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Q60707 (TBX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-box transcription factor TBX2

Short name=T-box protein 2
Gene names
Name:Tbx2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence. May be required for cardiac atrioventricular canal formation By similarity.

Subunit structure

Interacts with PML By similarity.

Subcellular location

Nucleus Potential.

Tissue specificity

In adults, highest levels in lung. Also found in heart, kidney, and ovary.

Developmental stage

Expression first observed at day 9.5 in the otic and optic vesicles and in the facial region. At day 12.5, expressed in the trigeminal ganglia, facial regions, retina and limb bud mesenchyme. In later stages, found in ear pinnae, the milk line, lung mesenchyme, body wall. Genital ridge and developing nervous system. Ref.1 Ref.6

Domain

The repression domain 1 (RD1) is necessary for its interaction with PML By similarity.

Sequence similarities

Contains 1 T-box DNA-binding domain.

Sequence caution

The sequence AAC52697.1 differs from that shown. Reason: Frameshift at position 4.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaorta morphogenesis

Inferred from mutant phenotype PubMed 15459098. Source: BHF-UCL

atrioventricular canal development

Inferred from mutant phenotype PubMed 15459098. Source: BHF-UCL

cardiac muscle tissue development

Inferred from mutant phenotype PubMed 15459098. Source: MGI

cell aging

Inferred from direct assay PubMed 11748239. Source: MGI

cellular senescence

Inferred from sequence or structural similarity. Source: UniProtKB

developmental growth involved in morphogenesis

Inferred from mutant phenotype PubMed 19769959. Source: MGI

embryonic camera-type eye morphogenesis

Inferred from mutant phenotype PubMed 15459098. Source: BHF-UCL

embryonic digit morphogenesis

Inferred from mutant phenotype PubMed 15459098. Source: BHF-UCL

endocardial cushion morphogenesis

Inferred from mutant phenotype PubMed 15459098. Source: BHF-UCL

heart morphogenesis

Inferred from mutant phenotype PubMed 15459098. Source: MGI

mammary placode formation

Inferred from genetic interaction PubMed 16222716. Source: MGI

muscle cell fate determination

Inferred from mutant phenotype PubMed 15459098. Source: MGI

negative regulation of cardiac chamber formation

Inferred from mutant phenotype PubMed 15042700. Source: BHF-UCL

negative regulation of heart looping

Inferred from mutant phenotype PubMed 15042700. Source: BHF-UCL

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 12023302PubMed 15042700. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 11748239PubMed 15843407. Source: MGI

outflow tract morphogenesis

Inferred from mutant phenotype PubMed 15042700PubMed 15459098. Source: BHF-UCL

outflow tract septum morphogenesis

Inferred from mutant phenotype PubMed 15459098. Source: BHF-UCL

palate development

Inferred from mutant phenotype PubMed 19769959. Source: MGI

pharynx development

Inferred from mutant phenotype PubMed 15459098. Source: BHF-UCL

positive regulation of cardiac muscle cell proliferation

Inferred from mutant phenotype PubMed 15042700. Source: BHF-UCL

regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation

Inferred from direct assay PubMed 15459098. Source: BHF-UCL

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 15042700. Source: MGI

transcription factor complex

Inferred from direct assay PubMed 12023302. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 15843407. Source: MGI

protein binding

Inferred from physical interaction PubMed 12023302. Source: MGI

sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 15843407. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711T-box transcription factor TBX2
PRO_0000184427

Regions

DNA binding109 – 287179T-box
Region518 – 60285Repression domain 1 (RD1) By similarity
Compositional bias28 – 8760Ala-rich
Compositional bias582 – 60423Ala-rich

Experimental info

Sequence conflict3771G → C in AAC52697. Ref.1
Sequence conflict3771G → C in AAF90050. Ref.2
Sequence conflict4861G → C in AAC52697. Ref.1
Sequence conflict4861G → C in AAF90050. Ref.2
Sequence conflict6891S → R in AAC52697. Ref.1
Sequence conflict6891S → R in AAF90050. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q60707 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 1C38BF1D25E1FF60

FASTA71175,081
        10         20         30         40         50         60 
MREPALAASA MAYHPFHAPR PADFPMSAFL AAAQPSFFPA LALPPGALGK PLPDPGLAGA 

        70         80         90        100        110        120 
AAAAAAAAAA AEAGLHVSAL GPHPPAAHLR SLKSLEPEDE VEDDPKVTLE AKELWDQFHK 

       130        140        150        160        170        180 
LGTEMVITKS GRRMFPPFKV RVSGLDKKAK YILLMDIVAA DDCRYKFHNS RWMVAGKADP 

       190        200        210        220        230        240 
EMPKRMYIHP DSPATGEQWM AKPVAFHKLK LTNNISDKHG FTILNSMHKY QPRFHIVRAN 

       250        260        270        280        290        300 
DILKLPYSTF RTYVFPETDF IAVTAYQNDK ITQLKIDNNP FAKGFRDTGN GRREKRKQLT 

       310        320        330        340        350        360 
LPTLRLYEEH CKPERDGAES DASSCDPPPA REPPPSPSAA PSPLRLHRAR AEEKPGAADS 

       370        380        390        400        410        420 
DPEPERTGEE RSAAPLGRSP SRDASPARLT EPERSRERRS PERCSKEPTE GGGDGPFSLR 

       430        440        450        460        470        480 
SLEKERPEAR RKDEGRKDVG EGKEPSLAPL VVQTDSASPL GAGHLPGLAF SSHLHGQQFF 

       490        500        510        520        530        540 
GPLGAGQPLF LHPGQFAMGP GAFSAMGMGH LLASVAGGSG SSGGAGPGTA AGLDAGGLGP 

       550        560        570        580        590        600 
AASAASTAAP FPFHLSQHML ASQGIPMPTF GGLFPYPYTY MAAAAAAASA LPATSAAAAA 

       610        620        630        640        650        660 
AAAAGSLSRS PFLGSARPRL RFSPYQIPVT IPPSTSLLTT GLAAEGSKGG NSREPSPLPE 

       670        680        690        700        710 
LALRKVGGPS RGALSPSGSA KEAASELQSI QRLVSGLESQ RALSPGRESP K 

« Hide

References

« Hide 'large scale' references
[1]"An ancient family of embryonically expressed mouse genes sharing a conserved protein motif with the T locus."
Bollag R.J., Siegfried Z., Cebra-Thomas J.A., Garvey N., Davison E.M., Silver L.M.
Nat. Genet. 7:383-389(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"The gene encoding the T-box Factor Tbx2 is a target for the microphthalmia-associated transcription factor in melanocytes."
Carreira S., Liu B., Goding C.R.
J. Biol. Chem. 275:21920-21927(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Expression of the T-box family genes, Tbx1-Tbx5, during early mouse development."
Chapman D.L., Garvey N., Hancock S., Alexiou M., Agulnik S.I., Gibson-Brown J.J., Cebra-Thomas J., Bollag R.J., Silver L.M., Papaioannou V.E.
Dev. Dyn. 206:379-390(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15566 mRNA. Translation: AAC52697.1. Frameshift.
AF244917 Genomic DNA. Translation: AAF90050.1.
AL596324, AL662910 Genomic DNA. Translation: CAI25161.1.
AL662910, AL596324 Genomic DNA. Translation: CAI25309.1.
CH466556 Genomic DNA. Translation: EDL15775.1.
BC156393 mRNA. No translation available.
CCDSCCDS36266.1.
PIRS46458.
RefSeqNP_033350.2. NM_009324.2.
UniGeneMm.287052.

3D structure databases

ProteinModelPortalQ60707.
SMRQ60707. Positions 102-287.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000000095.

PTM databases

PhosphoSiteQ60707.

Proteomic databases

PRIDEQ60707.

Protocols and materials databases

DNASU21385.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000095; ENSMUSP00000000095; ENSMUSG00000000093.
GeneID21385.
KEGGmmu:21385.
UCSCuc007ksb.1. mouse.

Organism-specific databases

CTD6909.
MGIMGI:98494. Tbx2.

Phylogenomic databases

eggNOGNOG240293.
GeneTreeENSGT00750000117325.
HOGENOMHOG000038046.
HOVERGENHBG000412.
InParanoidQ5SSP7.
KOK10176.
OMASPAPEHH.
OrthoDBEOG7W9RV1.
TreeFamTF106341.

Gene expression databases

ArrayExpressQ60707.
BgeeQ60707.
CleanExMM_TBX2.
GenevestigatorQ60707.

Family and domain databases

Gene3D2.60.40.820. 1 hit.
InterProIPR008967. p53-like_TF_DNA-bd.
IPR022582. TBX.
IPR002070. TF_Brachyury.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERPTHR11267. PTHR11267. 1 hit.
PfamPF00907. T-box. 1 hit.
PF12598. TBX. 1 hit.
[Graphical view]
PRINTSPR00938. BRACHYURY.
PR00937. TBOX.
SMARTSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMSSF49417. SSF49417. 1 hit.
PROSITEPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300620.
PROQ60707.
SOURCESearch...

Entry information

Entry nameTBX2_MOUSE
AccessionPrimary (citable) accession number: Q60707
Secondary accession number(s): Q5SSP7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot