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Q60644 (NR1H2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Oxysterols receptor LXR-beta
Alternative name(s):
Liver X receptor beta
Nuclear receptor subfamily 1 group H member 2
Retinoid X receptor-interacting protein No.15
Ubiquitously-expressed nuclear receptor
Gene names
Name:Nr1h2
Synonyms:Lxrb, Rip15, Unr, Unr2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Nuclear receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Ref.4 Ref.5 Ref.6

Subunit structure

Forms a heterodimer with RXR.

Subcellular location

Nucleus Potential.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionReceptor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular lipid metabolic process

Inferred from direct assay PubMed 14739254. Source: MGI

cholesterol homeostasis

Inferred from direct assay PubMed 17657314. Source: BHF-UCL

lipid homeostasis

Inferred from mutant phenotype PubMed 18806227. Source: BHF-UCL

negative regulation of cholesterol storage

Inferred from electronic annotation. Source: Ensembl

negative regulation of lipid transport

Inferred from electronic annotation. Source: Ensembl

negative regulation of pinocytosis

Inferred from electronic annotation. Source: Ensembl

negative regulation of proteolysis

Inferred from mutant phenotype PubMed 18806227. Source: BHF-UCL

negative regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 19329817. Source: BHF-UCL

positive regulation of cellular protein metabolic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cholesterol efflux

Inferred from direct assay PubMed 16825483PubMed 17657314. Source: BHF-UCL

positive regulation of fatty acid biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of high-density lipoprotein particle assembly

Inferred from direct assay PubMed 16825483PubMed 17657314. Source: BHF-UCL

positive regulation of lipid storage

Inferred from mutant phenotype PubMed 18806227. Source: BHF-UCL

positive regulation of lipoprotein lipase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of pancreatic juice secretion

Inferred from mutant phenotype PubMed 18806227. Source: BHF-UCL

positive regulation of secretion of lysosomal enzymes

Inferred from mutant phenotype PubMed 18806227. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16141411PubMed 16825483. Source: BHF-UCL

positive regulation of triglyceride biosynthetic process

Inferred from electronic annotation. Source: Ensembl

regulation of cholesterol homeostasis

Inferred from mutant phenotype Ref.4. Source: UniProtKB

regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction Ref.1. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay PubMed 14739254. Source: MGI

retinoic acid receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 18806227. Source: BHF-UCL

   Molecular_functionDNA binding

Inferred from direct assay PubMed 14739254. Source: MGI

ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from direct assay PubMed 14739254. Source: MGI

protein binding

Inferred from physical interaction PubMed 17936707. Source: IntAct

retinoid X receptor binding

Inferred from direct assay Ref.1. Source: MGI

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 14739254. Source: MGI

sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity

Inferred from electronic annotation. Source: Ensembl

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SIRT1Q96EB62EBI-5276809,EBI-1802965From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Oxysterols receptor LXR-beta
PRO_0000053533

Regions

DNA binding75 – 15278Nuclear receptor
Zinc finger78 – 9821NR C4-type
Zinc finger116 – 14025NR C4-type
Region216 – 446231Ligand-binding Potential
Compositional bias162 – 1687Poly-Gln
Compositional bias169 – 1724Poly-Pro

Sequences

Sequence LengthMass (Da)Tools
Q60644 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 73153E635302C9DF

FASTA44649,720
        10         20         30         40         50         60 
MSSPTSSLDT PVPGNGSPQP STSATSPTIK EEGQETDPPP GSEGSSSAYI VVILEPEDEP 

        70         80         90        100        110        120 
ERKRKKGPAP KMLGHELCRV CGDKASGFHY NVLSCEGCKG FFRRSVVHGG AGRYACRGSG 

       130        140        150        160        170        180 
TCQMDAFMRR KCQLCRLRKC KEAGMREQCV LSEEQIRKKR IQKQQQQQPP PPSEPAASSS 

       190        200        210        220        230        240 
GRPAASPGTS EASSQGSGEG EGIQLTAAQE LMIQQLVAAQ LQCNKRSFSD QPKVTPWPLG 

       250        260        270        280        290        300 
ADPQSRDARQ QRFAHFTELA IISVQEIVDF AKQVPGFLQL GREDQIALLK ASTIEIMLLE 

       310        320        330        340        350        360 
TARRYNHETE CITFLKDFTY SKDDFHRAGL QVEFINPIFE FSRAMRRLGL DDAEYALLIA 

       370        380        390        400        410        420 
INIFSADRPN VQEPSRVEAL QQPYVEALLS YTRIKRPQDQ LRFPRMLMKL VSLRTLSSVH 

       430        440 
SEQVFALRLQ DKKLPPLLSE IWDVHE 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of proteins that interact specifically with the retinoid X receptor: two novel orphan receptors."
Seol W., Choi H.S., Moore D.D.
Mol. Endocrinol. 9:72-85(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Structural characterisation of the mouse nuclear oxysterol receptor genes LXRalpha and LXRbeta."
Alberti S., Steffensen K.R., Gustafsson J.-A.
Gene 243:93-103(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Identification of oxysterol 7alpha-hydroxylase (Cyp7b1) as a novel retinoid-related orphan receptor alpha (RORalpha) (NR1F1) target gene and a functional cross-talk between RORalpha and liver X receptor (NR1H3)."
Wada T., Kang H.S., Angers M., Gong H., Bhatia S., Khadem S., Ren S., Ellis E., Strom S.C., Jetten A.M., Xie W.
Mol. Pharmacol. 73:891-899(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN METABOLISM REGULATION.
[5]"LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor."
Zelcer N., Hong C., Boyadjian R., Tontonoz P.
Science 325:100-104(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The E3 ubiquitin ligase IDOL induces the degradation of the low density lipoprotein receptor family members VLDLR and ApoER2."
Hong C., Duit S., Jalonen P., Out R., Scheer L., Sorrentino V., Boyadjian R., Rodenburg K.W., Foley E., Korhonen L., Lindholm D., Nimpf J., van Berkel T.J., Tontonoz P., Zelcer N.
J. Biol. Chem. 285:19720-19726(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09419 mRNA. Translation: AAC52164.1.
AJ132602 Genomic DNA. Translation: CAB51924.1.
BC066025 mRNA. Translation: AAH66025.1.
CCDSCCDS21211.1.
PIRI49021.
RefSeqNP_001272446.1. NM_001285517.1.
NP_001272447.1. NM_001285518.1.
NP_001272448.1. NM_001285519.1.
NP_033499.1. NM_009473.3.
XP_006540867.1. XM_006540804.1.
UniGeneMm.968.

3D structure databases

ProteinModelPortalQ60644.
SMRQ60644. Positions 66-445.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204450. 1 interaction.
DIPDIP-444N.
IntActQ60644. 2 interactions.
STRING10090.ENSMUSP00000103545.

Chemistry

ChEMBLCHEMBL2417346.

PTM databases

PhosphoSiteQ60644.

Proteomic databases

PRIDEQ60644.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000073488; ENSMUSP00000073188; ENSMUSG00000060601.
ENSMUST00000107912; ENSMUSP00000103545; ENSMUSG00000060601.
ENSMUST00000167197; ENSMUSP00000126788; ENSMUSG00000060601.
GeneID22260.
KEGGmmu:22260.
UCSCuc009gpz.1. mouse.

Organism-specific databases

CTD7376.
MGIMGI:1352463. Nr1h2.

Phylogenomic databases

eggNOGNOG285805.
GeneTreeENSGT00720000108423.
HOGENOMHOG000220845.
HOVERGENHBG108655.
InParanoidQ60644.
KOK08535.
OMARRYACRG.
OrthoDBEOG7DC25S.
PhylomeDBQ60644.

Gene expression databases

ArrayExpressQ60644.
BgeeQ60644.
CleanExMM_NR1H2.
GenevestigatorQ60644.

Family and domain databases

Gene3D1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProIPR023257. Liver_X_rcpt.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSPR02034. LIVERXRECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNR1H2. mouse.
NextBio302353.
PROQ60644.
SOURCESearch...

Entry information

Entry nameNR1H2_MOUSE
AccessionPrimary (citable) accession number: Q60644
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot