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Q60843 (KLF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Krueppel-like factor 2
Alternative name(s):
Lung krueppel-like factor
Gene names
Name:Klf2
Synonyms:Lklf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to the CACCC box in the beta-globin gene promoter and activates transcription.

Subunit structure

Interacts with WWP1 By similarity. Ref.3 Ref.4

Subcellular location

Nucleus By similarity.

Tissue specificity

Predominant expression in the lungs and spleen.

Post-translational modification

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. Ref.4

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processType I pneumocyte differentiation

Inferred from mutant phenotype PubMed 22001906. Source: MGI

cell morphogenesis

Inferred from mutant phenotype PubMed 15947087. Source: MGI

cellular response to cycloheximide

Inferred from electronic annotation. Source: Ensembl

cellular response to hydrogen peroxide

Inferred from electronic annotation. Source: Ensembl

cellular response to interleukin-1

Inferred from electronic annotation. Source: Ensembl

cellular response to laminar fluid shear stress

Inferred from electronic annotation. Source: Ensembl

cellular response to peptide

Inferred from electronic annotation. Source: Ensembl

cellular response to tumor necrosis factor

Inferred from electronic annotation. Source: Ensembl

erythrocyte homeostasis

Inferred from mutant phenotype PubMed 15947087. Source: MGI

erythrocyte maturation

Inferred from mutant phenotype PubMed 15947087. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 15947087PubMed 9859212. Source: MGI

multicellular organism growth

Inferred from mutant phenotype PubMed 15947087. Source: MGI

negative regulation of interleukin-6 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein metabolic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 22001906. Source: MGI

positive regulation of transcription from RNA polymerase II promoter in response to stress

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.1. Source: MGI

regulation of gene expression, epigenetic

Inferred from genetic interaction PubMed 22482507. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 22001906. Source: MGI

   Molecular_functionDNA binding

Inferred from sequence or structural similarity PubMed 15820306. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction Ref.3. Source: UniProtKB

sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding transcription factor activity

Inferred from sequence alignment Ref.1. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Krueppel-like factor 2
PRO_0000047163

Regions

Zinc finger271 – 29525C2H2-type 1
Zinc finger301 – 32525C2H2-type 2
Zinc finger331 – 35323C2H2-type 3
Region110 – 267158Interaction with WWP1
Compositional bias60 – 7011Poly-Pro
Compositional bias165 – 1695Poly-Pro
Compositional bias224 – 2307Poly-Ala

Experimental info

Mutagenesis1211K → R: Greatly impairs polyubiquitination. Abolishes polyubiquitination; when associated with R-146. Ref.4
Mutagenesis1461K → R: Abolishes polyubiquitination; when associated with R-121. Ref.4
Sequence conflict881A → D in AAA86728. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q60843 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 31A99C00DFC5B810

FASTA35437,657
        10         20         30         40         50         60 
MALSEPILPS FATFASPCER GLQERWPRNE PEAGGTDEDL NNVLDFILSM GLDGLGAENP 

        70         80         90        100        110        120 
PEPPPQPPPP AFYYPEPGAP PPYSIPAASL GTELLRPDLD PPQGPALHGR FLLAPPGRLV 

       130        140        150        160        170        180 
KAEPPEVDGG GYGCAPGLAH GPRGLKLEGA PGATGACMRG PAGRPPPPPD TPPLSPDGPL 

       190        200        210        220        230        240 
RIPASGPRNP FPPPFGPGPS FGGPGPALHY GPPAPGAFGL FEDAAAAAAA LGLAPPATRG 

       250        260        270        280        290        300 
LLTPPSSPLE LLEAKPKRGR RSWPRKRAAT HTCSYTNCGK TYTKSSHLKA HLRTHTGEKP 

       310        320        330        340        350 
YHCNWEGCGW KFARSDELTR HYRKHTGHRP FQCHLCDRAF SRSDHLALHM KRHM 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of a gene encoding a functional zinc finger protein homologous to erythroid Kruppel-like factor: identification of a new multigene family."
Anderson K.P., Kern C.B., Crable S.C., Lingrel J.B.
Mol. Cell. Biol. 15:5957-5965(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X CBA.
Tissue: Lung.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Lung Krueppel-like factor contains an autoinhibitory domain that regulates its transcriptional activation by binding WWP1, an E3 ubiquitin ligase."
Conkright M.D., Wani M.A., Lingrel J.B.
J. Biol. Chem. 276:29299-29306(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH WWP1.
[4]"WWP1-dependent ubiquitination and degradation of the lung Kruppel-like factor, KLF2."
Zhang X., Srinivasan S.V., Lingrel J.B.
Biochem. Biophys. Res. Commun. 316:139-148(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH WWP1, MUTAGENESIS OF LYS-121 AND LYS-146, UBIQUITINATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25096 mRNA. Translation: AAA86728.1.
BC138737 mRNA. Translation: AAI38738.1.
BC138738 mRNA. Translation: AAI38739.1.
CCDSCCDS22412.1.
RefSeqNP_032478.2. NM_008452.2.
UniGeneMm.26938.

3D structure databases

ProteinModelPortalQ60843.
SMRQ60843. Positions 269-353.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200964. 1 interaction.

PTM databases

PhosphoSiteQ60843.

Proteomic databases

PRIDEQ60843.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000067912; ENSMUSP00000064823; ENSMUSG00000055148.
GeneID16598.
KEGGmmu:16598.
UCSCuc009mfq.2. mouse.

Organism-specific databases

CTD10365.
MGIMGI:1342772. Klf2.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00750000117252.
HOGENOMHOG000060173.
HOVERGENHBG006220.
InParanoidB2RS60.
KOK17845.
OMAFYYPEPG.
OrthoDBEOG747PJ4.
TreeFamTF350556.

Gene expression databases

BgeeQ60843.
CleanExMM_KLF2.
GenevestigatorQ60843.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio290169.
PROQ60843.
SOURCESearch...

Entry information

Entry nameKLF2_MOUSE
AccessionPrimary (citable) accession number: Q60843
Secondary accession number(s): B2RS60
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot