Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q60821

- ZBT17_MOUSE

UniProt

Q60821 - ZBT17_MOUSE

Protein

Zinc finger and BTB domain-containing protein 17

Gene

Zbtb17

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation. Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri297 – 31923C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri325 – 34723C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri353 – 37523C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri381 – 40323C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri409 – 43123C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri437 – 45923C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri465 – 48723C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri493 – 51523C2H2-type 8PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri519 – 54325C2H2-type 9PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri549 – 57123C2H2-type 10PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri577 – 59923C2H2-type 11PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri605 – 62824C2H2-type 12PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri708 – 73023C2H2-type 13PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding transcription factor activity Source: Ensembl

    GO - Biological processi

    1. ectoderm development Source: MGI
    2. gastrulation with mouth forming second Source: MGI
    3. positive regulation of transcription, DNA-templated Source: MGI
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_106572. XBP1(S) activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger and BTB domain-containing protein 17
    Alternative name(s):
    LP-1
    Polyomavirus late initiator promoter-binding protein
    Zinc finger protein 100
    Short name:
    Zfp-100
    Zinc finger protein 151
    Zinc finger protein Z13
    Gene namesi
    Name:Zbtb17
    Synonyms:Zfp100, Znf151
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:107410. Zbtb17.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice produce inviable embryos which are severely retarded in early development and do not undergo normal gastrulation due to massive apoptosis of ectodermal cells around day 7.5.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 794794Zinc finger and BTB domain-containing protein 17PRO_0000047731Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki388 – 388Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki472 – 472Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Post-translational modificationi

    Undergoes 'Lys-48'-linked polyubiquitination at Lys-388 and Lys-472 and subsequent proteasomal degradation in a TRAF2-dependent manner and upon TNFA stimulation.1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiQ60821.
    PRIDEiQ60821.

    PTM databases

    PhosphoSiteiQ60821.

    Expressioni

    Tissue specificityi

    Found in all the embryonic and adult tissues examined.

    Developmental stagei

    Expressed ubiquitously from early embryogenesis (6.5 dpc) to organogenesis, predominantly in neural and epithelial tissues.1 Publication

    Gene expression databases

    BgeeiQ60821.
    CleanExiMM_ZBTB17.
    GenevestigatoriQ60821.

    Interactioni

    Subunit structurei

    Homooligomerizes (via the BTB/POZ domain), multimerization is required for DNA binding. Binds to the C-terminal helix-loop-helix motif of MYC which inhibits ZBTB17 transactivation and growth arrest activities and renders it insoluble in the nucleus. Also interacts with HCFC1, MAGEA4 and TMPRSS11A. Interacts (via the C-terminal zinc fingers) with GFI1; the interaction results in the recruitment of MYC to the CDKN1A/p21 and CDKN1B promoters and repression of transcription. Interacts with TRAF2, interfering with the binding of UBC13 to TRAF2, and inhibiting TRAF2 E3 ligase activity. Interacts with BCL6; the interaction inhibits ZBTB17 transactivation activity on target genes involved in cell cycle arrest.2 Publications

    Protein-protein interaction databases

    BioGridi204631. 2 interactions.
    DIPiDIP-48690N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60821.
    SMRiQ60821. Positions 1-115, 297-626, 698-740.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 104104BTBPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni260 – 29940Interaction with MYCBy similarityAdd
    BLAST
    Regioni628 – 794167Interaction with HCFC1By similarityAdd
    BLAST
    Regioni628 – 70982Interaction with MYCBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
    Contains 13 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri297 – 31923C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri325 – 34723C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri353 – 37523C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri381 – 40323C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri409 – 43123C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri437 – 45923C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri465 – 48723C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri493 – 51523C2H2-type 8PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri519 – 54325C2H2-type 9PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri549 – 57123C2H2-type 10PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri577 – 59923C2H2-type 11PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri605 – 62824C2H2-type 12PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri708 – 73023C2H2-type 13PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00750000117570.
    HOGENOMiHOG000231298.
    HOVERGENiHBG054919.
    InParanoidiA2ADB9.
    KOiK10500.
    OMAiCDSCGDK.
    OrthoDBiEOG7KSX7Q.
    TreeFamiTF332047.

    Family and domain databases

    Gene3Di3.30.160.60. 13 hits.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00651. BTB. 1 hit.
    PF00096. zf-C2H2. 1 hit.
    [Graphical view]
    SMARTiSM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 13 hits.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 13 hits.
    PS50157. ZINC_FINGER_C2H2_2. 13 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60821-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFPQHSQRV LEQLNQQRQL GLLCDCTFVV DGVDFKAHKA VLAACSEYFK    50
    MLFVDQKDVV HLDISNAAGL GQVLEFMYTA KLSLSPENVD DVLAVASFLQ 100
    MQDIVTACHT LKSLAEPSST TGESADASAV EGGDKRAKDE KAAATMLSRL 150
    DQARGSSSTG PGRELKEERG GQAESASSGA EQTEKADAPR EPPPVELKPD 200
    PTSSMAAAEA EALSESSEQE MEVEPASKGE DGQEEEGAGP ATVKEEGMHL 250
    DNGEPPEENE ESAGTDSGQE LGMEGQNLRS GTYGDRTESK AYGSIIHKCE 300
    DCGKEFTHTG NFKRHIRIHT GEKPFSCREC SKAFSDPAAC KAHEKTHSPL 350
    KPYGCEECGK SYRLISLLNL HKKRHSGEAR YRCGDCGKLF TTSGNLKRHQ 400
    LVHSGQKPYQ CDYCGRSFSD PTSKMRHLET HDTDKEHKCP HCDKKFNQVG 450
    NLKAHLKIHI ADGPLKCREC GKQFTTSGNL KRHLRIHSGE KPYVCTHCQR 500
    QFADPGALQR HVRIHTGEKP CQCVICGKAF TQASSLIAHV RQHTGEKPYV 550
    CERCGKRFVQ SSQLANHIRH HDNIRPHKCS VCSKAFVNVG DLSKHIIIHT 600
    GEKPYLCDKC GRGFNRVDNL RSHVKTVHQG KAGIKILEPE EGGEVSVVTV 650
    DDMVTLATEA LAATAVTQLT VVPVGAAVTA DETEVLKAEI SKAVKQVQEE 700
    DPNTHILYAC DSCGDKFLDA NSLAQHVRIH TAQALVMFQT DADFYQQYGP 750
    GSTWPAGQML QAGELVFRPR DGTEGQPTLA ESPPTAPDCL PPAE 794
    Length:794
    Mass (Da):86,758
    Last modified:July 27, 2011 - v2
    Checksum:i54CF6CF0D2C60303
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti151 – 1511D → G in AAA64848. 1 PublicationCurated
    Sequence conflicti151 – 1511D → G in AAA85493. (PubMed:7575457)Curated
    Sequence conflicti507 – 5071A → G in AAA64848. 1 PublicationCurated
    Sequence conflicti573 – 5731N → K in AAA85493. (PubMed:7575457)Curated
    Sequence conflicti577 – 5771H → D in AAA64848. 1 PublicationCurated
    Sequence conflicti577 – 5771H → D in AAA85493. (PubMed:7575457)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22396 mRNA. Translation: AAA64848.1.
    U14556 mRNA. Translation: AAA85493.1.
    AL670285 Genomic DNA. Translation: CAM20401.1.
    CH466615 Genomic DNA. Translation: EDL13378.1.
    CCDSiCCDS18875.1.
    PIRiS59069.
    RefSeqiNP_033567.2. NM_009541.2.
    UniGeneiMm.389590.

    Genome annotation databases

    EnsembliENSMUST00000006377; ENSMUSP00000006377; ENSMUSG00000006215.
    GeneIDi22642.
    KEGGimmu:22642.
    UCSCiuc008vol.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22396 mRNA. Translation: AAA64848.1 .
    U14556 mRNA. Translation: AAA85493.1 .
    AL670285 Genomic DNA. Translation: CAM20401.1 .
    CH466615 Genomic DNA. Translation: EDL13378.1 .
    CCDSi CCDS18875.1.
    PIRi S59069.
    RefSeqi NP_033567.2. NM_009541.2.
    UniGenei Mm.389590.

    3D structure databases

    ProteinModelPortali Q60821.
    SMRi Q60821. Positions 1-115, 297-626, 698-740.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204631. 2 interactions.
    DIPi DIP-48690N.

    PTM databases

    PhosphoSitei Q60821.

    Proteomic databases

    PaxDbi Q60821.
    PRIDEi Q60821.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006377 ; ENSMUSP00000006377 ; ENSMUSG00000006215 .
    GeneIDi 22642.
    KEGGi mmu:22642.
    UCSCi uc008vol.2. mouse.

    Organism-specific databases

    CTDi 7709.
    MGIi MGI:107410. Zbtb17.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00750000117570.
    HOGENOMi HOG000231298.
    HOVERGENi HBG054919.
    InParanoidi A2ADB9.
    KOi K10500.
    OMAi CDSCGDK.
    OrthoDBi EOG7KSX7Q.
    TreeFami TF332047.

    Enzyme and pathway databases

    Reactomei REACT_106572. XBP1(S) activates chaperone genes.

    Miscellaneous databases

    NextBioi 303035.
    PROi Q60821.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q60821.
    CleanExi MM_ZBTB17.
    Genevestigatori Q60821.

    Family and domain databases

    Gene3Di 3.30.160.60. 13 hits.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00651. BTB. 1 hit.
    PF00096. zf-C2H2. 1 hit.
    [Graphical view ]
    SMARTi SM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 13 hits.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 13 hits.
    PS50157. ZINC_FINGER_C2H2_2. 13 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA for polyomavirus late initiator promoter binding protein, LP-1."
      Rapp L., Carmichael G.G.
      Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "An unusual arrangement of 13 zinc fingers in the vertebrate gene Z13."
      Schulz T.C., Hopwood B., Rathjen P.D., Wells J.R.E.
      Biochem. J. 311:219-224(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CBA.
      Tissue: Kidney.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Miz1 is required for early embryonic development during gastrulation."
      Adhikary S., Peukert K., Karsunky H., Beuger V., Lutz W., Elsaesser H.-P., Moeroey T., Eilers M.
      Mol. Cell. Biol. 23:7648-7657(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    6. "A role of Miz-1 in Gfi-1-mediated transcriptional repression of CDKN1A."
      Liu Q., Basu S., Qiu Y., Tang F., Dong F.
      Oncogene 29:2843-2852(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GFI1, FUNCTION.
    7. "IL-7R-dependent survival and differentiation of early T-lineage progenitors is regulated by the BTB/POZ domain transcription factor Miz-1."
      Saba I., Kosan C., Vassen L., Moroy T.
      Blood 117:3370-3381(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN LYMPHOCYTE DEVELOPMENT.
    8. "Site-specific ubiquitination is required for relieving the transcription factor Miz1-mediated suppression on TNF-alpha-induced JNK activation and inflammation."
      Liu J., Yan J., Jiang S., Wen J., Chen L., Zhao Y., Lin A.
      Proc. Natl. Acad. Sci. U.S.A. 109:191-196(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION AT LYS-388 AND LYS-472, INTERACTION WITH TRAF2.

    Entry informationi

    Entry nameiZBT17_MOUSE
    AccessioniPrimary (citable) accession number: Q60821
    Secondary accession number(s): A2ADB9, Q60699
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3