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Protein

Ras GTPase-activating protein 3

Gene

Rasa3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway. May bind inositol tetrakisphosphate (IP4).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri679 – 71537Btk-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 3
Alternative name(s):
GAP1(IP4BP)
GapIII
Ins P4-binding protein
Gene namesi
Name:Rasa3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1197013. Rasa3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 834834Ras GTPase-activating protein 3PRO_0000056643Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661PhosphotyrosineCombined sources
Modified residuei77 – 771PhosphoserineCombined sources
Modified residuei110 – 1101PhosphothreonineBy similarity
Modified residuei809 – 8091PhosphoserineCombined sources
Modified residuei833 – 8331PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ60790.
MaxQBiQ60790.
PaxDbiQ60790.
PRIDEiQ60790.

PTM databases

iPTMnetiQ60790.
PhosphoSiteiQ60790.

Expressioni

Tissue specificityi

High levels in brain, lower in spleen and lung.

Gene expression databases

BgeeiQ60790.
CleanExiMM_RASA3.
ExpressionAtlasiQ60790. baseline and differential.
GenevisibleiQ60790. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112998.

Structurei

3D structure databases

ProteinModelPortaliQ60790.
SMRiQ60790. Positions 11-269, 298-568, 580-709.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9696C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini132 – 247116C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini330 – 524195Ras-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini576 – 677102PHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Btk-type zinc finger.PROSITE-ProRule annotation
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri679 – 71537Btk-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2059. Eukaryota.
ENOG410Y128. LUCA.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000007183.
HOVERGENiHBG055643.
InParanoidiQ60790.
KOiK12380.
OMAiSHKSCEI.
OrthoDBiEOG7J17Z6.
TreeFamiTF105302.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 2 hits.
[Graphical view]
SMARTiSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVEEEGLRV FQSVRIKIGE AKNLPSYPGP NKMRDCYCTV NLDQEEVFRT
60 70 80 90 100
KIVEKSLCPF YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE
110 120 130 140 150
DLQRYHNRDT WFQLQHVDAD SEVQGKVHLE LRLSEVITDT GVVCHKLAAR
160 170 180 190 200
IFECQGLPIV NGQCDPYATV TLAGPFRSEA KKTKVKKKTN NPQFDEVFYF
210 220 230 240 250
EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD EFLGELRLPL
260 270 280 290 300
KILRHSSSYE AWYFLQPRDN GNKSLKPDDL GSLRLNVVYT EDHVFSSEYY
310 320 330 340 350
SPLRDLLLKS ADVEPVSASA AHILGEVCRD KQEAAIPLVR LLLHYGRVVP
360 370 380 390 400
FISAIASAEV KRTQDPNTIF RGNSLTSKCI DETMKLAGMH YLHVTLKPTI
410 420 430 440 450
EEICQSHKSC EIDPVKLKDG ENLENNMESL RQYVDRIFTV ITKSGVSCPT
460 470 480 490 500
VMCDIFFSLR EAAAKRFQDD LDVRYTAVSS FIFLRFFAPA ILSPNLFQLT
510 520 530 540 550
PHHTDPQTSR TLTLISKTIQ TLGSLSKSKS ASFKESYMAT FYEFFNEQKY
560 570 580 590 600
ADAVKNFLDL ISSSGRRDPK SIEQPILLKE GFMIKRAQGR KRFGMKNFKK
610 620 630 640 650
RWFRLTNHEF TYQKSKGDQP LCNIPIENIL AVERLEEESF RMKNMFQVIQ
660 670 680 690 700
PERALYIQAN NCVEAKDWID ILTKVSQCNQ KRLTVFHPSA YLNGHWLCCR
710 720 730 740 750
ASSDTAAGCT PCTGGLPANI QLDIDGDRET ERIYSLFNLY MGKLEKMQEA
760 770 780 790 800
CGSKSVYDGP EQEEYSTFVI DDPQETYKTL KQVIAGVGTL EQEHAQYRRD
810 820 830
KFKKTRYGSQ EHPIGDKSFQ NYIRQQSEIS THSI
Length:834
Mass (Da):95,987
Last modified:July 27, 2011 - v2
Checksum:i99FE01CAE4F1AE2F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391D → H in AAA93008 (PubMed:7500386).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20238 mRNA. Translation: AAA93008.1.
AB052362 Genomic DNA. Translation: BAB55802.1.
AK141511 mRNA. Translation: BAE24707.1.
CH466566 Genomic DNA. Translation: EDL22155.1.
BC057300 mRNA. Translation: AAH57300.2.
BC068297 mRNA. Translation: AAH68297.1.
CCDSiCCDS40234.1.
RefSeqiNP_033051.2. NM_009025.2.
UniGeneiMm.18517.

Genome annotation databases

EnsembliENSMUST00000117551; ENSMUSP00000112998; ENSMUSG00000031453.
GeneIDi19414.
KEGGimmu:19414.
UCSCiuc009kye.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20238 mRNA. Translation: AAA93008.1.
AB052362 Genomic DNA. Translation: BAB55802.1.
AK141511 mRNA. Translation: BAE24707.1.
CH466566 Genomic DNA. Translation: EDL22155.1.
BC057300 mRNA. Translation: AAH57300.2.
BC068297 mRNA. Translation: AAH68297.1.
CCDSiCCDS40234.1.
RefSeqiNP_033051.2. NM_009025.2.
UniGeneiMm.18517.

3D structure databases

ProteinModelPortaliQ60790.
SMRiQ60790. Positions 11-269, 298-568, 580-709.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112998.

PTM databases

iPTMnetiQ60790.
PhosphoSiteiQ60790.

Proteomic databases

EPDiQ60790.
MaxQBiQ60790.
PaxDbiQ60790.
PRIDEiQ60790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117551; ENSMUSP00000112998; ENSMUSG00000031453.
GeneIDi19414.
KEGGimmu:19414.
UCSCiuc009kye.1. mouse.

Organism-specific databases

CTDi22821.
MGIiMGI:1197013. Rasa3.

Phylogenomic databases

eggNOGiKOG2059. Eukaryota.
ENOG410Y128. LUCA.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000007183.
HOVERGENiHBG055643.
InParanoidiQ60790.
KOiK12380.
OMAiSHKSCEI.
OrthoDBiEOG7J17Z6.
TreeFamiTF105302.

Enzyme and pathway databases

ReactomeiR-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

PROiQ60790.
SOURCEiSearch...

Gene expression databases

BgeeiQ60790.
CleanExiMM_RASA3.
ExpressionAtlasiQ60790. baseline and differential.
GenevisibleiQ60790. MM.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 2 hits.
[Graphical view]
SMARTiSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GapIII, a new brain-enriched member of the GTPase-activating protein family."
    Baba H., Fuss B., Urano J., Poullet P., Watson J.B., Tamanoi F., Macklin W.B.
    J. Neurosci. Res. 41:846-858(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Murine R ras GAP cDNA construct."
    Iwashita S., Sezaki M.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Spinal cord.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and CD-1.
    Tissue: Brain and Neural stem cell.
  6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-66, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-809 AND SER-833, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Lung and Spleen.

Entry informationi

Entry nameiRASA3_MOUSE
AccessioniPrimary (citable) accession number: Q60790
Secondary accession number(s): Q6PG24, Q925V1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.