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Q60698 (SKI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ski oncogene
Alternative name(s):
Proto-oncogene c-Ski
Gene names
Name:Ski
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length725 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.

Subunit structure

Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes By similarity.

Subcellular location

Nucleus.

Developmental stage

Is expressed in a uniform pattern in all embryonic cells prior to skeletal muscle cell formation in the myotomes of somites. Expression is first up-regulated in skeletal muscle at 12 dpc, this up-regulation is evident first in body wall muscle and one day later in limb muscles. At 13.5 dpc a most prominent expression is seen in all skeletal muscles. At this stage expression is seen in all other cells and tissues but at lower levels than in skeletal muscle.

Sequence similarities

Belongs to the SKI family.

Ontologies

Keywords
   Cellular componentNucleus
   DiseaseProto-oncogene
   DomainCoiled coil
Repeat
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSMAD protein signal transduction

Inferred from sequence or structural similarity. Source: BHF-UCL

anterior/posterior axis specification

Inferred from mutant phenotype PubMed 11731796. Source: MGI

bone morphogenesis

Inferred from mutant phenotype PubMed 9284043. Source: MGI

camera-type eye development

Inferred from mutant phenotype PubMed 11731796. Source: MGI

camera-type eye morphogenesis

Inferred from mutant phenotype PubMed 17003410. Source: MGI

embryonic limb morphogenesis

Inferred from mutant phenotype PubMed 12435627. Source: MGI

face morphogenesis

Inferred from mutant phenotype PubMed 11731796PubMed 9284043. Source: MGI

lens morphogenesis in camera-type eye

Inferred from mutant phenotype PubMed 17003410. Source: MGI

myelination in peripheral nervous system

Inferred from mutant phenotype PubMed 15312649. Source: MGI

myotube differentiation

Inferred from direct assay PubMed 17352741. Source: MGI

negative regulation of BMP signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of Schwann cell proliferation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of activin receptor signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of cell proliferation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of fibroblast proliferation

Inferred from mutant phenotype PubMed 11781823. Source: MGI

negative regulation of histone deacetylation

Inferred from genetic interaction PubMed 17352741. Source: GOC

negative regulation of osteoblast differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 10049357. Source: MGI

negative regulation of transforming growth factor beta receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

neural tube closure

Inferred from mutant phenotype PubMed 9284043. Source: MGI

nose morphogenesis

Inferred from mutant phenotype PubMed 11731796. Source: MGI

olfactory bulb development

Inferred from mutant phenotype PubMed 11731796. Source: MGI

palate development

Inferred from mutant phenotype PubMed 11731796. Source: MGI

positive regulation of DNA binding

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 17352741PubMed 7659522. Source: MGI

protein heterotrimerization

Inferred from sequence or structural similarity. Source: BHF-UCL

protein homotrimerization

Inferred from sequence or structural similarity. Source: UniProtKB

retina development in camera-type eye

Inferred from mutant phenotype PubMed 17003410. Source: MGI

skeletal muscle fiber development

Inferred from mutant phenotype PubMed 9284043. Source: MGI

somatic stem cell maintenance

Inferred from direct assay PubMed 19379700. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

   Cellular_componentPML body

Inferred from sequence or structural similarity. Source: BHF-UCL

cytoplasm

Inferred from direct assay PubMed 12435627. Source: MGI

nuclear body

Inferred from sequence or structural similarity. Source: BHF-UCL

nucleoplasm

Inferred from sequence or structural similarity. Source: BHF-UCL

nucleus

Inferred from direct assay PubMed 10049357PubMed 12435627PubMed 17352741PubMed 7823166. Source: MGI

protein complex

Inferred from sequence orthology PubMed 23610558. Source: MGI

transcription factor complex

Inferred from physical interaction PubMed 19008232. Source: MGI

transcriptional repressor complex

Inferred from direct assay PubMed 12435627. Source: MGI

   Molecular_functionSMAD binding

Inferred from sequence or structural similarity. Source: BHF-UCL

chromatin binding

Inferred from genetic interaction PubMed 19008232. Source: MGI

histone deacetylase inhibitor activity

Inferred from genetic interaction PubMed 17352741. Source: MGI

protein domain specific binding

Inferred from sequence or structural similarity. Source: BHF-UCL

protein kinase binding

Inferred from sequence or structural similarity. Source: BHF-UCL

repressing transcription factor binding

Inferred from sequence or structural similarity. Source: BHF-UCL

transcription corepressor activity

Inferred from direct assay PubMed 10049357. Source: MGI

ubiquitin protein ligase binding

Inferred from sequence or structural similarity. Source: BHF-UCL

zinc ion binding

Inferred from sequence or structural similarity. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 725725Ski oncogene
PRO_0000129383

Regions

Coiled coil534 – 708175 Potential

Amino acid modifications

Modified residue4771Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60698 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: 1BFD05C38519505C

FASTA72580,119
        10         20         30         40         50         60 
MEAAAAGRGG FQQPGLQKTL EQFHLSSMSS LGGPAVSRRA GQEAYKKESA KEAGAATVPA 

        70         80         90        100        110        120 
PVPTAAEPPP VLHLPAIQPP PPVLPGPFFM PSDRSTERCE TVLEGETISC FVVGGEKRLC 

       130        140        150        160        170        180 
LPQILNSVLR DFSLQQINSV CDELHIYCSR CTADQLEILK VMGILPFSAP SCGLITKTDA 

       190        200        210        220        230        240 
ERLCNALLYG GAYPPPCKKE LAASLALGLE LSERSVRVYH ECFGKCKGLL VPELYSSPSA 

       250        260        270        280        290        300 
ACIQCLDCRL MYPPHKFVVH SHKALENRTC HWGFDSANWR AYILLSQDYT GKEEQARLGR 

       310        320        330        340        350        360 
CLDDVKEKFD YANKYKRRVP RVSEPPASIR PKTDDTSSQS PASSEKDKQS TWLRTLAGSS 

       370        380        390        400        410        420 
NKSLGCTHPR QRLSAFRPWS PAVSASEKET SPHLPALIRD SFYSYKSFET AVAPNVALAP 

       430        440        450        460        470        480 
PTQQKVVNSP PCTTVVSRAP EPLTTCIQPR KRKLTLDTAG APDMLTPVAA AEEDKDSEAE 

       490        500        510        520        530        540 
VEVESREEFT SSLSSLSSPS FTSSSSAKDL SSPGMHAPPV VAPDAAAHVD APSGLEAELE 

       550        560        570        580        590        600 
HLRQALEGGL DTKEAKEKFL HEVVKMRVKQ EEKLTAALQA KRTLHQELEF LRVAKKEKLR 

       610        620        630        640        650        660 
EATEAKRNLR KEIERLRAEN EKKMKEANES RVRLKRELEQ ARQVRVCDKG CEAGRLRAKY 

       670        680        690        700        710        720 
SAQVEDLQAK LQHAEADREQ LRADLLRERE AREHLEKVVR ELQEQLRPRP RPEHPGGESN 


AELGP 

« Hide

References

[1]"Mouse Ski proto-oncogene cDNA."
Chen Y., Berk M., Chen H., Stavnezer E., Colmenares C.
Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv.
[2]"Enhanced expression of mouse c-ski accompanies terminal skeletal muscle differentiation in vivo and in vitro."
Namciu S., Lyons G.E., Micales B.K., Heyman H.-C., Colmenares C., Stavnezer E.
Dev. Dyn. 204:291-300(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-324.
Strain: 129/J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF435852 mRNA. Translation: AAL30825.1.
U14173 Genomic DNA. Translation: AAA99669.1. Sequence problems.
UniGeneMm.28520.

3D structure databases

ProteinModelPortalQ60698.
SMRQ60698. Positions 89-190, 216-310.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60020N.

PTM databases

PhosphoSiteQ60698.

Proteomic databases

PRIDEQ60698.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:98310. Ski.

Phylogenomic databases

eggNOGNOG82850.
HOVERGENHBG006599.
InParanoidQ60698.

Gene expression databases

CleanExMM_SKI.
GenevestigatorQ60698.

Family and domain databases

Gene3D3.10.260.20. 1 hit.
3.10.390.10. 1 hit.
InterProIPR014890. c-SKI_SMAD4-bd_dom.
IPR009061. DNA-bd_dom_put.
IPR010919. SAND_dom-like.
IPR028760. Ski.
IPR003380. Transform_Ski.
IPR023216. Tscrpt_reg_SKI_SnoN.
[Graphical view]
PANTHERPTHR10005. PTHR10005. 1 hit.
PTHR10005:SF2. PTHR10005:SF2. 1 hit.
PfamPF08782. c-SKI_SMAD_bind. 1 hit.
PF02437. Ski_Sno. 1 hit.
[Graphical view]
SMARTSM01046. c-SKI_SMAD_bind. 1 hit.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF63763. SSF63763. 1 hit.
ProtoNetSearch...

Other

PROQ60698.
SOURCESearch...

Entry information

Entry nameSKI_MOUSE
AccessionPrimary (citable) accession number: Q60698
Secondary accession number(s): Q8VIL5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 21, 2005
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot