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Q60770 (STXB3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Syntaxin-binding protein 3
Alternative name(s):
MUNC-18-3
Mammalian homolog of Unc-18c
Short name=Munc-18c
Protein unc-18 homolog 3
Short name=Unc18-3
Protein unc-18 homolog C
Short name=Unc-18C
Gene names
Name:Stxbp3
Synonyms:Stxbp3a, Unc18c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length592 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. Ref.6

Subunit structure

Interacts with STX4. Interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion. Ref.6 Ref.7

Subcellular location

Cytoplasmcytosol By similarity. Cell membrane. Note: In platelets, predominantly cytosolic. Low amounts membrane-associated By similarity. Ref.6

Tissue specificity

Ubiquitously expressed in all tissues tested. Ref.2

Post-translational modification

Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4 By similarity.

Sequence similarities

Belongs to the STXBP/unc-18/SEC1 family.

Sequence caution

The sequence BAE41375.1 differs from that shown. Reason: Frameshift at position 512.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q60770-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q60770-2)

The sequence of this isoform differs from the canonical sequence as follows:
     344-370: Missing.
     484-511: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 592592Syntaxin-binding protein 3
PRO_0000206286

Regions

Region1 – 255255Mediates interaction with DOC2B

Natural variations

Alternative sequence344 – 37027Missing in isoform 2.
VSP_037061
Alternative sequence484 – 51128Missing in isoform 2.
VSP_037062

Experimental info

Sequence conflict1231K → R in BAE31079. Ref.3
Sequence conflict1661N → Y in BAE23717. Ref.3
Sequence conflict3031E → Q in BAE33068. Ref.3
Sequence conflict3611N → S in BAE33068. Ref.3
Sequence conflict4671E → G in BAE23717. Ref.3
Sequence conflict4891R → K in BAE31079. Ref.3

Secondary structure

................................................................................................ 592
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7874B71DE107871A

FASTA59267,942
        10         20         30         40         50         60 
MAPPVSERGL KSVVWRKIKT AVFDDCRKEG EWKIMLLDEF TTKLLSSCCK MTDLLEEGIT 

        70         80         90        100        110        120 
VIENIYKNRE PVRQMKALYF ISPTPKSVDC FLRDFGSKSE KKYKAAYIYF TDFCPDSLFN 

       130        140        150        160        170        180 
KIKASCSKSI RRCKEINISF IPQESQVYTL DVPDAFYYCY SPDPSNASRK EVVMEAMAEQ 

       190        200        210        220        230        240 
IVTVCATLDE NPGVRYKSKP LDNASKLAQL VEKKLEDYYK IDEKGLIKGK TQSQLLIIDR 

       250        260        270        280        290        300 
GFDPVSTVLH ELTFQAMAYD LLPIENDTYK YKTDGKEKEA VLEEDDDLWV RVRHRHIAVV 

       310        320        330        340        350        360 
LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ISKQVVHLNL AEDCMNKFKL 

       370        380        390        400        410        420 
NIEKLCKTEQ DLALGTDAEG QRVKDSMLVL LPVLLNKNHD NCDKIRAVLL YIFGINGTTE 

       430        440        450        460        470        480 
ENLDRLIHNV KIEDDSDMIR NWSHLGVPIV PPSQQAKPLR KDRSAEETFQ LSRWTPFIKD 

       490        500        510        520        530        540 
IMEDAIDNRL DSKEWPYCSR CPAVWNGSGA VSARQKPRTN YLELDRKNGS RLIIFVIGGI 

       550        560        570        580        590 
TYSEMRCAYE VSQAHKSCEV IIGSTHILTP RKLLDDIKML NKSKDKVSFK DE 

« Hide

Isoform 2 [UniParc].

Checksum: 0DBB266AF1E7D54C
Show »

FASTA53761,678

References

« Hide 'large scale' references
[1]"Molecular identification of two novel Munc-18 isoforms expressed in non-neuronal tissues."
Tellam J.T., McIntosh S., James D.E.
J. Biol. Chem. 270:5857-5863(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Adipocyte.
[2]"A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants."
Gengyo-Ando K., Kitayama H., Mukaida M., Ikawa Y.
J. Neurosci. 16:6695-6702(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and NOD.
Tissue: Bone marrow macrophage, Dendritic cell, Spinal cord, Thymocyte and Thymus.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[6]"Characterization of Munc-18c and syntaxin-4 in 3T3-L1 adipocytes. Putative role in insulin-dependent movement of GLUT-4."
Tellam J.T., Macaulay S.L., McIntosh S., Hewish D.R., Ward C.W., James D.E.
J. Biol. Chem. 272:6179-6186(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH STX4, SUBCELLULAR LOCATION.
[7]"Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis."
Ke B., Oh E., Thurmond D.C.
J. Biol. Chem. 282:21786-21797(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DOC2B AND STX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U19521 mRNA. Translation: AAA69913.1.
D30798 mRNA. Translation: BAA19478.1.
AK138609 mRNA. Translation: BAE23717.1.
AK151466 mRNA. Translation: BAE30424.1.
AK152261 mRNA. Translation: BAE31079.1.
AK155133 mRNA. Translation: BAE33068.1.
AK169799 mRNA. Translation: BAE41375.1. Frameshift.
AL671894, AL671917 Genomic DNA. Translation: CAM20601.1.
AL671917, AL671894 Genomic DNA. Translation: CAM17755.1.
BC062901 mRNA. Translation: AAH62901.1.
PIRI49239.
RefSeqNP_035634.1. NM_011504.1.
UniGeneMm.316894.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3PUKX-ray3.05A/B1-592[»]
ProteinModelPortalQ60770.
SMRQ60770. Positions 7-585.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203566. 1 interaction.
DIPDIP-60305N.
IntActQ60770. 1 interaction.
MINTMINT-4136068.
STRING10090.ENSMUSP00000099681.

PTM databases

PhosphoSiteQ60770.

Proteomic databases

PaxDbQ60770.
PRIDEQ60770.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102621; ENSMUSP00000099681; ENSMUSG00000027882. [Q60770-1]
GeneID20912.
KEGGmmu:20912.
UCSCuc008qzr.3. mouse. [Q60770-1]

Organism-specific databases

CTD20912.
MGIMGI:107362. Stxbp3a.

Phylogenomic databases

eggNOGCOG5158.
GeneTreeENSGT00390000005206.
HOGENOMHOG000232146.
HOVERGENHBG052710.
InParanoidQ3U2S2.
KOK15301.
OMAHQSECPA.
OrthoDBEOG78PV8M.
PhylomeDBQ60770.
TreeFamTF313242.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.

Gene expression databases

ArrayExpressQ60770.
BgeeQ60770.
CleanExMM_STXBP3A.
GenevestigatorQ60770.

Family and domain databases

Gene3D3.40.50.1910. 2 hits.
InterProIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERPTHR11679. PTHR11679. 1 hit.
PfamPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMSSF56815. SSF56815. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ60770.
NextBio299805.
PROQ60770.
SOURCESearch...

Entry information

Entry nameSTXB3_MOUSE
AccessionPrimary (citable) accession number: Q60770
Secondary accession number(s): Q3TE73 expand/collapse secondary AC list , Q3U2S2, Q3U8D9, Q3UA91, Q3UUB0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot