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Protein

BAG family molecular chaperone regulator 1

Gene

Bag1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.1 Publication

GO - Molecular functioni

  • phosphoprotein binding Source: MGI

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • chaperone cofactor-dependent protein refolding Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of motor neuron apoptotic process Source: MGI
  • negative regulation of protein phosphorylation Source: MGI
  • neuron differentiation Source: MGI
  • positive regulation of neuron apoptotic process Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein localization to mitochondrion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-MMU-3371453. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
BAG family molecular chaperone regulator 1
Short name:
BAG-1
Alternative name(s):
Bcl-2-associated athanogene 1
Gene namesi
Name:Bag1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:108047. Bag1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000027821 – 355BAG family molecular chaperone regulator 1Add BLAST355

Post-translational modificationi

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ60739.
PRIDEiQ60739.

PTM databases

iPTMnetiQ60739.
PhosphoSitePlusiQ60739.

Expressioni

Tissue specificityi

Isoform 2 is expressed in the heart, lung, kidney and spinal cord. Isoform 1 and isoform 2 are expressed in hematopoietic cell lines. The levels of isoform 2 are relatively constant in all the cell lines examined while the levels of isoform 1 are more variable (at protein level). Isoform 1 is expressed in the lung and kidney. Isoform 2 is expressed in various tissues, with highest levels in testis and stomach.2 Publications

Gene expression databases

BgeeiENSMUSG00000028416.
CleanExiMM_BAG1.
ExpressionAtlasiQ60739. baseline and differential.
GenevisibleiQ60739. MM.

Interactioni

Subunit structurei

Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner (By similarity).By similarity

GO - Molecular functioni

  • phosphoprotein binding Source: MGI

Protein-protein interaction databases

BioGridi198298. 6 interactors.
DIPiDIP-272N.
STRINGi10090.ENSMUSP00000030125.

Structurei

Secondary structure

1355
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi149 – 153Combined sources5
Beta strandi155 – 160Combined sources6
Beta strandi163 – 168Combined sources6
Beta strandi171 – 173Combined sources3
Beta strandi175 – 177Combined sources3
Helixi180 – 191Combined sources12
Helixi195 – 197Combined sources3
Beta strandi200 – 202Combined sources3
Beta strandi205 – 210Combined sources6
Turni215 – 218Combined sources4
Beta strandi220 – 222Combined sources3
Beta strandi226 – 228Combined sources3
Helixi234 – 268Combined sources35
Beta strandi270 – 272Combined sources3
Helixi274 – 303Combined sources30
Helixi312 – 346Combined sources35

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I6ZNMR-A226-355[»]
2LWPNMR-A137-233[»]
2M8SNMR-A137-233[»]
ProteinModelPortaliQ60739.
SMRiQ60739.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60739.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati103 – 10816
Repeati111 – 11626
Repeati117 – 12236
Repeati123 – 12846
Repeati129 – 13456
Repeati141 – 14666
Repeati147 – 15276
Domaini154 – 234Ubiquitin-likePROSITE-ProRule annotationAdd BLAST81
Domaini256 – 336BAGPROSITE-ProRule annotationAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni111 – 2097 X 6 AA tandem repeat of E-E-X(4)Add BLAST99
Regioni182 – 229Interaction with HSPA8By similarityAdd BLAST48
Regioni226 – 355Interaction with PPP1R15ABy similarityAdd BLAST130

Sequence similaritiesi

Contains 1 BAG domain.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IHYI. Eukaryota.
ENOG4111SUG. LUCA.
GeneTreeiENSGT00450000040296.
HOGENOMiHOG000286009.
HOVERGENiHBG000236.
InParanoidiQ60739.
KOiK09555.
PhylomeDBiQ60739.

Family and domain databases

Gene3Di1.20.58.120. 1 hit.
InterProiIPR003103. BAG_domain.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF02179. BAG. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00264. BAG. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF63491. SSF63491. 1 hit.
PROSITEiPS51035. BAG. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q60739-1) [UniParc]FASTAAdd to basket
Also known as: BAG-1L, p50

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGRSAARRP RGDREPLGPR LRAPRPAREP RQSESRAERG LPPSQRSSVR
60 70 80 90 100
SAASGHDRST RGAPAGACKP RVKKKVRPRS SQSEKVGSSS RELTRSKKVT
110 120 130 140 150
RSKNVTGTQV EEVTKIEEAT QTEEVTVAEE VTQTDNMAKT EEMVQTEEME
160 170 180 190 200
TPRLSVIVTH SNERYDLLVT PQQGNSEPVV QDLAQLVEEA TGVPLPFQKL
210 220 230 240 250
IFKGKSLKEM ETPLSALGMQ NGCRVMLIGE KSNPEEEVEL KKLKDLEVSA
260 270 280 290 300
EKIANHLQEL NKELSGIQQG FLAKELQAEA LCKLDRKVKA TIEQFMKILE
310 320 330 340 350
EIDTMVLPEQ FKDSRLKRKN LVKKVQVFLA ECDTVEQYIC QETERLQSTN

LALAE
Length:355
Mass (Da):39,740
Last modified:July 15, 1999 - v3
Checksum:i077A765CA869D3A7
GO
Isoform 2 (identifier: Q60739-2) [UniParc]FASTAAdd to basket
Also known as: BAG-1S, p32

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.

Show »
Length:219
Mass (Da):24,875
Checksum:i569C8AFFFBB6FBD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64P → A in AAH93509 (PubMed:15489334).Curated1
Sequence conflicti64P → A in AAH69918 (PubMed:15489334).Curated1
Sequence conflicti64P → A in AAH03722 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186671 – 136Missing in isoform 2. 1 PublicationAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022223 mRNA. Translation: AAC34259.1.
AK009149 mRNA. Translation: BAB26106.1.
AL837521 Genomic DNA. Translation: CAM19843.1.
CH466538 Genomic DNA. Translation: EDL05422.1.
BC003722 mRNA. Translation: AAH03722.2.
BC069918 mRNA. Translation: AAH69918.2.
BC093509 mRNA. Translation: AAH93509.2.
CCDSiCCDS38713.1. [Q60739-1]
CCDS51139.1. [Q60739-2]
RefSeqiNP_001165210.1. NM_001171739.1. [Q60739-2]
NP_033866.4. NM_009736.3.
UniGeneiMm.688.

Genome annotation databases

EnsembliENSMUST00000108089; ENSMUSP00000103724; ENSMUSG00000028416. [Q60739-2]
ENSMUST00000191273; ENSMUSP00000139864; ENSMUSG00000028416. [Q60739-2]
GeneIDi12017.
KEGGimmu:12017.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022223 mRNA. Translation: AAC34259.1.
AK009149 mRNA. Translation: BAB26106.1.
AL837521 Genomic DNA. Translation: CAM19843.1.
CH466538 Genomic DNA. Translation: EDL05422.1.
BC003722 mRNA. Translation: AAH03722.2.
BC069918 mRNA. Translation: AAH69918.2.
BC093509 mRNA. Translation: AAH93509.2.
CCDSiCCDS38713.1. [Q60739-1]
CCDS51139.1. [Q60739-2]
RefSeqiNP_001165210.1. NM_001171739.1. [Q60739-2]
NP_033866.4. NM_009736.3.
UniGeneiMm.688.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I6ZNMR-A226-355[»]
2LWPNMR-A137-233[»]
2M8SNMR-A137-233[»]
ProteinModelPortaliQ60739.
SMRiQ60739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198298. 6 interactors.
DIPiDIP-272N.
STRINGi10090.ENSMUSP00000030125.

PTM databases

iPTMnetiQ60739.
PhosphoSitePlusiQ60739.

Proteomic databases

PaxDbiQ60739.
PRIDEiQ60739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108089; ENSMUSP00000103724; ENSMUSG00000028416. [Q60739-2]
ENSMUST00000191273; ENSMUSP00000139864; ENSMUSG00000028416. [Q60739-2]
GeneIDi12017.
KEGGimmu:12017.

Organism-specific databases

CTDi573.
MGIiMGI:108047. Bag1.

Phylogenomic databases

eggNOGiENOG410IHYI. Eukaryota.
ENOG4111SUG. LUCA.
GeneTreeiENSGT00450000040296.
HOGENOMiHOG000286009.
HOVERGENiHBG000236.
InParanoidiQ60739.
KOiK09555.
PhylomeDBiQ60739.

Enzyme and pathway databases

ReactomeiR-MMU-3371453. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

EvolutionaryTraceiQ60739.
PROiQ60739.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028416.
CleanExiMM_BAG1.
ExpressionAtlasiQ60739. baseline and differential.
GenevisibleiQ60739. MM.

Family and domain databases

Gene3Di1.20.58.120. 1 hit.
InterProiIPR003103. BAG_domain.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF02179. BAG. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00264. BAG. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF63491. SSF63491. 1 hit.
PROSITEiPS51035. BAG. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAG1_MOUSE
AccessioniPrimary (citable) accession number: Q60739
Secondary accession number(s): Q561N1, Q6IS45, Q9D7K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1999
Last modified: November 2, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.