ID TBX2_MOUSE Reviewed; 711 AA. AC Q60707; Q5SSP7; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 27-JUL-2011, sequence version 3. DT 27-MAR-2024, entry version 175. DE RecName: Full=T-box transcription factor TBX2; DE Short=T-box protein 2; GN Name=Tbx2; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE. RC TISSUE=Embryo; RX PubMed=7920656; DOI=10.1038/ng0794-383; RA Bollag R.J., Siegfried Z., Cebra-Thomas J.A., Garvey N., Davison E.M., RA Silver L.M.; RT "An ancient family of embryonically expressed mouse genes sharing a RT conserved protein motif with the T locus."; RL Nat. Genet. 7:383-389(1994). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=10770922; DOI=10.1074/jbc.m000035200; RA Carreira S., Liu B., Goding C.R.; RT "The gene encoding the T-box Factor Tbx2 is a target for the RT microphthalmia-associated transcription factor in melanocytes."; RL J. Biol. Chem. 275:21920-21927(2000). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [6] RP DEVELOPMENTAL STAGE. RX PubMed=8853987; RX DOI=10.1002/(sici)1097-0177(199608)206:4<379::aid-aja4>3.0.co;2-f; RA Chapman D.L., Garvey N., Hancock S., Alexiou M., Agulnik S.I., RA Gibson-Brown J.J., Cebra-Thomas J., Bollag R.J., Silver L.M., RA Papaioannou V.E.; RT "Expression of the T-box family genes, Tbx1-Tbx5, during early mouse RT development."; RL Dev. Dyn. 206:379-390(1996). RN [7] RP FUNCTION. RX PubMed=9710594; DOI=10.1128/mcb.18.9.5099; RA Carreira S., Dexter T.J., Yavuzer U., Easty D.J., Goding C.R.; RT "Brachyury-related transcription factor Tbx2 and repression of the RT melanocyte-specific TRP-1 promoter."; RL Mol. Cell. Biol. 18:5099-5108(1998). RN [8] RP FUNCTION. RX PubMed=11867218; DOI=10.1016/s0378-1119(01)00878-2; RA Paxton C., Zhao H., Chin Y., Langner K., Reecy J.; RT "Murine Tbx2 contains domains that activate and repress gene RT transcription."; RL Gene 283:117-124(2002). RN [9] RP FUNCTION. RX PubMed=12023302; DOI=10.1101/gad.222902; RA Habets P.E., Moorman A.F., Clout D.E., van Roon M.A., Lingbeek M., RA van Lohuizen M., Campione M., Christoffels V.M.; RT "Cooperative action of Tbx2 and Nkx2.5 inhibits ANF expression in the RT atrioventricular canal: implications for cardiac chamber formation."; RL Genes Dev. 16:1234-1246(2002). RN [10] RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE. RX PubMed=15459098; DOI=10.1242/dev.01378; RA Harrelson Z., Kelly R.G., Goldin S.N., Gibson-Brown J.J., Bollag R.J., RA Silver L.M., Papaioannou V.E.; RT "Tbx2 is essential for patterning the atrioventricular canal and for RT morphogenesis of the outflow tract during heart development."; RL Development 131:5041-5052(2004). RN [11] RP FUNCTION, AND DEVELOPMENTAL STAGE. RX PubMed=16222716; DOI=10.1002/dvdy.20575; RA Jerome-Majewska L.A., Jenkins G.P., Ernstoff E., Zindy F., Sherr C.J., RA Papaioannou V.E.; RT "Tbx3, the ulnar-mammary syndrome gene, and Tbx2 interact in mammary gland RT development through a p19Arf/p53-independent pathway."; RL Dev. Dyn. 234:922-933(2005). RN [12] RP FUNCTION, MUTAGENESIS OF SER-336; SER-623 AND SER-675, AND PHOSPHORYLATION RP AT SER-336; SER-623 AND SER-675. RX PubMed=18025091; DOI=10.1074/jbc.m705651200; RA Abrahams A., Mowla S., Parker M.I., Goding C.R., Prince S.; RT "UV-mediated regulation of the anti-senescence factor Tbx2."; RL J. Biol. Chem. 283:2223-2230(2008). RN [13] RP FUNCTION. RX PubMed=22186728; DOI=10.1242/dev.067058; RA Sakabe M., Kokubo H., Nakajima Y., Saga Y.; RT "Ectopic retinoic acid signaling affects outflow tract cushion development RT through suppression of the myocardial Tbx2-Tgfbeta2 pathway."; RL Development 139:385-395(2012). RN [14] RP FUNCTION, AND DEVELOPMENTAL STAGE. RX PubMed=26486273; DOI=10.1111/cpr.12227; RA Pan L., Ma X., Wen B., Su Z., Zheng X., Liu Y., Li H., Chen Y., Wang J., RA Lu F., Qu J., Hou L.; RT "Microphthalmia-associated transcription factor/T-box factor-2 axis acts RT through Cyclin D1 to regulate melanocyte proliferation."; RL Cell Prolif. 48:631-642(2015). RN [15] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=27720610; DOI=10.1016/j.devcel.2016.08.007; RA Luedtke T.H., Rudat C., Wojahn I., Weiss A.C., Kleppa M.J., Kurz J., RA Farin H.F., Moon A., Christoffels V.M., Kispert A.; RT "Tbx2 and Tbx3 Act Downstream of Shh to Maintain Canonical Wnt Signaling RT during Branching Morphogenesis of the Murine Lung."; RL Dev. Cell 39:239-253(2016). RN [16] RP FUNCTION. RX PubMed=26971330; DOI=10.1007/s11010-016-2680-7; RA Chen Y., Pan L., Su Z., Wang J., Li H., Ma X., Liu Y., Lu F., Qu J., RA Hou L.; RT "The transcription factor TBX2 regulates melanogenesis in melanocytes by RT repressing Oca2."; RL Mol. Cell. Biochem. 415:103-109(2016). RN [17] RP FUNCTION, AND DEVELOPMENTAL STAGE. RX PubMed=28910203; DOI=10.1080/02713683.2017.1338351; RA Wang J., Liu Y., Su Z., Pan L., Lu F., Qu J., Hou L.; RT "The T-Box Transcription Factor TBX2 Regulates Cell Proliferation in the RT Retinal Pigment Epithelium."; RL Curr. Eye Res. 42:1537-1544(2017). RN [18] RP FUNCTION, AND MUTAGENESIS OF 132-ARG-ARG-133. RX PubMed=30599067; DOI=10.1007/s00018-018-2998-2; RA Reinhardt S., Schuck F., Stoye N., Hartmann T., Grimm M.O.W., RA Pflugfelder G., Endres K.; RT "Transcriptional repression of the ectodomain sheddase ADAM10 by TBX2 and RT potential implication for Alzheimer's disease."; RL Cell. Mol. Life Sci. 76:1005-1025(2019). RN [19] RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE. RX PubMed=33795231; DOI=10.1242/dev.195651; RA Kaiser M., Wojahn I., Rudat C., Luedtke T.H., Christoffels V.M., Moon A., RA Kispert A., Trowe M.O.; RT "Regulation of otocyst patterning by Tbx2 and Tbx3 is required for inner RT ear morphogenesis in the mouse."; RL Development 148:0-0(2021). RN [20] RP FUNCTION, INTERACTION WITH CHD4; HDAC1; HDAC2; CBX3; HMGB2 AND PBX1, RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE. RX PubMed=33731112; DOI=10.1186/s12931-021-01679-y; RA Luedtke T.H., Wojahn I., Kleppa M.J., Schierstaedt J., Christoffels V.M., RA Kuenzler P., Kispert A.; RT "Combined genomic and proteomic approaches reveal DNA binding sites and RT interaction partners of TBX2 in the developing lung."; RL Respir. Res. 22:85-85(2021). RN [21] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-336; SER-342 AND SER-360, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Kidney, and Lung; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). CC -!- FUNCTION: Transcription factor which acts as a transcriptional CC repressor (PubMed:22186728, PubMed:11867218, PubMed:18025091, CC PubMed:12023302). May also function as a transcriptional activator CC (PubMed:26486273, PubMed:22186728, PubMed:11867218). Binds to the CC palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence, or a half- CC site, which are present in the regulatory region of several genes CC (PubMed:9710594, PubMed:26971330, PubMed:12023302, PubMed:33731112, CC PubMed:27720610). Required for cardiac atrioventricular canal formation CC (PubMed:15459098). May cooperate with NKX2.5 to negatively modulate CC expression of NPPA/ANF in the atrioventricular canal (PubMed:12023302). CC May play a role as a positive regulator of TGFB2 expression, perhaps CC acting in concert with GATA4 in the developing outflow tract myocardium CC (PubMed:22186728). Plays a role in limb pattern formation CC (PubMed:15459098). Acts as a transcriptional repressor of ADAM10 gene CC expression, perhaps in concert with histone deacetylase HDAC1 as CC cofactor (PubMed:30599067). Involved in branching morphogenesis in both CC developing lungs and adult mammary glands, via negative modulation of CC target genes; acting redundantly with TBX3 (PubMed:27720610, CC PubMed:16222716). Required, together with TBX3, to maintain cell CC proliferation in the embryonic lung mesenchyme; perhaps acting CC downstream of SHH, BMP and TGFbeta signaling (PubMed:27720610). CC Involved in modulating early inner ear development, acting CC independently of, and also redundantly with TBX3, in different CC subregions of the developing ear (PubMed:33795231). Acts as a negative CC regulator of PML function in cellular senescence (By similarity). Acts CC as a negative regulator of expression of CDKN1A/p21, IL33 and CCN4; CC repression of CDKN1A is enhanced in response to UV-induced stress, CC perhaps as a result of phosphorylation by p38 MAPK (PubMed:18025091, CC PubMed:33731112). Negatively modulates expression of CDKN2A/p19ARF and CC CDH1/E-cadherin (By similarity). Plays a role in induction of the CC epithelial-mesenchymal transition (EMT) (By similarity). Plays a role CC in melanocyte proliferation, perhaps via regulation of cyclin CCND1 CC (PubMed:26486273). Involved in melanogenesis, acting via negative CC modulation of expression of DHICA oxidase/TYRP1 and P protein/OCA2 CC (PubMed:26971330, PubMed:9710594). Involved in regulating retinal CC pigment epithelium (RPE) cell proliferation, perhaps via negatively CC modulating transcription of the transcription factor CEBPD CC (PubMed:28910203). {ECO:0000250|UniProtKB:Q13207, CC ECO:0000269|PubMed:11867218, ECO:0000269|PubMed:12023302, CC ECO:0000269|PubMed:15459098, ECO:0000269|PubMed:16222716, CC ECO:0000269|PubMed:18025091, ECO:0000269|PubMed:22186728, CC ECO:0000269|PubMed:26486273, ECO:0000269|PubMed:26971330, CC ECO:0000269|PubMed:27720610, ECO:0000269|PubMed:28910203, CC ECO:0000269|PubMed:30599067, ECO:0000269|PubMed:33731112, CC ECO:0000269|PubMed:33795231, ECO:0000269|PubMed:9710594}. CC -!- SUBUNIT: Binds DNA as a monomer (By similarity). Interacts with CHD4, CC HDAC1 and HDAC2, perhaps as components of a NuRD-like complex CC (PubMed:33731112). Interacts with CBX3, HMGB2 and PBX1 CC (PubMed:33731112). Interacts with PML (By similarity). CC {ECO:0000250|UniProtKB:Q13207, ECO:0000269|PubMed:33731112}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26486273, CC ECO:0000269|PubMed:33731112}. CC -!- TISSUE SPECIFICITY: In adults, highest levels in lung. Also found in CC heart, kidney, and ovary. CC -!- DEVELOPMENTAL STAGE: Expressed in the otic placode at 8.5 dpc (at CC protein level) (PubMed:33795231). Between 10.5-12.0 dpc, expressed in CC various regions of the developing ear, including the cochlear duct, CC endolymphatic duct and the vestibule, but not in the region which gives CC rise to the posterior and anterior semicircular canals (at protein CC level) (PubMed:33795231). Expressed at 8.5 dpc in the cardiac crescent, CC the atrium and the inflow tract (IFT) (PubMed:15459098). Expressed at CC 9.5 dpc in the otic and optic vesicles, facial region, septum CC transversum, bilateral nephrogenic mesodermal cords, ventral body wall CC mesoderm caudal to the forelimbs, pharyngeal arch mesenchyme that CC contains neural crest cells, including those migrating into the outflow CC tract (OFT), septum OFT, inner curvature, atrioventricular canal (AVC) CC and IFT of the heart (PubMed:7920656, PubMed:8853987, PubMed:15459098, CC PubMed:33795231). Expressed in a continuous stripe of mesenchyme in the CC ventro-lateral body wall between the fore and hind limb buds at day CC 10.5-11.5 dpc (PubMed:16222716). At 12.5 dpc, expressed in the CC trigeminal ganglia, facial regions, retina and limb bud mesenchyme CC (PubMed:8853987). In later stages, found in ear pinnae, the milk line, CC lung mesenchyme, body wall, genital ridge and developing nervous system CC (PubMed:8853987, PubMed:33731112). Expressed in proliferating retinal CC pigment epithelium (RPE) cells at 14.5 dpc, and continues after birth, CC but diminishes by postnatal day 90 (PubMed:28910203). Expressed in CC melanocytes of postnatal day 3 hair follicles (PubMed:26486273). CC {ECO:0000269|PubMed:15459098, ECO:0000269|PubMed:16222716, CC ECO:0000269|PubMed:26486273, ECO:0000269|PubMed:28910203, CC ECO:0000269|PubMed:33731112, ECO:0000269|PubMed:33795231, CC ECO:0000269|PubMed:7920656, ECO:0000269|PubMed:8853987}. CC -!- DOMAIN: Repression domain 1 (RD1) is involved in transcriptional CC repression (By similarity). RD1 is necessary for its interaction with CC PML (By similarity). {ECO:0000250|UniProtKB:Q13207}. CC -!- PTM: Phosphorylated (PubMed:18025091). May be phosphorylated by p38 CC MAPK in response to UV irradiation stress (PubMed:18025091). CC {ECO:0000269|PubMed:18025091}. CC -!- DISRUPTION PHENOTYPE: Knockouts do not survive beyond embryonic 14.5 CC dpc (PubMed:15459098). Abnormal atrioventricular morphology at 9.5-10.5 CC dpc and outflow tract (OFT) septation defects in those surviving to CC 12.5 dpc (PubMed:15459098). Hindlimb digit duplication at 14.5 dpc CC (PubMed:15459098). Increased expression of CDKN1A, FRZB, IL33, SHISA3, CC and CCN4/WISP1 in lung mesenchyme between 12.5-14.5 dpc CC (PubMed:33731112). Conditional knockdown targeted mainly to lung CC mesenchyme causes lung hypoplasia at 18.5 dpc (PubMed:27720610). CC Conditional knockdown targeted mainly to the otic epithelium disrupts CC inner ear morphogenesis, which is exacerbated by simultaneous CC conditional knockdown of TBX3 (PubMed:33795231). Simultaneous CC conditional knockdown of TBX2 and TBX3 targeted mainly to lung CC mesenchyme causes severe bleeding from 10.5 dpc and embryos die shortly CC thereafter, perhaps as a result of knockdown in the developing heart CC (PubMed:27720610). {ECO:0000269|PubMed:15459098, CC ECO:0000269|PubMed:27720610, ECO:0000269|PubMed:33731112, CC ECO:0000269|PubMed:33795231}. CC -!- SEQUENCE CAUTION: CC Sequence=AAC52697.1; Type=Frameshift; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U15566; AAC52697.1; ALT_FRAME; mRNA. DR EMBL; AF244917; AAF90050.1; -; Genomic_DNA. DR EMBL; AL596324; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AL662910; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH466556; EDL15775.1; -; Genomic_DNA. DR EMBL; BC156393; -; NOT_ANNOTATED_CDS; mRNA. DR CCDS; CCDS36266.1; -. DR PIR; S46458; S46458. DR RefSeq; NP_033350.2; NM_009324.2. DR AlphaFoldDB; Q60707; -. DR SMR; Q60707; -. DR BioGRID; 203987; 15. DR STRING; 10090.ENSMUSP00000000095; -. DR iPTMnet; Q60707; -. DR PhosphoSitePlus; Q60707; -. DR MaxQB; Q60707; -. DR PaxDb; 10090-ENSMUSP00000000095; -. DR ProteomicsDB; 263016; -. DR Antibodypedia; 1779; 387 antibodies from 36 providers. DR DNASU; 21385; -. DR Ensembl; ENSMUST00000000095.7; ENSMUSP00000000095.7; ENSMUSG00000000093.7. DR GeneID; 21385; -. DR KEGG; mmu:21385; -. DR UCSC; uc007ksb.1; mouse. DR AGR; MGI:98494; -. DR CTD; 6909; -. DR MGI; MGI:98494; Tbx2. DR VEuPathDB; HostDB:ENSMUSG00000000093; -. DR eggNOG; KOG3585; Eukaryota. DR GeneTree; ENSGT00940000158439; -. DR HOGENOM; CLU_023038_1_0_1; -. DR InParanoid; Q60707; -. DR OMA; EQCKPER; -. DR OrthoDB; 5323209at2759; -. DR PhylomeDB; Q60707; -. DR TreeFam; TF106341; -. DR BioGRID-ORCS; 21385; 4 hits in 79 CRISPR screens. DR PRO; PR:Q60707; -. DR Proteomes; UP000000589; Chromosome 11. DR RNAct; Q60707; Protein. DR Bgee; ENSMUSG00000000093; Expressed in urothelium of ureter and 215 other cell types or tissues. DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB. DR GO; GO:0005634; C:nucleus; IDA:UniProtKB. DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI. DR GO; GO:0003677; F:DNA binding; IDA:MGI. DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI. DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. DR GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:UniProtKB. DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI. DR GO; GO:0042826; F:histone deacetylase binding; IPI:UniProtKB. DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB. DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI. DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI. DR GO; GO:0035909; P:aorta morphogenesis; IMP:BHF-UCL. DR GO; GO:0006915; P:apoptotic process; IMP:UniProtKB. DR GO; GO:0036302; P:atrioventricular canal development; IMP:BHF-UCL. DR GO; GO:1905222; P:atrioventricular canal morphogenesis; IGI:BHF-UCL. DR GO; GO:1905072; P:cardiac jelly development; ISO:MGI. DR GO; GO:0060379; P:cardiac muscle cell myoblast differentiation; IDA:BHF-UCL. DR GO; GO:0048738; P:cardiac muscle tissue development; IMP:MGI. DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central. DR GO; GO:0071549; P:cellular response to dexamethasone stimulus; IEA:Ensembl. DR GO; GO:0090398; P:cellular senescence; IDA:MGI. DR GO; GO:0090103; P:cochlea morphogenesis; IMP:UniProtKB. DR GO; GO:0060560; P:developmental growth involved in morphogenesis; IMP:MGI. DR GO; GO:0048596; P:embryonic camera-type eye morphogenesis; IMP:BHF-UCL. DR GO; GO:0042733; P:embryonic digit morphogenesis; IMP:BHF-UCL. DR GO; GO:0035050; P:embryonic heart tube development; ISS:UniProtKB. DR GO; GO:0003272; P:endocardial cushion formation; ISO:MGI. DR GO; GO:0003203; P:endocardial cushion morphogenesis; IMP:BHF-UCL. DR GO; GO:0060441; P:epithelial tube branching involved in lung morphogenesis; IGI:UniProtKB. DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IMP:UniProtKB. DR GO; GO:0001947; P:heart looping; ISS:UniProtKB. DR GO; GO:0003007; P:heart morphogenesis; IMP:MGI. DR GO; GO:0060596; P:mammary placode formation; IGI:MGI. DR GO; GO:0097325; P:melanocyte proliferation; IMP:UniProtKB. DR GO; GO:0060916; P:mesenchymal cell proliferation involved in lung development; IMP:UniProtKB. DR GO; GO:0007521; P:muscle cell fate determination; IMP:MGI. DR GO; GO:1901211; P:negative regulation of cardiac chamber formation; IMP:BHF-UCL. DR GO; GO:2000773; P:negative regulation of cellular senescence; ISO:MGI. DR GO; GO:0045892; P:negative regulation of DNA-templated transcription; IDA:MGI. DR GO; GO:1901208; P:negative regulation of heart looping; IMP:BHF-UCL. DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB. DR GO; GO:0022008; P:neurogenesis; IMP:UniProtKB. DR GO; GO:0007219; P:Notch signaling pathway; IDA:MGI. DR GO; GO:0003151; P:outflow tract morphogenesis; IMP:UniProtKB. DR GO; GO:0003148; P:outflow tract septum morphogenesis; IMP:BHF-UCL. DR GO; GO:0060037; P:pharyngeal system development; IMP:BHF-UCL. DR GO; GO:0043474; P:pigment metabolic process involved in pigmentation; IMP:UniProtKB. DR GO; GO:0060045; P:positive regulation of cardiac muscle cell proliferation; IMP:BHF-UCL. DR GO; GO:1902808; P:positive regulation of cell cycle G1/S phase transition; IMP:UniProtKB. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB. DR GO; GO:0008016; P:regulation of heart contraction; ISS:UniProtKB. DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:BHF-UCL. DR GO; GO:0032526; P:response to retinoic acid; IDA:UniProtKB. DR GO; GO:0060021; P:roof of mouth development; IMP:MGI. DR GO; GO:0051145; P:smooth muscle cell differentiation; IGI:MGI. DR GO; GO:0072105; P:ureteric peristalsis; IGI:MGI. DR CDD; cd20188; T-box_TBX2_3-like; 1. DR Gene3D; 2.60.40.820; Transcription factor, T-box; 1. DR InterPro; IPR008967; p53-like_TF_DNA-bd_sf. DR InterPro; IPR046360; T-box_DNA-bd. DR InterPro; IPR036960; T-box_sf. DR InterPro; IPR022582; TBX2/3_TAD. DR InterPro; IPR048387; TBX2_3_RD. DR InterPro; IPR002070; TF_Brachyury. DR InterPro; IPR001699; TF_T-box. DR InterPro; IPR018186; TF_T-box_CS. DR PANTHER; PTHR11267; T-BOX PROTEIN-RELATED; 1. DR PANTHER; PTHR11267:SF82; T-BOX TRANSCRIPTION FACTOR TBX2; 1. DR Pfam; PF00907; T-box; 1. DR Pfam; PF12598; TBX; 1. DR Pfam; PF20627; TBX2-3_RD; 1. DR PRINTS; PR00938; BRACHYURY. DR PRINTS; PR00937; TBOX. DR SMART; SM00425; TBOX; 1. DR SUPFAM; SSF49417; p53-like transcription factors; 1. DR PROSITE; PS01283; TBOX_1; 1. DR PROSITE; PS01264; TBOX_2; 1. DR PROSITE; PS50252; TBOX_3; 1. DR Genevisible; Q60707; MM. PE 1: Evidence at protein level; KW Developmental protein; DNA-binding; Nucleus; Phosphoprotein; KW Reference proteome; Transcription; Transcription regulation. FT CHAIN 1..711 FT /note="T-box transcription factor TBX2" FT /id="PRO_0000184427" FT DNA_BIND 109..287 FT /note="T-box" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201" FT REGION 313..449 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 518..602 FT /note="Repression domain 1 (RD1)" FT /evidence="ECO:0000250" FT REGION 640..687 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 327..341 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 346..372 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 387..409 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 419..443 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 336 FT /note="Phosphoserine" FT /evidence="ECO:0000269|PubMed:18025091, FT ECO:0007744|PubMed:21183079" FT MOD_RES 342 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:21183079" FT MOD_RES 360 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:21183079" FT MOD_RES 623 FT /note="Phosphoserine" FT /evidence="ECO:0000269|PubMed:18025091" FT MOD_RES 652 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:Q13207" FT MOD_RES 656 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:Q13207" FT MOD_RES 675 FT /note="Phosphoserine" FT /evidence="ECO:0000269|PubMed:18025091" FT MUTAGEN 132..133 FT /note="RR->EE: Attenuated repression of Adam10 FT transcription." FT /evidence="ECO:0000269|PubMed:30599067" FT MUTAGEN 336 FT /note="S->A: Abolishes phosphorylation." FT /evidence="ECO:0000269|PubMed:18025091" FT MUTAGEN 623 FT /note="S->A: Abolishes phosphorylation." FT /evidence="ECO:0000269|PubMed:18025091" FT MUTAGEN 675 FT /note="S->A: Abolishes phosphorylation." FT /evidence="ECO:0000269|PubMed:18025091" FT CONFLICT 377 FT /note="G -> C (in Ref. 1; AAC52697 and 2; AAF90050)" FT /evidence="ECO:0000305" FT CONFLICT 486 FT /note="G -> C (in Ref. 1; AAC52697 and 2; AAF90050)" FT /evidence="ECO:0000305" FT CONFLICT 689 FT /note="S -> R (in Ref. 1; AAC52697 and 2; AAF90050)" FT /evidence="ECO:0000305" SQ SEQUENCE 711 AA; 75081 MW; 1C38BF1D25E1FF60 CRC64; MREPALAASA MAYHPFHAPR PADFPMSAFL AAAQPSFFPA LALPPGALGK PLPDPGLAGA AAAAAAAAAA AEAGLHVSAL GPHPPAAHLR SLKSLEPEDE VEDDPKVTLE AKELWDQFHK LGTEMVITKS GRRMFPPFKV RVSGLDKKAK YILLMDIVAA DDCRYKFHNS RWMVAGKADP EMPKRMYIHP DSPATGEQWM AKPVAFHKLK LTNNISDKHG FTILNSMHKY QPRFHIVRAN DILKLPYSTF RTYVFPETDF IAVTAYQNDK ITQLKIDNNP FAKGFRDTGN GRREKRKQLT LPTLRLYEEH CKPERDGAES DASSCDPPPA REPPPSPSAA PSPLRLHRAR AEEKPGAADS DPEPERTGEE RSAAPLGRSP SRDASPARLT EPERSRERRS PERCSKEPTE GGGDGPFSLR SLEKERPEAR RKDEGRKDVG EGKEPSLAPL VVQTDSASPL GAGHLPGLAF SSHLHGQQFF GPLGAGQPLF LHPGQFAMGP GAFSAMGMGH LLASVAGGSG SSGGAGPGTA AGLDAGGLGP AASAASTAAP FPFHLSQHML ASQGIPMPTF GGLFPYPYTY MAAAAAAASA LPATSAAAAA AAAAGSLSRS PFLGSARPRL RFSPYQIPVT IPPSTSLLTT GLAAEGSKGG NSREPSPLPE LALRKVGGPS RGALSPSGSA KEAASELQSI QRLVSGLESQ RALSPGRESP K //