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Q60739

- BAG1_MOUSE

UniProt

Q60739 - BAG1_MOUSE

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Protein

BAG family molecular chaperone regulator 1

Gene

Bag1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.1 Publication

GO - Molecular functioni

  1. phosphoprotein binding Source: MGI

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. negative regulation of apoptotic process Source: MGI
  3. negative regulation of motor neuron apoptotic process Source: MGI
  4. negative regulation of protein phosphorylation Source: MGI
  5. neuron differentiation Source: MGI
  6. positive regulation of neuron apoptotic process Source: MGI
  7. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  8. protein localization to mitochondrion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
BAG family molecular chaperone regulator 1
Short name:
BAG-1
Alternative name(s):
Bcl-2-associated athanogene 1
Gene namesi
Name:Bag1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:108047. Bag1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: MGI
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355BAG family molecular chaperone regulator 1PRO_0000002782Add
BLAST

Post-translational modificationi

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ60739.
PaxDbiQ60739.
PRIDEiQ60739.

PTM databases

PhosphoSiteiQ60739.

Expressioni

Tissue specificityi

Isoform 2 is expressed in the heart, lung, kidney and spinal cord. Isoform 1 and isoform 2 are expressed in hematopoietic cell lines. The levels of isoform 2 are relatively constant in all the cell lines examined while the levels of isoform 1 are more variable (at protein level). Isoform 1 is expressed in the lung and kidney. Isoform 2 is expressed in various tissues, with highest levels in testis and stomach.2 Publications

Gene expression databases

BgeeiQ60739.
CleanExiMM_BAG1.
ExpressionAtlasiQ60739. baseline and differential.
GenevestigatoriQ60739.

Interactioni

Subunit structurei

Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner (By similarity).By similarity

Protein-protein interaction databases

BioGridi198298. 6 interactions.
DIPiDIP-272N.

Structurei

Secondary structure

1
355
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi149 – 1535
Beta strandi155 – 1606
Beta strandi163 – 1686
Beta strandi171 – 1733
Beta strandi175 – 1773
Helixi180 – 19112
Helixi195 – 1973
Beta strandi200 – 2023
Beta strandi205 – 2106
Turni215 – 2184
Beta strandi220 – 2223
Beta strandi226 – 2283
Helixi234 – 26835
Beta strandi270 – 2723
Helixi274 – 30330
Helixi312 – 34635

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I6ZNMR-A226-355[»]
2LWPNMR-A137-233[»]
2M8SNMR-A137-233[»]
ProteinModelPortaliQ60739.
SMRiQ60739. Positions 137-355.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60739.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati103 – 10861
Repeati111 – 11662
Repeati117 – 12263
Repeati123 – 12864
Repeati129 – 13465
Repeati141 – 14666
Repeati147 – 15267
Domaini154 – 23481Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST
Domaini256 – 33681BAGPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni111 – 209997 X 6 AA tandem repeat of E-E-X(4)Add
BLAST
Regioni182 – 22948Interaction with HSPA8By similarityAdd
BLAST
Regioni226 – 355130Interaction with PPP1R15ABy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 BAG domain.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG72809.
GeneTreeiENSGT00450000040296.
HOGENOMiHOG000286009.
HOVERGENiHBG000236.
InParanoidiQ60739.
KOiK09555.
OrthoDBiEOG7J4482.
PhylomeDBiQ60739.

Family and domain databases

Gene3Di1.20.58.120. 1 hit.
InterProiIPR003103. BAG_domain.
IPR017093. Molecular_chp_reg_BAG_1.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02179. BAG. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
PIRSFiPIRSF037029. BAG_1. 1 hit.
SMARTiSM00264. BAG. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS51035. BAG. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: Q60739-1) [UniParc]FASTAAdd to Basket

Also known as: BAG-1L, p50

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGRSAARRP RGDREPLGPR LRAPRPAREP RQSESRAERG LPPSQRSSVR
60 70 80 90 100
SAASGHDRST RGAPAGACKP RVKKKVRPRS SQSEKVGSSS RELTRSKKVT
110 120 130 140 150
RSKNVTGTQV EEVTKIEEAT QTEEVTVAEE VTQTDNMAKT EEMVQTEEME
160 170 180 190 200
TPRLSVIVTH SNERYDLLVT PQQGNSEPVV QDLAQLVEEA TGVPLPFQKL
210 220 230 240 250
IFKGKSLKEM ETPLSALGMQ NGCRVMLIGE KSNPEEEVEL KKLKDLEVSA
260 270 280 290 300
EKIANHLQEL NKELSGIQQG FLAKELQAEA LCKLDRKVKA TIEQFMKILE
310 320 330 340 350
EIDTMVLPEQ FKDSRLKRKN LVKKVQVFLA ECDTVEQYIC QETERLQSTN

LALAE
Length:355
Mass (Da):39,740
Last modified:July 15, 1999 - v3
Checksum:i077A765CA869D3A7
GO
Isoform 2 (identifier: Q60739-2) [UniParc]FASTAAdd to Basket

Also known as: BAG-1S, p32

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.

Show »
Length:219
Mass (Da):24,875
Checksum:i569C8AFFFBB6FBD1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641P → A in AAH93509. (PubMed:15489334)Curated
Sequence conflicti64 – 641P → A in AAH69918. (PubMed:15489334)Curated
Sequence conflicti64 – 641P → A in AAH03722. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 136136Missing in isoform 2. 1 PublicationVSP_018667Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF022223 mRNA. Translation: AAC34259.1.
AK009149 mRNA. Translation: BAB26106.1.
AL837521 Genomic DNA. Translation: CAM19843.1.
CH466538 Genomic DNA. Translation: EDL05422.1.
BC003722 mRNA. Translation: AAH03722.2.
BC069918 mRNA. Translation: AAH69918.2.
BC093509 mRNA. Translation: AAH93509.2.
CCDSiCCDS38713.1. [Q60739-1]
CCDS51139.1. [Q60739-2]
RefSeqiNP_001165210.1. NM_001171739.1. [Q60739-2]
NP_033866.4. NM_009736.3.
UniGeneiMm.688.

Genome annotation databases

EnsembliENSMUST00000108089; ENSMUSP00000103724; ENSMUSG00000028416. [Q60739-2]
ENSMUST00000191273; ENSMUSP00000139864; ENSMUSG00000028416. [Q60739-2]
GeneIDi12017.
KEGGimmu:12017.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF022223 mRNA. Translation: AAC34259.1 .
AK009149 mRNA. Translation: BAB26106.1 .
AL837521 Genomic DNA. Translation: CAM19843.1 .
CH466538 Genomic DNA. Translation: EDL05422.1 .
BC003722 mRNA. Translation: AAH03722.2 .
BC069918 mRNA. Translation: AAH69918.2 .
BC093509 mRNA. Translation: AAH93509.2 .
CCDSi CCDS38713.1. [Q60739-1 ]
CCDS51139.1. [Q60739-2 ]
RefSeqi NP_001165210.1. NM_001171739.1. [Q60739-2 ]
NP_033866.4. NM_009736.3.
UniGenei Mm.688.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1I6Z NMR - A 226-355 [» ]
2LWP NMR - A 137-233 [» ]
2M8S NMR - A 137-233 [» ]
ProteinModelPortali Q60739.
SMRi Q60739. Positions 137-355.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198298. 6 interactions.
DIPi DIP-272N.

PTM databases

PhosphoSitei Q60739.

Proteomic databases

MaxQBi Q60739.
PaxDbi Q60739.
PRIDEi Q60739.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000108089 ; ENSMUSP00000103724 ; ENSMUSG00000028416 . [Q60739-2 ]
ENSMUST00000191273 ; ENSMUSP00000139864 ; ENSMUSG00000028416 . [Q60739-2 ]
GeneIDi 12017.
KEGGi mmu:12017.

Organism-specific databases

CTDi 573.
MGIi MGI:108047. Bag1.

Phylogenomic databases

eggNOGi NOG72809.
GeneTreei ENSGT00450000040296.
HOGENOMi HOG000286009.
HOVERGENi HBG000236.
InParanoidi Q60739.
KOi K09555.
OrthoDBi EOG7J4482.
PhylomeDBi Q60739.

Miscellaneous databases

EvolutionaryTracei Q60739.
NextBioi 280235.
PROi Q60739.
SOURCEi Search...

Gene expression databases

Bgeei Q60739.
CleanExi MM_BAG1.
ExpressionAtlasi Q60739. baseline and differential.
Genevestigatori Q60739.

Family and domain databases

Gene3Di 1.20.58.120. 1 hit.
InterProi IPR003103. BAG_domain.
IPR017093. Molecular_chp_reg_BAG_1.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF02179. BAG. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view ]
PIRSFi PIRSF037029. BAG_1. 1 hit.
SMARTi SM00264. BAG. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS51035. BAG. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity."
    Takayama S., Sato T., Krajewski S., Kochel K., Irie S., Millan J.A., Reed J.C.
    Cell 80:279-284(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Tongue.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II and NMRI.
    Tissue: Mammary tumor.
  6. "Mammalian cells express two differently localized Bag-1 isoforms generated by alternative translation initiation."
    Packham G., Brimmell M., Cleveland J.L.
    Biochem. J. 328:807-813(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF ISOFORMS 1 AND 2, ALTERNATIVE INITIATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "Expression and location of Hsp70/Hsc-binding anti-apoptotic protein BAG-1 and its variants in normal tissues and tumor cell lines."
    Takayama S., Krajewski S., Krajewska M., Kitada S., Zapata J.M., Kochel K., Knee D., Scudiero D., Tudor G., Miller G.J., Miyashita T., Yamada M., Reed J.C.
    Cancer Res. 58:3116-3131(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF ISOFORMS 1 AND 2, ALTERNATIVE INITIATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators."
    Takayama S., Xie Z., Reed J.C.
    J. Biol. Chem. 274:781-786(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Alteration of the stability of Bag-1 protein in the control of olfactory neuronal apoptosis."
    Sourisseau T., Desbois C., Debure L., Bowtell D.D.L., Cato A.C.B., Schneikert J., Moyse E., Michel D.
    J. Cell Sci. 114:1409-1416(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SIAH2, UBIQUITINATION.

Entry informationi

Entry nameiBAG1_MOUSE
AccessioniPrimary (citable) accession number: Q60739
Secondary accession number(s): Q561N1, Q6IS45, Q9D7K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3