Reviewed, UniProtKB/Swiss-Prot Q60760 (GRB10_MOUSE)

Names and origin

Protein names

Recommended name:
    Growth factor receptor-bound protein 10
Alternative name(s):
    GRB10 adapter protein

Gene names

Name:

Grb10

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a functional role in insulin and IGF-I signaling. May serve to positively link the insulin and IGF-I receptors to an uncharacterized mitogenic signaling pathway. Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds activated platelet-derived growth factor receptor and epidermal growth factor receptor.
Subunit structure	Interacts with GIGYF1/PERQ1 and GIGYF2/TNRC15. Ref.6
Sequence similarities	Belongs to the GRB7/10/14 family. Contains 1 PH domain. Contains 1 Ras-associating domain. Contains 1 SH2 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	SH2 domain
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	insulin-like growth factor receptor signaling pathway Ref.6 Inferred from physical interaction. Source: MGI negative regulation of glucose import Inferred from mutant phenotype. Source: UniProtKB negative regulation of insulin receptor signaling pathway Inferred from mutant phenotype. Source: UniProtKB
Cellular component	cytosol Traceable author statement. Source: MGI
Molecular function	SH3/SH2 adaptor activity Ref.2 Inferred from physical interaction. Source: MGI insulin receptor binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Nedd4	P46935	4	EBI-861810,EBI-773516

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q60760-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q60760-2)

The sequence of this isoform differs from the canonical sequence as follows:
     117-141: Missing.

Isoform 3 (identifier: Q60760-3)

The sequence of this isoform differs from the canonical sequence as follows:
     117-196: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Growth factor receptor-bound protein 10		PRO_0000150347
Regions
	Domain	194 – 278	85	Ras-associating
	Domain	318 – 427	110	PH
	Domain	520 – 601	82	SH2
Amino acid modifications
	Modified residue	50	1	Phosphoserine By similarity
	Modified residue	96	1	Phosphoserine Ref.7
	Modified residue	432	1	Phosphotyrosine By similarity
	Modified residue	458	1	Phosphoserine Ref.8
Natural variations
	Alternative sequence	117 – 196	80	Missing in isoform 3.		VSP_012379
	Alternative sequence	117 – 141	25	Missing in isoform 2.		VSP_001844
Experimental info
	Sequence conflict	491 – 492	2	KR → NG in AAB53687. Ref.1
	Sequence conflict	555	1	A → T in BAC28088. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 6FC737E8F35468BB	FASTA	621	70,585
	10 20 30 40 50 60 MNNDINSSVE SLNSACNMQS DTDTAPLLED GQHASNQGAA SSSRGQPQAS PRQKMQRSQP 70 80 90 100 110 120 VHILRRLQEE DQQLRTASLP AIPNPFPELT GAAPGSPPSV APSSLPPPPS QPPAKHCGRC 130 140 150 160 170 180 EKWIPGENTR GNGKRKIWRW QFPPGFQLSK LTRPGLWTKT TARFSKKQPK NQCPTDTVNP 190 200 210 220 230 240 VARMPTSQME KLRLRKDVKV FSEDGTSKVV EILTDMTARD LCQLLVYKSH CVDDNSWTLV 250 260 270 280 290 300 EHHPQLGLER CLEDHEIVVQ VESTMPSESK FLFRKNYAKY EFFKNPVNFF PDQMVNWCQQ 310 320 330 340 350 360 SNGGQAQLLQ NFLNTSSCPE IQGFLQVKEV GRKSWKKLYV CLRRSGLYYS TKGTSKEPRH 370 380 390 400 410 420 LQLLADLEES SIFYLIAGKK QYNAPNEHGM CIKPNKAKTE MKELRLLCAE DEQIRTCWMT 430 440 450 460 470 480 AFRLLKYGML LYQNYRIPQR KGLPPPFNAP MRSVSENSLV AMDFSGQIGR VIDNPAEAQS 490 500 510 520 530 540 AALEEGHAWR KRSTRMNILS SQSPLHPSTL NAVIHRTQHW FHGRISREES HRIIKQQGLV 550 560 570 580 590 600 DGLFLLRDSQ SNPKAFVLTL CHHQKIKNFQ ILPCEDDGQT FFTLDDGNTK FSDLIQLVDF 610 620 YQLNKGVLPC KLKHHCIRVA L
	Isoform 2. Checksum: EB13CA896DF41533	FASTA	596	67,543
	10 20 30 40 50 60 MNNDINSSVE SLNSACNMQS DTDTAPLLED GQHASNQGAA SSSRGQPQAS PRQKMQRSQP 70 80 90 100 110 120 VHILRRLQEE DQQLRTASLP AIPNPFPELT GAAPGSPPSV APSSLPPPPS QPPAKHFPPG 130 140 150 160 170 180 FQLSKLTRPG LWTKTTARFS KKQPKNQCPT DTVNPVARMP TSQMEKLRLR KDVKVFSEDG 190 200 210 220 230 240 TSKVVEILTD MTARDLCQLL VYKSHCVDDN SWTLVEHHPQ LGLERCLEDH EIVVQVESTM 250 260 270 280 290 300 PSESKFLFRK NYAKYEFFKN PVNFFPDQMV NWCQQSNGGQ AQLLQNFLNT SSCPEIQGFL 310 320 330 340 350 360 QVKEVGRKSW KKLYVCLRRS GLYYSTKGTS KEPRHLQLLA DLEESSIFYL IAGKKQYNAP 370 380 390 400 410 420 NEHGMCIKPN KAKTEMKELR LLCAEDEQIR TCWMTAFRLL KYGMLLYQNY RIPQRKGLPP 430 440 450 460 470 480 PFNAPMRSVS ENSLVAMDFS GQIGRVIDNP AEAQSAALEE GHAWRKRSTR MNILSSQSPL 490 500 510 520 530 540 HPSTLNAVIH RTQHWFHGRI SREESHRIIK QQGLVDGLFL LRDSQSNPKA FVLTLCHHQK 550 560 570 580 590 IKNFQILPCE DDGQTFFTLD DGNTKFSDLI QLVDFYQLNK GVLPCKLKHH CIRVAL
	Isoform 3. Checksum: A8FA9ED57C85F674	FASTA	541	61,218
	10 20 30 40 50 60 MNNDINSSVE SLNSACNMQS DTDTAPLLED GQHASNQGAA SSSRGQPQAS PRQKMQRSQP 70 80 90 100 110 120 VHILRRLQEE DQQLRTASLP AIPNPFPELT GAAPGSPPSV APSSLPPPPS QPPAKHDVKV 130 140 150 160 170 180 FSEDGTSKVV EILTDMTARD LCQLLVYKSH CVDDNSWTLV EHHPQLGLER CLEDHEIVVQ 190 200 210 220 230 240 VESTMPSESK FLFRKNYAKY EFFKNPVNFF PDQMVNWCQQ SNGGQAQLLQ NFLNTSSCPE 250 260 270 280 290 300 IQGFLQVKEV GRKSWKKLYV CLRRSGLYYS TKGTSKEPRH LQLLADLEES SIFYLIAGKK 310 320 330 340 350 360 QYNAPNEHGM CIKPNKAKTE MKELRLLCAE DEQIRTCWMT AFRLLKYGML LYQNYRIPQR 370 380 390 400 410 420 KGLPPPFNAP MRSVSENSLV AMDFSGQIGR VIDNPAEAQS AALEEGHAWR KRSTRMNILS 430 440 450 460 470 480 SQSPLHPSTL NAVIHRTQHW FHGRISREES HRIIKQQGLV DGLFLLRDSQ SNPKAFVLTL 490 500 510 520 530 540 CHHQKIKNFQ ILPCEDDGQT FFTLDDGNTK FSDLIQLVDF YQLNKGVLPC KLKHHCIRVA L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cloning of Grb10 reveals a new family of SH2 domain proteins." Ooi J., Yajnik V., Immanuel D., Gordon M., Moskow J.J., Buchberg A., Margolis B. Oncogene 10:1621-1630(1995) [PubMed: 7731717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1). Strain: Swiss.
[2]	"The adapter protein Grb10 associates preferentially with the insulin receptor as compared with the IGF-I receptor in mouse fibroblasts." Laviola L., Giorgino F., Chow J.C., Baquero J.A., Hansen H., Ooi J., Zhu J., Riedel H., Smith R.J. J. Clin. Invest. 99:830-837(1997) [PubMed: 9062339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J.
[4]	Griffiths C., Sycamore N. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Eye and Olfactory epithelium.
[6]	"Two novel proteins that are linked to insulin-like growth factor (IGF-I) receptors by the Grb10 adapter and modulate IGF-I signaling." Giovannone B., Lee E., Laviola L., Giorgino F., Cleveland K.A., Smith R.J. J. Biol. Chem. 278:31564-31573(2003) [PubMed: 12771153] [Abstract] Cited for: INTERACTION WITH GIGYF1 AND GIGYF2.
[7]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, MASS SPECTROMETRY. Tissue: Brain.
[8]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18996 Genomic DNA. Translation: AAB53687.1. AF022072 mRNA. Translation: AAB72103.1. AK030727 mRNA. Translation: BAC27100.1. AK032927 mRNA. Translation: BAC28088.1. AL645803, AL663087 Genomic DNA. Translation: CAI23990.1. AL645803, AL663087 Genomic DNA. Translation: CAI23991.1. AL663087, AL645803 Genomic DNA. Translation: CAI25685.1. AL663087, AL645803 Genomic DNA. Translation: CAI25687.1. BC016111 mRNA. Translation: AAH16111.1. BC053842 mRNA. Translation: AAH53842.1. Different initiation.
IPI	IPI00127976. IPI00331684. IPI00473353.
PIR	I49199.
UniGene	Mm.273117
3D structure databases
HSSP	HSSP built from PDB template 1NRV based on UniProtKB Q13322.
SMR	Q60760. Positions 514-618.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:446N.
IntAct	Q60760. 1 interaction.
PTM databases
PhosphoSite	Q60760.
Genome annotation databases
Ensembl	ENSMUSG00000020176. Mus musculus. [Contig view]
NMPDR	fig|10090.3.peg.23293.
Organism-specific databases
MGI	MGI:103232. Grb10.
Phylogenomic databases
HOGENOM	Q60760.
HOVERGEN	Q60760.
OMA	Q60760. HIVKVWS.
Gene expression databases
ArrayExpress	Q60760.
Bgee	Q60760.
CleanEx	MM_GRB10.
GermOnline	ENSMUSG00000020176. Mus musculus.
Family and domain databases
InterPro	IPR015042. BPS. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000159. Ras-assoc. IPR000980. SH2. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF08947. BPS. 1 hit. PF00169. PH. 1 hit. PF00788. RA. 1 hit. PF00017. SH2. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN.
ProDom	PD000093. SH2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00233. PH. 1 hit. SM00314. RA. 1 hit. SM00252. SH2. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50200. RA. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

GRB10_MOUSE

Accession

Primary (citable) accession number: Q60760
Secondary accession number(s): O35352 , Q7TSA4, Q8BSH4, Q8BSS5, Q91WC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)