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Protein

A-kinase anchor protein 4

Gene

Akap4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major structural component of sperm fibrous sheath. Plays a role in sperm motility (PubMed:12167408).1 Publication

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB

GO - Biological processi

  • establishment of protein localization Source: MGI
  • flagellated sperm motility Source: UniProtKB
  • motile cilium assembly Source: MGI
  • protein localization Source: MGI
  • regulation of protein kinase A signaling Source: GO_Central
  • transmembrane receptor protein serine/threonine kinase signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 4
Short name:
AKAP-4
Alternative name(s):
A-kinase anchor protein 82 kDa
Short name:
AKAP 82
Short name:
mAKAP82
FSC1
Major sperm fibrous sheath protein
Protein kinase A-anchoring protein 4
Short name:
PRKA4
p82
Gene namesi
Name:Akap4
Synonyms:Akap82, Fsc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:102794. Akap4.

Subcellular locationi

  • Cell projectionciliumflagellum By similarity

  • Note: Localizes to the principle piece of the sperm flagellum.By similarity

GO - Cellular componenti

  • cilium Source: MGI
  • motile cilium Source: UniProtKB
  • nucleus Source: MGI
  • perinuclear region of cytoplasm Source: UniProtKB
  • sperm fibrous sheath Source: MGI
  • sperm principal piece Source: MGI
  • Z disc Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Flagellum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000206591 – 188Sequence analysisAdd BLAST188
ChainiPRO_0000020660189 – 849A-kinase anchor protein 4Add BLAST661

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96PhosphoserineBy similarity1
Modified residuei130PhosphoserineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei204PhosphoserineBy similarity1
Modified residuei207PhosphothreonineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei226PhosphoserineBy similarity1
Modified residuei271PhosphoserineBy similarity1
Modified residuei301PhosphotyrosineBy similarity1
Modified residuei302PhosphoserineBy similarity1
Modified residuei341PhosphoserineBy similarity1
Modified residuei431PhosphoserineBy similarity1
Modified residuei442PhosphoserineBy similarity1
Modified residuei444PhosphoserineBy similarity1
Modified residuei463PhosphoserineBy similarity1
Modified residuei492PhosphoserineBy similarity1
Modified residuei497PhosphoserineBy similarity1
Modified residuei504PhosphoserineBy similarity1
Modified residuei506PhosphothreonineBy similarity1
Modified residuei538PhosphoserineBy similarity1
Modified residuei628PhosphoserineBy similarity1
Modified residuei633PhosphoserineBy similarity1
Modified residuei652PhosphoserineBy similarity1
Modified residuei702PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60662.
PRIDEiQ60662.

PTM databases

iPTMnetiQ60662.
PhosphoSitePlusiQ60662.

Expressioni

Tissue specificityi

Spermatid.

Developmental stagei

Post-meiotic phase of spermatogenesis.

Gene expression databases

BgeeiENSMUSG00000050089.
CleanExiMM_AKAP4.
ExpressionAtlasiQ60662. baseline and differential.
GenevisibleiQ60662. MM.

Interactioni

Subunit structurei

Interacts with PRKAR1A and PRKAR2A (PubMed:9852104). Interacts with ENO4 (PubMed:23446454).2 Publications

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050962.

Structurei

3D structure databases

ProteinModelPortaliQ60662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 232PKA-RI and PKA-RII subunit binding domainAdd BLAST14
Regioni335 – 344PKA-RI-alpha subunit binding domain10

Domaini

RI-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.1 Publication

Sequence similaritiesi

Belongs to the AKAP110 family.Curated

Phylogenomic databases

eggNOGiENOG410IKID. Eukaryota.
ENOG411157C. LUCA.
GeneTreeiENSGT00420000029845.
HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiQ60662.
KOiK16521.
OMAiKCLHHSI.
OrthoDBiEOG091G02HM.
PhylomeDBiQ60662.
TreeFamiTF105403.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 3 hits.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60662-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIAYCGTTTM SDDIDWLHSR RGVCKVDLYS PKGQQDQDRK VICFVDVSTL
60 70 80 90 100
NVEDKDSKGA AGSRSEGELN LETLEEKEII VIKDTEKQDQ SKTEGSVCLF
110 120 130 140 150
KQAPSDPISV LNWLLNDLQK YALGFQHALS PSASSCKHKV GDLEGDYSKI
160 170 180 190 200
PSENCYSVYA DQVNFDYLNK GPQNLRLEMA ASKNTNNNQS PSNPATKSPS
210 220 230 240 250
NQRSVATPEG ECSMDDLSFY VNRLSSLVIQ MARKEIKDKL EGGSKCLHHS
260 270 280 290 300
MYTSGDKGKT SPRSAVSKIA SEMAHEAVEL TSSEMRGNGE DCRDGRKTFL
310 320 330 340 350
YSEMCNKNKC GEKQQMCPKD SKEFADSISK GLMVYANQVA SDMMVSVMKT
360 370 380 390 400
LKVHSCGKPI PACVVLKRVL LKHTKEIVSD LIDSCMKNLH NITGVLMTDS
410 420 430 440 450
DFVSAVKRNL FNHGKQNAAD IMEAMLKRLV SALLGEKKET KSQSLAYATL
460 470 480 490 500
KAGTNDPKCK NQSLEFSAMK AEMKGKDKCK SKADPCCKSL TSAERVSEHI
510 520 530 540 550
LKESLTMWNN QKQGNQGKVT NKVCCTSKDE KREKISPSTD SLAKDLIVSA
560 570 580 590 600
LMLIQYHLTQ QAKGKDPCEE ECPGSSMGYM SQSAQYEKCG GGQSSKSLSM
610 620 630 640 650
KHFETRGAPG PSTCMKENQL ESQKMDMSNM VLSLIQKLLS ESPFSCDELT
660 670 680 690 700
ESDNKRCCDP RSSKAAPMAK RPEEQCQDNA ELDFISGMKQ MNRQFIDQLV
710 720 730 740 750
ESVMKLCLIM AKYSNNGAAL AELEEQAALV GSGSRCGRDA MMSQNYSETP
760 770 780 790 800
GPEVIVNNQC STTNLQKQLQ AVLQWIAASQ FNVPMLYFMG DDDGQLEKLP
810 820 830 840
EVSAKAAEKG YSVGDLLQEV MKFAKERQLD EAVGNMARKQ LLDWLLANL
Length:849
Mass (Da):93,796
Last modified:November 1, 1996 - v1
Checksum:i8DAC8939CF1CB81A
GO
Isoform 2 (identifier: Q60662-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Show »
Length:840
Mass (Da):92,854
Checksum:i38C1D7AFA330A198
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341S → E AA sequence (PubMed:7711182).Curated1
Sequence conflicti444S → D AA sequence (PubMed:7711182).Curated1
Sequence conflicti445L → T AA sequence (PubMed:7711182).Curated1
Sequence conflicti449T → P in AAA53216 (PubMed:8088444).Curated1
Sequence conflicti480K → T in AAA53216 (PubMed:8088444).Curated1
Sequence conflicti510N → T in AAA53216 (PubMed:8088444).Curated1
Sequence conflicti547I → T AA sequence (PubMed:7711182).Curated1
Sequence conflicti549S → P AA sequence (PubMed:7711182).Curated1
Sequence conflicti564G → A in AAA53216 (PubMed:8088444).Curated1
Sequence conflicti714S → R in AAA53216 (PubMed:8088444).Curated1
Sequence conflicti721A → G in AAA53216 (PubMed:8088444).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0041001 – 9Missing in isoform 2. Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10341 mRNA. Translation: AAA75597.1.
U07423 mRNA. Translation: AAA53216.1.
AF448786, AF448784, AF448785 Genomic DNA. Translation: AAM18539.1.
AF448786, AF448785 Genomic DNA. Translation: AAM18540.1.
AL808124 Genomic DNA. Translation: CAM15283.1.
CCDSiCCDS40838.1. [Q60662-1]
CCDS40839.1. [Q60662-2]
PIRiI49060.
RefSeqiNP_001036007.1. NM_001042542.2. [Q60662-2]
NP_033781.2. NM_009651.4. [Q60662-1]
UniGeneiMm.1498.

Genome annotation databases

EnsembliENSMUST00000057101; ENSMUSP00000050962; ENSMUSG00000050089. [Q60662-1]
ENSMUST00000115750; ENSMUSP00000111416; ENSMUSG00000050089. [Q60662-2]
ENSMUST00000115751; ENSMUSP00000111417; ENSMUSG00000050089. [Q60662-2]
GeneIDi11643.
KEGGimmu:11643.
UCSCiuc009slc.2. mouse. [Q60662-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10341 mRNA. Translation: AAA75597.1.
U07423 mRNA. Translation: AAA53216.1.
AF448786, AF448784, AF448785 Genomic DNA. Translation: AAM18539.1.
AF448786, AF448785 Genomic DNA. Translation: AAM18540.1.
AL808124 Genomic DNA. Translation: CAM15283.1.
CCDSiCCDS40838.1. [Q60662-1]
CCDS40839.1. [Q60662-2]
PIRiI49060.
RefSeqiNP_001036007.1. NM_001042542.2. [Q60662-2]
NP_033781.2. NM_009651.4. [Q60662-1]
UniGeneiMm.1498.

3D structure databases

ProteinModelPortaliQ60662.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050962.

PTM databases

iPTMnetiQ60662.
PhosphoSitePlusiQ60662.

Proteomic databases

PaxDbiQ60662.
PRIDEiQ60662.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057101; ENSMUSP00000050962; ENSMUSG00000050089. [Q60662-1]
ENSMUST00000115750; ENSMUSP00000111416; ENSMUSG00000050089. [Q60662-2]
ENSMUST00000115751; ENSMUSP00000111417; ENSMUSG00000050089. [Q60662-2]
GeneIDi11643.
KEGGimmu:11643.
UCSCiuc009slc.2. mouse. [Q60662-1]

Organism-specific databases

CTDi8852.
MGIiMGI:102794. Akap4.

Phylogenomic databases

eggNOGiENOG410IKID. Eukaryota.
ENOG411157C. LUCA.
GeneTreeiENSGT00420000029845.
HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiQ60662.
KOiK16521.
OMAiKCLHHSI.
OrthoDBiEOG091G02HM.
PhylomeDBiQ60662.
TreeFamiTF105403.

Miscellaneous databases

ChiTaRSiAkap4. mouse.
PROiQ60662.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050089.
CleanExiMM_AKAP4.
ExpressionAtlasiQ60662. baseline and differential.
GenevisibleiQ60662. MM.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 3 hits.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKAP4_MOUSE
AccessioniPrimary (citable) accession number: Q60662
Secondary accession number(s): B1AXM7, Q60630, Q8R2G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.