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Q60821 (ZBT17_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger and BTB domain-containing protein 17
Alternative name(s):
LP-1
Polyomavirus late initiator promoter-binding protein
Zinc finger protein 100
Short name=Zfp-100
Zinc finger protein 151
Zinc finger protein Z13
Gene names
Name:Zbtb17
Synonyms:Zfp100, Znf151
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length794 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation. Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment. Ref.5 Ref.6 Ref.7

Subunit structure

Homooligomerizes (via the BTB/POZ domain), multimerization is required for DNA binding. Binds to the C-terminal helix-loop-helix motif of MYC which inhibits ZBTB17 transactivation and growth arrest activities and renders it insoluble in the nucleus. Also interacts with HCFC1, MAGEA4 and TMPRSS11A. Interacts (via the C-terminal zinc fingers) with GFI1; the interaction results in the recruitment of MYC to the CDKN1A/p21 and CDKN1B promoters and repression of transcription. Interacts with TRAF2, interfering with the binding of UBC13 to TRAF2, and inhibiting TRAF2 E3 ligase activity. Interacts with BCL6; the interaction inhibits ZBTB17 transactivation activity on target genes involved in cell cycle arrest. Ref.6 Ref.8

Subcellular location

Nucleus By similarity.

Tissue specificity

Found in all the embryonic and adult tissues examined.

Developmental stage

Expressed ubiquitously from early embryogenesis (6.5 dpc) to organogenesis, predominantly in neural and epithelial tissues. Ref.5

Post-translational modification

Undergoes 'Lys-48'-linked polyubiquitination at Lys-388 and Lys-472 and subsequent proteasomal degradation in a TRAF2-dependent manner and upon TNFA stimulation.

Disruption phenotype

Mice produce inviable embryos which are severely retarded in early development and do not undergo normal gastrulation due to massive apoptosis of ectodermal cells around day 7.5. Ref.5

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 1 BTB (POZ) domain.

Contains 13 C2H2-type zinc fingers.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 794794Zinc finger and BTB domain-containing protein 17
PRO_0000047731

Regions

Domain1 – 104104BTB
Zinc finger297 – 31923C2H2-type 1
Zinc finger325 – 34723C2H2-type 2
Zinc finger353 – 37523C2H2-type 3
Zinc finger381 – 40323C2H2-type 4
Zinc finger409 – 43123C2H2-type 5
Zinc finger437 – 45923C2H2-type 6
Zinc finger465 – 48723C2H2-type 7
Zinc finger493 – 51523C2H2-type 8
Zinc finger519 – 54325C2H2-type 9
Zinc finger549 – 57123C2H2-type 10
Zinc finger577 – 59923C2H2-type 11
Zinc finger605 – 62824C2H2-type 12
Zinc finger708 – 73023C2H2-type 13
Region260 – 29940Interaction with MYC By similarity
Region628 – 794167Interaction with HCFC1 By similarity
Region628 – 70982Interaction with MYC By similarity

Amino acid modifications

Cross-link388Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.8
Cross-link472Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.8

Experimental info

Sequence conflict1511D → G in AAA64848. Ref.1
Sequence conflict1511D → G in AAA85493. Ref.2
Sequence conflict5071A → G in AAA64848. Ref.1
Sequence conflict5731N → K in AAA85493. Ref.2
Sequence conflict5771H → D in AAA64848. Ref.1
Sequence conflict5771H → D in AAA85493. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q60821 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 54CF6CF0D2C60303

FASTA79486,758
        10         20         30         40         50         60 
MDFPQHSQRV LEQLNQQRQL GLLCDCTFVV DGVDFKAHKA VLAACSEYFK MLFVDQKDVV 

        70         80         90        100        110        120 
HLDISNAAGL GQVLEFMYTA KLSLSPENVD DVLAVASFLQ MQDIVTACHT LKSLAEPSST 

       130        140        150        160        170        180 
TGESADASAV EGGDKRAKDE KAAATMLSRL DQARGSSSTG PGRELKEERG GQAESASSGA 

       190        200        210        220        230        240 
EQTEKADAPR EPPPVELKPD PTSSMAAAEA EALSESSEQE MEVEPASKGE DGQEEEGAGP 

       250        260        270        280        290        300 
ATVKEEGMHL DNGEPPEENE ESAGTDSGQE LGMEGQNLRS GTYGDRTESK AYGSIIHKCE 

       310        320        330        340        350        360 
DCGKEFTHTG NFKRHIRIHT GEKPFSCREC SKAFSDPAAC KAHEKTHSPL KPYGCEECGK 

       370        380        390        400        410        420 
SYRLISLLNL HKKRHSGEAR YRCGDCGKLF TTSGNLKRHQ LVHSGQKPYQ CDYCGRSFSD 

       430        440        450        460        470        480 
PTSKMRHLET HDTDKEHKCP HCDKKFNQVG NLKAHLKIHI ADGPLKCREC GKQFTTSGNL 

       490        500        510        520        530        540 
KRHLRIHSGE KPYVCTHCQR QFADPGALQR HVRIHTGEKP CQCVICGKAF TQASSLIAHV 

       550        560        570        580        590        600 
RQHTGEKPYV CERCGKRFVQ SSQLANHIRH HDNIRPHKCS VCSKAFVNVG DLSKHIIIHT 

       610        620        630        640        650        660 
GEKPYLCDKC GRGFNRVDNL RSHVKTVHQG KAGIKILEPE EGGEVSVVTV DDMVTLATEA 

       670        680        690        700        710        720 
LAATAVTQLT VVPVGAAVTA DETEVLKAEI SKAVKQVQEE DPNTHILYAC DSCGDKFLDA 

       730        740        750        760        770        780 
NSLAQHVRIH TAQALVMFQT DADFYQQYGP GSTWPAGQML QAGELVFRPR DGTEGQPTLA 

       790 
ESPPTAPDCL PPAE 

« Hide

References

« Hide 'large scale' references
[1]"cDNA for polyomavirus late initiator promoter binding protein, LP-1."
Rapp L., Carmichael G.G.
Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"An unusual arrangement of 13 zinc fingers in the vertebrate gene Z13."
Schulz T.C., Hopwood B., Rathjen P.D., Wells J.R.E.
Biochem. J. 311:219-224(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CBA.
Tissue: Kidney.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Miz1 is required for early embryonic development during gastrulation."
Adhikary S., Peukert K., Karsunky H., Beuger V., Lutz W., Elsaesser H.-P., Moeroey T., Eilers M.
Mol. Cell. Biol. 23:7648-7657(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[6]"A role of Miz-1 in Gfi-1-mediated transcriptional repression of CDKN1A."
Liu Q., Basu S., Qiu Y., Tang F., Dong F.
Oncogene 29:2843-2852(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GFI1, FUNCTION.
[7]"IL-7R-dependent survival and differentiation of early T-lineage progenitors is regulated by the BTB/POZ domain transcription factor Miz-1."
Saba I., Kosan C., Vassen L., Moroy T.
Blood 117:3370-3381(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN LYMPHOCYTE DEVELOPMENT.
[8]"Site-specific ubiquitination is required for relieving the transcription factor Miz1-mediated suppression on TNF-alpha-induced JNK activation and inflammation."
Liu J., Yan J., Jiang S., Wen J., Chen L., Zhao Y., Lin A.
Proc. Natl. Acad. Sci. U.S.A. 109:191-196(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION AT LYS-388 AND LYS-472, INTERACTION WITH TRAF2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U22396 mRNA. Translation: AAA64848.1.
U14556 mRNA. Translation: AAA85493.1.
AL670285 Genomic DNA. Translation: CAM20401.1.
CH466615 Genomic DNA. Translation: EDL13378.1.
CCDSCCDS18875.1.
PIRS59069.
RefSeqNP_033567.2. NM_009541.2.
UniGeneMm.389590.

3D structure databases

ProteinModelPortalQ60821.
SMRQ60821. Positions 1-115, 297-626, 698-740.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204631. 2 interactions.
DIPDIP-48690N.

PTM databases

PhosphoSiteQ60821.

Proteomic databases

PaxDbQ60821.
PRIDEQ60821.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006377; ENSMUSP00000006377; ENSMUSG00000006215.
GeneID22642.
KEGGmmu:22642.
UCSCuc008vol.2. mouse.

Organism-specific databases

CTD7709.
MGIMGI:107410. Zbtb17.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00750000117570.
HOGENOMHOG000231298.
HOVERGENHBG054919.
InParanoidA2ADB9.
KOK10500.
OMACDSCGDK.
OrthoDBEOG7KSX7Q.
TreeFamTF332047.

Gene expression databases

BgeeQ60821.
CleanExMM_ZBTB17.
GenevestigatorQ60821.

Family and domain databases

Gene3D3.30.160.60. 13 hits.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00651. BTB. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 13 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 13 hits.
PS50157. ZINC_FINGER_C2H2_2. 13 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio303035.
PROQ60821.
SOURCESearch...

Entry information

Entry nameZBT17_MOUSE
AccessionPrimary (citable) accession number: Q60821
Secondary accession number(s): A2ADB9, Q60699
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot