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Protein

Disintegrin and metalloproteinase domain-containing protein 2

Gene

Adam2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • adult behavior Source: MGI
  • cell adhesion Source: MGI
  • single fertilization Source: InterPro
  • visual learning Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-1300644. Interaction With The Zona Pellucida.

Protein family/group databases

MEROPSiM12.950.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 2
Short name:
ADAM 2
Alternative name(s):
Fertilin subunit beta
PH-30
Short name:
PH30
PH30-beta
Gene namesi
Name:Adam2
Synonyms:Ftnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1340894. Adam2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 686ExtracellularSequence analysisAdd BLAST668
Transmembranei687 – 707HelicalSequence analysisAdd BLAST21
Topological domaini708 – 735CytoplasmicSequence analysisAdd BLAST28

GO - Cellular componenti

  • cell surface Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002904619 – 180By similarityAdd BLAST162
ChainiPRO_0000029047181 – 735Disintegrin and metalloproteinase domain-containing protein 2Add BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi128N-linked (GlcNAc...)Sequence analysis1
Glycosylationi226N-linked (GlcNAc...)Sequence analysis1
Glycosylationi279N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi293 ↔ 376By similarity
Disulfide bondi335 ↔ 360By similarity
Disulfide bondi337 ↔ 342By similarity
Glycosylationi359N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi449 ↔ 469By similarity
Glycosylationi463N-linked (GlcNAc...)Sequence analysis1
Glycosylationi489N-linked (GlcNAc...)Sequence analysis1
Glycosylationi569N-linked (GlcNAc...)Sequence analysis1
Glycosylationi585N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi619 ↔ 630By similarity
Disulfide bondi624 ↔ 636By similarity
Disulfide bondi638 ↔ 647By similarity
Modified residuei729PhosphoserineCombined sources1

Post-translational modificationi

The signal and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ60718.
PRIDEiQ60718.

PTM databases

iPTMnetiQ60718.
PhosphoSitePlusiQ60718.

Expressioni

Tissue specificityi

Expressed specifically in testis.

Gene expression databases

BgeeiENSMUSG00000022039.
CleanExiMM_ADAM2.
GenevisibleiQ60718. MM.

Interactioni

Subunit structurei

Heterodimer with ADAM1/fertilin subunit alpha.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022618.

Structurei

3D structure databases

ProteinModelPortaliQ60718.
SMRiQ60718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini184 – 381Peptidase M12BPROSITE-ProRule annotationAdd BLAST198
Domaini389 – 476DisintegrinPROSITE-ProRule annotationAdd BLAST88
Domaini615 – 648EGF-likePROSITE-ProRule annotationAdd BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi480 – 611Cys-richAdd BLAST132

Domaini

A tripeptide motif (QDE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.By similarity

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00800000124044.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ60718.
KOiK06833.
OMAiCGKLICK.
OrthoDBiEOG091G02F0.
PhylomeDBiQ60718.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR033958. ADAM2.
IPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PANTHERiPTHR11905:SF108. PTHR11905:SF108. 1 hit.
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLILLLLSG LSELGGLSQS QTEGTREKLH VQVTVPEKIR SVTSNGYETQ
60 70 80 90 100
VTYNLKIEGK TYTLDLMQKP FLPPNFRVYS YDNAGIMRSL EQKFQNICYF
110 120 130 140 150
QGYIEGYPNS MVIVSTCTGL RGFLQFGNVS YGIEPLESSS GFEHVIYQVE
160 170 180 190 200
PEKGGALLYA EKDIDLRDSQ YKIRSIKPQR IVSHYLEIHI VVEKQMFEHI
210 220 230 240 250
GADTAIVTQK IFQLIGLANA IFAPFNLTVI LSSLEFWMDE NKILTTGDAN
260 270 280 290 300
KLLYRFLKWK QSYLVLRPHD MAFLLVYRNT TDYVGATYQG KMCDKNYAGG
310 320 330 340 350
VALHPKAVTL ESLAIILVQL LSLSMGLAYD DVNKCQCGVP VCVMNPEAPH
360 370 380 390 400
SSGVRAFSNC SMEDFSKFIT SQSSHCLQNQ PRLQPSYKMA VCGNGEVEED
410 420 430 440 450
EICDCGKKGC AEMPPPCCNP DTCKLSDGSE CSSGICCNSC KLKRKGEVCR
460 470 480 490 500
LAQDECDVTE YCNGTSEVCE DFFVQNGHPC DNRKWICING TCQSGEQQCQ
510 520 530 540 550
DLFGIDAGFG SSECFWELNS KSDISGSCGI SAGGYKECPP NDRMCGKIIC
560 570 580 590 600
KYQSENILKL RSATVIYANI SGHVCVSLEY PQGHNESQKM WVRDGTVCGS
610 620 630 640 650
NKVCQNQKCV ADTFLGYDCN LEKCNHHGVC NNKKNCHCDP TYLPPDCKRM
660 670 680 690 700
KDSYPGGSID SGNKERAEPI PVRPYIASAY RSKSPRWPFF LIIPFYVVIL
710 720 730
VLIGMLVKVY SQRMKWRMDD FSSEEQFESE SESKD
Length:735
Mass (Da):82,375
Last modified:February 1, 2003 - v2
Checksum:i75EC8529CF5B8E2B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2W → R in AAD04207 (Ref. 2) Curated1
Sequence conflicti17 – 20LSQS → IRHE in AAA74921 (PubMed:7750654).Curated4
Sequence conflicti24 – 25GT → A in AAA74921 (PubMed:7750654).Curated2
Sequence conflicti113I → M in AAA90980 (PubMed:7593287).Curated1
Sequence conflicti234 – 242LEFWMDENK → WNFGWMKQ in AAA74921 (PubMed:7750654).Curated9
Sequence conflicti246 – 247TG → QA in AAA74921 (PubMed:7750654).Curated2
Sequence conflicti286A → L in AAA74921 (PubMed:7750654).Curated1
Sequence conflicti331 – 332DV → RRL in AAA74921 (PubMed:7750654).Curated2
Sequence conflicti382R → T in AAD04207 (Ref. 2) Curated1
Sequence conflicti658S → T in AAA74921 (PubMed:7750654).Curated1
Sequence conflicti679A → R in AAD04207 (Ref. 2) Curated1
Sequence conflicti712Q → P in AAA90980 (PubMed:7593287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16242 mRNA. Translation: AAA90980.1.
U38806 mRNA. Translation: AAD04207.1.
AK016550 mRNA. Translation: BAB30298.1.
U22057 mRNA. Translation: AAA74921.1.
CCDSiCCDS36959.1.
RefSeqiNP_033748.2. NM_009618.3.
UniGeneiMm.422850.

Genome annotation databases

EnsembliENSMUST00000022618; ENSMUSP00000022618; ENSMUSG00000022039.
GeneIDi11495.
KEGGimmu:11495.
UCSCiuc007uju.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16242 mRNA. Translation: AAA90980.1.
U38806 mRNA. Translation: AAD04207.1.
AK016550 mRNA. Translation: BAB30298.1.
U22057 mRNA. Translation: AAA74921.1.
CCDSiCCDS36959.1.
RefSeqiNP_033748.2. NM_009618.3.
UniGeneiMm.422850.

3D structure databases

ProteinModelPortaliQ60718.
SMRiQ60718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022618.

Protein family/group databases

MEROPSiM12.950.

PTM databases

iPTMnetiQ60718.
PhosphoSitePlusiQ60718.

Proteomic databases

PaxDbiQ60718.
PRIDEiQ60718.

Protocols and materials databases

DNASUi11495.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022618; ENSMUSP00000022618; ENSMUSG00000022039.
GeneIDi11495.
KEGGimmu:11495.
UCSCiuc007uju.1. mouse.

Organism-specific databases

CTDi2515.
MGIiMGI:1340894. Adam2.

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00800000124044.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ60718.
KOiK06833.
OMAiCGKLICK.
OrthoDBiEOG091G02F0.
PhylomeDBiQ60718.
TreeFamiTF314733.

Enzyme and pathway databases

ReactomeiR-MMU-1300644. Interaction With The Zona Pellucida.

Miscellaneous databases

PROiQ60718.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022039.
CleanExiMM_ADAM2.
GenevisibleiQ60718. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR033958. ADAM2.
IPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PANTHERiPTHR11905:SF108. PTHR11905:SF108. 1 hit.
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADAM2_MOUSE
AccessioniPrimary (citable) accession number: Q60718
Secondary accession number(s): Q60814, Q9D4G3, Q9QWJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: February 1, 2003
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.