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Q60770

- STXB3_MOUSE

UniProt

Q60770 - STXB3_MOUSE

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Protein
Syntaxin-binding protein 3
Gene
Stxbp3, Stxbp3a, Unc18c
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.1 Publication

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. syntaxin binding Source: UniProtKB

GO - Biological processi

  1. brain development Source: MGI
  2. exocytosis Source: MGI
  3. glucose transport Source: MGI
  4. insulin secretion Source: MGI
  5. protein to membrane docking Source: MGI
  6. protein transport Source: UniProtKB-KW
  7. response to insulin Source: MGI
  8. vesicle docking involved in exocytosis Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.
REACT_215302. Disinhibition of SNARE formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-binding protein 3
Alternative name(s):
MUNC-18-3
Mammalian homolog of Unc-18c
Short name:
Munc-18c
Protein unc-18 homolog 3
Short name:
Unc18-3
Protein unc-18 homolog C
Short name:
Unc-18C
Gene namesi
Name:Stxbp3
Synonyms:Stxbp3a, Unc18c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:107362. Stxbp3a.

Subcellular locationi

Cytoplasmcytosol By similarity. Cell membrane
Note: In platelets, predominantly cytosolic. Low amounts membrane-associated By similarity.1 Publication

GO - Cellular componenti

  1. apical plasma membrane Source: MGI
  2. cytosol Source: MGI
  3. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 592592Syntaxin-binding protein 3
PRO_0000206286Add
BLAST

Post-translational modificationi

Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4 By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ60770.
PaxDbiQ60770.
PRIDEiQ60770.

PTM databases

PhosphoSiteiQ60770.

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues tested.1 Publication

Gene expression databases

ArrayExpressiQ60770.
BgeeiQ60770.
CleanExiMM_STXBP3A.
GenevestigatoriQ60770.

Interactioni

Subunit structurei

Interacts with STX4. Interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion.2 Publications

Protein-protein interaction databases

BioGridi203566. 1 interaction.
DIPiDIP-60305N.
IntActiQ60770. 1 interaction.
MINTiMINT-4136068.
STRINGi10090.ENSMUSP00000099681.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 2112
Helixi24 – 263
Beta strandi33 – 375
Helixi39 – 479
Helixi53 – 575
Beta strandi59 – 679
Beta strandi77 – 815
Helixi85 – 9511
Helixi97 – 993
Beta strandi106 – 1127
Helixi116 – 1249
Beta strandi128 – 1358
Beta strandi140 – 1445
Beta strandi147 – 1504
Helixi155 – 1595
Beta strandi163 – 1653
Helixi168 – 18821
Beta strandi193 – 1964
Beta strandi199 – 2024
Helixi203 – 21715
Beta strandi218 – 2214
Beta strandi232 – 2398
Helixi240 – 2423
Turni246 – 2483
Helixi254 – 2618
Helixi265 – 2673
Beta strandi268 – 2703
Beta strandi273 – 2764
Beta strandi279 – 2813
Beta strandi286 – 2883
Turni289 – 2946
Helixi297 – 3015
Turni309 – 3135
Helixi327 – 33610
Turni337 – 3393
Helixi340 – 36021
Helixi362 – 37312
Beta strandi374 – 3763
Helixi387 – 3904
Turni391 – 3944
Helixi401 – 41414
Helixi420 – 42910
Turni434 – 4363
Helixi437 – 4404
Helixi441 – 4455
Beta strandi449 – 4557
Helixi468 – 4703
Helixi479 – 4868
Turni492 – 4943
Beta strandi530 – 5378
Helixi542 – 55413
Beta strandi557 – 56812
Helixi570 – 5778

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PUKX-ray3.05A/B1-592[»]
ProteinModelPortaliQ60770.
SMRiQ60770. Positions 7-585.

Miscellaneous databases

EvolutionaryTraceiQ60770.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 255255Mediates interaction with DOC2B
Add
BLAST

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.

Phylogenomic databases

eggNOGiCOG5158.
GeneTreeiENSGT00390000005206.
HOGENOMiHOG000232146.
HOVERGENiHBG052710.
InParanoidiQ3U2S2.
KOiK15301.
OMAiMSDLMAE.
OrthoDBiEOG78PV8M.
PhylomeDBiQ60770.
TreeFamiTF313242.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q60770-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAPPVSERGL KSVVWRKIKT AVFDDCRKEG EWKIMLLDEF TTKLLSSCCK    50
MTDLLEEGIT VIENIYKNRE PVRQMKALYF ISPTPKSVDC FLRDFGSKSE 100
KKYKAAYIYF TDFCPDSLFN KIKASCSKSI RRCKEINISF IPQESQVYTL 150
DVPDAFYYCY SPDPSNASRK EVVMEAMAEQ IVTVCATLDE NPGVRYKSKP 200
LDNASKLAQL VEKKLEDYYK IDEKGLIKGK TQSQLLIIDR GFDPVSTVLH 250
ELTFQAMAYD LLPIENDTYK YKTDGKEKEA VLEEDDDLWV RVRHRHIAVV 300
LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ISKQVVHLNL 350
AEDCMNKFKL NIEKLCKTEQ DLALGTDAEG QRVKDSMLVL LPVLLNKNHD 400
NCDKIRAVLL YIFGINGTTE ENLDRLIHNV KIEDDSDMIR NWSHLGVPIV 450
PPSQQAKPLR KDRSAEETFQ LSRWTPFIKD IMEDAIDNRL DSKEWPYCSR 500
CPAVWNGSGA VSARQKPRTN YLELDRKNGS RLIIFVIGGI TYSEMRCAYE 550
VSQAHKSCEV IIGSTHILTP RKLLDDIKML NKSKDKVSFK DE 592
Length:592
Mass (Da):67,942
Last modified:November 1, 1996 - v1
Checksum:i7874B71DE107871A
GO
Isoform 2 (identifier: Q60770-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     344-370: Missing.
     484-511: Missing.

Note: No experimental confirmation available.

Show »
Length:537
Mass (Da):61,678
Checksum:i0DBB266AF1E7D54C
GO

Sequence cautioni

The sequence BAE41375.1 differs from that shown. Reason: Frameshift at position 512.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei344 – 37027Missing in isoform 2.
VSP_037061Add
BLAST
Alternative sequencei484 – 51128Missing in isoform 2.
VSP_037062Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti123 – 1231K → R in BAE31079. 1 Publication
Sequence conflicti166 – 1661N → Y in BAE23717. 1 Publication
Sequence conflicti303 – 3031E → Q in BAE33068. 1 Publication
Sequence conflicti361 – 3611N → S in BAE33068. 1 Publication
Sequence conflicti467 – 4671E → G in BAE23717. 1 Publication
Sequence conflicti489 – 4891R → K in BAE31079. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U19521 mRNA. Translation: AAA69913.1.
D30798 mRNA. Translation: BAA19478.1.
AK138609 mRNA. Translation: BAE23717.1.
AK151466 mRNA. Translation: BAE30424.1.
AK152261 mRNA. Translation: BAE31079.1.
AK155133 mRNA. Translation: BAE33068.1.
AK169799 mRNA. Translation: BAE41375.1. Frameshift.
AL671894, AL671917 Genomic DNA. Translation: CAM20601.1.
AL671917, AL671894 Genomic DNA. Translation: CAM17755.1.
BC062901 mRNA. Translation: AAH62901.1.
CCDSiCCDS17768.1. [Q60770-1]
PIRiI49239.
RefSeqiNP_035634.1. NM_011504.1. [Q60770-1]
UniGeneiMm.316894.

Genome annotation databases

EnsembliENSMUST00000102621; ENSMUSP00000099681; ENSMUSG00000027882. [Q60770-1]
GeneIDi20912.
KEGGimmu:20912.
UCSCiuc008qzr.3. mouse. [Q60770-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U19521 mRNA. Translation: AAA69913.1 .
D30798 mRNA. Translation: BAA19478.1 .
AK138609 mRNA. Translation: BAE23717.1 .
AK151466 mRNA. Translation: BAE30424.1 .
AK152261 mRNA. Translation: BAE31079.1 .
AK155133 mRNA. Translation: BAE33068.1 .
AK169799 mRNA. Translation: BAE41375.1 . Frameshift.
AL671894 , AL671917 Genomic DNA. Translation: CAM20601.1 .
AL671917 , AL671894 Genomic DNA. Translation: CAM17755.1 .
BC062901 mRNA. Translation: AAH62901.1 .
CCDSi CCDS17768.1. [Q60770-1 ]
PIRi I49239.
RefSeqi NP_035634.1. NM_011504.1. [Q60770-1 ]
UniGenei Mm.316894.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3PUK X-ray 3.05 A/B 1-592 [» ]
ProteinModelPortali Q60770.
SMRi Q60770. Positions 7-585.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203566. 1 interaction.
DIPi DIP-60305N.
IntActi Q60770. 1 interaction.
MINTi MINT-4136068.
STRINGi 10090.ENSMUSP00000099681.

PTM databases

PhosphoSitei Q60770.

Proteomic databases

MaxQBi Q60770.
PaxDbi Q60770.
PRIDEi Q60770.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102621 ; ENSMUSP00000099681 ; ENSMUSG00000027882 . [Q60770-1 ]
GeneIDi 20912.
KEGGi mmu:20912.
UCSCi uc008qzr.3. mouse. [Q60770-1 ]

Organism-specific databases

CTDi 20912.
MGIi MGI:107362. Stxbp3a.

Phylogenomic databases

eggNOGi COG5158.
GeneTreei ENSGT00390000005206.
HOGENOMi HOG000232146.
HOVERGENi HBG052710.
InParanoidi Q3U2S2.
KOi K15301.
OMAi MSDLMAE.
OrthoDBi EOG78PV8M.
PhylomeDBi Q60770.
TreeFami TF313242.

Enzyme and pathway databases

Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.
REACT_215302. Disinhibition of SNARE formation.

Miscellaneous databases

EvolutionaryTracei Q60770.
NextBioi 299805.
PROi Q60770.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q60770.
Bgeei Q60770.
CleanExi MM_STXBP3A.
Genevestigatori Q60770.

Family and domain databases

Gene3Di 3.40.50.1910. 2 hits.
InterProi IPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view ]
PANTHERi PTHR11679. PTHR11679. 1 hit.
Pfami PF00995. Sec1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMi SSF56815. SSF56815. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular identification of two novel Munc-18 isoforms expressed in non-neuronal tissues."
    Tellam J.T., McIntosh S., James D.E.
    J. Biol. Chem. 270:5857-5863(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Adipocyte.
  2. "A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants."
    Gengyo-Ando K., Kitayama H., Mukaida M., Ikawa Y.
    J. Neurosci. 16:6695-6702(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow macrophage, Dendritic cell, Spinal cord, Thymocyte and Thymus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  6. "Characterization of Munc-18c and syntaxin-4 in 3T3-L1 adipocytes. Putative role in insulin-dependent movement of GLUT-4."
    Tellam J.T., Macaulay S.L., McIntosh S., Hewish D.R., Ward C.W., James D.E.
    J. Biol. Chem. 272:6179-6186(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH STX4, SUBCELLULAR LOCATION.
  7. "Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis."
    Ke B., Oh E., Thurmond D.C.
    J. Biol. Chem. 282:21786-21797(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DOC2B AND STX4.

Entry informationi

Entry nameiSTXB3_MOUSE
AccessioniPrimary (citable) accession number: Q60770
Secondary accession number(s): Q3TE73
, Q3U2S2, Q3U8D9, Q3UA91, Q3UUB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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