Q60756 (TCF15_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor 15
Short name=TCF-15

Alternative name(s):
Meso1
Paraxis
Protein bHLH-EC2

Gene names

Name:	Tcf15
Synonyms:	Bhlhec2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as an early transcriptional regulator, involved in the patterning of the mesoderm and in lineage determination of cell types derived from the mesoderm.
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed in heart and skeletal muscle.
Developmental stage	At 7.5 dpc, low expression is detected in a subdomain of the primitive mesoderm. At 8.5 dpc, expressed at high levels throughout the uncompartmentalized epithelial somites and in the rostral paraxial mesoderm. By 9.5 dpc, expression is confined to the somite and is most prominent in the myotome and dermatome. At 10 dpc, expression in somite declines in the myotome but persists at high level in the dermatome. At 10.5 dpc, expression is seen only in the somites in the caudal portion of the embryo.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	death Inferred from mutant phenotype. Source: MGI ear development Inferred from mutant phenotype. Source: MGI eating behavior Inferred from mutant phenotype. Source: MGI muscle organ morphogenesis Inferred from mutant phenotype. Source: MGI neuromuscular process controlling posture Inferred from mutant phenotype. Source: MGI paraxial mesoderm development Inferred from mutant phenotype. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW respiratory system process Inferred from mutant phenotype. Source: MGI skeletal system morphogenesis Inferred from mutant phenotype. Source: MGI skin development Inferred from mutant phenotype. Source: MGI somitogenesis Inferred from mutant phenotype. Source: MGI tail morphogenesis Inferred from mutant phenotype. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Transcription factor 15		PRO_0000127462
Regions
	Domain	83 – 123	41	Helix-loop-helix motif
	DNA binding	70 – 82	13	Basic motif
	Compositional bias	50 – 64	15	Gly-rich
Experimental info
	Sequence conflict	130 – 131	2	DA → ELT in AAA86825. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q60756 [UniParc]. Last modified November 25, 2002. Version 2. Checksum: F659B94C5F125E56	FASTA	195	20,721
	10 20 30 40 50 60 MAFALLRPVG AHVLYPDVRL LSEDEENRSE SDASDQSFGC CEGLEAARRG PGPGSGRRAS 70 80 90 100 110 120 NGAGPVVVVR QRQAANARER DRTQSVNTAF TALRTLIPTE PVDRKLSKIE TLRLASSYIA 130 140 150 160 170 180 HLANVLLLGD AADDGQPCFR AAGGGKSAVP AADGRQPRSI CTFCLSNQRK GGSRRDLGGS 190 CLKVRGVAPL RGPRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Paraxis: a basic helix-loop-helix protein expressed in paraxial mesoderm and developing somites." Burgess R., Cserjesi P., Ligon K.L., Olson E.N. Dev. Biol. 168:296-306(1995) [PubMed: 7729571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NIH Swiss. Tissue: Embryo.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"Genomic organization and chromosomal localization of the gene TCF15 encoding the early mesodermal basic helix-loop-helix factor bHLH-EC2." Hidai H., Quertermous E.E., Espinosa R., Le Beau M.M., Quertermous T. Genomics 30:598-601(1995) [PubMed: 8825648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35. Strain: 129/SvJ.
[4]	"Meso1, a basic-helix-loop-helix protein involved in mammalian presomitic mesoderm development." Blanar M.A., Crossley P.H., Peters K.G., Steingrimsson E., Copeland N.G., Jenkins N.A., Martin G.R., Rutter W.J. Proc. Natl. Acad. Sci. U.S.A. 92:5870-5874(1995) [PubMed: 7597044] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Cloning and characterization of a basic helix-loop-helix protein expressed in early mesoderm and the developing somites." Quertermous E.E., Hidai H., Blanar M.A., Quertermous T. Proc. Natl. Acad. Sci. U.S.A. 91:7066-7070(1994) [PubMed: 8041747] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18658 mRNA. Translation: AAA86825.1. BC027533 mRNA. Translation: AAH27533.1. U20164 Genomic DNA. Translation: AAB48215.1.
IPI	IPI00222980.
RefSeq	NP_033354.2. NM_009328.2.
UniGene	Mm.3881.
3D structure databases
ProteinModelPortal	Q60756.
SMR	Q60756. Positions 71-127.
ModBase	Search...
Protein-protein interaction databases
STRING	Q60756.
PTM databases
PhosphoSite	Q60756.
Proteomic databases
PRIDE	Q60756.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000089112; ENSMUSP00000086511; ENSMUSG00000068079.
GeneID	21407.
KEGG	mmu:21407.
Organism-specific databases
CTD	6939.
MGI	MGI:104664. Tcf15.
Phylogenomic databases
GeneTree	ENSGT00590000082747.
HOGENOM	HBG715362.
HOVERGEN	HBG036149.
InParanoid	Q60756.
OMA	NQRKGGS.
OrthoDB	EOG4ZPDWJ.
PhylomeDB	Q60756.
Gene expression databases
ArrayExpress	Q60756.
Bgee	Q60756.
CleanEx	MM_TCF15.
Genevestigator	Q60756.
GermOnline	ENSMUSG00000068079. Mus musculus.
Family and domain databases
InterPro	IPR011598. HLH_DNA-bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
KO	K09070.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	300680.
SOURCE	Search...

Entry information

Entry name

TCF15_MOUSE

Accession

Primary (citable) accession number: Q60756
Secondary accession number(s): Q60788

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	November 25, 2002
Last modified:	November 16, 2011
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program