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Q60843

- KLF2_MOUSE

UniProt

Q60843 - KLF2_MOUSE

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Protein
Krueppel-like factor 2
Gene
Klf2, Lklf
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to the CACCC box in the beta-globin gene promoter and activates transcription.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri271 – 29525C2H2-type 1
Add
BLAST
Zinc fingeri301 – 32525C2H2-type 2
Add
BLAST
Zinc fingeri331 – 35323C2H2-type 3
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: UniProtKB
  4. sequence-specific DNA binding Source: Ensembl
  5. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. Type I pneumocyte differentiation Source: MGI
  2. cell morphogenesis Source: MGI
  3. cellular response to cycloheximide Source: Ensembl
  4. cellular response to hydrogen peroxide Source: Ensembl
  5. cellular response to interleukin-1 Source: Ensembl
  6. cellular response to laminar fluid shear stress Source: Ensembl
  7. cellular response to peptide Source: Ensembl
  8. cellular response to tumor necrosis factor Source: Ensembl
  9. erythrocyte homeostasis Source: MGI
  10. erythrocyte maturation Source: MGI
  11. in utero embryonic development Source: MGI
  12. multicellular organism growth Source: MGI
  13. negative regulation of interleukin-6 production Source: Ensembl
  14. positive regulation of protein metabolic process Source: Ensembl
  15. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  16. positive regulation of transcription from RNA polymerase II promoter in response to stress Source: Ensembl
  17. positive regulation of transcription, DNA-templated Source: MGI
  18. regulation of gene expression, epigenetic Source: MGI
  19. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 2
Alternative name(s):
Lung krueppel-like factor
Gene namesi
Name:Klf2
Synonyms:Lklf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1342772. Klf2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi121 – 1211K → R: Greatly impairs polyubiquitination. Abolishes polyubiquitination; when associated with R-146. 1 Publication
Mutagenesisi146 – 1461K → R: Abolishes polyubiquitination; when associated with R-121. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Krueppel-like factor 2
PRO_0000047163Add
BLAST

Post-translational modificationi

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ60843.

PTM databases

PhosphoSiteiQ60843.

Expressioni

Tissue specificityi

Predominant expression in the lungs and spleen.

Gene expression databases

BgeeiQ60843.
CleanExiMM_KLF2.
GenevestigatoriQ60843.

Interactioni

Subunit structurei

Interacts with WWP1 By similarity.2 Publications

Protein-protein interaction databases

BioGridi200964. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ60843.
SMRiQ60843. Positions 269-353.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 267158Interaction with WWP1
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 7011Poly-Pro
Add
BLAST
Compositional biasi165 – 1695Poly-Pro
Compositional biasi224 – 2307Poly-Ala

Sequence similaritiesi

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00750000117252.
HOGENOMiHOG000060173.
HOVERGENiHBG006220.
InParanoidiB2RS60.
KOiK17845.
OMAiFYYPEPG.
OrthoDBiEOG747PJ4.
TreeFamiTF350556.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60843-1 [UniParc]FASTAAdd to Basket

« Hide

MALSEPILPS FATFASPCER GLQERWPRNE PEAGGTDEDL NNVLDFILSM    50
GLDGLGAENP PEPPPQPPPP AFYYPEPGAP PPYSIPAASL GTELLRPDLD 100
PPQGPALHGR FLLAPPGRLV KAEPPEVDGG GYGCAPGLAH GPRGLKLEGA 150
PGATGACMRG PAGRPPPPPD TPPLSPDGPL RIPASGPRNP FPPPFGPGPS 200
FGGPGPALHY GPPAPGAFGL FEDAAAAAAA LGLAPPATRG LLTPPSSPLE 250
LLEAKPKRGR RSWPRKRAAT HTCSYTNCGK TYTKSSHLKA HLRTHTGEKP 300
YHCNWEGCGW KFARSDELTR HYRKHTGHRP FQCHLCDRAF SRSDHLALHM 350
KRHM 354
Length:354
Mass (Da):37,657
Last modified:July 27, 2011 - v2
Checksum:i31A99C00DFC5B810
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881A → D in AAA86728. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25096 mRNA. Translation: AAA86728.1.
BC138737 mRNA. Translation: AAI38738.1.
BC138738 mRNA. Translation: AAI38739.1.
CCDSiCCDS22412.1.
RefSeqiNP_032478.2. NM_008452.2.
UniGeneiMm.26938.

Genome annotation databases

EnsembliENSMUST00000067912; ENSMUSP00000064823; ENSMUSG00000055148.
GeneIDi16598.
KEGGimmu:16598.
UCSCiuc009mfq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25096 mRNA. Translation: AAA86728.1 .
BC138737 mRNA. Translation: AAI38738.1 .
BC138738 mRNA. Translation: AAI38739.1 .
CCDSi CCDS22412.1.
RefSeqi NP_032478.2. NM_008452.2.
UniGenei Mm.26938.

3D structure databases

ProteinModelPortali Q60843.
SMRi Q60843. Positions 269-353.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200964. 1 interaction.

PTM databases

PhosphoSitei Q60843.

Proteomic databases

PRIDEi Q60843.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000067912 ; ENSMUSP00000064823 ; ENSMUSG00000055148 .
GeneIDi 16598.
KEGGi mmu:16598.
UCSCi uc009mfq.2. mouse.

Organism-specific databases

CTDi 10365.
MGIi MGI:1342772. Klf2.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00750000117252.
HOGENOMi HOG000060173.
HOVERGENi HBG006220.
InParanoidi B2RS60.
KOi K17845.
OMAi FYYPEPG.
OrthoDBi EOG747PJ4.
TreeFami TF350556.

Miscellaneous databases

NextBioi 290169.
PROi Q60843.
SOURCEi Search...

Gene expression databases

Bgeei Q60843.
CleanExi MM_KLF2.
Genevestigatori Q60843.

Family and domain databases

Gene3Di 3.30.160.60. 3 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 3 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a gene encoding a functional zinc finger protein homologous to erythroid Kruppel-like factor: identification of a new multigene family."
    Anderson K.P., Kern C.B., Crable S.C., Lingrel J.B.
    Mol. Cell. Biol. 15:5957-5965(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Lung Krueppel-like factor contains an autoinhibitory domain that regulates its transcriptional activation by binding WWP1, an E3 ubiquitin ligase."
    Conkright M.D., Wani M.A., Lingrel J.B.
    J. Biol. Chem. 276:29299-29306(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WWP1.
  4. "WWP1-dependent ubiquitination and degradation of the lung Kruppel-like factor, KLF2."
    Zhang X., Srinivasan S.V., Lingrel J.B.
    Biochem. Biophys. Res. Commun. 316:139-148(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WWP1, MUTAGENESIS OF LYS-121 AND LYS-146, UBIQUITINATION.

Entry informationi

Entry nameiKLF2_MOUSE
AccessioniPrimary (citable) accession number: Q60843
Secondary accession number(s): B2RS60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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