Reviewed, UniProtKB/Swiss-Prot Q606N2 (NAGZ_METCA)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	MCA1984

Organism

Methylococcus capsulatus [Complete proteome] [HAMAP]

Taxonomic identifier

414 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Beta-hexosaminidase HAMAP MF_00364		PRO_0000234916
Sites
	Active site	242	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q606N2-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 38423296CB06F04D	FASTA	334	35,944
	10 20 30 40 50 60 MFDLVGPRLS ADEREFLCHP AAGGLILFSR NYASPDQMLA LVSEVRSLRP DMLIAVDHEG 70 80 90 100 110 120 GRVQRFREGF TRLPPASAYL EVAGEAGLAA AETAGWLMAA ELRAVGVDFS FAPVLDVDSG 130 140 150 160 170 180 ISTVIGDRAF ARTPEEVTAA ARAFATGMRR AGMAAVGKHF PGHGGVAGDS HLVLPEDRRE 190 200 210 220 230 240 LEELLARDLL PFSALIRENL EGIMPAHVLY SRIDARPPCF SPFWLQTILR ERMNFDGAIF 250 260 270 280 290 300 SDDLSMAGAA VAGDYAARAL AALEAGCDML VVCNTPEATA SILEALENRT ASPGSTRRLA 310 320 330 AMCGRSRIDR DALLASSEWR NAVDRIHSFN DSAQ

References

[1]

"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)." Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. , Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A. PLoS Biol. 2:1616-1628(2004) [PubMed: 15383840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bath / NCIMB 11132.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017282 Genomic DNA. Translation: AAU91985.1.
RefSeq	YP_114416.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3103253.
GenomeReviews	Gene locus MCA1984 in contig AE017282_GR.
KEGG	mca:MCA1984.
NMPDR	fig|243233.4.peg.1935.
TIGR	MCA1984.
Phylogenomic databases
HOGENOM	Q606N2.
OMA	ADSHIAQ.
Enzyme and pathway databases
BioCyc	MCAP243233:MCA_1984-MON.
BRENDA	3.2.1.52. 2172.
Family and domain databases
HAMAP	MF_00364. [Tree]
InterPro	IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_METCA

Accession

Primary (citable) accession number: Q606N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)