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Protein

Nascent polypeptide-associated complex subunit alpha

Gene

Naca

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) (By similarity). Isoform 1 and isoform 2 appear to bind DNA and play roles in transcription. Isoform 1 may function as a specific coactivator for JUN, acting to stabilize the interaction of JUN homodimers with promoter elements.By similarity5 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • TBP-class protein binding Source: MGI
  • transcription coactivator activity Source: MGI

GO - Biological processi

  • cardiac ventricle development Source: BHF-UCL
  • heart trabecula morphogenesis Source: BHF-UCL
  • myoblast migration Source: MGI
  • negative regulation of striated muscle cell apoptotic process Source: BHF-UCL
  • negative regulation of transcription from RNA polymerase II promoter involved in heart development Source: BHF-UCL
  • positive regulation of cell proliferation involved in heart morphogenesis Source: BHF-UCL
  • positive regulation of skeletal muscle tissue growth Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter involved in heart development Source: BHF-UCL
  • protein transport Source: UniProtKB-KW
  • regulation of skeletal muscle fiber development Source: BHF-UCL
  • skeletal muscle tissue regeneration Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChaperone, DNA-binding
Biological processProtein transport, Transcription, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nascent polypeptide-associated complex subunit alpha
Alternative name(s):
Alpha-NAC
Alpha-NAC/1.9.2
Gene namesi
Name:Naca
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:106095. Naca.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001355771 – 215Nascent polypeptide-associated complex subunit alphaAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43Phosphoserine; by ILK11 Publication1
Modified residuei132PhosphoserineBy similarity1
Modified residuei142N6-acetyllysine; alternateCombined sources1
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei159Phosphothreonine; by GSK3-betaCombined sources1 Publication1
Modified residuei161PhosphothreonineBy similarity1
Modified residuei166PhosphoserineCombined sources1
Modified residuei186PhosphoserineBy similarity1
Modified residuei191PhosphoserineBy similarity1
Modified residuei203PhosphoserineBy similarity1
Modified residuei214PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation.2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ60817.
PaxDbiQ60817.
PeptideAtlasiQ60817.
PRIDEiQ60817.

PTM databases

iPTMnetiQ60817.

Expressioni

Tissue specificityi

Isoform 1 appears to be ubiquitously expressed.3 Publications

Developmental stagei

Expressed concomitant with the onset of mineralization in ossification centers of developing bone.1 Publication

Gene expression databases

BgeeiENSMUSG00000061315.
ExpressionAtlasiQ60817. baseline and differential.
GenevisibleiQ60817. MM.

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit and contacts the ribosomal protein L23, which is positioned near the exiting site. Both subunits can contact nascent polypeptide chains. NACA may also form homodimers, and only this form binds DNA (By similarity). Interacts with TBP and JUN.By similarity3 Publications

GO - Molecular functioni

  • TBP-class protein binding Source: MGI

Protein-protein interaction databases

BioGridi201682. 9 interactors.
IntActiQ60817. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ60817.
SMRiQ60817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini70 – 135NAC-A/BPROSITE-ProRule annotationAdd BLAST66
Domaini176 – 213UBAAdd BLAST38

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 80Required for DNA-bindingAdd BLAST12
Regioni93 – 108RNA/DNA-bindingBy similarityAdd BLAST16

Domaini

The positively charged inner surface of the NAC-A/B domain is crucial for NACA localization in the nucleus and DNA-binding. This region is blocked from binding nucleic acids in the heterodimeric complex by a helix region in the beta-subunit, it also displays much higher affinity for RNA than DNA (By similarity).By similarity

Sequence similaritiesi

Belongs to the NAC-alpha family.Curated

Phylogenomic databases

eggNOGiKOG2239. Eukaryota.
COG1308. LUCA.
GeneTreeiENSGT00440000033468.
HOGENOMiHOG000239674.
HOVERGENiHBG082004.

Family and domain databases

InterProiView protein in InterPro
IPR016641. EGD2/NACA.
IPR002715. Nas_poly-pep-assoc_cplx_dom.
PANTHERiPTHR21713. PTHR21713. 1 hit.
PfamiView protein in Pfam
PF01849. NAC. 1 hit.
PIRSFiPIRSF015901. NAC_alpha. 1 hit.
SMARTiView protein in SMART
SM01407. NAC. 1 hit.
PROSITEiView protein in PROSITE
PS51151. NAC_AB. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60817-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQTATQQ
60 70 80 90 100
AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK
110 120 130 140 150
NILFVITKPD VYKSPASDTY IVFGEAKIED LSQQAQLAAA EKFKVQGEAV
160 170 180 190 200
SNIQENTQTP TVQEESEEEE VDETGVEVKD IELVMSQANV SRAKAVRALK
210
NNSNDIVNAI MELTM
Length:215
Mass (Da):23,384
Last modified:November 1, 1996 - v1
Checksum:i0E7D5D90304E306D
GO
Isoform 2 (identifier: P70670-1) [UniParc]FASTAAdd to basket
Also known as: Gp220, skNAC
The sequence of this isoform can be found in the external entry P70670.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:2,187
Mass (Da):220,499
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52Q → K in BAE40130 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22151 mRNA. Translation: AAB80961.1.
U48363 Genomic DNA. Translation: AAB18733.1.
AK146197 mRNA. Translation: BAE26971.1.
AK151928 mRNA. Translation: BAE30804.1.
AK161678 mRNA. Translation: BAE36526.1.
AK168023 mRNA. Translation: BAE40008.1.
AK168169 mRNA. Translation: BAE40130.1.
AK168607 mRNA. Translation: BAE40474.1.
AK169432 mRNA. Translation: BAE41173.1.
AK169435 mRNA. Translation: BAE41176.1.
BC029830 mRNA. Translation: AAH29830.1.
BC083340 mRNA. Translation: AAH83340.1.
BC099375 mRNA. Translation: AAH99375.1.
CCDSiCCDS24258.1. [Q60817-1]
PIRiT30827.
RefSeqiNP_001269905.1. NM_001282976.1. [Q60817-1]
NP_038636.2. NM_013608.3. [Q60817-1]
UniGeneiMm.3746.

Genome annotation databases

EnsembliENSMUST00000073868; ENSMUSP00000073532; ENSMUSG00000061315. [Q60817-1]
GeneIDi17938.
UCSCiuc007hlb.2. mouse. [Q60817-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNACA_MOUSE
AccessioniPrimary (citable) accession number: Q60817
Secondary accession number(s): Q3THR6, Q4FZL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families