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Q60770

- STXB3_MOUSE

UniProt

Q60770 - STXB3_MOUSE

Protein

Syntaxin-binding protein 3

Gene

Stxbp3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. syntaxin binding Source: UniProtKB

    GO - Biological processi

    1. brain development Source: MGI
    2. exocytosis Source: MGI
    3. glucose transport Source: MGI
    4. insulin secretion Source: MGI
    5. protein to membrane docking Source: MGI
    6. protein transport Source: UniProtKB-KW
    7. response to insulin Source: MGI
    8. vesicle docking involved in exocytosis Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_215302. Disinhibition of SNARE formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Syntaxin-binding protein 3
    Alternative name(s):
    MUNC-18-3
    Mammalian homolog of Unc-18c
    Short name:
    Munc-18c
    Protein unc-18 homolog 3
    Short name:
    Unc18-3
    Protein unc-18 homolog C
    Short name:
    Unc-18C
    Gene namesi
    Name:Stxbp3
    Synonyms:Stxbp3a, Unc18c
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:107362. Stxbp3a.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Cell membrane 1 Publication
    Note: In platelets, predominantly cytosolic. Low amounts membrane-associated By similarity.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: MGI
    2. cytosol Source: MGI
    3. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 592592Syntaxin-binding protein 3PRO_0000206286Add
    BLAST

    Post-translational modificationi

    Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ60770.
    PaxDbiQ60770.
    PRIDEiQ60770.

    PTM databases

    PhosphoSiteiQ60770.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed in all tissues tested.1 Publication

    Gene expression databases

    ArrayExpressiQ60770.
    BgeeiQ60770.
    CleanExiMM_STXBP3A.
    GenevestigatoriQ60770.

    Interactioni

    Subunit structurei

    Interacts with STX4. Interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion.2 Publications

    Protein-protein interaction databases

    BioGridi203566. 1 interaction.
    DIPiDIP-60305N.
    IntActiQ60770. 1 interaction.
    MINTiMINT-4136068.
    STRINGi10090.ENSMUSP00000099681.

    Structurei

    Secondary structure

    1
    592
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 2112
    Helixi24 – 263
    Beta strandi33 – 375
    Helixi39 – 479
    Helixi53 – 575
    Beta strandi59 – 679
    Beta strandi77 – 815
    Helixi85 – 9511
    Helixi97 – 993
    Beta strandi106 – 1127
    Helixi116 – 1249
    Beta strandi128 – 1358
    Beta strandi140 – 1445
    Beta strandi147 – 1504
    Helixi155 – 1595
    Beta strandi163 – 1653
    Helixi168 – 18821
    Beta strandi193 – 1964
    Beta strandi199 – 2024
    Helixi203 – 21715
    Beta strandi218 – 2214
    Beta strandi232 – 2398
    Helixi240 – 2423
    Turni246 – 2483
    Helixi254 – 2618
    Helixi265 – 2673
    Beta strandi268 – 2703
    Beta strandi273 – 2764
    Beta strandi279 – 2813
    Beta strandi286 – 2883
    Turni289 – 2946
    Helixi297 – 3015
    Turni309 – 3135
    Helixi327 – 33610
    Turni337 – 3393
    Helixi340 – 36021
    Helixi362 – 37312
    Beta strandi374 – 3763
    Helixi387 – 3904
    Turni391 – 3944
    Helixi401 – 41414
    Helixi420 – 42910
    Turni434 – 4363
    Helixi437 – 4404
    Helixi441 – 4455
    Beta strandi449 – 4557
    Helixi468 – 4703
    Helixi479 – 4868
    Turni492 – 4943
    Beta strandi530 – 5378
    Helixi542 – 55413
    Beta strandi557 – 56812
    Helixi570 – 5778

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3PUKX-ray3.05A/B1-592[»]
    ProteinModelPortaliQ60770.
    SMRiQ60770. Positions 7-585.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ60770.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 255255Mediates interaction with DOC2BAdd
    BLAST

    Sequence similaritiesi

    Belongs to the STXBP/unc-18/SEC1 family.Curated

    Phylogenomic databases

    eggNOGiCOG5158.
    GeneTreeiENSGT00390000005206.
    HOGENOMiHOG000232146.
    HOVERGENiHBG052710.
    InParanoidiQ3U2S2.
    KOiK15301.
    OMAiMSDLMAE.
    OrthoDBiEOG78PV8M.
    PhylomeDBiQ60770.
    TreeFamiTF313242.

    Family and domain databases

    Gene3Di3.40.50.1910. 2 hits.
    InterProiIPR027482. Sec-1-like_dom2.
    IPR001619. Sec1-like.
    [Graphical view]
    PANTHERiPTHR11679. PTHR11679. 1 hit.
    PfamiPF00995. Sec1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
    SUPFAMiSSF56815. SSF56815. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q60770-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAPPVSERGL KSVVWRKIKT AVFDDCRKEG EWKIMLLDEF TTKLLSSCCK    50
    MTDLLEEGIT VIENIYKNRE PVRQMKALYF ISPTPKSVDC FLRDFGSKSE 100
    KKYKAAYIYF TDFCPDSLFN KIKASCSKSI RRCKEINISF IPQESQVYTL 150
    DVPDAFYYCY SPDPSNASRK EVVMEAMAEQ IVTVCATLDE NPGVRYKSKP 200
    LDNASKLAQL VEKKLEDYYK IDEKGLIKGK TQSQLLIIDR GFDPVSTVLH 250
    ELTFQAMAYD LLPIENDTYK YKTDGKEKEA VLEEDDDLWV RVRHRHIAVV 300
    LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ISKQVVHLNL 350
    AEDCMNKFKL NIEKLCKTEQ DLALGTDAEG QRVKDSMLVL LPVLLNKNHD 400
    NCDKIRAVLL YIFGINGTTE ENLDRLIHNV KIEDDSDMIR NWSHLGVPIV 450
    PPSQQAKPLR KDRSAEETFQ LSRWTPFIKD IMEDAIDNRL DSKEWPYCSR 500
    CPAVWNGSGA VSARQKPRTN YLELDRKNGS RLIIFVIGGI TYSEMRCAYE 550
    VSQAHKSCEV IIGSTHILTP RKLLDDIKML NKSKDKVSFK DE 592
    Length:592
    Mass (Da):67,942
    Last modified:November 1, 1996 - v1
    Checksum:i7874B71DE107871A
    GO
    Isoform 2 (identifier: Q60770-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         344-370: Missing.
         484-511: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:537
    Mass (Da):61,678
    Checksum:i0DBB266AF1E7D54C
    GO

    Sequence cautioni

    The sequence BAE41375.1 differs from that shown. Reason: Frameshift at position 512.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti123 – 1231K → R in BAE31079. (PubMed:16141072)Curated
    Sequence conflicti166 – 1661N → Y in BAE23717. (PubMed:16141072)Curated
    Sequence conflicti303 – 3031E → Q in BAE33068. (PubMed:16141072)Curated
    Sequence conflicti361 – 3611N → S in BAE33068. (PubMed:16141072)Curated
    Sequence conflicti467 – 4671E → G in BAE23717. (PubMed:16141072)Curated
    Sequence conflicti489 – 4891R → K in BAE31079. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei344 – 37027Missing in isoform 2. 1 PublicationVSP_037061Add
    BLAST
    Alternative sequencei484 – 51128Missing in isoform 2. 1 PublicationVSP_037062Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19521 mRNA. Translation: AAA69913.1.
    D30798 mRNA. Translation: BAA19478.1.
    AK138609 mRNA. Translation: BAE23717.1.
    AK151466 mRNA. Translation: BAE30424.1.
    AK152261 mRNA. Translation: BAE31079.1.
    AK155133 mRNA. Translation: BAE33068.1.
    AK169799 mRNA. Translation: BAE41375.1. Frameshift.
    AL671894, AL671917 Genomic DNA. Translation: CAM20601.1.
    AL671917, AL671894 Genomic DNA. Translation: CAM17755.1.
    BC062901 mRNA. Translation: AAH62901.1.
    CCDSiCCDS17768.1. [Q60770-1]
    PIRiI49239.
    RefSeqiNP_035634.1. NM_011504.1. [Q60770-1]
    UniGeneiMm.316894.

    Genome annotation databases

    EnsembliENSMUST00000102621; ENSMUSP00000099681; ENSMUSG00000027882. [Q60770-1]
    GeneIDi20912.
    KEGGimmu:20912.
    UCSCiuc008qzr.3. mouse. [Q60770-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19521 mRNA. Translation: AAA69913.1 .
    D30798 mRNA. Translation: BAA19478.1 .
    AK138609 mRNA. Translation: BAE23717.1 .
    AK151466 mRNA. Translation: BAE30424.1 .
    AK152261 mRNA. Translation: BAE31079.1 .
    AK155133 mRNA. Translation: BAE33068.1 .
    AK169799 mRNA. Translation: BAE41375.1 . Frameshift.
    AL671894 , AL671917 Genomic DNA. Translation: CAM20601.1 .
    AL671917 , AL671894 Genomic DNA. Translation: CAM17755.1 .
    BC062901 mRNA. Translation: AAH62901.1 .
    CCDSi CCDS17768.1. [Q60770-1 ]
    PIRi I49239.
    RefSeqi NP_035634.1. NM_011504.1. [Q60770-1 ]
    UniGenei Mm.316894.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3PUK X-ray 3.05 A/B 1-592 [» ]
    ProteinModelPortali Q60770.
    SMRi Q60770. Positions 7-585.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203566. 1 interaction.
    DIPi DIP-60305N.
    IntActi Q60770. 1 interaction.
    MINTi MINT-4136068.
    STRINGi 10090.ENSMUSP00000099681.

    PTM databases

    PhosphoSitei Q60770.

    Proteomic databases

    MaxQBi Q60770.
    PaxDbi Q60770.
    PRIDEi Q60770.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102621 ; ENSMUSP00000099681 ; ENSMUSG00000027882 . [Q60770-1 ]
    GeneIDi 20912.
    KEGGi mmu:20912.
    UCSCi uc008qzr.3. mouse. [Q60770-1 ]

    Organism-specific databases

    CTDi 20912.
    MGIi MGI:107362. Stxbp3a.

    Phylogenomic databases

    eggNOGi COG5158.
    GeneTreei ENSGT00390000005206.
    HOGENOMi HOG000232146.
    HOVERGENi HBG052710.
    InParanoidi Q3U2S2.
    KOi K15301.
    OMAi MSDLMAE.
    OrthoDBi EOG78PV8M.
    PhylomeDBi Q60770.
    TreeFami TF313242.

    Enzyme and pathway databases

    Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_215302. Disinhibition of SNARE formation.

    Miscellaneous databases

    EvolutionaryTracei Q60770.
    NextBioi 299805.
    PROi Q60770.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q60770.
    Bgeei Q60770.
    CleanExi MM_STXBP3A.
    Genevestigatori Q60770.

    Family and domain databases

    Gene3Di 3.40.50.1910. 2 hits.
    InterProi IPR027482. Sec-1-like_dom2.
    IPR001619. Sec1-like.
    [Graphical view ]
    PANTHERi PTHR11679. PTHR11679. 1 hit.
    Pfami PF00995. Sec1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005715. VPS45_Sec1. 1 hit.
    SUPFAMi SSF56815. SSF56815. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular identification of two novel Munc-18 isoforms expressed in non-neuronal tissues."
      Tellam J.T., McIntosh S., James D.E.
      J. Biol. Chem. 270:5857-5863(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Adipocyte.
    2. "A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants."
      Gengyo-Ando K., Kitayama H., Mukaida M., Ikawa Y.
      J. Neurosci. 16:6695-6702(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow macrophage, Dendritic cell, Spinal cord, Thymocyte and Thymus.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    6. "Characterization of Munc-18c and syntaxin-4 in 3T3-L1 adipocytes. Putative role in insulin-dependent movement of GLUT-4."
      Tellam J.T., Macaulay S.L., McIntosh S., Hewish D.R., Ward C.W., James D.E.
      J. Biol. Chem. 272:6179-6186(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH STX4, SUBCELLULAR LOCATION.
    7. "Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis."
      Ke B., Oh E., Thurmond D.C.
      J. Biol. Chem. 282:21786-21797(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DOC2B AND STX4.

    Entry informationi

    Entry nameiSTXB3_MOUSE
    AccessioniPrimary (citable) accession number: Q60770
    Secondary accession number(s): Q3TE73
    , Q3U2S2, Q3U8D9, Q3UA91, Q3UUB0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3