Q60819 (I15RA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-15 receptor subunit alpha
Short name=IL-15 receptor subunit alpha
Short name=IL-15R-alpha
Short name=IL-15RA

Alternative name(s):
CD_antigen=CD215

Cleaved into the following chain:

1. Soluble interleukin-15 receptor subunit alpha
   Short name=sIL-15 receptor subunit alpha
   Short name=sIL-15R-alpha
   Short name=sIL-15RA

Gene names

Name:

Il15ra

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Signal transduction seems to involve STAT3, STAT5, STAT6, JAK2 and SYK. Expression of different isoforms may alter or interfere with signal transduction. Ref.5 Ref.7
Subunit structure	The interleukin-15 receptor IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL15RA also self-associates. Interacts with SYK. Ref.1 Ref.5
Subcellular location	Membrane; Single-pass type I membrane protein Ref.5. Isoform 1: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein Ref.5. Isoform 3: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein Ref.5. Isoform 4: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein Ref.5. Isoform 6: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein Ref.5. Soluble interleukin-15 receptor subunit alpha: Secreted › extracellular space By similarity Ref.5.
Tissue specificity	Widely expressed. Isoform 2, isoform 3 and isoform 4 are expressed in natural killer (NK) cells and T-cells. Isoform 5 is also expressed in NK cells. Isoform 3, isoform 4 and isoform 6 but not isoform 1 are expressed in mast cells. Ref.1 Ref.2
Post-translational modification	Phosphorylated by activated SYK By similarity. Isoform 3, isoform 4 and isoform 6 are N-glycosylated but not O-glycosylated. Ref.5 A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE. It also binds IL15 and thus interferes with IL15 binding to the membrane receptor By similarity.
Sequence similarities	Contains 1 Sushi (CCP/SCR) domain.

Ontologies

Keywords
Cellular component	Cell membrane Endoplasmic reticulum Golgi apparatus Membrane Nucleus Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal Sushi Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of natural killer cell differentiation Inferred from mutant phenotype. Source: MGI
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q60819-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q60819-2)

Also known as: 1A;

The sequence of this isoform differs from the canonical sequence as follows:
     129-140: Missing.
     141-161: Missing.

Isoform 3 (identifier: Q60819-3)

Also known as: 1B; IL-15R-alphadelta4;

The sequence of this isoform differs from the canonical sequence as follows:
     129-140: Missing.
     141-161: Missing.
     162-194: Missing.

Isoform 4 (identifier: Q60819-4)

Also known as: 1C; IL-15R-alphadelta34;

The sequence of this isoform differs from the canonical sequence as follows:
     98-128: Missing.
     129-140: Missing.
     141-161: Missing.
     162-194: Missing.
     195-195: E → K

Isoform 5 (identifier: Q60819-5)

Also known as: 2; 2A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: Missing.
     98-128: Missing.
     129-140: Missing.

Isoform 6 (identifier: Q60819-6)

Also known as: IL-15R-alphadelta345;

The sequence of this isoform differs from the canonical sequence as follows:
     98-128: Missing.
     129-140: Missing.
     141-161: Missing.
     162-194: Missing.
     195-206: EISPHSSKMTKV → M

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

32

Potential

Chain

33 – 263

231

Interleukin-15 receptor subunit alpha

PRO_0000011045

Chain

33 – ?

Soluble interleukin-15 receptor subunit alpha

PRO_0000333856

Regions

Topological domain

33 – 205

173

Extracellular Potential

Transmembrane

206 – 226

21

Helical; Potential

Topological domain

227 – 263

37

Cytoplasmic Potential

Domain

34 – 98

65

Sushi

Amino acid modifications

Glycosylation

51

1

N-linked (GlcNAc...) Potential

Disulfide bond

36 ↔ 78

Ref.7

Disulfide bond

62 ↔ 96

Ref.7

Natural variations

Alternative sequence

1 – 97

97

Missing in isoform 5.

VSP_012627

Alternative sequence

98 – 128

31

Missing in isoform 4, isoform 5 and isoform 6.

VSP_012628

Alternative sequence

129 – 140

12

Missing in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6.

VSP_012629

Alternative sequence

141 – 161

21

Missing in isoform 2, isoform 3, isoform 4 and isoform 6.

VSP_012630

Alternative sequence

162 – 194

33

Missing in isoform 3, isoform 4 and isoform 6.

VSP_012631

Alternative sequence

195 – 206

12

EISPH…KMTKV → M in isoform 6.

VSP_012633

Alternative sequence

195

1

E → K in isoform 4.

VSP_012632

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

263

Helix Strand Turn

Details...

Beta strand

57 – 59

3

Beta strand

66 – 68

3

Beta strand

75 – 80

6

Turn

82 – 84

3

Beta strand

87 – 89

3

Beta strand

96 – 98

3

Turn

100 – 102

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BFCC2CE4BA58B504	FASTA	263	28,061
	10 20 30 40 50 60 MASPQLRGYG VQAIPVLLLL LLLLLLPLRV TPGTTCPPPV SIEHADIRVK NYSVNSRERY 70 80 90 100 110 120 VCNSGFKRKA GTSTLIECVI NKNTNVAHWT TPSLKCIRDP SLAHYSPVPT VVTPKVTSQP 130 140 150 160 170 180 ESPSPSAKEP EAFSPKSDTA MTTETAIMPG SRLTPSQTTS AGTTGTGSHK SSRAPSLAAT 190 200 210 220 230 240 MTLEPTASTS LRITEISPHS SKMTKVAIST SVLLVGAGVV MAFLAWYIKS RQPSQPCRVE 250 260 VETMETVPMT VRASSKEDED TGA
	Isoform 2 (1A) [UniParc]. Checksum: 23CF1CE4DA3F70F1	FASTA	230	24,638
	10 20 30 40 50 60 MASPQLRGYG VQAIPVLLLL LLLLLLPLRV TPGTTCPPPV SIEHADIRVK NYSVNSRERY 70 80 90 100 110 120 VCNSGFKRKA GTSTLIECVI NKNTNVAHWT TPSLKCIRDP SLAHYSPVPT VVTPKVTSQP 130 140 150 160 170 180 ESPSPSAKGT TGTGSHKSSR APSLAATMTL EPTASTSLRI TEISPHSSKM TKVAISTSVL 190 200 210 220 230 LVGAGVVMAF LAWYIKSRQP SQPCRVEVET METVPMTVRA SSKEDEDTGA
	Isoform 3 (1B) (IL-15R-alphadelta4) [UniParc]. Checksum: 1D16A75C518B986B	FASTA	197	21,366
	10 20 30 40 50 60 MASPQLRGYG VQAIPVLLLL LLLLLLPLRV TPGTTCPPPV SIEHADIRVK NYSVNSRERY 70 80 90 100 110 120 VCNSGFKRKA GTSTLIECVI NKNTNVAHWT TPSLKCIRDP SLAHYSPVPT VVTPKVTSQP 130 140 150 160 170 180 ESPSPSAKEI SPHSSKMTKV AISTSVLLVG AGVVMAFLAW YIKSRQPSQP CRVEVETMET 190 VPMTVRASSK EDEDTGA
	Isoform 4 (1C) (IL-15R-alphadelta34) [UniParc]. Checksum: 94DC3A60B7CD883B	FASTA	166	18,122
	10 20 30 40 50 60 MASPQLRGYG VQAIPVLLLL LLLLLLPLRV TPGTTCPPPV SIEHADIRVK NYSVNSRERY 70 80 90 100 110 120 VCNSGFKRKA GTSTLIECVI NKNTNVAHWT TPSLKCIKIS PHSSKMTKVA ISTSVLLVGA 130 140 150 160 GVVMAFLAWY IKSRQPSQPC RVEVETMETV PMTVRASSKE DEDTGA
	Isoform 5 (2) (2A) [UniParc]. Checksum: B43C11755D9823DC	FASTA	123	12,905
	10 20 30 40 50 60 MTTETAIMPG SRLTPSQTTS AGTTGTGSHK SSRAPSLAAT MTLEPTASTS LRITEISPHS 70 80 90 100 110 120 SKMTKVAIST SVLLVGAGVV MAFLAWYIKS RQPSQPCRVE VETMETVPMT VRASSKEDED TGA
	Isoform 6 (IL-15R-alphadelta345) [UniParc]. Checksum: B74A423264627E66	FASTA	155	16,929
	10 20 30 40 50 60 MASPQLRGYG VQAIPVLLLL LLLLLLPLRV TPGTTCPPPV SIEHADIRVK NYSVNSRERY 70 80 90 100 110 120 VCNSGFKRKA GTSTLIECVI NKNTNVAHWT TPSLKCIMAI STSVLLVGAG VVMAFLAWYI 130 140 150 KSRQPSQPCR VEVETMETVP MTVRASSKED EDTGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and cloning of a novel IL-15 binding protein that is structurally related to the alpha chain of the IL-2 receptor." Giri J.G., Kumaki S., Ahdieh M., Friend D.J., Loomis A., Shanebeck K., DuBose R., Cosman D., Park L.S., Anderson D.M. EMBO J. 14:3654-3663(1995) [PubMed: 7641685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), LIGAND-BINDING, SUBUNIT, TISSUE SPECIFICITY.
[2]	"Cytokine requirements for the growth and development of mouse NK cells in vitro." Toomey J.A., Gays F., Foster D., Brooks C.G. J. Leukoc. Biol. 74:233-242(2003) [PubMed: 12885940] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3; 4 AND 5), TISSUE SPECIFICITY. Strain: C57BL/6.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Strain: FVB/N. Tissue: Mammary tumor.
[5]	"Mast cells express novel functional IL-15 receptor alpha isoforms." Bulanova E., Budagian V., Orinska Z., Krause H., Paus R., Bulfone-Paus S. J. Immunol. 170:5045-5055(2003) [PubMed: 12734349] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 3; 4 AND 6), FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH SYK.
[6]	"Natural soluble interleukin-15Ralpha is generated by cleavage that involves the tumor necrosis factor-alpha-converting enzyme (TACE/ADAM17)." Budagian V., Bulanova E., Orinska Z., Ludwig A., Rose-John S., Saftig P., Borden E.C., Bulfone-Paus S. J. Biol. Chem. 279:40368-40375(2004) [PubMed: 15215246] [Abstract] Cited for: PROTEOLYTIC PROCESSING BY ADAM17.
[7]	"Crystal Structure of the interleukin-15.interleukin-15 receptor alpha complex: insights into trans and cis presentation." Olsen S.K., Ota N., Kishishita S., Kukimoto-Niino M., Murayama K., Uchiyama H., Toyama M., Terada T., Shirouzu M., Kanagawa O., Yokoyama S. J. Biol. Chem. 282:37191-37204(2007) [PubMed: 17947230] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.19 ANGSTROMS) OF 33-103 IN COMPLEX WITH IL15, FUNCTION, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U22339 Genomic DNA. Translation: AAC52240.1.
AY219715 mRNA. Translation: AAO62310.1.
AY219716 mRNA. Translation: AAO62311.1.
AY219717 mRNA. Translation: AAO62312.1.
AY221616 mRNA. Translation: AAO74882.1.
AL831794 Genomic DNA. Translation: CAM13743.1.
AL831794 Genomic DNA. Translation: CAM13744.1.
AL831794 Genomic DNA. Translation: CAM13745.1.
BC022705 mRNA. Translation: AAH22705.1.

IPI

IPI00111244.
IPI00121306.
IPI00403949.
IPI00458151.
IPI00551074.
IPI00551117.

PIR

S57346.

RefSeq

NP_032384.1. NM_008358.1.
NP_598597.1. NM_133836.1.

UniGene

Mm.200196.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PSM	X-ray	2.19	C/F	33-103	[»]

ProteinModelPortal

Q60819.

SMR

Q60819. Positions 35-103.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q60819. 1 interaction.

STRING

Q60819.

Proteomic databases

PRIDE

Q60819.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000078834; ENSMUSP00000077878; ENSMUSG00000023206.
ENSMUST00000114831; ENSMUSP00000110480; ENSMUSG00000023206.
ENSMUST00000114832; ENSMUSP00000110481; ENSMUSG00000023206.
ENSMUST00000114833; ENSMUSP00000110482; ENSMUSG00000023206.
ENSMUST00000114834; ENSMUSP00000110483; ENSMUSG00000023206.
ENSMUST00000128156; ENSMUSP00000126364; ENSMUSG00000023206.
ENSMUST00000138349; ENSMUSP00000131473; ENSMUSG00000023206.

GeneID

16169.

KEGG

mmu:16169.

UCSC

uc008iiw.1. mouse.
uc008iix.1. mouse.
uc008iiy.1. mouse.
uc008iiz.1. mouse.
uc008ija.1. mouse.

Organism-specific databases

CTD

3601.

MGI

MGI:104644. Il15ra.

Phylogenomic databases

GeneTree

ENSGT00390000000121.

HOGENOM

HBG125999.

HOVERGEN

HBG052061.

InParanoid

Q60819.

OMA

DLENCSH.

OrthoDB

EOG4GXFNZ.

PhylomeDB

Q60819.

Gene expression databases

ArrayExpress

Q60819.

Bgee

Q60819.

CleanEx

MM_IL15RA.

Genevestigator

Q60819.

GermOnline

ENSMUSG00000023206. Mus musculus.

Family and domain databases

InterPro

IPR016060. Complement_control_module.
IPR000436. Sushi_SCR_CCP.
[Graphical view]

Gene3D

G3DSA:2.10.70.10. Complement_control_module. 1 hit.

KO

K05074.

Pfam

PF00084. Sushi. 1 hit.
[Graphical view]

SMART

SM00032. CCP. 1 hit.
[Graphical view]

SUPFAM

SSF57535. Complement_control_module. 1 hit.

PROSITE

PS50923. SUSHI. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

289007.

PMAP-CutDB

Q60819.

SOURCE

Search...

Entry information

Entry name

I15RA_MOUSE

Accession

Primary (citable) accession number: Q60819
Secondary accession number(s): A2AP35 , A2AP36, A2AP37, Q80Z90, Q80Z91, Q80Z92, Q8R5E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program