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Protein

Enolase 1

Gene

eno1

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. Phosphoglycerate mutase (2,3-diphosphoglycerate-independent) (gpmA)
  4. Enolase 1 (eno1), Enolase 2 (eno2)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei156SubstrateUniRule annotation1
Binding sitei165SubstrateUniRule annotation1
Active sitei206Proton donorUniRule annotation1
Metal bindingi243MagnesiumUniRule annotation1
Metal bindingi287MagnesiumUniRule annotation1
Binding sitei287SubstrateUniRule annotation1
Metal bindingi314MagnesiumUniRule annotation1
Binding sitei314SubstrateUniRule annotation1
Active sitei339Proton acceptorUniRule annotation1
Binding sitei339Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei390SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMCAP243233:G1G0X-1914-MONOMER
UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 1UniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 1UniRule annotation
2-phosphoglycerate dehydratase 1UniRule annotation
Gene namesi
Name:eno1UniRule annotation
Synonyms:eno-1
Ordered Locus Names:MCA1933
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
Proteomesi
  • UP000006821 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001339191 – 421Enolase 1Add BLAST421

Proteomic databases

PRIDEiQ606T2

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi243233.MCA1933

Structurei

3D structure databases

ProteinModelPortaliQ606T2
SMRiQ606T2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni366 – 369Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA
HOGENOMiHOG000072174
KOiK01689
OMAiAAWTNFT
OrthoDBiPOG091H02DK

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q606T2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKARIESVQA MPIYDSRGVP TVRVRLTLTD GTAATASIPS GASTGENEAV
60 70 80 90 100
ELRDGDPKAH GGKGVLKAVS NVNDVIAPAV MGLEPWRQAE IDRLMIELDG
110 120 130 140 150
TANKERLGAN AILGVSEAVA AAAAKSAGLP LYAYLGGAGQ ARLPIPMINI
160 170 180 190 200
LNGGKHADSS LDFQEFMIMP VGAPSFAEAM RYATETFQAL KSILKAKGHA
210 220 230 240 250
TSVGDEGGFA PQLQSNDEAC DLIVEAITKA GYEPGEDIAI ALDPAASSFH
260 270 280 290 300
ENGVYRLTRS GQGDKTSSEM TDLYRRWIDK YPIVSIEDGL AENDWDGFRE
310 320 330 340 350
HTAVLGEKIQ IVGDDLLVTN TRFIQRAIEE KSCNAVLIKL NQIGTVTETV
360 370 380 390 400
EAIHLCRKAG WGFVISHRSG ETEDTFMADF AVAMGGGQIK TGSVCRSERM
410 420
AKYNRLLEIE SDLGKAARFG R
Length:421
Mass (Da):45,090
Last modified:November 23, 2004 - v1
Checksum:iE642D29FB0E31BA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA Translation: AAU92078.1
RefSeqiWP_010961180.1, NC_002977.6

Genome annotation databases

EnsemblBacteriaiAAU92078; AAU92078; MCA1933
KEGGimca:MCA1933

Similar proteinsi

Entry informationi

Entry nameiENO1_METCA
AccessioniPrimary (citable) accession number: Q606T2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: November 23, 2004
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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