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Q60843

- KLF2_MOUSE

UniProt

Q60843 - KLF2_MOUSE

Protein

Krueppel-like factor 2

Gene

Klf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Binds to the CACCC box in the beta-globin gene promoter and activates transcription.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri271 – 29525C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri301 – 32525C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri331 – 35323C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding Source: Ensembl
    5. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. cell morphogenesis Source: MGI
    2. cellular response to cycloheximide Source: Ensembl
    3. cellular response to hydrogen peroxide Source: Ensembl
    4. cellular response to interleukin-1 Source: Ensembl
    5. cellular response to laminar fluid shear stress Source: Ensembl
    6. cellular response to peptide Source: Ensembl
    7. cellular response to tumor necrosis factor Source: Ensembl
    8. erythrocyte homeostasis Source: MGI
    9. erythrocyte maturation Source: MGI
    10. in utero embryonic development Source: MGI
    11. multicellular organism growth Source: MGI
    12. negative regulation of interleukin-6 production Source: Ensembl
    13. positive regulation of protein metabolic process Source: Ensembl
    14. positive regulation of transcription, DNA-templated Source: MGI
    15. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    16. positive regulation of transcription from RNA polymerase II promoter in response to stress Source: Ensembl
    17. regulation of gene expression, epigenetic Source: MGI
    18. transcription, DNA-templated Source: UniProtKB-KW
    19. Type I pneumocyte differentiation Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Krueppel-like factor 2
    Alternative name(s):
    Lung krueppel-like factor
    Gene namesi
    Name:Klf2
    Synonyms:Lklf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1342772. Klf2.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi121 – 1211K → R: Greatly impairs polyubiquitination. Abolishes polyubiquitination; when associated with R-146. 1 Publication
    Mutagenesisi146 – 1461K → R: Abolishes polyubiquitination; when associated with R-121. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Krueppel-like factor 2PRO_0000047163Add
    BLAST

    Post-translational modificationi

    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiQ60843.

    PTM databases

    PhosphoSiteiQ60843.

    Expressioni

    Tissue specificityi

    Predominant expression in the lungs and spleen.

    Gene expression databases

    BgeeiQ60843.
    CleanExiMM_KLF2.
    GenevestigatoriQ60843.

    Interactioni

    Subunit structurei

    Interacts with WWP1.By similarity

    Protein-protein interaction databases

    BioGridi200964. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60843.
    SMRiQ60843. Positions 269-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni110 – 267158Interaction with WWP1Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi60 – 7011Poly-ProAdd
    BLAST
    Compositional biasi165 – 1695Poly-Pro
    Compositional biasi224 – 2307Poly-Ala

    Sequence similaritiesi

    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri271 – 29525C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri301 – 32525C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri331 – 35323C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00750000117252.
    HOGENOMiHOG000060173.
    HOVERGENiHBG006220.
    InParanoidiB2RS60.
    KOiK17845.
    OMAiFYYPEPG.
    OrthoDBiEOG747PJ4.
    TreeFamiTF350556.

    Family and domain databases

    Gene3Di3.30.160.60. 3 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60843-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALSEPILPS FATFASPCER GLQERWPRNE PEAGGTDEDL NNVLDFILSM    50
    GLDGLGAENP PEPPPQPPPP AFYYPEPGAP PPYSIPAASL GTELLRPDLD 100
    PPQGPALHGR FLLAPPGRLV KAEPPEVDGG GYGCAPGLAH GPRGLKLEGA 150
    PGATGACMRG PAGRPPPPPD TPPLSPDGPL RIPASGPRNP FPPPFGPGPS 200
    FGGPGPALHY GPPAPGAFGL FEDAAAAAAA LGLAPPATRG LLTPPSSPLE 250
    LLEAKPKRGR RSWPRKRAAT HTCSYTNCGK TYTKSSHLKA HLRTHTGEKP 300
    YHCNWEGCGW KFARSDELTR HYRKHTGHRP FQCHLCDRAF SRSDHLALHM 350
    KRHM 354
    Length:354
    Mass (Da):37,657
    Last modified:July 27, 2011 - v2
    Checksum:i31A99C00DFC5B810
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti88 – 881A → D in AAA86728. (PubMed:7565748)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25096 mRNA. Translation: AAA86728.1.
    BC138737 mRNA. Translation: AAI38738.1.
    BC138738 mRNA. Translation: AAI38739.1.
    CCDSiCCDS22412.1.
    RefSeqiNP_032478.2. NM_008452.2.
    UniGeneiMm.26938.

    Genome annotation databases

    EnsembliENSMUST00000067912; ENSMUSP00000064823; ENSMUSG00000055148.
    GeneIDi16598.
    KEGGimmu:16598.
    UCSCiuc009mfq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25096 mRNA. Translation: AAA86728.1 .
    BC138737 mRNA. Translation: AAI38738.1 .
    BC138738 mRNA. Translation: AAI38739.1 .
    CCDSi CCDS22412.1.
    RefSeqi NP_032478.2. NM_008452.2.
    UniGenei Mm.26938.

    3D structure databases

    ProteinModelPortali Q60843.
    SMRi Q60843. Positions 269-353.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200964. 1 interaction.

    PTM databases

    PhosphoSitei Q60843.

    Proteomic databases

    PRIDEi Q60843.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067912 ; ENSMUSP00000064823 ; ENSMUSG00000055148 .
    GeneIDi 16598.
    KEGGi mmu:16598.
    UCSCi uc009mfq.2. mouse.

    Organism-specific databases

    CTDi 10365.
    MGIi MGI:1342772. Klf2.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00750000117252.
    HOGENOMi HOG000060173.
    HOVERGENi HBG006220.
    InParanoidi B2RS60.
    KOi K17845.
    OMAi FYYPEPG.
    OrthoDBi EOG747PJ4.
    TreeFami TF350556.

    Miscellaneous databases

    NextBioi 290169.
    PROi Q60843.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q60843.
    CleanExi MM_KLF2.
    Genevestigatori Q60843.

    Family and domain databases

    Gene3Di 3.30.160.60. 3 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a gene encoding a functional zinc finger protein homologous to erythroid Kruppel-like factor: identification of a new multigene family."
      Anderson K.P., Kern C.B., Crable S.C., Lingrel J.B.
      Mol. Cell. Biol. 15:5957-5965(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Lung.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Lung Krueppel-like factor contains an autoinhibitory domain that regulates its transcriptional activation by binding WWP1, an E3 ubiquitin ligase."
      Conkright M.D., Wani M.A., Lingrel J.B.
      J. Biol. Chem. 276:29299-29306(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH WWP1.
    4. "WWP1-dependent ubiquitination and degradation of the lung Kruppel-like factor, KLF2."
      Zhang X., Srinivasan S.V., Lingrel J.B.
      Biochem. Biophys. Res. Commun. 316:139-148(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH WWP1, MUTAGENESIS OF LYS-121 AND LYS-146, UBIQUITINATION.

    Entry informationi

    Entry nameiKLF2_MOUSE
    AccessioniPrimary (citable) accession number: Q60843
    Secondary accession number(s): B2RS60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3