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Q60714

- S27A1_MOUSE

UniProt

Q60714 - S27A1_MOUSE

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Protein

Long-chain fatty acid transport protein 1

Gene

Slc27a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. The LFCA import appears to be hormone-regulated in a tissue-specific manner. In adipocytes, but not myocytes, insulin induces a rapid translocation of FatP1 from intracellular compartments to the plasma membrane, paralleled by increased LFCA uptake. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LFCA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi246 – 25712AMPCuratedAdd
BLAST

GO - Molecular functioni

  1. fatty acid transporter activity Source: MGI
  2. long-chain fatty acid-CoA ligase activity Source: MGI
  3. nucleotide binding Source: UniProtKB-KW
  4. protein homodimerization activity Source: MGI
  5. very long-chain fatty acid-CoA ligase activity Source: MGI

GO - Biological processi

  1. adiponectin-activated signaling pathway Source: MGI
  2. cardiolipin biosynthetic process Source: Ensembl
  3. fatty acid transport Source: MGI
  4. long-chain fatty acid metabolic process Source: MGI
  5. long-chain fatty acid transport Source: MGI
  6. medium-chain fatty acid transport Source: MGI
  7. negative regulation of phospholipid biosynthetic process Source: Ensembl
  8. phosphatidic acid biosynthetic process Source: Ensembl
  9. phosphatidylcholine biosynthetic process Source: Ensembl
  10. phosphatidylethanolamine biosynthetic process Source: Ensembl
  11. phosphatidylinositol biosynthetic process Source: Ensembl
  12. phosphatidylserine biosynthetic process Source: Ensembl
  13. positive regulation of heat generation Source: MGI
  14. positive regulation of protein serine/threonine kinase activity Source: MGI
  15. response to cold Source: MGI
  16. response to insulin Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism, Lipid transport, Transport

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198602. PPARA activates gene expression.
REACT_198636. Transport of fatty acids.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain fatty acid transport protein 1 (EC:6.2.1.-)
Short name:
FATP-1
Short name:
Fatty acid transport protein 1
Alternative name(s):
Solute carrier family 27 member 1
Gene namesi
Name:Slc27a1
Synonyms:Fatp, Fatp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1347098. Slc27a1.

Subcellular locationi

Cell membrane; Single-pass membrane protein. Endomembrane system; Single-pass membrane protein. Cytoplasm
Note: Plasma membrane and intracellular membranes, at least in adipocytes. Predominantly cytoplasmic in myocytes.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313ExtracellularSequence AnalysisAdd
BLAST
Transmembranei14 – 3421HelicalSequence AnalysisAdd
BLAST
Topological domaini35 – 646612CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasmic vesicle Source: MGI
  2. endoplasmic reticulum Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi249 – 2546TSGTTG → LEAAA: Abolishes very-long-chain acyl-CoA synthetase activity. 1 Publication
Mutagenesisi250 – 2501S → A: Diminishes LCFA import and decreases nucleotide binding. 2 Publications
Mutagenesisi252 – 2521T → A: Diminishes LCFA import and decreases nucleotide binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 646646Long-chain fatty acid transport protein 1PRO_0000193202Add
BLAST

Proteomic databases

MaxQBiQ60714.
PaxDbiQ60714.
PRIDEiQ60714.

PTM databases

PhosphoSiteiQ60714.

Expressioni

Tissue specificityi

Highest expression in skeletal muscle, heart and fat. Lower levels in brain, kidney, lung and liver. No expression in spleen or intestine.1 Publication

Gene expression databases

BgeeiQ60714.
ExpressionAtlasiQ60714. baseline and differential.
GenevestigatoriQ60714.

Interactioni

Subunit structurei

Self-associates. May function as a homodimer.

Protein-protein interaction databases

IntActiQ60714. 1 interaction.
MINTiMINT-4094970.

Structurei

3D structure databases

ProteinModelPortaliQ60714.
SMRiQ60714. Positions 77-607.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 475285Sufficient for oligomerizationAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0318.
GeneTreeiENSGT00550000074420.
HOGENOMiHOG000044189.
HOVERGENiHBG005642.
InParanoidiQ60714.
KOiK08745.
OMAiIWEEFTE.
OrthoDBiEOG7W6WKB.
PhylomeDBiQ60714.
TreeFamiTF313430.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60714-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRAPGAGTAS VASLALLWFL GLPWTWSAAA AFCVYVGGGG WRFLRIVCKT
60 70 80 90 100
ARRDLFGLSV LIRVRLELRR HRRAGDTIPC IFQAVARRQP ERLALVDASS
110 120 130 140 150
GICWTFAQLD TYSNAVANLF RQLGFAPGDV VAVFLEGRPE FVGLWLGLAK
160 170 180 190 200
AGVVAALLNV NLRREPLAFC LGTSAAKALI YGGEMAAAVA EVSEQLGKSL
210 220 230 240 250
LKFCSGDLGP ESILPDTQLL DPMLAEAPTT PLAQAPGKGM DDRLFYIYTS
260 270 280 290 300
GTTGLPKAAI VVHSRYYRIA AFGHHSYSMR AADVLYDCLP LYHSAGNIMG
310 320 330 340 350
VGQCVIYGLT VVLRKKFSAS RFWDDCVKYN CTVVQYIGEI CRYLLRQPVR
360 370 380 390 400
DVEQRHRVRL AVGNGLRPAI WEEFTQRFGV PQIGEFYGAT ECNCSIANMD
410 420 430 440 450
GKVGSCGFNS RILTHVYPIR LVKVNEDTME PLRDSEGLCI PCQPGEPGLL
460 470 480 490 500
VGQINQQDPL RRFDGYVSDS ATNKKIAHSV FRKGDSAYLS GDVLVMDELG
510 520 530 540 550
YMYFRDRSGD TFRWRGENVS TTEVEAVLSR LLGQTDVAVY GVAVPGVEGK
560 570 580 590 600
AGMAAIADPH SQLDPNSMYQ ELQKVLASYA RPIFLRLLPQ VDTTGTFKIQ
610 620 630 640
KTRLQREGFD PRQTSDRLFF LDLKQGRYVP LDERVHARIC AGDFSL
Length:646
Mass (Da):71,276
Last modified:November 1, 1996 - v1
Checksum:i910B92BA8D985B4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15976 mRNA. Translation: AAC71060.1.
AF023258, AF023256, AF023257 Genomic DNA. Translation: AAC69640.1.
BC028937 mRNA. Translation: AAH28937.1.
CCDSiCCDS40386.1.
PIRiA55093.
RefSeqiNP_036107.1. NM_011977.3.
XP_006509736.1. XM_006509673.1.
XP_006509737.1. XM_006509674.1.
XP_006509738.1. XM_006509675.1.
XP_006509739.1. XM_006509676.1.
UniGeneiMm.38165.

Genome annotation databases

EnsembliENSMUST00000034267; ENSMUSP00000034267; ENSMUSG00000031808.
GeneIDi26457.
KEGGimmu:26457.
UCSCiuc009mdw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15976 mRNA. Translation: AAC71060.1 .
AF023258 , AF023256 , AF023257 Genomic DNA. Translation: AAC69640.1 .
BC028937 mRNA. Translation: AAH28937.1 .
CCDSi CCDS40386.1.
PIRi A55093.
RefSeqi NP_036107.1. NM_011977.3.
XP_006509736.1. XM_006509673.1.
XP_006509737.1. XM_006509674.1.
XP_006509738.1. XM_006509675.1.
XP_006509739.1. XM_006509676.1.
UniGenei Mm.38165.

3D structure databases

ProteinModelPortali Q60714.
SMRi Q60714. Positions 77-607.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q60714. 1 interaction.
MINTi MINT-4094970.

Chemistry

BindingDBi Q60714.
ChEMBLi CHEMBL2052039.

PTM databases

PhosphoSitei Q60714.

Proteomic databases

MaxQBi Q60714.
PaxDbi Q60714.
PRIDEi Q60714.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034267 ; ENSMUSP00000034267 ; ENSMUSG00000031808 .
GeneIDi 26457.
KEGGi mmu:26457.
UCSCi uc009mdw.1. mouse.

Organism-specific databases

CTDi 376497.
MGIi MGI:1347098. Slc27a1.

Phylogenomic databases

eggNOGi COG0318.
GeneTreei ENSGT00550000074420.
HOGENOMi HOG000044189.
HOVERGENi HBG005642.
InParanoidi Q60714.
KOi K08745.
OMAi IWEEFTE.
OrthoDBi EOG7W6WKB.
PhylomeDBi Q60714.
TreeFami TF313430.

Enzyme and pathway databases

Reactomei REACT_198602. PPARA activates gene expression.
REACT_198636. Transport of fatty acids.

Miscellaneous databases

ChiTaRSi Slc27a1. mouse.
NextBioi 304565.
PROi Q60714.
SOURCEi Search...

Gene expression databases

Bgeei Q60714.
ExpressionAtlasi Q60714. baseline and differential.
Genevestigatori Q60714.

Family and domain databases

InterProi IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein."
    Schaffer J.E., Lodish H.F.
    Cell 79:427-436(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss.
  2. "Characterization of the murine fatty acid transport protein gene and its insulin response sequence."
    Hui T.Y., Frohnert B.I., Smith A.J., Schaffer J.E., Bernlohr D.A.
    J. Biol. Chem. 273:27420-27429(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  4. "Substitution of alanine for serine 250 in the murine fatty acid transport protein inhibits long chain fatty acid transport."
    Stuhlsatz-Krouper S.M., Bennett N.E., Schaffer J.E.
    J. Biol. Chem. 273:28642-28650(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-250.
  5. "A family of fatty acid transporters conserved from mycobacterium to man."
    Hirsch D., Stahl A., Lodish H.F.
    Proc. Natl. Acad. Sci. U.S.A. 95:8625-8629(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN FATTY ACID TRANSPORT, TISSUE SPECIFICITY.
  6. "The fatty acid transport protein (FATP1) is a very long chain acyl-CoA synthetase."
    Coe N.R., Smith A.J., Frohnert B.I., Watkins P.A., Bernlohr D.A.
    J. Biol. Chem. 274:36300-36304(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ACYL-COA LIGASE, SUBCELLULAR LOCATION, MUTAGENESIS OF 249-THR--GLY-254.
  7. "Molecular aspects of fatty acid transport: mutations in the IYTSGTTGXPK motif impair fatty acid transport protein function."
    Stuhlsatz-Krouper S.M., Bennett N.E., Schaffer J.E.
    Prostaglandins Leukot. Essent. Fatty Acids 60:285-289(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-250 AND THR-252.
  8. "Membrane topology of the murine fatty acid transport protein 1."
    Lewis S.E., Listenberger L.L., Ory D.S., Schaffer J.E.
    J. Biol. Chem. 276:37042-37050(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
  9. Cited for: OLIGOMERIZATION.
  10. "Insulin causes fatty acid transport protein translocation and enhanced fatty acid uptake in adipocytes."
    Stahl A., Evans J.G., Pattel S., Hirsch D., Lodish H.F.
    Dev. Cell 2:477-488(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Characterization of the acyl-CoA synthetase activity of purified murine fatty acid transport protein 1."
    Hall A.M., Smith A.J., Bernlohr D.A.
    J. Biol. Chem. 278:43008-43013(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ACYL-COA LIGASE.
  12. Cited for: FUNCTION.
  13. "Impact on fatty acid metabolism and differential localization of FATP1 and FAT/CD36 proteins delivered in cultured human muscle cells."
    Garcia-Martinez C., Marotta M., Moore-Carrasco R., Guitart M., Camps M., Busquets S., Montell E., Gomez-Foix A.M.
    Am. J. Physiol. 288:C1264-C1272(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  14. "Comparative biochemical studies of the murine fatty acid transport proteins (FATP) expressed in yeast."
    DiRusso C.C., Li H., Darwis D., Watkins P.A., Berger J., Black P.N.
    J. Biol. Chem. 280:16829-16837(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiS27A1_MOUSE
AccessioniPrimary (citable) accession number: Q60714
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mice deficient for Fatp1 are protected from fat-induced insulin resistance and intramuscular accumulation of fatty acyl-CoA without alteration in whole-body adiposity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3