Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Syntaxin-binding protein 3

Gene

Stxbp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.1 Publication

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • brain development Source: MGI
  • exocytosis Source: MGI
  • glucose transport Source: MGI
  • insulin secretion Source: MGI
  • membrane organization Source: Reactome
  • negative regulation of calcium ion-dependent exocytosis Source: MGI
  • neutrophil degranulation Source: Ensembl
  • platelet aggregation Source: MGI
  • protein heterooligomerization Source: Ensembl
  • protein to membrane docking Source: MGI
  • response to insulin Source: MGI
  • vesicle docking involved in exocytosis Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-114516. Disinhibition of SNARE formation.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-binding protein 3
Alternative name(s):
MUNC-18-3
Mammalian homolog of Unc-18c
Short name:
Munc-18c
Protein unc-18 homolog 3
Short name:
Unc18-3
Protein unc-18 homolog C
Short name:
Unc-18C
Gene namesi
Name:Stxbp3
Synonyms:Stxbp3a, Unc18c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:107362. Stxbp3.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • basolateral plasma membrane Source: Ensembl
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • plasma membrane Source: MGI
  • platelet alpha granule Source: MGI
  • specific granule Source: MGI
  • tertiary granule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002062861 – 592Syntaxin-binding protein 3Add BLAST592

Post-translational modificationi

Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ60770.
PaxDbiQ60770.
PeptideAtlasiQ60770.
PRIDEiQ60770.

PTM databases

iPTMnetiQ60770.
PhosphoSitePlusiQ60770.
SwissPalmiQ60770.

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues tested.1 Publication

Gene expression databases

BgeeiENSMUSG00000027882.
CleanExiMM_STXBP3A.
ExpressionAtlasiQ60770. baseline and differential.
GenevisibleiQ60770. MM.

Interactioni

Subunit structurei

Interacts with STX4. Interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion.2 Publications

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi203566. 1 interactor.
DIPiDIP-60305N.
IntActiQ60770. 7 interactors.
MINTiMINT-4136068.
STRINGi10090.ENSMUSP00000099681.

Structurei

Secondary structure

1592
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 21Combined sources12
Helixi24 – 26Combined sources3
Beta strandi33 – 37Combined sources5
Helixi39 – 47Combined sources9
Helixi53 – 57Combined sources5
Beta strandi59 – 67Combined sources9
Beta strandi77 – 81Combined sources5
Helixi85 – 95Combined sources11
Helixi97 – 99Combined sources3
Beta strandi106 – 112Combined sources7
Helixi116 – 124Combined sources9
Beta strandi128 – 135Combined sources8
Beta strandi140 – 144Combined sources5
Beta strandi147 – 150Combined sources4
Helixi155 – 159Combined sources5
Beta strandi163 – 165Combined sources3
Helixi168 – 188Combined sources21
Beta strandi193 – 196Combined sources4
Beta strandi199 – 202Combined sources4
Helixi203 – 217Combined sources15
Beta strandi218 – 221Combined sources4
Beta strandi232 – 239Combined sources8
Helixi240 – 242Combined sources3
Turni246 – 248Combined sources3
Helixi254 – 261Combined sources8
Helixi265 – 267Combined sources3
Beta strandi268 – 270Combined sources3
Beta strandi273 – 276Combined sources4
Beta strandi279 – 281Combined sources3
Beta strandi286 – 288Combined sources3
Turni289 – 294Combined sources6
Helixi297 – 301Combined sources5
Turni309 – 313Combined sources5
Helixi327 – 336Combined sources10
Turni337 – 339Combined sources3
Helixi340 – 360Combined sources21
Helixi362 – 373Combined sources12
Beta strandi374 – 376Combined sources3
Helixi387 – 390Combined sources4
Turni391 – 394Combined sources4
Helixi401 – 414Combined sources14
Helixi420 – 429Combined sources10
Turni434 – 436Combined sources3
Helixi437 – 440Combined sources4
Helixi441 – 445Combined sources5
Beta strandi449 – 455Combined sources7
Helixi468 – 470Combined sources3
Helixi479 – 486Combined sources8
Turni492 – 494Combined sources3
Beta strandi530 – 537Combined sources8
Helixi542 – 554Combined sources13
Beta strandi557 – 568Combined sources12
Helixi570 – 577Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PUKX-ray3.05A/B1-592[»]
ProteinModelPortaliQ60770.
SMRiQ60770.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60770.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 255Mediates interaction with DOC2B1 PublicationAdd BLAST255

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.Curated

Phylogenomic databases

eggNOGiKOG1300. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00390000005206.
HOGENOMiHOG000232146.
HOVERGENiHBG052710.
InParanoidiQ60770.
KOiK15301.
OMAiRKDRSTE.
OrthoDBiEOG091G0WUI.
PhylomeDBiQ60770.
TreeFamiTF313242.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60770-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPPVSERGL KSVVWRKIKT AVFDDCRKEG EWKIMLLDEF TTKLLSSCCK
60 70 80 90 100
MTDLLEEGIT VIENIYKNRE PVRQMKALYF ISPTPKSVDC FLRDFGSKSE
110 120 130 140 150
KKYKAAYIYF TDFCPDSLFN KIKASCSKSI RRCKEINISF IPQESQVYTL
160 170 180 190 200
DVPDAFYYCY SPDPSNASRK EVVMEAMAEQ IVTVCATLDE NPGVRYKSKP
210 220 230 240 250
LDNASKLAQL VEKKLEDYYK IDEKGLIKGK TQSQLLIIDR GFDPVSTVLH
260 270 280 290 300
ELTFQAMAYD LLPIENDTYK YKTDGKEKEA VLEEDDDLWV RVRHRHIAVV
310 320 330 340 350
LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ISKQVVHLNL
360 370 380 390 400
AEDCMNKFKL NIEKLCKTEQ DLALGTDAEG QRVKDSMLVL LPVLLNKNHD
410 420 430 440 450
NCDKIRAVLL YIFGINGTTE ENLDRLIHNV KIEDDSDMIR NWSHLGVPIV
460 470 480 490 500
PPSQQAKPLR KDRSAEETFQ LSRWTPFIKD IMEDAIDNRL DSKEWPYCSR
510 520 530 540 550
CPAVWNGSGA VSARQKPRTN YLELDRKNGS RLIIFVIGGI TYSEMRCAYE
560 570 580 590
VSQAHKSCEV IIGSTHILTP RKLLDDIKML NKSKDKVSFK DE
Length:592
Mass (Da):67,942
Last modified:November 1, 1996 - v1
Checksum:i7874B71DE107871A
GO
Isoform 2 (identifier: Q60770-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     344-370: Missing.
     484-511: Missing.

Note: No experimental confirmation available.
Show »
Length:537
Mass (Da):61,678
Checksum:i0DBB266AF1E7D54C
GO

Sequence cautioni

The sequence BAE41375 differs from that shown. Reason: Frameshift at position 512.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123K → R in BAE31079 (PubMed:16141072).Curated1
Sequence conflicti166N → Y in BAE23717 (PubMed:16141072).Curated1
Sequence conflicti303E → Q in BAE33068 (PubMed:16141072).Curated1
Sequence conflicti361N → S in BAE33068 (PubMed:16141072).Curated1
Sequence conflicti467E → G in BAE23717 (PubMed:16141072).Curated1
Sequence conflicti489R → K in BAE31079 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037061344 – 370Missing in isoform 2. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_037062484 – 511Missing in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19521 mRNA. Translation: AAA69913.1.
D30798 mRNA. Translation: BAA19478.1.
AK138609 mRNA. Translation: BAE23717.1.
AK151466 mRNA. Translation: BAE30424.1.
AK152261 mRNA. Translation: BAE31079.1.
AK155133 mRNA. Translation: BAE33068.1.
AK169799 mRNA. Translation: BAE41375.1. Frameshift.
AL671894, AL671917 Genomic DNA. Translation: CAM20601.1.
AL671917, AL671894 Genomic DNA. Translation: CAM17755.1.
BC062901 mRNA. Translation: AAH62901.1.
CCDSiCCDS17768.1. [Q60770-1]
PIRiI49239.
RefSeqiNP_035634.1. NM_011504.1. [Q60770-1]
UniGeneiMm.316894.

Genome annotation databases

EnsembliENSMUST00000102621; ENSMUSP00000099681; ENSMUSG00000027882. [Q60770-1]
GeneIDi20912.
KEGGimmu:20912.
UCSCiuc008qzr.3. mouse. [Q60770-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19521 mRNA. Translation: AAA69913.1.
D30798 mRNA. Translation: BAA19478.1.
AK138609 mRNA. Translation: BAE23717.1.
AK151466 mRNA. Translation: BAE30424.1.
AK152261 mRNA. Translation: BAE31079.1.
AK155133 mRNA. Translation: BAE33068.1.
AK169799 mRNA. Translation: BAE41375.1. Frameshift.
AL671894, AL671917 Genomic DNA. Translation: CAM20601.1.
AL671917, AL671894 Genomic DNA. Translation: CAM17755.1.
BC062901 mRNA. Translation: AAH62901.1.
CCDSiCCDS17768.1. [Q60770-1]
PIRiI49239.
RefSeqiNP_035634.1. NM_011504.1. [Q60770-1]
UniGeneiMm.316894.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PUKX-ray3.05A/B1-592[»]
ProteinModelPortaliQ60770.
SMRiQ60770.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203566. 1 interactor.
DIPiDIP-60305N.
IntActiQ60770. 7 interactors.
MINTiMINT-4136068.
STRINGi10090.ENSMUSP00000099681.

PTM databases

iPTMnetiQ60770.
PhosphoSitePlusiQ60770.
SwissPalmiQ60770.

Proteomic databases

EPDiQ60770.
PaxDbiQ60770.
PeptideAtlasiQ60770.
PRIDEiQ60770.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102621; ENSMUSP00000099681; ENSMUSG00000027882. [Q60770-1]
GeneIDi20912.
KEGGimmu:20912.
UCSCiuc008qzr.3. mouse. [Q60770-1]

Organism-specific databases

CTDi6814.
MGIiMGI:107362. Stxbp3.

Phylogenomic databases

eggNOGiKOG1300. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00390000005206.
HOGENOMiHOG000232146.
HOVERGENiHBG052710.
InParanoidiQ60770.
KOiK15301.
OMAiRKDRSTE.
OrthoDBiEOG091G0WUI.
PhylomeDBiQ60770.
TreeFamiTF313242.

Enzyme and pathway databases

ReactomeiR-MMU-114516. Disinhibition of SNARE formation.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

EvolutionaryTraceiQ60770.
PROiQ60770.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027882.
CleanExiMM_STXBP3A.
ExpressionAtlasiQ60770. baseline and differential.
GenevisibleiQ60770. MM.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTXB3_MOUSE
AccessioniPrimary (citable) accession number: Q60770
Secondary accession number(s): Q3TE73
, Q3U2S2, Q3U8D9, Q3UA91, Q3UUB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.