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Protein

Sodium-dependent phosphate transport protein 2A

Gene

Slc34a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane. Probably mediates 70-80% of the apical influx.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent phosphate transport protein 2A
Short name:
Sodium-phosphate transport protein 2A
Alternative name(s):
Na(+)-dependent phosphate cotransporter 2A
NaPi-7
Sodium/phosphate cotransporter 2A
Short name:
Na(+)/Pi cotransporter 2A
Short name:
NaPi-2a
Solute carrier family 34 member 1
Gene namesi
Name:Slc34a1
Synonyms:Npt2, Slc17a2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1345284. Slc34a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 103103CytoplasmicSequence analysisAdd
BLAST
Transmembranei104 – 12522Helical; Name=M1Sequence analysisAdd
BLAST
Topological domaini126 – 14520ExtracellularSequence analysisAdd
BLAST
Transmembranei146 – 16318Helical; Name=M2Sequence analysisAdd
BLAST
Topological domaini164 – 1652CytoplasmicSequence analysis
Transmembranei166 – 18520Helical; Name=M3Sequence analysisAdd
BLAST
Topological domaini186 – 345160ExtracellularSequence analysisAdd
BLAST
Transmembranei346 – 36823Helical; Name=M4Sequence analysisAdd
BLAST
Topological domaini369 – 41042CytoplasmicSequence analysisAdd
BLAST
Transmembranei411 – 43424Helical; Name=M5Sequence analysisAdd
BLAST
Topological domaini435 – 46430ExtracellularSequence analysisAdd
BLAST
Transmembranei465 – 48521Helical; Name=M6Sequence analysisAdd
BLAST
Topological domaini486 – 51126CytoplasmicSequence analysisAdd
BLAST
Transmembranei512 – 53221Helical; Name=M7Sequence analysisAdd
BLAST
Topological domaini533 – 5375ExtracellularSequence analysis
Transmembranei538 – 55922Helical; Name=M8Sequence analysisAdd
BLAST
Topological domaini560 – 63778CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • membrane-bounded vesicle Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637Sodium-dependent phosphate transport protein 2APRO_0000068608Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei34 – 341PhosphoserineCombined sources
Disulfide bondi225 ↔ 520By similarity
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence analysis
Disulfide bondi306 ↔ 334By similarity
Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence analysis
Modified residuei506 – 5061Phosphothreonine; by PKCSequence analysis
Modified residuei605 – 6051PhosphoserineBy similarity
Modified residuei621 – 6211PhosphothreonineCombined sources
Modified residuei623 – 6231PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ60825.
PaxDbiQ60825.
PRIDEiQ60825.

PTM databases

iPTMnetiQ60825.
PhosphoSiteiQ60825.

Expressioni

Tissue specificityi

Kidney.

Inductioni

Up-regulated by low-phosphate diet.1 Publication

Interactioni

Subunit structurei

Interacts via its C-terminal region with PDZK2. Interacts with SLC9A3R1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Pdzd3Q99MJ62EBI-7454990,EBI-7455069
Pdzk1Q9JIL42EBI-7454990,EBI-7420331
Slc9a3r1P704412EBI-7454990,EBI-1184085
Slc9a3r2Q9JHL12EBI-7454990,EBI-538451

Protein-protein interaction databases

IntActiQ60825. 11 interactions.
MINTiMINT-152214.
STRINGi10090.ENSMUSP00000059138.

Structurei

3D structure databases

ProteinModelPortaliQ60825.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SLC34A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE8P. Eukaryota.
COG1283. LUCA.
HOVERGENiHBG006527.
InParanoidiQ60825.

Family and domain databases

InterProiIPR003841. Na/Pi_transpt.
IPR029848. Na/Pi_transpt_2A.
[Graphical view]
PANTHERiPTHR10010:SF21. PTHR10010:SF21. 1 hit.
PfamiPF02690. Na_Pi_cotrans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01013. 2a58. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSYSERLGG PAVSPLPVRG RHMVHGATFA YVPSPQVLHR IPGTSTYAIS
60 70 80 90 100
SLSPVTLTEH SCPCGEVLEC HDPLPTKLAQ EEEQKPEPRL SQKLAQVGTK
110 120 130 140 150
LLKVPLMLAF LYLFVCSLDV LSSAFQLAGG KVAGDIFKDN AILSNPVAGL
160 170 180 190 200
VVGILVTVLV QSSSTSTSII VSMVSSGLLE VSSAIPIIMG SNIGTSVTNT
210 220 230 240 250
IVALMQAGDR TDFRRAFAGA TVHDCFNWLS VLVLLPLEAA TGYLHHVTGL
260 270 280 290 300
VVASFNIRGG RDAPDLLKVI TEPFTRLIIQ LDKSVITSIA VGDESLRNHS
310 320 330 340 350
LIRIWCHPDT TEASTSMSRV EAIGSLANTT MEKCNHIFVD TGLPDLAVGL
360 370 380 390 400
ILLAGSLVVL CTCLILLVKM LNSLLKGQVM SSRRSSTQTD FPAPFTWVTG
410 420 430 440 450
YFAMVVGASM TFVVQSSSVF TSAITPLIGL GVISIERAYP LTLGSNIGTT
460 470 480 490 500
TTAILAALAS PREKLSSSFQ IALCHFFFNI SGILLWYPLP CTRLPIRMAK
510 520 530 540 550
ALGKRTAKYR WFAVLYLLVC FLLLPSLVFG ISMAGWQAMV GVGTPFGALL
560 570 580 590 600
AFVVLVNVLQ SRSPGHLPKW LQTWDFLPRW MHSLQPLDGL ITRATLCYAR
610 620 630
PEPRSPQLPP RVFLEELPPA TPSPRLALPA HHNATRL
Length:637
Mass (Da):68,774
Last modified:July 27, 2011 - v2
Checksum:iEF6F68AB1EDC6557
GO

Sequence cautioni

The sequence AAC52684.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti324 – 3241G → A in AAC42026 (PubMed:8070635).Curated
Sequence conflicti345 – 3451D → A in AAC52361 (PubMed:7478940).Curated
Sequence conflicti379 – 3791V → VA in AAC42026 (PubMed:8070635).Curated
Sequence conflicti379 – 3791V → VA in AAC52361 (PubMed:7478940).Curated
Sequence conflicti379 – 3791V → VA in AAC52690 (PubMed:8693007).Curated
Sequence conflicti380 – 38910MSSRRSSTQT → NVIQKVINTD in AAC42026 (PubMed:8070635).Curated
Sequence conflicti390 – 3901Missing in AAC52361 (PubMed:7478940).Curated
Sequence conflicti390 – 3901Missing in AAC52690 (PubMed:8693007).Curated
Sequence conflicti444 – 4441G → D in AAC42026 (PubMed:8070635).Curated
Sequence conflicti458 – 4581L → V in AAC42026 (PubMed:8070635).Curated
Sequence conflicti458 – 4581L → V in AAC52361 (PubMed:7478940).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33878 mRNA. Translation: AAC42026.1.
U22465 mRNA. Translation: AAC52361.1.
CT009762 Genomic DNA. No translation available.
U56664 Genomic DNA. Translation: AAC52684.1. Different initiation.
U56665 Genomic DNA. Translation: AAC52685.1.
U56666 Genomic DNA. Translation: AAC52686.1.
U56667 Genomic DNA. Translation: AAC52687.1.
U56668 Genomic DNA. Translation: AAC52688.1.
U56669 Genomic DNA. No translation available.
U56670 Genomic DNA. Translation: AAC52689.1.
U56671 Genomic DNA. Translation: AAC52690.1.
U56672 Genomic DNA. Translation: AAC52691.1.
U56673 Genomic DNA. Translation: AAC52692.1.
CCDSiCCDS49271.1.
UniGeneiMm.276355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33878 mRNA. Translation: AAC42026.1.
U22465 mRNA. Translation: AAC52361.1.
CT009762 Genomic DNA. No translation available.
U56664 Genomic DNA. Translation: AAC52684.1. Different initiation.
U56665 Genomic DNA. Translation: AAC52685.1.
U56666 Genomic DNA. Translation: AAC52686.1.
U56667 Genomic DNA. Translation: AAC52687.1.
U56668 Genomic DNA. Translation: AAC52688.1.
U56669 Genomic DNA. No translation available.
U56670 Genomic DNA. Translation: AAC52689.1.
U56671 Genomic DNA. Translation: AAC52690.1.
U56672 Genomic DNA. Translation: AAC52691.1.
U56673 Genomic DNA. Translation: AAC52692.1.
CCDSiCCDS49271.1.
UniGeneiMm.276355.

3D structure databases

ProteinModelPortaliQ60825.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ60825. 11 interactions.
MINTiMINT-152214.
STRINGi10090.ENSMUSP00000059138.

PTM databases

iPTMnetiQ60825.
PhosphoSiteiQ60825.

Proteomic databases

MaxQBiQ60825.
PaxDbiQ60825.
PRIDEiQ60825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1345284. Slc34a1.

Phylogenomic databases

eggNOGiENOG410IE8P. Eukaryota.
COG1283. LUCA.
HOVERGENiHBG006527.
InParanoidiQ60825.

Miscellaneous databases

ChiTaRSiSlc34a1. mouse.
PROiQ60825.
SOURCEiSearch...

Family and domain databases

InterProiIPR003841. Na/Pi_transpt.
IPR029848. Na/Pi_transpt_2A.
[Graphical view]
PANTHERiPTHR10010:SF21. PTHR10010:SF21. 1 hit.
PfamiPF02690. Na_Pi_cotrans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01013. 2a58. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, functional expression, tissue distribution, and in situ hybridization of the renal sodium phosphate (Na+/P(i)) transporter in the control and hypophosphatemic mouse."
    Collins J.F., Ghishan F.K.
    FASEB J. 8:862-869(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney cortex.
  2. "Transport characteristics of a murine renal Na/Pi-cotransporter."
    Hartmann C.M., Wagner C.A., Busch A.E., Markovich D., Biber J., Lang F., Murer H.
    Pflugers Arch. 430:830-836(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Kidney cortex.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Structure of murine and human renal type II Na+-phosphate cotransporter genes (Npt2 and NPT2)."
    Hartmann C.M., Hewson A.S., Kos C.H., Hilfiker H., Soumounou Y., Murer H., Tenenhouse H.S.
    Proc. Natl. Acad. Sci. U.S.A. 93:7409-7414(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-94; 110-177; 179-242; 254-312; 313-318; 332-333; 335-337 AND 349-485.
    Strain: 129/Sv.
  5. "Identification of regulatory sequences and binding proteins in the type II sodium/phosphate cotransporter NPT2 gene responsive to dietary phosphate."
    Kido S., Miyamoto K., Mizobuchi H., Taketani Y., Ohkido I., Ogawa N., Kaneko Y., Harashima S., Takeda E.
    J. Biol. Chem. 274:28256-28263(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: ICR.
  6. "A novel PDZ protein regulates the activity of guanylyl cyclase C, the heat-stable enterotoxin receptor."
    Scott R.O., Thelin W.R., Milgram S.L.
    J. Biol. Chem. 277:22934-22941(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PDZK2.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; THR-621 AND SER-623, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney.

Entry informationi

Entry nameiNPT2A_MOUSE
AccessioniPrimary (citable) accession number: Q60825
Secondary accession number(s): E9QKA0
, Q62110, Q62111, Q62112, Q62113, Q62114, Q62115, Q62116, Q62564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: April 13, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.