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Protein

A-kinase anchor protein 4

Gene

Akap4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major structural component of sperm fibrous sheath. Plays a role in sperm motility (PubMed:12167408).1 Publication

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB

GO - Biological processi

  • establishment of protein localization Source: MGI
  • motile cilium assembly Source: MGI
  • protein localization Source: MGI
  • sperm motility Source: UniProtKB
  • transmembrane receptor protein serine/threonine kinase signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 4
Short name:
AKAP-4
Alternative name(s):
A-kinase anchor protein 82 kDa
Short name:
AKAP 82
Short name:
mAKAP82
FSC1
Major sperm fibrous sheath protein
Protein kinase A-anchoring protein 4
Short name:
PRKA4
p82
Gene namesi
Name:Akap4
Synonyms:Akap82, Fsc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:102794. Akap4.

Subcellular locationi

  • Cell projectionciliumflagellum By similarity

  • Note: Localizes to the principle piece of the sperm flagellum.By similarity

GO - Cellular componenti

  • cilium Source: MGI
  • motile cilium Source: UniProtKB
  • nucleus Source: MGI
  • perinuclear region of cytoplasm Source: UniProtKB
  • sperm fibrous sheath Source: MGI
  • sperm principal piece Source: MGI
  • Z disc Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Flagellum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 188188Sequence analysisPRO_0000020659Add
BLAST
Chaini189 – 849661A-kinase anchor protein 4PRO_0000020660Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei96 – 961PhosphoserineBy similarity
Modified residuei130 – 1301PhosphoserineBy similarity
Modified residuei190 – 1901PhosphoserineBy similarity
Modified residuei204 – 2041PhosphoserineBy similarity
Modified residuei207 – 2071PhosphothreonineBy similarity
Modified residuei213 – 2131PhosphoserineBy similarity
Modified residuei226 – 2261PhosphoserineBy similarity
Modified residuei271 – 2711PhosphoserineBy similarity
Modified residuei301 – 3011PhosphotyrosineBy similarity
Modified residuei302 – 3021PhosphoserineBy similarity
Modified residuei341 – 3411PhosphoserineBy similarity
Modified residuei431 – 4311PhosphoserineBy similarity
Modified residuei442 – 4421PhosphoserineBy similarity
Modified residuei444 – 4441PhosphoserineBy similarity
Modified residuei463 – 4631PhosphoserineBy similarity
Modified residuei492 – 4921PhosphoserineBy similarity
Modified residuei497 – 4971PhosphoserineBy similarity
Modified residuei504 – 5041PhosphoserineBy similarity
Modified residuei506 – 5061PhosphothreonineBy similarity
Modified residuei538 – 5381PhosphoserineBy similarity
Modified residuei628 – 6281PhosphoserineBy similarity
Modified residuei633 – 6331PhosphoserineBy similarity
Modified residuei652 – 6521PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60662.
PRIDEiQ60662.

PTM databases

iPTMnetiQ60662.
PhosphoSiteiQ60662.

Expressioni

Tissue specificityi

Spermatid.

Developmental stagei

Post-meiotic phase of spermatogenesis.

Gene expression databases

BgeeiQ60662.
CleanExiMM_AKAP4.
ExpressionAtlasiQ60662. baseline and differential.
GenevisibleiQ60662. MM.

Interactioni

Subunit structurei

Interacts with PRKAR1A and PRKAR2A (PubMed:9852104). Interacts with ENO4 (PubMed:23446454).2 Publications

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050962.

Structurei

3D structure databases

ProteinModelPortaliQ60662.
SMRiQ60662. Positions 766-847.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni219 – 23214PKA-RI and PKA-RII subunit binding domainAdd
BLAST
Regioni335 – 34410PKA-RI-alpha subunit binding domain

Domaini

RI-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.1 Publication

Sequence similaritiesi

Belongs to the AKAP110 family.Curated

Phylogenomic databases

eggNOGiENOG410IKID. Eukaryota.
ENOG411157C. LUCA.
GeneTreeiENSGT00420000029845.
HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiQ60662.
KOiK16521.
OMAiKCLHHSI.
OrthoDBiEOG7W41B2.
PhylomeDBiQ60662.
TreeFamiTF105403.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 3 hits.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60662-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIAYCGTTTM SDDIDWLHSR RGVCKVDLYS PKGQQDQDRK VICFVDVSTL
60 70 80 90 100
NVEDKDSKGA AGSRSEGELN LETLEEKEII VIKDTEKQDQ SKTEGSVCLF
110 120 130 140 150
KQAPSDPISV LNWLLNDLQK YALGFQHALS PSASSCKHKV GDLEGDYSKI
160 170 180 190 200
PSENCYSVYA DQVNFDYLNK GPQNLRLEMA ASKNTNNNQS PSNPATKSPS
210 220 230 240 250
NQRSVATPEG ECSMDDLSFY VNRLSSLVIQ MARKEIKDKL EGGSKCLHHS
260 270 280 290 300
MYTSGDKGKT SPRSAVSKIA SEMAHEAVEL TSSEMRGNGE DCRDGRKTFL
310 320 330 340 350
YSEMCNKNKC GEKQQMCPKD SKEFADSISK GLMVYANQVA SDMMVSVMKT
360 370 380 390 400
LKVHSCGKPI PACVVLKRVL LKHTKEIVSD LIDSCMKNLH NITGVLMTDS
410 420 430 440 450
DFVSAVKRNL FNHGKQNAAD IMEAMLKRLV SALLGEKKET KSQSLAYATL
460 470 480 490 500
KAGTNDPKCK NQSLEFSAMK AEMKGKDKCK SKADPCCKSL TSAERVSEHI
510 520 530 540 550
LKESLTMWNN QKQGNQGKVT NKVCCTSKDE KREKISPSTD SLAKDLIVSA
560 570 580 590 600
LMLIQYHLTQ QAKGKDPCEE ECPGSSMGYM SQSAQYEKCG GGQSSKSLSM
610 620 630 640 650
KHFETRGAPG PSTCMKENQL ESQKMDMSNM VLSLIQKLLS ESPFSCDELT
660 670 680 690 700
ESDNKRCCDP RSSKAAPMAK RPEEQCQDNA ELDFISGMKQ MNRQFIDQLV
710 720 730 740 750
ESVMKLCLIM AKYSNNGAAL AELEEQAALV GSGSRCGRDA MMSQNYSETP
760 770 780 790 800
GPEVIVNNQC STTNLQKQLQ AVLQWIAASQ FNVPMLYFMG DDDGQLEKLP
810 820 830 840
EVSAKAAEKG YSVGDLLQEV MKFAKERQLD EAVGNMARKQ LLDWLLANL
Length:849
Mass (Da):93,796
Last modified:November 1, 1996 - v1
Checksum:i8DAC8939CF1CB81A
GO
Isoform 2 (identifier: Q60662-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Show »
Length:840
Mass (Da):92,854
Checksum:i38C1D7AFA330A198
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti341 – 3411S → E AA sequence (PubMed:7711182).Curated
Sequence conflicti444 – 4441S → D AA sequence (PubMed:7711182).Curated
Sequence conflicti445 – 4451L → T AA sequence (PubMed:7711182).Curated
Sequence conflicti449 – 4491T → P in AAA53216 (PubMed:8088444).Curated
Sequence conflicti480 – 4801K → T in AAA53216 (PubMed:8088444).Curated
Sequence conflicti510 – 5101N → T in AAA53216 (PubMed:8088444).Curated
Sequence conflicti547 – 5471I → T AA sequence (PubMed:7711182).Curated
Sequence conflicti549 – 5491S → P AA sequence (PubMed:7711182).Curated
Sequence conflicti564 – 5641G → A in AAA53216 (PubMed:8088444).Curated
Sequence conflicti714 – 7141S → R in AAA53216 (PubMed:8088444).Curated
Sequence conflicti721 – 7211A → G in AAA53216 (PubMed:8088444).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 99Missing in isoform 2. CuratedVSP_004100

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10341 mRNA. Translation: AAA75597.1.
U07423 mRNA. Translation: AAA53216.1.
AF448786, AF448784, AF448785 Genomic DNA. Translation: AAM18539.1.
AF448786, AF448785 Genomic DNA. Translation: AAM18540.1.
AL808124 Genomic DNA. Translation: CAM15283.1.
CCDSiCCDS40838.1. [Q60662-1]
CCDS40839.1. [Q60662-2]
PIRiI49060.
RefSeqiNP_001036007.1. NM_001042542.2. [Q60662-2]
NP_033781.2. NM_009651.4. [Q60662-1]
UniGeneiMm.1498.

Genome annotation databases

EnsembliENSMUST00000057101; ENSMUSP00000050962; ENSMUSG00000050089. [Q60662-1]
ENSMUST00000115750; ENSMUSP00000111416; ENSMUSG00000050089. [Q60662-2]
ENSMUST00000115751; ENSMUSP00000111417; ENSMUSG00000050089. [Q60662-2]
GeneIDi11643.
KEGGimmu:11643.
UCSCiuc009slc.2. mouse. [Q60662-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10341 mRNA. Translation: AAA75597.1.
U07423 mRNA. Translation: AAA53216.1.
AF448786, AF448784, AF448785 Genomic DNA. Translation: AAM18539.1.
AF448786, AF448785 Genomic DNA. Translation: AAM18540.1.
AL808124 Genomic DNA. Translation: CAM15283.1.
CCDSiCCDS40838.1. [Q60662-1]
CCDS40839.1. [Q60662-2]
PIRiI49060.
RefSeqiNP_001036007.1. NM_001042542.2. [Q60662-2]
NP_033781.2. NM_009651.4. [Q60662-1]
UniGeneiMm.1498.

3D structure databases

ProteinModelPortaliQ60662.
SMRiQ60662. Positions 766-847.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050962.

PTM databases

iPTMnetiQ60662.
PhosphoSiteiQ60662.

Proteomic databases

PaxDbiQ60662.
PRIDEiQ60662.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057101; ENSMUSP00000050962; ENSMUSG00000050089. [Q60662-1]
ENSMUST00000115750; ENSMUSP00000111416; ENSMUSG00000050089. [Q60662-2]
ENSMUST00000115751; ENSMUSP00000111417; ENSMUSG00000050089. [Q60662-2]
GeneIDi11643.
KEGGimmu:11643.
UCSCiuc009slc.2. mouse. [Q60662-1]

Organism-specific databases

CTDi8852.
MGIiMGI:102794. Akap4.

Phylogenomic databases

eggNOGiENOG410IKID. Eukaryota.
ENOG411157C. LUCA.
GeneTreeiENSGT00420000029845.
HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiQ60662.
KOiK16521.
OMAiKCLHHSI.
OrthoDBiEOG7W41B2.
PhylomeDBiQ60662.
TreeFamiTF105403.

Miscellaneous databases

ChiTaRSiAkap4. mouse.
NextBioi279237.
PROiQ60662.
SOURCEiSearch...

Gene expression databases

BgeeiQ60662.
CleanExiMM_AKAP4.
ExpressionAtlasiQ60662. baseline and differential.
GenevisibleiQ60662. MM.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 3 hits.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The major fibrous sheath polypeptide of mouse sperm: structural and functional similarities to the A-kinase anchoring proteins."
    Carrera A., Gerton G.L., Moss S.B.
    Dev. Biol. 165:272-284(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
    Strain: CD-1.
    Tissue: Sperm.
  2. "Characterization of Fsc1 cDNA for a mouse sperm fibrous sheath component."
    Fulcher K.D., Mori C., Welch J.E., O'Brien D.A., Klapper D.G., Eddy E.M.
    Biol. Reprod. 52:41-49(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
    Strain: CD-1.
    Tissue: Spermatid.
  3. "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum."
    Brown P.R., Miki K., Harper D.B., Eddy E.M.
    Biol. Reprod. 68:2241-2248(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
    Strain: 129/Sv.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "Identification of tethering domains for protein kinase A type Ialpha regulatory subunits on sperm fibrous sheath protein FSC1."
    Miki K., Eddy E.M.
    J. Biol. Chem. 273:34384-34390(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRKAR1A AND PRKAR2A, DOMAIN.
  6. "Targeted disruption of the Akap4 gene causes defects in sperm flagellum and motility."
    Miki K., Willis W.D., Brown P.R., Goulding E.H., Fulcher K.D., Eddy E.M.
    Dev. Biol. 248:331-342(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  8. "Disruption of a spermatogenic cell-specific mouse enolase 4 (eno4) gene causes sperm structural defects and male infertility."
    Nakamura N., Dai Q., Williams J., Goulding E.H., Willis W.D., Brown P.R., Eddy E.M.
    Biol. Reprod. 88:90-90(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ENO4.

Entry informationi

Entry nameiAKAP4_MOUSE
AccessioniPrimary (citable) accession number: Q60662
Secondary accession number(s): B1AXM7, Q60630, Q8R2G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.