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Reviewed, UniProtKB/Swiss-Prot Q60716 (P4HA2_MOUSE)

Last modified November 25, 2008. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Prolyl 4-hydroxylase subunit alpha-2
    EC=1.14.11.2
Alternative name(s):
    4-PH alpha-2
    Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2
Gene names
Name: P4ha2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length537 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Catalytic activity

Procollagen L-proline + 2-oxoglutarate + O(2) = procollagen trans-4-hydroxy-L-proline + succinate + CO(2).

Cofactor

Binds 1 Fe(2+) ion per subunit.

Ascorbate.

Enzyme regulation

In contrast to the subunit alpha-1, alpha-2 is inhibited by poly(L-proline) only at very high concentrations.

Subunit structure

Heterotetramer of two alpha-2 chains and two beta chains (the beta chain is the multi-functional PDI).

Subcellular location

Endoplasmic reticulum lumen.

Tissue specificity

Expressed in a variety of tissues.

Sequence similarities

Belongs to the P4HA family.

Contains 1 PKHD (prolyl/lysyl hydroxylase) domain.

Contains 1 TPR repeat.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform IIb (identifier: Q60716-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform IIa (identifier: Q60716-2)

The sequence of this isoform differs from the canonical sequence as follows:
     438-453: SDDEDAFKRLGTGNRV → RPFDSGLKTEGNRL

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 537514Prolyl 4-hydroxylase subunit alpha-2
PRO_0000022727

Regions

Repeat209 – 24234TPR
Domain338 – 521184PKHD

Sites

Metal binding4321Iron By similarity
Metal binding4341Iron By similarity
Metal binding5031Iron By similarity
Binding site51312-oxoglutarate Potential

Amino acid modifications

Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation2661N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence438 – 45316SDDED…TGNRV → RPFDSGLKTEGNRL in isoform IIa.
VSP_004507

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Sequences

Sequence LengthMass (Da)Tools
Isoform IIb [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C47D976C75E82518

FASTA53761,002
        10         20         30         40         50         60 
MKLQVLVLVL LMSWFGVLSW VQAEFFTSIG HMTDLIYAEK DLVQSLKEYI LVEEAKLAKI 

        70         80         90        100        110        120 
KSWASKMEAL TSRSAADPEG YLAHPVNAYK LVKRLNTDWP ALGDLVLQDA SAGFVANLSV 

       130        140        150        160        170        180 
QRQFFPTDED ESGAARALMR LQDTYKLDPD TISRGELPGT KYQAMLSVDD CFGLGRSAYN 

       190        200        210        220        230        240 
EGDYYHTVLW MEQVLKQLDA GEEATVTKSL VLDYLSYAVF QLGDLHRAVE LTRRLLSLDP 

       250        260        270        280        290        300 
SHERAGGNLR YFERLLEEER GKSLSNQTDA GLATQENLYE RPTDYLPERD VYESLCRGEG 

       310        320        330        340        350        360 
VKLTPRRQKK LFCRYHHGNR VPQLLIAPFK EEDEWDSPHI VRYYDVMSDE EIERIKEIAK 

       370        380        390        400        410        420 
PKLARATVRD PKTGVLTVAS YRVSKSSWLE EDDDPVVARV NRRMQHITGL TVKTAELLQV 

       430        440        450        460        470        480 
ANYGMGGQYE PHFDFSRSDD EDAFKRLGTG NRVATFLNYM SDVEAGGATV FPDLGAAIWP 

       490        500        510        520        530 
KKGTAVFWYN LLRSGEGDYR TRHAACPVLV GCKWVSNKWF HERGQEFLRP CGTTEVD

« Hide

Isoform IIa [UniParc].

Checksum: BBA7883CD43CB996
Show »

53560,812

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References

« Hide 'large scale' references
[1]"Cloning, baculovirus expression, and characterization of a second mouse prolyl 4-hydroxylase alpha-subunit isoform: formation of an alpha 2 beta 2 tetramer with the protein disulfide-isomerase/beta subunit."
Helaakoski T., Annunen P., Vuori K., Macneil I.A., Pihlajaniemi T., Kivirikko K.I.
Proc. Natl. Acad. Sci. U.S.A. 92:4427-4431(1995) [PubMed: 7753822] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB).
[2]"Characterization of the human and mouse genes for the alpha subunit of type II prolyl 4-hydroxylase. Identification of a previously unknown alternatively spliced exon and its expression in various tissues."
Nokelainen M., Nissi R., Kukkola L., Helaakoski T., Myllyharju J.
Eur. J. Biochem. 268:5300-5309(2001) [PubMed: 11606192] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS IIA AND IIB).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IIA).
+Additional computationally mapped references.

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Cross-references

Sequence databases

U16163 mRNA. Translation: AAC52198.1.
AJ314858 Genomic DNA. Translation: CAC85690.1.
AJ314858 Genomic DNA. Translation: CAC85691.1.
BC018411 mRNA. Translation: AAH18411.1.
PIRI49135.
RefSeqNP_035161.1.
UniGeneMm.3705

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSMUSG00000018906. Mus musculus. [Contig view]
GeneID18452.
KEGGmmu:18452.

Organism-specific databases

MGIMGI:894286. P4ha2.

Phylogenomic databases

HOGENOMQ60716.
HOVERGENQ60716.

Gene expression databases

ArrayExpressQ60716.
GermOnlineENSMUSG00000018906. Mus musculus.

Family and domain databases

InterProIPR005123. 2OG-FeII_Oase.
IPR006620. Pro_4_hyd_alph.
IPR013547. Pro_4_hyd_alph_N.
IPR011990. TPR-like_helical.
IPR013026. TPR_region.
[Graphical view]
Gene3DG3DSA:1.25.40.10. TPR-like_helical. 1 hit.
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF08336. P4Ha_N. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS50005. TPR. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio294140.
SOURCESearch...

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Entry information

Entry nameP4HA2_MOUSE
AccessionPrimary (citable) accession number: Q60716
Secondary accession number(s): Q8VBU4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents