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Q60718

- ADAM2_MOUSE

UniProt

Q60718 - ADAM2_MOUSE

Protein

Disintegrin and metalloproteinase domain-containing protein 2

Gene

Adam2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (01 Feb 2003)
      Previous versions | rss
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    Functioni

    Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein By similarity.By similarity

    GO - Molecular functioni

    1. metalloendopeptidase activity Source: InterPro
    2. protein binding Source: MGI
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. adult behavior Source: MGI
    2. cell adhesion Source: MGI
    3. visual learning Source: MGI

    Keywords - Biological processi

    Cell adhesion

    Enzyme and pathway databases

    ReactomeiREACT_199072. Interaction With The Zona Pellucida.

    Protein family/group databases

    MEROPSiM12.950.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 2
    Short name:
    ADAM 2
    Alternative name(s):
    Fertilin subunit beta
    PH-30
    Short name:
    PH30
    PH30-beta
    Gene namesi
    Name:Adam2
    Synonyms:Ftnb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1340894. Adam2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 180162By similarityPRO_0000029046Add
    BLAST
    Chaini181 – 735555Disintegrin and metalloproteinase domain-containing protein 2PRO_0000029047Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi293 ↔ 376By similarity
    Disulfide bondi335 ↔ 360By similarity
    Disulfide bondi337 ↔ 342By similarity
    Glycosylationi359 – 3591N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi449 ↔ 469By similarity
    Glycosylationi463 – 4631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi569 – 5691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi585 – 5851N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi619 ↔ 630By similarity
    Disulfide bondi624 ↔ 636By similarity
    Disulfide bondi638 ↔ 647By similarity

    Post-translational modificationi

    The signal and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ60718.
    PRIDEiQ60718.

    PTM databases

    PhosphoSiteiQ60718.

    Expressioni

    Tissue specificityi

    Expressed specifically in testis.

    Gene expression databases

    BgeeiQ60718.
    CleanExiMM_ADAM2.
    GenevestigatoriQ60718.

    Interactioni

    Subunit structurei

    Heterodimer with ADAM1/fertilin subunit alpha.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60718.
    SMRiQ60718. Positions 185-610.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 686668ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini708 – 73528CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei687 – 70721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini184 – 381198Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini389 – 47688DisintegrinPROSITE-ProRule annotationAdd
    BLAST
    Domaini615 – 64834EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi480 – 611132Cys-richAdd
    BLAST

    Domaini

    A tripeptide motif (QDE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.By similarity

    Sequence similaritiesi

    Contains 1 disintegrin domain.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG242228.
    GeneTreeiENSGT00750000117474.
    HOGENOMiHOG000230883.
    HOVERGENiHBG103628.
    InParanoidiQ60718.
    KOiK06833.
    OMAiCGKLICK.
    OrthoDBiEOG7HB58N.
    PhylomeDBiQ60718.
    TreeFamiTF314733.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProiIPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view]
    PfamiPF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view]
    SMARTiSM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF57552. SSF57552. 1 hit.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q60718-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWLILLLLSG LSELGGLSQS QTEGTREKLH VQVTVPEKIR SVTSNGYETQ    50
    VTYNLKIEGK TYTLDLMQKP FLPPNFRVYS YDNAGIMRSL EQKFQNICYF 100
    QGYIEGYPNS MVIVSTCTGL RGFLQFGNVS YGIEPLESSS GFEHVIYQVE 150
    PEKGGALLYA EKDIDLRDSQ YKIRSIKPQR IVSHYLEIHI VVEKQMFEHI 200
    GADTAIVTQK IFQLIGLANA IFAPFNLTVI LSSLEFWMDE NKILTTGDAN 250
    KLLYRFLKWK QSYLVLRPHD MAFLLVYRNT TDYVGATYQG KMCDKNYAGG 300
    VALHPKAVTL ESLAIILVQL LSLSMGLAYD DVNKCQCGVP VCVMNPEAPH 350
    SSGVRAFSNC SMEDFSKFIT SQSSHCLQNQ PRLQPSYKMA VCGNGEVEED 400
    EICDCGKKGC AEMPPPCCNP DTCKLSDGSE CSSGICCNSC KLKRKGEVCR 450
    LAQDECDVTE YCNGTSEVCE DFFVQNGHPC DNRKWICING TCQSGEQQCQ 500
    DLFGIDAGFG SSECFWELNS KSDISGSCGI SAGGYKECPP NDRMCGKIIC 550
    KYQSENILKL RSATVIYANI SGHVCVSLEY PQGHNESQKM WVRDGTVCGS 600
    NKVCQNQKCV ADTFLGYDCN LEKCNHHGVC NNKKNCHCDP TYLPPDCKRM 650
    KDSYPGGSID SGNKERAEPI PVRPYIASAY RSKSPRWPFF LIIPFYVVIL 700
    VLIGMLVKVY SQRMKWRMDD FSSEEQFESE SESKD 735
    Length:735
    Mass (Da):82,375
    Last modified:February 1, 2003 - v2
    Checksum:i75EC8529CF5B8E2B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21W → R in AAD04207. 1 PublicationCurated
    Sequence conflicti17 – 204LSQS → IRHE in AAA74921. (PubMed:7750654)Curated
    Sequence conflicti24 – 252GT → A in AAA74921. (PubMed:7750654)Curated
    Sequence conflicti113 – 1131I → M in AAA90980. (PubMed:7593287)Curated
    Sequence conflicti234 – 2429LEFWMDENK → WNFGWMKQ in AAA74921. (PubMed:7750654)Curated
    Sequence conflicti246 – 2472TG → QA in AAA74921. (PubMed:7750654)Curated
    Sequence conflicti286 – 2861A → L in AAA74921. (PubMed:7750654)Curated
    Sequence conflicti331 – 3322DV → RRL in AAA74921. (PubMed:7750654)Curated
    Sequence conflicti382 – 3821R → T in AAD04207. 1 PublicationCurated
    Sequence conflicti658 – 6581S → T in AAA74921. (PubMed:7750654)Curated
    Sequence conflicti679 – 6791A → R in AAD04207. 1 PublicationCurated
    Sequence conflicti712 – 7121Q → P in AAA90980. (PubMed:7593287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16242 mRNA. Translation: AAA90980.1.
    U38806 mRNA. Translation: AAD04207.1.
    AK016550 mRNA. Translation: BAB30298.1.
    U22057 mRNA. Translation: AAA74921.1.
    CCDSiCCDS36959.1.
    RefSeqiNP_033748.2. NM_009618.2.
    UniGeneiMm.422850.

    Genome annotation databases

    EnsembliENSMUST00000022618; ENSMUSP00000022618; ENSMUSG00000022039.
    GeneIDi11495.
    KEGGimmu:11495.
    UCSCiuc007uju.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16242 mRNA. Translation: AAA90980.1 .
    U38806 mRNA. Translation: AAD04207.1 .
    AK016550 mRNA. Translation: BAB30298.1 .
    U22057 mRNA. Translation: AAA74921.1 .
    CCDSi CCDS36959.1.
    RefSeqi NP_033748.2. NM_009618.2.
    UniGenei Mm.422850.

    3D structure databases

    ProteinModelPortali Q60718.
    SMRi Q60718. Positions 185-610.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M12.950.

    PTM databases

    PhosphoSitei Q60718.

    Proteomic databases

    PaxDbi Q60718.
    PRIDEi Q60718.

    Protocols and materials databases

    DNASUi 11495.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022618 ; ENSMUSP00000022618 ; ENSMUSG00000022039 .
    GeneIDi 11495.
    KEGGi mmu:11495.
    UCSCi uc007uju.1. mouse.

    Organism-specific databases

    CTDi 2515.
    MGIi MGI:1340894. Adam2.

    Phylogenomic databases

    eggNOGi NOG242228.
    GeneTreei ENSGT00750000117474.
    HOGENOMi HOG000230883.
    HOVERGENi HBG103628.
    InParanoidi Q60718.
    KOi K06833.
    OMAi CGKLICK.
    OrthoDBi EOG7HB58N.
    PhylomeDBi Q60718.
    TreeFami TF314733.

    Enzyme and pathway databases

    Reactomei REACT_199072. Interaction With The Zona Pellucida.

    Miscellaneous databases

    NextBioi 278874.
    PROi Q60718.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q60718.
    CleanExi MM_ADAM2.
    Genevestigatori Q60718.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProi IPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view ]
    Pfami PF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view ]
    SMARTi SM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57552. SSF57552. 1 hit.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse sperm-egg plasma membrane interactions: analysis of roles of egg integrins and the mouse sperm homologue of PH-30 (fertilin) beta."
      Evans J.P., Schultz R.M., Kopf G.S.
      J. Cell Sci. 108:3267-3278(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. Gupta S.K., Alves K., Palladino L.O., Mark G.E., Hollis G.F.
      Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    4. "ADAM, a widely distributed and developmentally regulated gene family encoding membrane proteins with a disintegrin and metalloprotease domain."
      Wolfsberg T.G., Straight P.D., Gerena R.L., Huovila A.-P., Primakoff P., Myles D.G., White J.M.
      Dev. Biol. 169:378-383(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-735.
      Tissue: Testis.

    Entry informationi

    Entry nameiADAM2_MOUSE
    AccessioniPrimary (citable) accession number: Q60718
    Secondary accession number(s): Q60814, Q9D4G3, Q9QWJ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2003
    Last sequence update: February 1, 2003
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3