Q60790 (RASA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras GTPase-activating protein 3

Alternative name(s):
GAP1(IP4BP)
GapIII
Ins P4-binding protein

Gene names

Name:

Rasa3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitory regulator of the Ras-cyclic AMP pathway. May bind inositol tetrakisphosphate (IP4).
Tissue specificity	High levels in brain, lower in spleen and lung.
Sequence similarities	Contains 1 Btk-type zinc finger. Contains 2 C2 domains. Contains 1 PH domain. Contains 1 Ras-GAP domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro negative regulation of Ras protein signal transduction Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome intrinsic to internal side of plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	Ras GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome calcium-release channel activity Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	Ras GTPase-activating protein 3		PRO_0000056643
Regions
	Domain	1 – 96	96	C2 1
	Domain	132 – 247	116	C2 2
	Domain	330 – 524	195	Ras-GAP
	Domain	576 – 677	102	PH
	Zinc finger	679 – 715	37	Btk-type
Amino acid modifications
	Modified residue	66	1	Phosphotyrosine Ref.6
	Modified residue	110	1	Phosphothreonine By similarity
	Modified residue	809	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	139	1	D → H in AAA93008. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q60790 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 99FE01CAE4F1AE2F	FASTA	834	95,987
	10 20 30 40 50 60 MAVEEEGLRV FQSVRIKIGE AKNLPSYPGP NKMRDCYCTV NLDQEEVFRT KIVEKSLCPF 70 80 90 100 110 120 YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE DLQRYHNRDT WFQLQHVDAD 130 140 150 160 170 180 SEVQGKVHLE LRLSEVITDT GVVCHKLAAR IFECQGLPIV NGQCDPYATV TLAGPFRSEA 190 200 210 220 230 240 KKTKVKKKTN NPQFDEVFYF EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD 250 260 270 280 290 300 EFLGELRLPL KILRHSSSYE AWYFLQPRDN GNKSLKPDDL GSLRLNVVYT EDHVFSSEYY 310 320 330 340 350 360 SPLRDLLLKS ADVEPVSASA AHILGEVCRD KQEAAIPLVR LLLHYGRVVP FISAIASAEV 370 380 390 400 410 420 KRTQDPNTIF RGNSLTSKCI DETMKLAGMH YLHVTLKPTI EEICQSHKSC EIDPVKLKDG 430 440 450 460 470 480 ENLENNMESL RQYVDRIFTV ITKSGVSCPT VMCDIFFSLR EAAAKRFQDD LDVRYTAVSS 490 500 510 520 530 540 FIFLRFFAPA ILSPNLFQLT PHHTDPQTSR TLTLISKTIQ TLGSLSKSKS ASFKESYMAT 550 560 570 580 590 600 FYEFFNEQKY ADAVKNFLDL ISSSGRRDPK SIEQPILLKE GFMIKRAQGR KRFGMKNFKK 610 620 630 640 650 660 RWFRLTNHEF TYQKSKGDQP LCNIPIENIL AVERLEEESF RMKNMFQVIQ PERALYIQAN 670 680 690 700 710 720 NCVEAKDWID ILTKVSQCNQ KRLTVFHPSA YLNGHWLCCR ASSDTAAGCT PCTGGLPANI 730 740 750 760 770 780 QLDIDGDRET ERIYSLFNLY MGKLEKMQEA CGSKSVYDGP EQEEYSTFVI DDPQETYKTL 790 800 810 820 830 KQVIAGVGTL EQEHAQYRRD KFKKTRYGSQ EHPIGDKSFQ NYIRQQSEIS THSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GapIII, a new brain-enriched member of the GTPase-activating protein family." Baba H., Fuss B., Urano J., Poullet P., Watson J.B., Tamanoi F., Macklin W.B. J. Neurosci. Res. 41:846-858(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Murine R ras GAP cDNA construct." Iwashita S., Sezaki M. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Spinal cord.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and CD-1. Tissue: Brain and Neural stem cell.
[6]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-66, MASS SPECTROMETRY. Tissue: Mast cell.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-809, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20238 mRNA. Translation: AAA93008.1. AB052362 Genomic DNA. Translation: BAB55802.1. AK141511 mRNA. Translation: BAE24707.1. CH466566 Genomic DNA. Translation: EDL22155.1. BC057300 mRNA. Translation: AAH57300.2. BC068297 mRNA. Translation: AAH68297.1.
IPI	IPI00468396.
RefSeq	NP_033051.2. NM_009025.2.
UniGene	Mm.18517.
3D structure databases
ProteinModelPortal	Q60790.
SMR	Q60790. Positions 11-202, 298-568, 580-709.
ModBase	Search...
PTM databases
PhosphoSite	Q60790.
Proteomic databases
PaxDb	Q60790.
PRIDE	Q60790.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000117551; ENSMUSP00000112998; ENSMUSG00000031453.
GeneID	19414.
KEGG	mmu:19414.
Organism-specific databases
CTD	22821.
MGI	MGI:1197013. Rasa3.
Phylogenomic databases
eggNOG	NOG323905.
GeneTree	ENSGT00600000084336.
HOGENOM	HOG000007183.
HOVERGEN	HBG055643.
KO	K12380.
OMA	APIGDDN.
Gene expression databases
ArrayExpress	Q60790.
Bgee	Q60790.
CleanEx	MM_RASA3.
Genevestigator	Q60790.
GermOnline	ENSMUSG00000031453. Mus musculus.
Family and domain databases
Gene3D	1.10.506.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR001936. RasGAP. IPR023152. RasGAP_CS. IPR008936. Rho_GTPase_activation_prot. IPR001562. Znf_Btk_motif. [Graphical view]
Pfam	PF00779. BTK. 1 hit. PF00168. C2. 2 hits. PF00169. PH. 1 hit. PF00616. RasGAP. 1 hit. [Graphical view]
SMART	SM00107. BTK. 1 hit. SM00239. C2. 2 hits. SM00233. PH. 1 hit. SM00323. RasGAP. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 2 hits. SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50004. C2. 2 hits. PS50003. PH_DOMAIN. 1 hit. PS00509. RAS_GTPASE_ACTIV_1. 1 hit. PS50018. RAS_GTPASE_ACTIV_2. 1 hit. PS51113. ZF_BTK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	296541.
SOURCE	Search...

Entry information

Entry name

RASA3_MOUSE

Accession

Primary (citable) accession number: Q60790
Secondary accession number(s): Q6PG24, Q925V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program