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Protein

Oxysterols receptor LXR-beta

Gene

Nr1h2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Exhibits a ligand-dependent transcriptional activation activity (By similarity).By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi75 – 15278Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri78 – 9821NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri116 – 14025NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cellular lipid metabolic process Source: MGI
  • cholesterol homeostasis Source: BHF-UCL
  • lipid homeostasis Source: BHF-UCL
  • negative regulation of cholesterol storage Source: MGI
  • negative regulation of lipid transport Source: MGI
  • negative regulation of pinocytosis Source: MGI
  • negative regulation of proteolysis Source: BHF-UCL
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of cellular protein metabolic process Source: MGI
  • positive regulation of cholesterol efflux Source: BHF-UCL
  • positive regulation of cholesterol transport Source: MGI
  • positive regulation of fatty acid biosynthetic process Source: MGI
  • positive regulation of high-density lipoprotein particle assembly Source: BHF-UCL
  • positive regulation of lipid storage Source: BHF-UCL
  • positive regulation of lipoprotein lipase activity Source: MGI
  • positive regulation of pancreatic juice secretion Source: BHF-UCL
  • positive regulation of secretion of lysosomal enzymes Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of triglyceride biosynthetic process Source: MGI
  • regulation of cholesterol homeostasis Source: UniProtKB
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • retinoic acid receptor signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_276696. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterols receptor LXR-beta
Alternative name(s):
Liver X receptor beta
Nuclear receptor subfamily 1 group H member 2
Retinoid X receptor-interacting protein No.15
Ubiquitously-expressed nuclear receptor
Gene namesi
Name:Nr1h2
Synonyms:Lxrb, Rip15, Unr, Unr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1352463. Nr1h2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Oxysterols receptor LXR-betaPRO_0000053533Add
BLAST

Proteomic databases

PRIDEiQ60644.

PTM databases

PhosphoSiteiQ60644.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiQ60644.
CleanExiMM_NR1H2.
ExpressionAtlasiQ60644. baseline and differential.
GenevisibleiQ60644. MM.

Interactioni

Subunit structurei

Forms a heterodimer with RXR. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SIRT1Q96EB62EBI-5276809,EBI-1802965From a different organism.

Protein-protein interaction databases

BioGridi204450. 1 interaction.
DIPiDIP-444N.
IntActiQ60644. 4 interactions.
STRINGi10090.ENSMUSP00000073188.

Structurei

3D structure databases

ProteinModelPortaliQ60644.
SMRiQ60644. Positions 66-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7676Transactivation AF-1; required for ligand-independent transactivation functionBy similarityAdd
BLAST
Regioni205 – 446242Transactivation AF-2; required for ligand-dependent transactivation function; mediates interaction with CCAR2By similarityAdd
BLAST
Regioni216 – 446231Ligand-bindingSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi162 – 1687Poly-Gln
Compositional biasi169 – 1724Poly-Pro

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri78 – 9821NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri116 – 14025NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG285805.
GeneTreeiENSGT00780000121834.
HOGENOMiHOG000220845.
HOVERGENiHBG108655.
InParanoidiQ60644.
KOiK08535.
OMAiRRYACRG.
OrthoDBiEOG7DC25S.
PhylomeDBiQ60644.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR023257. Liver_X_rcpt.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR02034. LIVERXRECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPTSSLDT PVPGNGSPQP STSATSPTIK EEGQETDPPP GSEGSSSAYI
60 70 80 90 100
VVILEPEDEP ERKRKKGPAP KMLGHELCRV CGDKASGFHY NVLSCEGCKG
110 120 130 140 150
FFRRSVVHGG AGRYACRGSG TCQMDAFMRR KCQLCRLRKC KEAGMREQCV
160 170 180 190 200
LSEEQIRKKR IQKQQQQQPP PPSEPAASSS GRPAASPGTS EASSQGSGEG
210 220 230 240 250
EGIQLTAAQE LMIQQLVAAQ LQCNKRSFSD QPKVTPWPLG ADPQSRDARQ
260 270 280 290 300
QRFAHFTELA IISVQEIVDF AKQVPGFLQL GREDQIALLK ASTIEIMLLE
310 320 330 340 350
TARRYNHETE CITFLKDFTY SKDDFHRAGL QVEFINPIFE FSRAMRRLGL
360 370 380 390 400
DDAEYALLIA INIFSADRPN VQEPSRVEAL QQPYVEALLS YTRIKRPQDQ
410 420 430 440
LRFPRMLMKL VSLRTLSSVH SEQVFALRLQ DKKLPPLLSE IWDVHE
Length:446
Mass (Da):49,720
Last modified:November 1, 1996 - v1
Checksum:i73153E635302C9DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09419 mRNA. Translation: AAC52164.1.
AJ132602 Genomic DNA. Translation: CAB51924.1.
BC066025 mRNA. Translation: AAH66025.1.
CCDSiCCDS21211.1.
PIRiI49021.
RefSeqiNP_001272446.1. NM_001285517.1.
NP_001272447.1. NM_001285518.1.
NP_001272448.1. NM_001285519.1.
NP_033499.1. NM_009473.3.
XP_006540867.1. XM_006540804.2.
UniGeneiMm.968.

Genome annotation databases

EnsembliENSMUST00000073488; ENSMUSP00000073188; ENSMUSG00000060601.
ENSMUST00000107912; ENSMUSP00000103545; ENSMUSG00000060601.
ENSMUST00000167197; ENSMUSP00000126788; ENSMUSG00000060601.
GeneIDi22260.
KEGGimmu:22260.
UCSCiuc009gpz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09419 mRNA. Translation: AAC52164.1.
AJ132602 Genomic DNA. Translation: CAB51924.1.
BC066025 mRNA. Translation: AAH66025.1.
CCDSiCCDS21211.1.
PIRiI49021.
RefSeqiNP_001272446.1. NM_001285517.1.
NP_001272447.1. NM_001285518.1.
NP_001272448.1. NM_001285519.1.
NP_033499.1. NM_009473.3.
XP_006540867.1. XM_006540804.2.
UniGeneiMm.968.

3D structure databases

ProteinModelPortaliQ60644.
SMRiQ60644. Positions 66-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204450. 1 interaction.
DIPiDIP-444N.
IntActiQ60644. 4 interactions.
STRINGi10090.ENSMUSP00000073188.

Chemistry

ChEMBLiCHEMBL2417346.

PTM databases

PhosphoSiteiQ60644.

Proteomic databases

PRIDEiQ60644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073488; ENSMUSP00000073188; ENSMUSG00000060601.
ENSMUST00000107912; ENSMUSP00000103545; ENSMUSG00000060601.
ENSMUST00000167197; ENSMUSP00000126788; ENSMUSG00000060601.
GeneIDi22260.
KEGGimmu:22260.
UCSCiuc009gpz.1. mouse.

Organism-specific databases

CTDi7376.
MGIiMGI:1352463. Nr1h2.

Phylogenomic databases

eggNOGiNOG285805.
GeneTreeiENSGT00780000121834.
HOGENOMiHOG000220845.
HOVERGENiHBG108655.
InParanoidiQ60644.
KOiK08535.
OMAiRRYACRG.
OrthoDBiEOG7DC25S.
PhylomeDBiQ60644.

Enzyme and pathway databases

ReactomeiREACT_276696. Nuclear Receptor transcription pathway.

Miscellaneous databases

ChiTaRSiNr1h2. mouse.
NextBioi302353.
PROiQ60644.
SOURCEiSearch...

Gene expression databases

BgeeiQ60644.
CleanExiMM_NR1H2.
ExpressionAtlasiQ60644. baseline and differential.
GenevisibleiQ60644. MM.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR023257. Liver_X_rcpt.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR02034. LIVERXRECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of proteins that interact specifically with the retinoid X receptor: two novel orphan receptors."
    Seol W., Choi H.S., Moore D.D.
    Mol. Endocrinol. 9:72-85(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Structural characterisation of the mouse nuclear oxysterol receptor genes LXRalpha and LXRbeta."
    Alberti S., Steffensen K.R., Gustafsson J.-A.
    Gene 243:93-103(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Identification of oxysterol 7alpha-hydroxylase (Cyp7b1) as a novel retinoid-related orphan receptor alpha (RORalpha) (NR1F1) target gene and a functional cross-talk between RORalpha and liver X receptor (NR1H3)."
    Wada T., Kang H.S., Angers M., Gong H., Bhatia S., Khadem S., Ren S., Ellis E., Strom S.C., Jetten A.M., Xie W.
    Mol. Pharmacol. 73:891-899(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN METABOLISM REGULATION.
  5. "LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor."
    Zelcer N., Hong C., Boyadjian R., Tontonoz P.
    Science 325:100-104(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The E3 ubiquitin ligase IDOL induces the degradation of the low density lipoprotein receptor family members VLDLR and ApoER2."
    Hong C., Duit S., Jalonen P., Out R., Scheer L., Sorrentino V., Boyadjian R., Rodenburg K.W., Foley E., Korhonen L., Lindholm D., Nimpf J., van Berkel T.J., Tontonoz P., Zelcer N.
    J. Biol. Chem. 285:19720-19726(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNR1H2_MOUSE
AccessioniPrimary (citable) accession number: Q60644
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.