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Protein

Transcription factor 15

Gene

Tcf15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as an early transcriptional regulator, involved in the patterning of the mesoderm and in lineage determination of cell types derived from the mesoderm.

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • ear development Source: MGI
  • eating behavior Source: MGI
  • establishment of epithelial cell apical/basal polarity Source: MGI
  • mesenchymal to epithelial transition Source: MGI
  • muscle organ development Source: MGI
  • muscle organ morphogenesis Source: MGI
  • neuromuscular process controlling posture Source: MGI
  • paraxial mesoderm development Source: MGI
  • post-anal tail morphogenesis Source: MGI
  • regulation of extracellular matrix organization Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • respiratory system process Source: MGI
  • skeletal system morphogenesis Source: MGI
  • skin development Source: MGI
  • somitogenesis Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 15
Short name:
TCF-15
Alternative name(s):
Meso1
Paraxis
Protein bHLH-EC2
Gene namesi
Name:Tcf15
Synonyms:Bhlhec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:104664. Tcf15.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Transcription factor 15PRO_0000127462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60756.
PRIDEiQ60756.

PTM databases

PhosphoSiteiQ60756.

Expressioni

Tissue specificityi

Expressed in heart and skeletal muscle.

Developmental stagei

At 7.5 dpc, low expression is detected in a subdomain of the primitive mesoderm. At 8.5 dpc, expressed at high levels throughout the uncompartmentalized epithelial somites and in the rostral paraxial mesoderm. By 9.5 dpc, expression is confined to the somite and is most prominent in the myotome and dermatome. At 10 dpc, expression in somite declines in the myotome but persists at high level in the dermatome. At 10.5 dpc, expression is seen only in the somites in the caudal portion of the embryo.

Gene expression databases

BgeeiQ60756.
CleanExiMM_TCF15.
GenevisibleiQ60756. MM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein.By similarity

Protein-protein interaction databases

BioGridi204001. 2 interactions.
STRINGi10090.ENSMUSP00000086511.

Structurei

3D structure databases

ProteinModelPortaliQ60756.
SMRiQ60756. Positions 64-126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 12253bHLHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi50 – 6415Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00760000119097.
HOGENOMiHOG000231706.
HOVERGENiHBG036149.
InParanoidiQ60756.
KOiK09070.
OMAiNQRKGGS.
OrthoDBiEOG7G1V82.
PhylomeDBiQ60756.
TreeFamiTF315153.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFALLRPVG AHVLYPDVRL LSEDEENRSE SDASDQSFGC CEGLEAARRG
60 70 80 90 100
PGPGSGRRAS NGAGPVVVVR QRQAANARER DRTQSVNTAF TALRTLIPTE
110 120 130 140 150
PVDRKLSKIE TLRLASSYIA HLANVLLLGD AADDGQPCFR AAGGGKSAVP
160 170 180 190
AADGRQPRSI CTFCLSNQRK GGSRRDLGGS CLKVRGVAPL RGPRR
Length:195
Mass (Da):20,721
Last modified:November 25, 2002 - v2
Checksum:iF659B94C5F125E56
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti130 – 1312DA → ELT in AAA86825 (PubMed:7729571).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18658 mRNA. Translation: AAA86825.1.
BC027533 mRNA. Translation: AAH27533.1.
U20164 Genomic DNA. Translation: AAB48215.1.
CCDSiCCDS16878.1.
RefSeqiNP_033354.2. NM_009328.2.
UniGeneiMm.3881.

Genome annotation databases

EnsembliENSMUST00000089112; ENSMUSP00000086511; ENSMUSG00000068079.
GeneIDi21407.
KEGGimmu:21407.
UCSCiuc008nex.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18658 mRNA. Translation: AAA86825.1.
BC027533 mRNA. Translation: AAH27533.1.
U20164 Genomic DNA. Translation: AAB48215.1.
CCDSiCCDS16878.1.
RefSeqiNP_033354.2. NM_009328.2.
UniGeneiMm.3881.

3D structure databases

ProteinModelPortaliQ60756.
SMRiQ60756. Positions 64-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204001. 2 interactions.
STRINGi10090.ENSMUSP00000086511.

PTM databases

PhosphoSiteiQ60756.

Proteomic databases

PaxDbiQ60756.
PRIDEiQ60756.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089112; ENSMUSP00000086511; ENSMUSG00000068079.
GeneIDi21407.
KEGGimmu:21407.
UCSCiuc008nex.1. mouse.

Organism-specific databases

CTDi6939.
MGIiMGI:104664. Tcf15.

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00760000119097.
HOGENOMiHOG000231706.
HOVERGENiHBG036149.
InParanoidiQ60756.
KOiK09070.
OMAiNQRKGGS.
OrthoDBiEOG7G1V82.
PhylomeDBiQ60756.
TreeFamiTF315153.

Miscellaneous databases

NextBioi300680.
PROiQ60756.
SOURCEiSearch...

Gene expression databases

BgeeiQ60756.
CleanExiMM_TCF15.
GenevisibleiQ60756. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Paraxis: a basic helix-loop-helix protein expressed in paraxial mesoderm and developing somites."
    Burgess R., Cserjesi P., Ligon K.L., Olson E.N.
    Dev. Biol. 168:296-306(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NIH Swiss.
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "Genomic organization and chromosomal localization of the gene TCF15 encoding the early mesodermal basic helix-loop-helix factor bHLH-EC2."
    Hidai H., Quertermous E.E., Espinosa R., Le Beau M.M., Quertermous T.
    Genomics 30:598-601(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
    Strain: 129/SvJ.
  4. "Meso1, a basic-helix-loop-helix protein involved in mammalian presomitic mesoderm development."
    Blanar M.A., Crossley P.H., Peters K.G., Steingrimsson E., Copeland N.G., Jenkins N.A., Martin G.R., Rutter W.J.
    Proc. Natl. Acad. Sci. U.S.A. 92:5870-5874(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Cloning and characterization of a basic helix-loop-helix protein expressed in early mesoderm and the developing somites."
    Quertermous E.E., Hidai H., Blanar M.A., Quertermous T.
    Proc. Natl. Acad. Sci. U.S.A. 91:7066-7070(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue.

Entry informationi

Entry nameiTCF15_MOUSE
AccessioniPrimary (citable) accession number: Q60756
Secondary accession number(s): Q60788
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: February 17, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.