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Protein

CCR4-NOT transcription complex subunit 7

Gene

Cnot7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for miRNA-mediated mRNA deadenylation. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.2 Publications

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Mn2+By similarity, Mg2+By similarity, Co2+By similarityNote: Binds 2 divalent metal cations per subunit with RNAase activity being higher in presence of Mn2+ than of Mg2+ or Co2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Divalent metal cation 1; catalyticBy similarity
Metal bindingi40 – 401Divalent metal cation 2; catalyticBy similarity
Metal bindingi42 – 421Divalent metal cation 2; catalyticBy similarity
Metal bindingi161 – 1611Divalent metal cation 1; catalyticBy similarity
Metal bindingi230 – 2301Divalent metal cation 2; catalyticBy similarity
Metal bindingi278 – 2781Divalent metal cation 1; catalyticBy similarity

GO - Molecular functioni

  • 3'-5'-exoribonuclease activity Source: UniProtKB
  • exoribonuclease activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • poly(A)-specific ribonuclease activity Source: MGI
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • cytoplasmic mRNA processing body assembly Source: MGI
  • deadenylation-dependent decapping of nuclear-transcribed mRNA Source: MGI
  • exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay Source: UniProtKB
  • gene silencing by RNA Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • negative regulation of gene expression Source: MGI
  • nuclear-transcribed mRNA poly(A) tail shortening Source: MGI
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of mRNA catabolic process Source: MGI
  • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
  • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of translation Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease, Repressor

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 7 (EC:3.1.13.4)
Alternative name(s):
CCR4-associated factor 1
Short name:
CAF-1
Gene namesi
Name:Cnot7
Synonyms:Caf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1298230. Cnot7.

Subcellular locationi

GO - Cellular componenti

  • CCR4-NOT complex Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic mRNA processing body Source: UniProtKB
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285CCR4-NOT transcription complex subunit 7PRO_0000212845Add
BLAST

Proteomic databases

EPDiQ60809.
MaxQBiQ60809.
PaxDbiQ60809.
PRIDEiQ60809.

PTM databases

iPTMnetiQ60809.
PhosphoSiteiQ60809.

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000031601.
CleanExiMM_CNOT7.
ExpressionAtlasiQ60809. baseline and differential.
GenevisibleiQ60809. MM.

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8. In the complex, interacts directly with CNOT1. Interacts with AGO2, TOB1, BTG1, BTG2 and NANOS2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BTG1P623245EBI-2104739,EBI-742279From a different organism.
BTG2P785435EBI-2104739,EBI-1047576From a different organism.
CNOT11Q9UKZ13EBI-2104739,EBI-2562014From a different organism.
Cnot6lQ8VEG62EBI-2104739,EBI-2104661

Protein-protein interaction databases

BioGridi202298. 4 interactions.
DIPiDIP-46843N.
IntActiQ60809. 24 interactions.
MINTiMINT-4597058.
STRINGi10090.ENSMUSP00000034012.

Structurei

3D structure databases

ProteinModelPortaliQ60809.
SMRiQ60809. Positions 11-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CAF1 family.Curated

Phylogenomic databases

eggNOGiKOG0304. Eukaryota.
COG5228. LUCA.
GeneTreeiENSGT00390000000080.
HOGENOMiHOG000173077.
HOVERGENiHBG051044.
InParanoidiQ60809.
KOiK12581.
OMAiGIRDVWG.
OrthoDBiEOG091G0M48.
PhylomeDBiQ60809.
TreeFamiTF314185.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR006941. RNase_CAF1.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF04857. CAF1. 2 hits.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60809-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAATVDHSQ RICEVWACNL DEEMKKIRQV IRKYNYVAMD TEFPGVVARP
60 70 80 90 100
IGEFRSNADY QYQLLRCNVD LLKIIQLGLT FMNEQGEYPP GTSTWQFNFK
110 120 130 140 150
FNLTEDMYAQ DSIELLTTSG IQFKKHEEEG IETQYFAELL MTSGVVLCEG
160 170 180 190 200
VKWLSFHSGY DFGYLIKILT NSNLPEEELD FFEILRLFFP VIYDVKYLMK
210 220 230 240 250
SCKNLKGGLQ EVAEQLELER IGPQHQAGSD SLLTGMAFFK MREMFFEDHI
260 270 280
DDAKYCGHLY GLGSGSSYVQ NGTGNAYEEE ASKQS
Length:285
Mass (Da):32,718
Last modified:November 1, 1996 - v1
Checksum:i2AF23EC38E06EFDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21855 mRNA. Translation: AAA87455.1.
BC006021 mRNA. Translation: AAH06021.1.
CCDSiCCDS22253.1.
RefSeqiNP_001258471.1. NM_001271542.1.
NP_035265.1. NM_011135.5.
XP_011240485.1. XM_011242183.1.
UniGeneiMm.272551.

Genome annotation databases

EnsembliENSMUST00000034012; ENSMUSP00000034012; ENSMUSG00000031601.
ENSMUST00000132032; ENSMUSP00000122933; ENSMUSG00000031601.
ENSMUST00000149992; ENSMUSP00000117304; ENSMUSG00000031601.
GeneIDi18983.
KEGGimmu:18983.
UCSCiuc009lmq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21855 mRNA. Translation: AAA87455.1.
BC006021 mRNA. Translation: AAH06021.1.
CCDSiCCDS22253.1.
RefSeqiNP_001258471.1. NM_001271542.1.
NP_035265.1. NM_011135.5.
XP_011240485.1. XM_011242183.1.
UniGeneiMm.272551.

3D structure databases

ProteinModelPortaliQ60809.
SMRiQ60809. Positions 11-262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202298. 4 interactions.
DIPiDIP-46843N.
IntActiQ60809. 24 interactions.
MINTiMINT-4597058.
STRINGi10090.ENSMUSP00000034012.

PTM databases

iPTMnetiQ60809.
PhosphoSiteiQ60809.

Proteomic databases

EPDiQ60809.
MaxQBiQ60809.
PaxDbiQ60809.
PRIDEiQ60809.

Protocols and materials databases

DNASUi18983.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034012; ENSMUSP00000034012; ENSMUSG00000031601.
ENSMUST00000132032; ENSMUSP00000122933; ENSMUSG00000031601.
ENSMUST00000149992; ENSMUSP00000117304; ENSMUSG00000031601.
GeneIDi18983.
KEGGimmu:18983.
UCSCiuc009lmq.2. mouse.

Organism-specific databases

CTDi29883.
MGIiMGI:1298230. Cnot7.

Phylogenomic databases

eggNOGiKOG0304. Eukaryota.
COG5228. LUCA.
GeneTreeiENSGT00390000000080.
HOGENOMiHOG000173077.
HOVERGENiHBG051044.
InParanoidiQ60809.
KOiK12581.
OMAiGIRDVWG.
OrthoDBiEOG091G0M48.
PhylomeDBiQ60809.
TreeFamiTF314185.

Enzyme and pathway databases

ReactomeiR-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Miscellaneous databases

ChiTaRSiCnot7. mouse.
PROiQ60809.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031601.
CleanExiMM_CNOT7.
ExpressionAtlasiQ60809. baseline and differential.
GenevisibleiQ60809. MM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR006941. RNase_CAF1.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF04857. CAF1. 2 hits.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCNOT7_MOUSE
AccessioniPrimary (citable) accession number: Q60809
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.