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Protein

Krueppel-like factor 2

Gene

Klf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri271 – 295C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri301 – 325C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri331 – 353C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

  • cell morphogenesis Source: MGI
  • cellular response to cycloheximide Source: Ensembl
  • cellular response to hydrogen peroxide Source: Ensembl
  • cellular response to interleukin-1 Source: Ensembl
  • cellular response to laminar fluid shear stress Source: MGI
  • cellular response to peptide Source: Ensembl
  • cellular response to tumor necrosis factor Source: Ensembl
  • erythrocyte homeostasis Source: MGI
  • erythrocyte maturation Source: MGI
  • in utero embryonic development Source: MGI
  • multicellular organism growth Source: MGI
  • negative regulation of interleukin-6 production Source: Ensembl
  • positive regulation of nitric oxide biosynthetic process Source: MGI
  • positive regulation of protein metabolic process Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter in response to stress Source: MGI
  • regulation of gene expression, epigenetic Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • Type I pneumocyte differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 2
Alternative name(s):
Lung krueppel-like factor
Gene namesi
Name:Klf2
Synonyms:Lklf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1342772. Klf2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi121K → R: Greatly impairs polyubiquitination. Abolishes polyubiquitination; when associated with R-146. 1 Publication1
Mutagenesisi146K → R: Abolishes polyubiquitination; when associated with R-121. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471631 – 354Krueppel-like factor 2Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171PhosphothreonineCombined sources1
Modified residuei243PhosphothreonineCombined sources1
Modified residuei246PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ60843.
PaxDbiQ60843.
PRIDEiQ60843.

PTM databases

iPTMnetiQ60843.
PhosphoSitePlusiQ60843.

Expressioni

Tissue specificityi

Predominant expression in the lungs and spleen.

Gene expression databases

BgeeiENSMUSG00000055148.
CleanExiMM_KLF2.
GenevisibleiQ60843. MM.

Interactioni

Subunit structurei

Interacts with WWP1.2 Publications

Protein-protein interaction databases

BioGridi200964. 2 interactors.
STRINGi10090.ENSMUSP00000064823.

Structurei

3D structure databases

ProteinModelPortaliQ60843.
SMRiQ60843.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni110 – 267Interaction with WWP1Add BLAST158

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi60 – 70Poly-ProAdd BLAST11
Compositional biasi165 – 169Poly-Pro5
Compositional biasi224 – 230Poly-Ala7

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri271 – 295C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri301 – 325C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri331 – 353C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000060173.
HOVERGENiHBG006220.
InParanoidiQ60843.
KOiK17845.
OMAiFYYPEPG.
OrthoDBiEOG091G1BN0.
TreeFamiTF350556.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSEPILPS FATFASPCER GLQERWPRNE PEAGGTDEDL NNVLDFILSM
60 70 80 90 100
GLDGLGAENP PEPPPQPPPP AFYYPEPGAP PPYSIPAASL GTELLRPDLD
110 120 130 140 150
PPQGPALHGR FLLAPPGRLV KAEPPEVDGG GYGCAPGLAH GPRGLKLEGA
160 170 180 190 200
PGATGACMRG PAGRPPPPPD TPPLSPDGPL RIPASGPRNP FPPPFGPGPS
210 220 230 240 250
FGGPGPALHY GPPAPGAFGL FEDAAAAAAA LGLAPPATRG LLTPPSSPLE
260 270 280 290 300
LLEAKPKRGR RSWPRKRAAT HTCSYTNCGK TYTKSSHLKA HLRTHTGEKP
310 320 330 340 350
YHCNWEGCGW KFARSDELTR HYRKHTGHRP FQCHLCDRAF SRSDHLALHM

KRHM
Length:354
Mass (Da):37,657
Last modified:July 27, 2011 - v2
Checksum:i31A99C00DFC5B810
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88A → D in AAA86728 (PubMed:7565748).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25096 mRNA. Translation: AAA86728.1.
BC138737 mRNA. Translation: AAI38738.1.
BC138738 mRNA. Translation: AAI38739.1.
CCDSiCCDS22412.1.
RefSeqiNP_032478.2. NM_008452.2.
UniGeneiMm.26938.

Genome annotation databases

EnsembliENSMUST00000067912; ENSMUSP00000064823; ENSMUSG00000055148.
GeneIDi16598.
KEGGimmu:16598.
UCSCiuc009mfq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25096 mRNA. Translation: AAA86728.1.
BC138737 mRNA. Translation: AAI38738.1.
BC138738 mRNA. Translation: AAI38739.1.
CCDSiCCDS22412.1.
RefSeqiNP_032478.2. NM_008452.2.
UniGeneiMm.26938.

3D structure databases

ProteinModelPortaliQ60843.
SMRiQ60843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200964. 2 interactors.
STRINGi10090.ENSMUSP00000064823.

PTM databases

iPTMnetiQ60843.
PhosphoSitePlusiQ60843.

Proteomic databases

MaxQBiQ60843.
PaxDbiQ60843.
PRIDEiQ60843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067912; ENSMUSP00000064823; ENSMUSG00000055148.
GeneIDi16598.
KEGGimmu:16598.
UCSCiuc009mfq.2. mouse.

Organism-specific databases

CTDi10365.
MGIiMGI:1342772. Klf2.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000060173.
HOVERGENiHBG006220.
InParanoidiQ60843.
KOiK17845.
OMAiFYYPEPG.
OrthoDBiEOG091G1BN0.
TreeFamiTF350556.

Miscellaneous databases

PROiQ60843.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000055148.
CleanExiMM_KLF2.
GenevisibleiQ60843. MM.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKLF2_MOUSE
AccessioniPrimary (citable) accession number: Q60843
Secondary accession number(s): B2RS60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.