Q60770 (STXB3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-binding protein 3

Alternative name(s):
MUNC-18-3
Mammalian homolog of Unc-18c
Short name=Munc-18c
Protein unc-18 homolog 3
Short name=Unc18-3
Protein unc-18 homolog C
Short name=Unc-18C

Gene names

Name:	Stxbp3
Synonyms:	Stxbp3a, Unc18c

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. Ref.6
Subunit structure	Interacts with STX4. Interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion. Ref.6 Ref.7
Subcellular location	Cytoplasm › cytosol By similarity. Cell membrane. Note: In platelets, predominantly cytosolic. Low amounts membrane-associated By similarity. Ref.6
Tissue specificity	Ubiquitously expressed in all tissues tested. Ref.2
Post-translational modification	Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4 By similarity.
Sequence similarities	Belongs to the STXBP/unc-18/SEC1 family.
Sequence caution	The sequence BAE41375.1 differs from that shown. Reason: Frameshift at position 512.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from mutant phenotype PubMed 15690082. Source: MGI exocytosis Inferred from mutant phenotype PubMed 19188424. Source: MGI glucose transport Inferred from direct assay PubMed 12297296. Source: MGI insulin secretion Inferred from mutant phenotype PubMed 19188424. Source: MGI protein to membrane docking Inferred from mutant phenotype PubMed 19188424. Source: MGI protein transport Inferred from electronic annotation. Source: UniProtKB-KW response to insulin stimulus Inferred from mutant phenotype PubMed 15690082. Source: MGI vesicle docking involved in exocytosis Inferred from electronic annotation. Source: InterPro
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 18505797. Source: MGI cytosol Inferred from direct assay PubMed 12297296PubMed 12832401. Source: MGI
Molecular_function	syntaxin binding Inferred from direct assay PubMed 12297296. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q60770-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q60770-2)

The sequence of this isoform differs from the canonical sequence as follows:
     344-370: Missing.
     484-511: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 592

592

Syntaxin-binding protein 3

PRO_0000206286

Regions

Region

1 – 255

255

Mediates interaction with DOC2B

Amino acid modifications

Modified residue

198

1

Phosphoserine Ref.8

Natural variations

Alternative sequence

344 – 370

27

Missing in isoform 2.

VSP_037061

Alternative sequence

484 – 511

28

Missing in isoform 2.

VSP_037062

Experimental info

Sequence conflict

123

1

K → R in BAE31079. Ref.3

Sequence conflict

166

1

N → Y in BAE23717. Ref.3

Sequence conflict

303

1

E → Q in BAE33068. Ref.3

Sequence conflict

361

1

N → S in BAE33068. Ref.3

Sequence conflict

467

1

E → G in BAE23717. Ref.3

Sequence conflict

489

1

R → K in BAE31079. Ref.3

Secondary structure

1

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592

Helix Strand Turn

Details...

Helix

10 – 21

12

Helix

24 – 26

3

Beta strand

33 – 37

5

Helix

39 – 47

9

Helix

53 – 57

5

Beta strand

59 – 67

9

Beta strand

77 – 81

5

Helix

85 – 95

11

Helix

97 – 99

3

Beta strand

102 – 104

3

Beta strand

106 – 112

7

Helix

116 – 124

9

Beta strand

128 – 135

8

Beta strand

140 – 144

5

Beta strand

147 – 150

4

Helix

155 – 159

5

Beta strand

163 – 165

3

Helix

168 – 188

21

Beta strand

193 – 196

4

Beta strand

199 – 202

4

Helix

203 – 217

15

Beta strand

218 – 221

4

Beta strand

232 – 239

8

Helix

240 – 242

3

Turn

246 – 248

3

Helix

254 – 261

8

Helix

265 – 267

3

Beta strand

268 – 270

3

Beta strand

273 – 276

4

Beta strand

279 – 281

3

Beta strand

286 – 288

3

Turn

289 – 294

6

Helix

297 – 301

5

Turn

309 – 313

5

Helix

327 – 336

10

Turn

337 – 339

3

Helix

340 – 360

21

Helix

362 – 373

12

Beta strand

374 – 376

3

Beta strand

384 – 386

3

Helix

387 – 390

4

Turn

391 – 394

4

Helix

401 – 414

14

Helix

420 – 429

10

Turn

434 – 436

3

Helix

437 – 440

4

Helix

441 – 445

5

Beta strand

449 – 455

7

Helix

468 – 470

3

Helix

479 – 486

8

Turn

492 – 494

3

Beta strand

530 – 537

8

Helix

542 – 554

13

Beta strand

557 – 568

12

Helix

570 – 577

8

Beta strand

578 – 581

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7874B71DE107871A	FASTA	592	67,942
	10 20 30 40 50 60 MAPPVSERGL KSVVWRKIKT AVFDDCRKEG EWKIMLLDEF TTKLLSSCCK MTDLLEEGIT 70 80 90 100 110 120 VIENIYKNRE PVRQMKALYF ISPTPKSVDC FLRDFGSKSE KKYKAAYIYF TDFCPDSLFN 130 140 150 160 170 180 KIKASCSKSI RRCKEINISF IPQESQVYTL DVPDAFYYCY SPDPSNASRK EVVMEAMAEQ 190 200 210 220 230 240 IVTVCATLDE NPGVRYKSKP LDNASKLAQL VEKKLEDYYK IDEKGLIKGK TQSQLLIIDR 250 260 270 280 290 300 GFDPVSTVLH ELTFQAMAYD LLPIENDTYK YKTDGKEKEA VLEEDDDLWV RVRHRHIAVV 310 320 330 340 350 360 LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ISKQVVHLNL AEDCMNKFKL 370 380 390 400 410 420 NIEKLCKTEQ DLALGTDAEG QRVKDSMLVL LPVLLNKNHD NCDKIRAVLL YIFGINGTTE 430 440 450 460 470 480 ENLDRLIHNV KIEDDSDMIR NWSHLGVPIV PPSQQAKPLR KDRSAEETFQ LSRWTPFIKD 490 500 510 520 530 540 IMEDAIDNRL DSKEWPYCSR CPAVWNGSGA VSARQKPRTN YLELDRKNGS RLIIFVIGGI 550 560 570 580 590 TYSEMRCAYE VSQAHKSCEV IIGSTHILTP RKLLDDIKML NKSKDKVSFK DE
	Isoform 2 [UniParc]. Checksum: 0DBB266AF1E7D54C	FASTA	537	61,678
	10 20 30 40 50 60 MAPPVSERGL KSVVWRKIKT AVFDDCRKEG EWKIMLLDEF TTKLLSSCCK MTDLLEEGIT 70 80 90 100 110 120 VIENIYKNRE PVRQMKALYF ISPTPKSVDC FLRDFGSKSE KKYKAAYIYF TDFCPDSLFN 130 140 150 160 170 180 KIKASCSKSI RRCKEINISF IPQESQVYTL DVPDAFYYCY SPDPSNASRK EVVMEAMAEQ 190 200 210 220 230 240 IVTVCATLDE NPGVRYKSKP LDNASKLAQL VEKKLEDYYK IDEKGLIKGK TQSQLLIIDR 250 260 270 280 290 300 GFDPVSTVLH ELTFQAMAYD LLPIENDTYK YKTDGKEKEA VLEEDDDLWV RVRHRHIAVV 310 320 330 340 350 360 LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ISKDLALGTD AEGQRVKDSM 370 380 390 400 410 420 LVLLPVLLNK NHDNCDKIRA VLLYIFGING TTEENLDRLI HNVKIEDDSD MIRNWSHLGV 430 440 450 460 470 480 PIVPPSQQAK PLRKDRSAEE TFQLSRWTPF IKDIMESARQ KPRTNYLELD RKNGSRLIIF 490 500 510 520 530 VIGGITYSEM RCAYEVSQAH KSCEVIIGST HILTPRKLLD DIKMLNKSKD KVSFKDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular identification of two novel Munc-18 isoforms expressed in non-neuronal tissues." Tellam J.T., McIntosh S., James D.E. J. Biol. Chem. 270:5857-5863(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Adipocyte.
[2]	"A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants." Gengyo-Ando K., Kitayama H., Mukaida M., Ikawa Y. J. Neurosci. 16:6695-6702(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and NOD. Tissue: Bone marrow macrophage, Dendritic cell, Spinal cord, Thymocyte and Thymus.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[6]	"Characterization of Munc-18c and syntaxin-4 in 3T3-L1 adipocytes. Putative role in insulin-dependent movement of GLUT-4." Tellam J.T., Macaulay S.L., McIntosh S., Hewish D.R., Ward C.W., James D.E. J. Biol. Chem. 272:6179-6186(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH STX4, SUBCELLULAR LOCATION.
[7]	"Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis." Ke B., Oh E., Thurmond D.C. J. Biol. Chem. 282:21786-21797(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DOC2B AND STX4.
[8]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U19521 mRNA. Translation: AAA69913.1.
D30798 mRNA. Translation: BAA19478.1.
AK138609 mRNA. Translation: BAE23717.1.
AK151466 mRNA. Translation: BAE30424.1.
AK152261 mRNA. Translation: BAE31079.1.
AK155133 mRNA. Translation: BAE33068.1.
AK169799 mRNA. Translation: BAE41375.1. Frameshift.
AL671894, AL671917 Genomic DNA. Translation: CAM20601.1.
AL671917, AL671894 Genomic DNA. Translation: CAM17755.1.
BC062901 mRNA. Translation: AAH62901.1.

IPI

IPI00624098.
IPI00652134.

PIR

I49239.

RefSeq

NP_035634.1. NM_011504.1.

UniGene

Mm.316894.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PJX	X-ray	3.15	A/B	1-592	[»]
3PUK	X-ray	3.05	A/B	1-592	[»]

ProteinModelPortal

Q60770.

SMR

Q60770. Positions 7-585.

ModBase

Search...

Protein-protein interaction databases

STRING

10090.ENSMUSP00000099681.

PTM databases

PhosphoSite

Q60770.

Proteomic databases

PaxDb

Q60770.

PRIDE

Q60770.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000102621; ENSMUSP00000099681; ENSMUSG00000027882.

GeneID

20912.

KEGG

mmu:20912.

UCSC

uc008qzr.3. mouse.

Organism-specific databases

CTD

20912.

MGI

MGI:107362. Stxbp3a.

Phylogenomic databases

eggNOG

COG5158.

GeneTree

ENSGT00390000005206.

HOGENOM

HOG000232146.

HOVERGEN

HBG052710.

InParanoid

Q3U2S2.

KO

K15301.

OMA

HQSECPA.

OrthoDB

EOG44J2HV.

Enzyme and pathway databases

Reactome

REACT_147847. Translocation of Glut4 to the Plasma Membrane.
REACT_88307. Membrane Trafficking.

Gene expression databases

ArrayExpress

Q60770.

Bgee

Q60770.

CleanEx

MM_STXBP3A.

Genevestigator

Q60770.

GermOnline

ENSMUSG00000027882. Mus musculus.

Family and domain databases

InterPro

IPR001619. Sec1-like.
[Graphical view]

PANTHER

PTHR11679. PTHR11679. 1 hit.

Pfam

PF00995. Sec1. 1 hit.
[Graphical view]

PIRSF

PIRSF005715. VPS45_Sec1. 1 hit.

SUPFAM

SSF56815. Sec1-like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q60770.

NextBio

299805.

SOURCE

Search...

Entry information

Entry name

STXB3_MOUSE

Accession

Primary (citable) accession number: Q60770
Secondary accession number(s): Q3TE73 , Q3U2S2, Q3U8D9, Q3UA91, Q3UUB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program