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Protein

Protein phosphatase 1 regulatory subunit 1B

Gene

Ppp1r1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of protein-phosphatase 1.

GO - Molecular functioni

  • protein phosphatase inhibitor activity Source: MGI
  • protein serine/threonine phosphatase inhibitor activity Source: MGI

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • negative regulation of female receptivity Source: MGI
  • negative regulation of protein kinase activity Source: GO_Central
  • response to amphetamine Source: MGI
  • transcription, DNA-templated Source: MGI
  • visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Enzyme and pathway databases

ReactomeiR-MMU-180024. DARPP-32 events.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 1B
Alternative name(s):
DARPP-32
Dopamine- and cAMP-regulated neuronal phosphoprotein
Gene namesi
Name:Ppp1r1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:94860. Ppp1r1b.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • neuronal cell body Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194Protein phosphatase 1 regulatory subunit 1BPRO_0000071474Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei34 – 341Phosphothreonine; by PKABy similarity
Modified residuei45 – 451PhosphoserineCombined sources
Modified residuei46 – 461PhosphoserineCombined sources
Modified residuei75 – 751PhosphothreonineCombined sources
Modified residuei97 – 971PhosphoserineCombined sources
Modified residuei130 – 1301PhosphoserineBy similarity
Modified residuei192 – 1921PhosphoserineCombined sources

Post-translational modificationi

Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.By similarity
Phosphorylation of Thr-34 is required for activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ60829.
PaxDbiQ60829.
PeptideAtlasiQ60829.
PRIDEiQ60829.

PTM databases

iPTMnetiQ60829.
PhosphoSiteiQ60829.

Expressioni

Gene expression databases

BgeeiQ60829.
GenevisibleiQ60829. MM.

Interactioni

Protein-protein interaction databases

IntActiQ60829. 1 interaction.
MINTiMINT-4098740.
STRINGi10090.ENSMUSP00000077760.

Structurei

3D structure databases

ProteinModelPortaliQ60829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410J1BH. Eukaryota.
ENOG4111TN1. LUCA.
GeneTreeiENSGT00730000111283.
HOGENOMiHOG000290193.
HOVERGENiHBG099182.
InParanoidiQ60829.
KOiK15494.
OMAiPAMLFRV.
OrthoDBiEOG7PCJJG.
PhylomeDBiQ60829.
TreeFamiTF332576.

Family and domain databases

InterProiIPR015670. DARPP-32.
IPR008466. PPP1R1A/B/C.
[Graphical view]
PANTHERiPTHR15417. PTHR15417. 1 hit.
PTHR15417:SF2. PTHR15417:SF2. 1 hit.
PfamiPF05395. DARPP-32. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60829-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPAMLFR VSEHSSPEEE
60 70 80 90 100
ASPHQRTSGE GHHPKSKRPN PCAYTPPSLK AVQHLQTISN LSENQASEEE
110 120 130 140 150
DELGELRELG YPQEDDEEDE DEEEDEEEDS QAEVLKGSRG TVGQKPTCGR
160 170 180 190
GLEGPWERPP PLDEPQRDGN SEDQVEGRAT LSEPGEEPQH PSPP
Length:194
Mass (Da):21,781
Last modified:October 10, 2002 - v2
Checksum:iE0509291E6615EA3
GO
Isoform 2 (identifier: Q60829-2) [UniParc]FASTAAdd to basket

Also known as: DARPP-30

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Show »
Length:158
Mass (Da):17,556
Checksum:i33141573EA030243
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3636Missing in isoform 2. 1 PublicationVSP_025058Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY640620 mRNA. Translation: AAT66920.1.
AY640621 mRNA. Translation: AAT66921.1.
AL591390 Genomic DNA. Translation: CAM22017.1.
BC011122 mRNA. Translation: AAH11122.1.
BC026568 mRNA. Translation: AAH26568.1.
BC031129 mRNA. Translation: AAH31129.1.
U23160 Genomic DNA. Translation: AAA93223.1.
AK141563 mRNA. Translation: BAE24736.1.
CCDSiCCDS25344.1. [Q60829-1]
RefSeqiNP_001300899.1. NM_001313970.1. [Q60829-2]
NP_659077.1. NM_144828.2. [Q60829-1]
UniGeneiMm.45372.

Genome annotation databases

EnsembliENSMUST00000078694; ENSMUSP00000077760; ENSMUSG00000061718. [Q60829-1]
ENSMUST00000137634; ENSMUSP00000123528; ENSMUSG00000061718. [Q60829-2]
ENSMUST00000150762; ENSMUSP00000121147; ENSMUSG00000061718. [Q60829-2]
GeneIDi19049.
KEGGimmu:19049.
UCSCiuc007lfz.1. mouse. [Q60829-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY640620 mRNA. Translation: AAT66920.1.
AY640621 mRNA. Translation: AAT66921.1.
AL591390 Genomic DNA. Translation: CAM22017.1.
BC011122 mRNA. Translation: AAH11122.1.
BC026568 mRNA. Translation: AAH26568.1.
BC031129 mRNA. Translation: AAH31129.1.
U23160 Genomic DNA. Translation: AAA93223.1.
AK141563 mRNA. Translation: BAE24736.1.
CCDSiCCDS25344.1. [Q60829-1]
RefSeqiNP_001300899.1. NM_001313970.1. [Q60829-2]
NP_659077.1. NM_144828.2. [Q60829-1]
UniGeneiMm.45372.

3D structure databases

ProteinModelPortaliQ60829.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ60829. 1 interaction.
MINTiMINT-4098740.
STRINGi10090.ENSMUSP00000077760.

PTM databases

iPTMnetiQ60829.
PhosphoSiteiQ60829.

Proteomic databases

MaxQBiQ60829.
PaxDbiQ60829.
PeptideAtlasiQ60829.
PRIDEiQ60829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078694; ENSMUSP00000077760; ENSMUSG00000061718. [Q60829-1]
ENSMUST00000137634; ENSMUSP00000123528; ENSMUSG00000061718. [Q60829-2]
ENSMUST00000150762; ENSMUSP00000121147; ENSMUSG00000061718. [Q60829-2]
GeneIDi19049.
KEGGimmu:19049.
UCSCiuc007lfz.1. mouse. [Q60829-1]

Organism-specific databases

CTDi84152.
MGIiMGI:94860. Ppp1r1b.

Phylogenomic databases

eggNOGiENOG410J1BH. Eukaryota.
ENOG4111TN1. LUCA.
GeneTreeiENSGT00730000111283.
HOGENOMiHOG000290193.
HOVERGENiHBG099182.
InParanoidiQ60829.
KOiK15494.
OMAiPAMLFRV.
OrthoDBiEOG7PCJJG.
PhylomeDBiQ60829.
TreeFamiTF332576.

Enzyme and pathway databases

ReactomeiR-MMU-180024. DARPP-32 events.

Miscellaneous databases

PROiQ60829.
SOURCEiSearch...

Gene expression databases

BgeeiQ60829.
GenevisibleiQ60829. MM.

Family and domain databases

InterProiIPR015670. DARPP-32.
IPR008466. PPP1R1A/B/C.
[Graphical view]
PANTHERiPTHR15417. PTHR15417. 1 hit.
PTHR15417:SF2. PTHR15417:SF2. 1 hit.
PfamiPF05395. DARPP-32. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Liu Q.-R., Uhl G.R.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon and Salivary gland.
  4. "DARPP-32 promoter directs transgene expression to renal thick ascending limb of loop of Henle."
    Blau S., Daly L., Fienberg A., Teitelman G., Ehrlich M.E.
    Am. J. Physiol. 269:F564-F570(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
    Strain: BALB/cJ.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 48-194.
    Strain: C57BL/6J.
    Tissue: Hippocampus.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-46; THR-75; SER-97 AND SER-192, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Lung and Pancreas.

Entry informationi

Entry nameiPPR1B_MOUSE
AccessioniPrimary (citable) accession number: Q60829
Secondary accession number(s): A2A564
, Q3URF2, Q6DV86, Q91XB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 10, 2002
Last modified: July 6, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.