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Protein

Krueppel-like factor 2

Gene

Klf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the CACCC box in the beta-globin gene promoter and activates transcription.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri271 – 29525C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri301 – 32525C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri331 – 35323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. sequence-specific DNA binding Source: Ensembl
  4. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cell morphogenesis Source: MGI
  2. cellular response to cycloheximide Source: Ensembl
  3. cellular response to hydrogen peroxide Source: Ensembl
  4. cellular response to interleukin-1 Source: Ensembl
  5. cellular response to laminar fluid shear stress Source: MGI
  6. cellular response to peptide Source: Ensembl
  7. cellular response to tumor necrosis factor Source: Ensembl
  8. erythrocyte homeostasis Source: MGI
  9. erythrocyte maturation Source: MGI
  10. in utero embryonic development Source: MGI
  11. multicellular organism growth Source: MGI
  12. negative regulation of interleukin-6 production Source: Ensembl
  13. positive regulation of protein metabolic process Source: MGI
  14. positive regulation of transcription, DNA-templated Source: MGI
  15. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  16. positive regulation of transcription from RNA polymerase II promoter in response to stress Source: MGI
  17. regulation of gene expression, epigenetic Source: MGI
  18. transcription, DNA-templated Source: UniProtKB-KW
  19. Type I pneumocyte differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 2
Alternative name(s):
Lung krueppel-like factor
Gene namesi
Name:Klf2
Synonyms:Lklf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1342772. Klf2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi121 – 1211K → R: Greatly impairs polyubiquitination. Abolishes polyubiquitination; when associated with R-146. 1 Publication
Mutagenesisi146 – 1461K → R: Abolishes polyubiquitination; when associated with R-121. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Krueppel-like factor 2PRO_0000047163Add
BLAST

Post-translational modificationi

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ60843.

PTM databases

PhosphoSiteiQ60843.

Expressioni

Tissue specificityi

Predominant expression in the lungs and spleen.

Gene expression databases

BgeeiQ60843.
CleanExiMM_KLF2.
GenevestigatoriQ60843.

Interactioni

Subunit structurei

Interacts with WWP1.By similarity

Protein-protein interaction databases

BioGridi200964. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ60843.
SMRiQ60843. Positions 269-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 267158Interaction with WWP1Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 7011Poly-ProAdd
BLAST
Compositional biasi165 – 1695Poly-Pro
Compositional biasi224 – 2307Poly-Ala

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri271 – 29525C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri301 – 32525C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri331 – 35323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000060173.
HOVERGENiHBG006220.
InParanoidiQ60843.
KOiK17845.
OMAiFYYPEPG.
OrthoDBiEOG747PJ4.
TreeFamiTF350556.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSEPILPS FATFASPCER GLQERWPRNE PEAGGTDEDL NNVLDFILSM
60 70 80 90 100
GLDGLGAENP PEPPPQPPPP AFYYPEPGAP PPYSIPAASL GTELLRPDLD
110 120 130 140 150
PPQGPALHGR FLLAPPGRLV KAEPPEVDGG GYGCAPGLAH GPRGLKLEGA
160 170 180 190 200
PGATGACMRG PAGRPPPPPD TPPLSPDGPL RIPASGPRNP FPPPFGPGPS
210 220 230 240 250
FGGPGPALHY GPPAPGAFGL FEDAAAAAAA LGLAPPATRG LLTPPSSPLE
260 270 280 290 300
LLEAKPKRGR RSWPRKRAAT HTCSYTNCGK TYTKSSHLKA HLRTHTGEKP
310 320 330 340 350
YHCNWEGCGW KFARSDELTR HYRKHTGHRP FQCHLCDRAF SRSDHLALHM

KRHM
Length:354
Mass (Da):37,657
Last modified:July 27, 2011 - v2
Checksum:i31A99C00DFC5B810
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881A → D in AAA86728 (PubMed:7565748).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25096 mRNA. Translation: AAA86728.1.
BC138737 mRNA. Translation: AAI38738.1.
BC138738 mRNA. Translation: AAI38739.1.
CCDSiCCDS22412.1.
RefSeqiNP_032478.2. NM_008452.2.
UniGeneiMm.26938.

Genome annotation databases

EnsembliENSMUST00000067912; ENSMUSP00000064823; ENSMUSG00000055148.
GeneIDi16598.
KEGGimmu:16598.
UCSCiuc009mfq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25096 mRNA. Translation: AAA86728.1.
BC138737 mRNA. Translation: AAI38738.1.
BC138738 mRNA. Translation: AAI38739.1.
CCDSiCCDS22412.1.
RefSeqiNP_032478.2. NM_008452.2.
UniGeneiMm.26938.

3D structure databases

ProteinModelPortaliQ60843.
SMRiQ60843. Positions 269-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200964. 2 interactions.

PTM databases

PhosphoSiteiQ60843.

Proteomic databases

PRIDEiQ60843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067912; ENSMUSP00000064823; ENSMUSG00000055148.
GeneIDi16598.
KEGGimmu:16598.
UCSCiuc009mfq.2. mouse.

Organism-specific databases

CTDi10365.
MGIiMGI:1342772. Klf2.

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000060173.
HOVERGENiHBG006220.
InParanoidiQ60843.
KOiK17845.
OMAiFYYPEPG.
OrthoDBiEOG747PJ4.
TreeFamiTF350556.

Miscellaneous databases

NextBioi290169.
PROiQ60843.
SOURCEiSearch...

Gene expression databases

BgeeiQ60843.
CleanExiMM_KLF2.
GenevestigatoriQ60843.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a gene encoding a functional zinc finger protein homologous to erythroid Kruppel-like factor: identification of a new multigene family."
    Anderson K.P., Kern C.B., Crable S.C., Lingrel J.B.
    Mol. Cell. Biol. 15:5957-5965(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Lung Krueppel-like factor contains an autoinhibitory domain that regulates its transcriptional activation by binding WWP1, an E3 ubiquitin ligase."
    Conkright M.D., Wani M.A., Lingrel J.B.
    J. Biol. Chem. 276:29299-29306(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WWP1.
  4. "WWP1-dependent ubiquitination and degradation of the lung Kruppel-like factor, KLF2."
    Zhang X., Srinivasan S.V., Lingrel J.B.
    Biochem. Biophys. Res. Commun. 316:139-148(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WWP1, MUTAGENESIS OF LYS-121 AND LYS-146, UBIQUITINATION.

Entry informationi

Entry nameiKLF2_MOUSE
AccessioniPrimary (citable) accession number: Q60843
Secondary accession number(s): B2RS60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.