Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q60778

- IKBB_MOUSE

UniProt

Q60778 - IKBB_MOUSE

Protein

NF-kappa-B inhibitor beta

Gene

Nfkbib

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    Enzyme and pathway databases

    ReactomeiREACT_199121. Activation of NF-kappaB in B cells.
    REACT_202898. TRAF6 mediated NF-kB activation.
    REACT_219800. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
    REACT_222971. RIP-mediated NFkB activation via ZBP1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NF-kappa-B inhibitor beta
    Short name:
    NF-kappa-BIB
    Alternative name(s):
    I-kappa-B-beta
    Short name:
    IkB-B
    Short name:
    IkB-beta
    Short name:
    IkappaBbeta
    Gene namesi
    Name:Nfkbib
    Synonyms:Ikbb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:104752. Nfkbib.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 359359NF-kappa-B inhibitor betaPRO_0000067005Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191Phosphoserine; by RPS6KA1By similarity
    Modified residuei23 – 231Phosphoserine; by RPS6KA1By similarity
    Modified residuei313 – 3131PhosphoserineBy similarity
    Modified residuei318 – 3181PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ60778.
    PRIDEiQ60778.

    PTM databases

    PhosphoSiteiQ60778.

    Expressioni

    Tissue specificityi

    Highly expressed in testis followed by spleen.

    Gene expression databases

    ArrayExpressiQ60778.
    BgeeiQ60778.
    GenevestigatoriQ60778.

    Interactioni

    Subunit structurei

    Interacts with COMMD1 and inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2 By similarity. Interacts with the ligand-binding domain of THRB. Interacts with RELA and REL.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Arih2Q9Z1K62EBI-644469,EBI-6861719
    RelaQ042078EBI-644469,EBI-644400

    Protein-protein interaction databases

    BioGridi201754. 3 interactions.
    DIPiDIP-37418N.
    IntActiQ60778. 3 interactions.
    MINTiMINT-1523907.

    Structurei

    Secondary structure

    1
    359
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi61 – 677
    Helixi71 – 8111
    Helixi85 – 884
    Helixi97 – 1048
    Helixi107 – 1159
    Helixi130 – 1345
    Turni135 – 1384
    Helixi140 – 1467
    Beta strandi147 – 1493
    Helixi196 – 1994
    Helixi210 – 2167
    Helixi220 – 22910
    Turni238 – 2403
    Helixi244 – 2507
    Helixi254 – 2629
    Helixi277 – 2826
    Helixi287 – 2959
    Helixi304 – 3074

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K3ZX-ray2.50D50-331[»]
    1OY3X-ray2.05D50-331[»]
    ProteinModelPortaliQ60778.
    SMRiQ60778. Positions 5-346.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ60778.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati57 – 8630ANK 1Add
    BLAST
    Repeati93 – 12230ANK 2Add
    BLAST
    Repeati126 – 15530ANK 3Add
    BLAST
    Repeati206 – 23530ANK 4Add
    BLAST
    Repeati240 – 26930ANK 5Add
    BLAST
    Repeati273 – 30230ANK 6Add
    BLAST

    Sequence similaritiesi

    Belongs to the NF-kappa-B inhibitor family.Curated
    Contains 6 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG0666.
    GeneTreeiENSGT00550000074527.
    HOGENOMiHOG000137336.
    HOVERGENiHBG019039.
    InParanoidiQ564F1.
    KOiK02581.
    OMAiDEWCDSG.
    OrthoDBiEOG7W154S.
    TreeFamiTF320166.

    Family and domain databases

    Gene3Di1.25.40.20. 2 hits.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view]
    PfamiPF00023. Ank. 4 hits.
    [Graphical view]
    PRINTSiPR01415. ANKYRIN.
    SMARTiSM00248. ANK. 6 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60778-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGVACLGKT ADADEWCDSG LGSLGPDAAA PGGPGLGAEL GPELSWAPLV    50
    FGYVTEDGDT ALHLAVIHQH EPFLDFLLGF SAGTEYLDLQ NDLGQTALHL 100
    AAILGEASTV EKLYAAGAGV LVAERGGHTA LHLACRVRAH TCACVLLQPR 150
    PSHPRDASDT YLTQSQDCTP DTSHAPAAVD SQPNPENEEE PRDEDWRLQL 200
    EAENYDGHTP LHVAVIHKDA EMVRLLRDAG ADLNKPEPTC GRTPLHLAVE 250
    AQAASVLELL LKAGADPTAR MYGGRTPLGS ALLRPNPILA RLLRAHGAPE 300
    PEDEDDKLSP CSSSGSDSDS DNRDEGDEYD DIVVHSGRSQ NRQPPSPASK 350
    PLPDDPNPA 359
    Length:359
    Mass (Da):37,965
    Last modified:July 27, 2011 - v2
    Checksum:i0E2B46C89E2404EF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti84 – 841T → H in AAC52166. (PubMed:7867065)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19799 mRNA. Translation: AAC52166.1.
    AK010218 mRNA. Translation: BAB26774.1.
    AK038631 mRNA. Translation: BAC30071.1.
    AK156731 mRNA. Translation: BAE33825.1.
    CH466593 Genomic DNA. Translation: EDL24117.1.
    CH466593 Genomic DNA. Translation: EDL24118.1.
    CCDSiCCDS21054.1.
    RefSeqiNP_035038.2. NM_010908.4.
    XP_006539676.1. XM_006539613.1.
    UniGeneiMm.220333.

    Genome annotation databases

    EnsembliENSMUST00000032815; ENSMUSP00000032815; ENSMUSG00000030595.
    ENSMUST00000085851; ENSMUSP00000083012; ENSMUSG00000030595.
    GeneIDi18036.
    KEGGimmu:18036.
    UCSCiuc009fzr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19799 mRNA. Translation: AAC52166.1 .
    AK010218 mRNA. Translation: BAB26774.1 .
    AK038631 mRNA. Translation: BAC30071.1 .
    AK156731 mRNA. Translation: BAE33825.1 .
    CH466593 Genomic DNA. Translation: EDL24117.1 .
    CH466593 Genomic DNA. Translation: EDL24118.1 .
    CCDSi CCDS21054.1.
    RefSeqi NP_035038.2. NM_010908.4.
    XP_006539676.1. XM_006539613.1.
    UniGenei Mm.220333.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K3Z X-ray 2.50 D 50-331 [» ]
    1OY3 X-ray 2.05 D 50-331 [» ]
    ProteinModelPortali Q60778.
    SMRi Q60778. Positions 5-346.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201754. 3 interactions.
    DIPi DIP-37418N.
    IntActi Q60778. 3 interactions.
    MINTi MINT-1523907.

    PTM databases

    PhosphoSitei Q60778.

    Proteomic databases

    PaxDbi Q60778.
    PRIDEi Q60778.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032815 ; ENSMUSP00000032815 ; ENSMUSG00000030595 .
    ENSMUST00000085851 ; ENSMUSP00000083012 ; ENSMUSG00000030595 .
    GeneIDi 18036.
    KEGGi mmu:18036.
    UCSCi uc009fzr.1. mouse.

    Organism-specific databases

    CTDi 4793.
    MGIi MGI:104752. Nfkbib.

    Phylogenomic databases

    eggNOGi COG0666.
    GeneTreei ENSGT00550000074527.
    HOGENOMi HOG000137336.
    HOVERGENi HBG019039.
    InParanoidi Q564F1.
    KOi K02581.
    OMAi DEWCDSG.
    OrthoDBi EOG7W154S.
    TreeFami TF320166.

    Enzyme and pathway databases

    Reactomei REACT_199121. Activation of NF-kappaB in B cells.
    REACT_202898. TRAF6 mediated NF-kB activation.
    REACT_219800. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
    REACT_222971. RIP-mediated NFkB activation via ZBP1.

    Miscellaneous databases

    EvolutionaryTracei Q60778.
    NextBioi 293133.
    PROi Q60778.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q60778.
    Bgeei Q60778.
    Genevestigatori Q60778.

    Family and domain databases

    Gene3Di 1.25.40.20. 2 hits.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view ]
    Pfami PF00023. Ank. 4 hits.
    [Graphical view ]
    PRINTSi PR01415. ANKYRIN.
    SMARTi SM00248. ANK. 6 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "IkappaB-beta regulates the persistent response in a biphasic activation of NF-kappaB."
      Thompson J.E., Phillips R.J., Erdjument-Bromage H., Tempst P., Ghosh S.
      Cell 80:573-582(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: B-cell.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Hypothalamus, Spleen and Tongue.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Role of unphosphorylated, newly synthesized IkappaB beta in persistent activation of NF-kappaB."
      Suyang H., Phillips R.J., Douglas I., Ghosh S.
      Mol. Cell. Biol. 16:5444-5449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, INTERACTION WITH RELA AND REL.
    5. "A subclass of Ras proteins that regulate the degradation of IkappaB."
      Fenwick C., Na S.-Y., Voll R.E., Zhong H., Im S.-Y., Lee J.W., Ghosh S.
      Science 287:869-873(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: KAPPA B RAS-BINDING.

    Entry informationi

    Entry nameiIKBB_MOUSE
    AccessioniPrimary (citable) accession number: Q60778
    Secondary accession number(s): Q564F1, Q9D6L5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3