Q60698 (SKI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ski oncogene

Alternative name(s):
Proto-oncogene c-Ski

Gene names

Name:

Ski

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Subunit structure	Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes By similarity.
Subcellular location	Nucleus.
Developmental stage	Is expressed in a uniform pattern in all embryonic cells prior to skeletal muscle cell formation in the myotomes of somites. Expression is first up-regulated in skeletal muscle at 12 dpc, this up-regulation is evident first in body wall muscle and one day later in limb muscles. At 13.5 dpc a most prominent expression is seen in all skeletal muscles. At this stage expression is seen in all other cells and tissues but at lower levels than in skeletal muscle.
Sequence similarities	Belongs to the SKI family.

Ontologies

Keywords
Cellular component	Nucleus
Disease	Proto-oncogene
Domain	Coiled coil Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SMAD protein signal transduction Inferred from sequence or structural similarity. Source: BHF-UCL anterior/posterior axis specification Inferred from mutant phenotype PubMed 11731796. Source: MGI bone morphogenesis Inferred from mutant phenotype PubMed 9284043. Source: MGI embryonic limb morphogenesis Inferred from mutant phenotype PubMed 12435627. Source: MGI face morphogenesis Inferred from mutant phenotype PubMed 11731796PubMed 9284043. Source: MGI lens morphogenesis in camera-type eye Inferred from mutant phenotype PubMed 17003410. Source: MGI myelination in peripheral nervous system Inferred from mutant phenotype PubMed 15312649. Source: MGI myotube differentiation Inferred from direct assay PubMed 17352741. Source: MGI negative regulation of BMP signaling pathway Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of Schwann cell proliferation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of activin receptor signaling pathway Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of fibroblast proliferation Inferred from mutant phenotype PubMed 11781823. Source: MGI negative regulation of osteoblast differentiation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 10049357. Source: MGI negative regulation of transforming growth factor beta receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB neural tube closure Inferred from mutant phenotype PubMed 9284043. Source: MGI nose morphogenesis Inferred from mutant phenotype PubMed 11731796. Source: MGI olfactory bulb development Inferred from mutant phenotype PubMed 11731796. Source: MGI palate development Inferred from mutant phenotype PubMed 11731796. Source: MGI positive regulation of DNA binding Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 17352741PubMed 7659522. Source: MGI protein heterotrimerization Inferred from sequence or structural similarity. Source: BHF-UCL protein homotrimerization Inferred from sequence or structural similarity. Source: UniProtKB regulation of apoptotic process Inferred from mutant phenotype PubMed 9284043. Source: MGI retina development in camera-type eye Inferred from mutant phenotype PubMed 17003410. Source: MGI skeletal muscle fiber development Inferred from mutant phenotype PubMed 9284043. Source: MGI somatic stem cell maintenance Inferred from direct assay PubMed 19379700. Source: MGI
Cellular_component	PML body Inferred from sequence or structural similarity. Source: BHF-UCL cytoplasm Inferred from direct assay PubMed 12435627. Source: MGI transcription factor complex Inferred from physical interaction PubMed 19008232. Source: MGI transcriptional repressor complex Inferred from direct assay PubMed 12435627. Source: MGI
Molecular_function	SMAD binding Inferred from sequence or structural similarity. Source: BHF-UCL chromatin binding Inferred from genetic interaction PubMed 19008232. Source: MGI histone deacetylase inhibitor activity Inferred from genetic interaction PubMed 17352741. Source: MGI nucleotide binding Inferred from electronic annotation. Source: InterPro protein domain specific binding Inferred from sequence or structural similarity. Source: BHF-UCL protein kinase binding Inferred from sequence or structural similarity. Source: BHF-UCL repressing transcription factor binding Inferred from sequence or structural similarity. Source: BHF-UCL transcription corepressor activity Inferred from direct assay PubMed 10049357. Source: MGI ubiquitin protein ligase binding Inferred from sequence or structural similarity. Source: BHF-UCL zinc ion binding Inferred from sequence or structural similarity. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Ski oncogene		PRO_0000129383
Regions
	Coiled coil	534 – 708	175	Potential
Amino acid modifications
	Modified residue	477	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60698 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 1BFD05C38519505C	FASTA	725	80,119
	10 20 30 40 50 60 MEAAAAGRGG FQQPGLQKTL EQFHLSSMSS LGGPAVSRRA GQEAYKKESA KEAGAATVPA 70 80 90 100 110 120 PVPTAAEPPP VLHLPAIQPP PPVLPGPFFM PSDRSTERCE TVLEGETISC FVVGGEKRLC 130 140 150 160 170 180 LPQILNSVLR DFSLQQINSV CDELHIYCSR CTADQLEILK VMGILPFSAP SCGLITKTDA 190 200 210 220 230 240 ERLCNALLYG GAYPPPCKKE LAASLALGLE LSERSVRVYH ECFGKCKGLL VPELYSSPSA 250 260 270 280 290 300 ACIQCLDCRL MYPPHKFVVH SHKALENRTC HWGFDSANWR AYILLSQDYT GKEEQARLGR 310 320 330 340 350 360 CLDDVKEKFD YANKYKRRVP RVSEPPASIR PKTDDTSSQS PASSEKDKQS TWLRTLAGSS 370 380 390 400 410 420 NKSLGCTHPR QRLSAFRPWS PAVSASEKET SPHLPALIRD SFYSYKSFET AVAPNVALAP 430 440 450 460 470 480 PTQQKVVNSP PCTTVVSRAP EPLTTCIQPR KRKLTLDTAG APDMLTPVAA AEEDKDSEAE 490 500 510 520 530 540 VEVESREEFT SSLSSLSSPS FTSSSSAKDL SSPGMHAPPV VAPDAAAHVD APSGLEAELE 550 560 570 580 590 600 HLRQALEGGL DTKEAKEKFL HEVVKMRVKQ EEKLTAALQA KRTLHQELEF LRVAKKEKLR 610 620 630 640 650 660 EATEAKRNLR KEIERLRAEN EKKMKEANES RVRLKRELEQ ARQVRVCDKG CEAGRLRAKY 670 680 690 700 710 720 SAQVEDLQAK LQHAEADREQ LRADLLRERE AREHLEKVVR ELQEQLRPRP RPEHPGGESN AELGP

References

[1]	"Mouse Ski proto-oncogene cDNA." Chen Y., Berk M., Chen H., Stavnezer E., Colmenares C. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/Sv.
[2]	"Enhanced expression of mouse c-ski accompanies terminal skeletal muscle differentiation in vivo and in vitro." Namciu S., Lyons G.E., Micales B.K., Heyman H.-C., Colmenares C., Stavnezer E. Dev. Dyn. 204:291-300(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-324. Strain: 129/J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF435852 mRNA. Translation: AAL30825.1. U14173 Genomic DNA. Translation: AAA99669.1. Sequence problems.
IPI	IPI00987407.
UniGene	Mm.28520.
3D structure databases
ProteinModelPortal	Q60698.
SMR	Q60698. Positions 89-190, 216-310.
ModBase	Search...
PTM databases
PhosphoSite	Q60698.
Proteomic databases
PRIDE	Q60698.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:98310. Ski.
Phylogenomic databases
eggNOG	NOG82850.
HOVERGEN	HBG006599.
InParanoid	Q60698.
OrthoDB	EOG44QT1K.
Gene expression databases
CleanEx	MM_SKI.
Genevestigator	Q60698.
GermOnline	ENSMUSG00000029050. Mus musculus.
Family and domain databases
Gene3D	3.10.260.20. 1 hit. 3.10.390.10. 1 hit.
InterPro	IPR014890. c-SKI_SMAD4-bd_dom. IPR009061. DNA-bd_dom_put. IPR010919. SAND_dom-like. IPR003380. Transform_Ski. IPR023216. Tscrpt_reg_SKI_SnoN. [Graphical view]
PANTHER	PTHR10005. PTHR10005. 1 hit.
Pfam	PF08782. c-SKI_SMAD_bind. 1 hit. PF02437. Ski_Sno. 1 hit. [Graphical view]
SMART	SM01046. c-SKI_SMAD_bind. 1 hit. [Graphical view]
SUPFAM	SSF46955. Putativ_DNA_bind. 1 hit. SSF63763. SAND_like. 1 hit.
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

SKI_MOUSE

Accession

Primary (citable) accession number: Q60698
Secondary accession number(s): Q8VIL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 21, 2005
Last modified:	April 3, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program