ID   CDN2D_MOUSE             Reviewed;         166 AA.
AC   Q60773; Q60794; Q91YV3;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 2.
DT   27-MAR-2024, entry version 171.
DE   RecName: Full=Cyclin-dependent kinase 4 inhibitor D;
DE   AltName: Full=p19-INK4d;
GN   Name=Cdkn2d;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   STRAIN=C57BL/Kaplan;
RX   PubMed=7739547; DOI=10.1128/mcb.15.5.2672;
RA   Hirai H., Roussel M.F., Kato J.-Y., Ashmun R.A., Sherr C.J.;
RT   "Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-
RT   dependent kinases CDK4 and CDK6.";
RL   Mol. Cell. Biol. 15:2672-2681(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   PubMed=7739548; DOI=10.1128/mcb.15.5.2682;
RA   Chan F.K.M., Zhang J., Cheng L., Shapiro D.N., Winoto A.;
RT   "Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor
RT   with homology to p16ink4.";
RL   Mol. Cell. Biol. 15:2682-2688(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Czech II; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [8]
RP   STRUCTURE BY NMR.
RX   PubMed=9353127; DOI=10.1038/40202;
RA   Luh F.Y., Archer S.J., Domaille P.J., Smith B.O., Owen D., Brotherton D.H.,
RA   Raine A.R., Xu X., Brizuela L., Brenner S.L., Laue E.D.;
RT   "Structure of the cyclin-dependent kinase inhibitor p19Ink4d.";
RL   Nature 389:999-1003(1997).
CC   -!- FUNCTION: Interacts strongly with CDK4 and CDK6 and inhibits them.
CC       {ECO:0000269|PubMed:7739547, ECO:0000269|PubMed:7739548}.
CC   -!- SUBUNIT: Interacts with CDK6. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CDKN2 cyclin-dependent kinase inhibitor
CC       family. {ECO:0000305}.
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DR   EMBL; U19597; AAC52194.1; -; mRNA.
DR   EMBL; U20497; AAA85437.1; -; mRNA.
DR   EMBL; AK132906; BAE21414.1; -; mRNA.
DR   EMBL; AC122525; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH466522; EDL25176.1; -; Genomic_DNA.
DR   EMBL; BC013898; AAH13898.1; -; mRNA.
DR   CCDS; CCDS22901.1; -.
DR   PIR; A57379; A57379.
DR   PIR; B57378; B57378.
DR   RefSeq; NP_034008.2; NM_009878.3.
DR   RefSeq; XP_006510031.1; XM_006509968.1.
DR   PDB; 1AP7; NMR; -; A=1-166.
DR   PDB; 1BLX; X-ray; 1.90 A; B=1-166.
DR   PDBsum; 1AP7; -.
DR   PDBsum; 1BLX; -.
DR   AlphaFoldDB; Q60773; -.
DR   SMR; Q60773; -.
DR   BioGRID; 198657; 2.
DR   CORUM; Q60773; -.
DR   IntAct; Q60773; 1.
DR   STRING; 10090.ENSMUSP00000150701; -.
DR   PhosphoSitePlus; Q60773; -.
DR   EPD; Q60773; -.
DR   PaxDb; 10090-ENSMUSP00000083561; -.
DR   ProteomicsDB; 283868; -.
DR   Pumba; Q60773; -.
DR   Antibodypedia; 4404; 330 antibodies from 34 providers.
DR   DNASU; 12581; -.
DR   Ensembl; ENSMUST00000215619.2; ENSMUSP00000150701.2; ENSMUSG00000096472.3.
DR   GeneID; 12581; -.
DR   KEGG; mmu:12581; -.
DR   UCSC; uc009okw.1; mouse.
DR   AGR; MGI:105387; -.
DR   CTD; 1032; -.
DR   MGI; MGI:105387; Cdkn2d.
DR   VEuPathDB; HostDB:ENSMUSG00000096472; -.
DR   eggNOG; KOG0504; Eukaryota.
DR   GeneTree; ENSGT00940000159801; -.
DR   HOGENOM; CLU_000134_37_0_1; -.
DR   InParanoid; Q60773; -.
DR   OMA; QVMMFGN; -.
DR   OrthoDB; 2321802at2759; -.
DR   PhylomeDB; Q60773; -.
DR   TreeFam; TF333311; -.
DR   BioGRID-ORCS; 12581; 1 hit in 118 CRISPR screens.
DR   EvolutionaryTrace; Q60773; -.
DR   PRO; PR:Q60773; -.
DR   Proteomes; UP000000589; Chromosome 9.
DR   RNAct; Q60773; Protein.
DR   Bgee; ENSMUSG00000096472; Expressed in granulocyte and 261 other cell types or tissues.
DR   ExpressionAtlas; Q60773; baseline and differential.
DR   GO; GO:0097129; C:cyclin D2-CDK4 complex; IDA:MGI.
DR   GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; ISO:MGI.
DR   GO; GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; ISO:MGI.
DR   GO; GO:0019901; F:protein kinase binding; IPI:MGI.
DR   GO; GO:0048102; P:autophagic cell death; ISO:MGI.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0000731; P:DNA synthesis involved in DNA repair; ISO:MGI.
DR   GO; GO:1902807; P:negative regulation of cell cycle G1/S phase transition; IDA:MGI.
DR   GO; GO:0030308; P:negative regulation of cell growth; ISO:MGI.
DR   GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR   GO; GO:1902230; P:negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage; ISO:MGI.
DR   GO; GO:0042326; P:negative regulation of phosphorylation; ISO:MGI.
DR   GO; GO:2000045; P:regulation of G1/S transition of mitotic cell cycle; ISO:MGI.
DR   GO; GO:0032526; P:response to retinoic acid; ISO:MGI.
DR   GO; GO:0009411; P:response to UV; ISO:MGI.
DR   GO; GO:0033280; P:response to vitamin D; ISO:MGI.
DR   GO; GO:0007605; P:sensory perception of sound; IMP:MGI.
DR   Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   PANTHER; PTHR24201; ANK_REP_REGION DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR24201:SF7; CYCLIN-DEPENDENT KINASE 4 INHIBITOR D; 1.
DR   Pfam; PF00023; Ank; 1.
DR   Pfam; PF12796; Ank_2; 1.
DR   SMART; SM00248; ANK; 3.
DR   SUPFAM; SSF48403; Ankyrin repeat; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 1.
DR   Genevisible; Q60773; MM.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; ANK repeat; Cell cycle; Cytoplasm; Nucleus;
KW   Reference proteome; Repeat; Tumor suppressor.
FT   CHAIN           1..166
FT                   /note="Cyclin-dependent kinase 4 inhibitor D"
FT                   /id="PRO_0000144189"
FT   REPEAT          41..69
FT                   /note="ANK 1"
FT   REPEAT          73..102
FT                   /note="ANK 2"
FT   REPEAT          106..135
FT                   /note="ANK 3"
FT   REPEAT          138..165
FT                   /note="ANK 4"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:P55273"
FT   CONFLICT        16
FT                   /note="A -> R (in Ref. 1; AAC52194)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        17
FT                   /note="A -> P (in Ref. 2; AAA85437)"
FT                   /evidence="ECO:0000305"
FT   HELIX           7..17
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   HELIX           21..29
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   HELIX           45..48
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   HELIX           54..62
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   TURN            72..74
FT                   /evidence="ECO:0007829|PDB:1AP7"
FT   HELIX           77..84
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   HELIX           87..95
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   HELIX           110..117
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   HELIX           120..126
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   HELIX           127..129
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   HELIX           142..148
FT                   /evidence="ECO:0007829|PDB:1BLX"
FT   HELIX           152..160
FT                   /evidence="ECO:0007829|PDB:1BLX"
SQ   SEQUENCE   166 AA;  17809 MW;  7B95D6C2294F9259 CRC64;
     MLLEEVCVGD RLSGAAARGD VQEVRRLLHR ELVHPDALNR FGKTALQVMM FGSPAVALEL
     LKQGASPNVQ DASGTSPVHD AARTGFLDTL KVLVEHGADV NALDSTGSLP IHLAIREGHS
     SVVSFLAPES DLHHRDASGL TPLELARQRG AQNLMDILQG HMMIPM
//