev. Dyn. 221:92-105(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6Imported.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6JImported.
    Tissue: EmbryoImported and HeartImported.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6JImported and FVB/NImported.
    Tissue: EggImported and Mammary glandImported.
  5. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 31-38, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  6. "Characterization of gene expression, genomic structure, and chromosomal localization of Hells (Lsh)."
    Geiman T.M., Durum S.K., Muegge K.
    Genomics 54:477-483(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Lsh, an SNF2/helicase family member, is required for proliferation of mature T lymphocytes."
    Geiman T.M., Muegge K.
    Proc. Natl. Acad. Sci. U.S.A. 97:4772-4777(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
  8. "Lsh, a SNF2 family member, is required for normal murine development."
    Geiman T.M., Tessarollo L., Anver M.R., Kopp J.B., Ward J.M., Muegge K.
    Biochim. Biophys. Acta 1526:211-220(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  9. "Lsh, a member of the SNF2 family, is required for genome-wide methylation."
    Dennis K., Fan T., Geiman T., Yan Q., Muegge K.
    Genes Dev. 15:2940-2944(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Lsh, a modulator of CpG methylation, is crucial for normal histone methylation."
    Yan Q., Huang J., Fan T., Zhu H., Muegge K.
    EMBO J. 22:5154-5162(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. "Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin."
    Yan Q., Cho E., Lockett S., Muegge K.
    Mol. Cell. Biol. 23:8416-8428(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. "Growth retardation and premature aging phenotypes in mice with disruption of the SNF2-like gene, PASG."
    Sun L.-Q., Lee D.W., Zhang Q., Xiao W., Raabe E.H., Meeker A., Miao D., Huso D.L., Arceci R.J.
    Genes Dev. 18:1035-1046(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Lsh controls silencing of the imprinted Cdkn1c gene."
    Fan T., Hagan J.P., Kozlov S.V., Stewart C.L., Muegge K.
    Development 132:635-644(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiHELLS_MOUSE
AccessioniPrimary (citable) accession number: Q60848
Secondary accession number(s): Q497T3, Q8VDZ1, Q9CYV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3