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Protein

Serine/threonine-protein kinase PLK3

Gene

Plk3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921ATPPROSITE-ProRule annotation
Active sitei186 – 1861Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi69 – 779ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • p53 binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle, DNA damage

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.21. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PLK3 (EC:2.7.11.21)
Alternative name(s):
Cytokine-inducible serine/threonine-protein kinase
FGF-inducible kinase
Polo-like kinase 3
Short name:
PLK-3
Gene namesi
Name:Plk3
Synonyms:Cnk, Fnk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109604. Plk3.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • chromatin Source: MGI
  • cytoplasm Source: UniProtKB
  • Golgi stack Source: UniProtKB
  • intracellular Source: UniProtKB
  • nucleolus Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Nucleus

Pathology & Biotechi

Disruption phenotypei

Aging mice display increased weight. Cells display defects in the G1/S cell cycle checkpoint and CDC25A protein is more stable. According to a report, they also develop tumors with the highest incidence in the lung but also in the kidney, the liver and the uterus (PubMed:18519666). However, increased tumorigenesis was not observed by another report (PubMed:21376736;). The differences observed might be due to the different mouse strain background used in the 2 experiments.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 631631Serine/threonine-protein kinase PLK3PRO_0000086565Add
BLAST

Post-translational modificationi

Phosphorylated in an ATM-dependent manner following DNA damage (By similarity). Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60806.
PRIDEiQ60806.

PTM databases

iPTMnetiQ60806.
PhosphoSiteiQ60806.

Expressioni

Tissue specificityi

Expressed in skin.

Inductioni

Negatively regulated by TTP family members: TTP binds to the 3'-untranslated region (3'-UTR) of PLK3 mRNAs, contributing to the rapid degradation of transcripts.1 Publication

Gene expression databases

CleanExiMM_PLK3.

Interactioni

Subunit structurei

Interacts (via the POLO-box domain) with CIB1; leading to inhibit PLK3 kinase activity. Interacts with GOLGB1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076130.

Structurei

3D structure databases

ProteinModelPortaliQ60806.
SMRiQ60806. Positions 53-359, 414-625.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 315253Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini455 – 51864POLO box 1PROSITE-ProRule annotationAdd
BLAST
Domaini552 – 62271POLO box 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The POLO box domains act as phosphopeptide-binding module that recognize and bind serine-[phosphothreonine/phosphoserine]-(proline/X) motifs. PLK3 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them. The POLO box domains mediates localization to the centrosome (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.PROSITE-ProRule annotation
Contains 2 POLO box domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0575. Eukaryota.
ENOG410XQBP. LUCA.
HOVERGENiHBG001843.
InParanoidiQ60806.

Family and domain databases

CDDicd13118. POLO_box_1. 1 hit.
cd13117. POLO_box_2. 1 hit.
Gene3Di3.30.1120.30. 2 hits.
InterProiIPR011009. Kinase-like_dom.
IPR033701. POLO_box_1.
IPR033695. POLO_box_2.
IPR000959. POLO_box_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR020658. Ser/Thr_kinase_Plk3.
[Graphical view]
PANTHERiPTHR24345:SF42. PTHR24345:SF42. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
PF00659. POLO_box. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50078. POLO_BOX. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60806-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPAAGFLSP RPFPRAAVPS APPAGPGPPA NASPRSEPEV LAGPRAPDPP
60 70 80 90 100
GRLITDPLSG RTYTKGRLLG KGGFARCYEA TDTESGIAYA VKVIPQSRVA
110 120 130 140 150
KPHQREKILN EIELHRDLQH RHIVRFSHHF EDADNIYIFL ELCSRKSLAH
160 170 180 190 200
IWKARHTLLE PEVRYYLRQI LSGLKYLHQR GILHRDLKLG NFFITDNMEL
210 220 230 240 250
KVGDFGLAAR LEPPEQRKKT ICGTPNYVAP EVLLRQGHGP EADVWSLGCV
260 270 280 290 300
MYTLLCGSPP FETADLKETY RCIKQVHYTL PASLSLPARQ LLAAILRASP
310 320 330 340 350
RDRPSIEQIL RHDFFTKGYT PDRLPVSSCV TVPDLTPPNP ARSLFAKVTK
360 370 380 390 400
SLFGRKKNKN KNHSEDQDNV SCLAPVVSGQ APASLIETAA EDSSPRGTLA
410 420 430 440 450
SSGDGFEEGL TVATVVESAL CALRNCVAFM PPAEQNPAPL AQPEPLVWVS
460 470 480 490 500
KWVDYSNKFG FGYQLSSRRV AVLFNDGTHM ALSANRKTVH YNPTSTKHFS
510 520 530 540 550
FSMGSVPRAL QPQLGILRYF ASYMEQHLMK GGDLPSVEEA EVPAPPLLLQ
560 570 580 590 600
WVKTDQALLM LFSDGTVQVN FYGDHTKLIL SGWEPLLVTF VARNRSACTY
610 620 630
LASHLRQLGC SPDLRQRLRY ALRLLRDQSP A
Length:631
Mass (Da):70,012
Last modified:April 27, 2001 - v2
Checksum:i20857341870DB1D2
GO
Isoform 2 (identifier: Q60806-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     373-373: L → LVSGLMRTSIGHPDVRPE

Show »
Length:648
Mass (Da):71,845
Checksum:i8EA657419CA426F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti386 – 3861I → V in AAF08369 (PubMed:11039900).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei373 – 3731L → LVSGLMRTSIGHPDVRPE in isoform 2. 1 PublicationVSP_004927

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21392 mRNA. Translation: AAC52191.1.
U22434 Genomic DNA. Translation: AAC52192.1.
AF136586 mRNA. Translation: AAF08369.1.
CCDSiCCDS18529.1. [Q60806-2]
PIRiA57286.
UniGeneiMm.259022.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21392 mRNA. Translation: AAC52191.1.
U22434 Genomic DNA. Translation: AAC52192.1.
AF136586 mRNA. Translation: AAF08369.1.
CCDSiCCDS18529.1. [Q60806-2]
PIRiA57286.
UniGeneiMm.259022.

3D structure databases

ProteinModelPortaliQ60806.
SMRiQ60806. Positions 53-359, 414-625.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076130.

PTM databases

iPTMnetiQ60806.
PhosphoSiteiQ60806.

Proteomic databases

PaxDbiQ60806.
PRIDEiQ60806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:109604. Plk3.

Phylogenomic databases

eggNOGiKOG0575. Eukaryota.
ENOG410XQBP. LUCA.
HOVERGENiHBG001843.
InParanoidiQ60806.

Enzyme and pathway databases

BRENDAi2.7.11.21. 3474.

Miscellaneous databases

PROiQ60806.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PLK3.

Family and domain databases

CDDicd13118. POLO_box_1. 1 hit.
cd13117. POLO_box_2. 1 hit.
Gene3Di3.30.1120.30. 2 hits.
InterProiIPR011009. Kinase-like_dom.
IPR033701. POLO_box_1.
IPR033695. POLO_box_2.
IPR000959. POLO_box_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR020658. Ser/Thr_kinase_Plk3.
[Graphical view]
PANTHERiPTHR24345:SF42. PTHR24345:SF42. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
PF00659. POLO_box. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50078. POLO_BOX. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLK3_MOUSE
AccessioniPrimary (citable) accession number: Q60806
Secondary accession number(s): Q60822, Q9R009
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.