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Protein

Flotillin-2

Gene

Flot2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-8849932. SALM protein interactions at the synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Flotillin-2
Alternative name(s):
Epidermal surface antigen
Short name:
ESA
Membrane component chromosome 17 surface marker 1 homolog
Gene namesi
Name:Flot2
Synonyms:Esa1, M17s1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:103309. Flot2.

Subcellular locationi

GO - Cellular componenti

  • acrosomal membrane Source: MGI
  • basolateral plasma membrane Source: MGI
  • caveola Source: MGI
  • cell-cell adherens junction Source: MGI
  • cell-cell contact zone Source: MGI
  • cortical actin cytoskeleton Source: Ensembl
  • cytoplasmic vesicle Source: UniProtKB
  • endocytic vesicle Source: UniProtKB
  • endosome Source: UniProtKB
  • extracellular exosome Source: MGI
  • flotillin complex Source: UniProtKB
  • focal adhesion Source: MGI
  • lamellipodium Source: MGI
  • membrane Source: MGI
  • membrane raft Source: MGI
  • perinuclear region of cytoplasm Source: MGI
  • plasma membrane Source: MGI
  • uropod Source: MGI
  • vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000940502 – 428Flotillin-2Add BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi4S-palmitoyl cysteine; by ZDHHC5By similarity1
Lipidationi19S-palmitoyl cysteineBy similarity1
Lipidationi20S-palmitoyl cysteine; by ZDHHC5By similarity1
Modified residuei405PhosphoserineBy similarity1

Post-translational modificationi

ZDHHC5-catalyzed palmitoylation may be required for the formation of higher-order complexes and for neurite outgrowth in cultured neural stem cells.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ60634.
PeptideAtlasiQ60634.
PRIDEiQ60634.

PTM databases

iPTMnetiQ60634.
PhosphoSitePlusiQ60634.
SwissPalmiQ60634.

Expressioni

Tissue specificityi

Expressed in many tissues, including suprabasal epidermis, hair follicles, heart, lung, thymus, spleen, liver, kidney and brain. Not expressed in skeletal muscle.

Gene expression databases

BgeeiENSMUSG00000061981.
CleanExiMM_FLOT2.
ExpressionAtlasiQ60634. baseline and differential.
GenevisibleiQ60634. MM.

Interactioni

Subunit structurei

Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199705. 57 interactors.
IntActiQ60634. 61 interactors.
MINTiMINT-2982972.
STRINGi10090.ENSMUSP00000072136.

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi50 – 52Combined sources3
Beta strandi56 – 60Combined sources5
Beta strandi62 – 64Combined sources3
Beta strandi66 – 69Combined sources4
Beta strandi72 – 77Combined sources6
Helixi85 – 91Combined sources7
Beta strandi92 – 94Combined sources3
Helixi96 – 117Combined sources22
Helixi120 – 125Combined sources6
Helixi127 – 142Combined sources16
Turni143 – 146Combined sources4
Beta strandi147 – 153Combined sources7
Helixi163 – 167Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WINNMR-A43-172[»]
ProteinModelPortaliQ60634.
SMRiQ60634.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60634.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2668. Eukaryota.
COG2268. LUCA.
GeneTreeiENSGT00560000077232.
HOGENOMiHOG000240804.
HOVERGENiHBG051628.
InParanoidiQ60634.
KOiK07192.
OMAiIRCIGEA.
TreeFamiTF324879.

Family and domain databases

InterProiIPR001107. Band_7.
IPR031905. Flotillin_C.
IPR027705. Flotillin_fam.
[Graphical view]
PANTHERiPTHR13806. PTHR13806. 1 hit.
PfamiPF01145. Band_7. 1 hit.
PF15975. Flot. 1 hit.
[Graphical view]
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60634-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNCHTVGPN EALVVSGGCC GSDYKQYVFG GWAWAWWCIS DTQRISLEIM
60 70 80 90 100
TLQPRCEDVE TAEGVALTVT GVAQVKIMTE KELLAVACEQ FLGKNVQDIK
110 120 130 140 150
NVVLQTLEGH LRSILGTLTV EQIYQDRDQF AKLVREVAAP DVGRMGIEIL
160 170 180 190 200
SFTIKDVYDK VDYLSSLGKT QTAVVQRDAD IGVAEAERDA GIREAECKKE
210 220 230 240 250
MLDVKFMADT KIADSKRAFE LQKSAFSEEV NIKTAEAQLA YELQGAREQQ
260 270 280 290 300
KIRQEEIEIE VVQRKKQIAV EAQEILRTDK ELIATVRRPA EAEAHRIQQI
310 320 330 340 350
AEGEKVKQVL LAQAEAEKIR KIGEAEAAVI EAMGKAEAER MKLKAEAYQK
360 370 380 390 400
YGDAAKMALV LEALPQIAAK ISAPLTKVDE IVVLSGDNSK VTSEVNRLLA
410 420
ELPASVHALT GVDLSKIPLI KNATGAQV
Length:428
Mass (Da):47,038
Last modified:July 28, 2009 - v2
Checksum:iE482A0E2071D3CA7
GO
Isoform 2 (identifier: Q60634-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-68: MTLQPRCEDVETAEGVALT → MTILCRCENIETSEGVPLF

Show »
Length:428
Mass (Da):47,130
Checksum:i7CC8BF0665890E0F
GO
Isoform 3 (identifier: Q60634-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Show »
Length:379
Mass (Da):41,659
Checksum:i043869D46EFEE3DE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87A → S in AAH70423 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0376921 – 49Missing in isoform 3. 2 PublicationsAdd BLAST49
Alternative sequenceiVSP_00050250 – 68MTLQP…GVALT → MTILCRCENIETSEGVPLF in isoform 2. CuratedAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07890 mRNA. Translation: AAA93127.1.
AK170557 mRNA. Translation: BAE41879.1.
AL669840 Genomic DNA. Translation: CAI25705.1.
CH466596 Genomic DNA. Translation: EDL12906.1.
BC070423 mRNA. Translation: AAH70423.1.
CCDSiCCDS25088.1. [Q60634-3]
CCDS36237.1. [Q60634-1]
RefSeqiNP_001035493.1. NM_001040403.1. [Q60634-1]
NP_001271156.1. NM_001284227.1.
NP_001271157.1. NM_001284228.1. [Q60634-3]
NP_032054.1. NM_008028.2. [Q60634-3]
XP_006532255.1. XM_006532192.2. [Q60634-3]
XP_006532256.1. XM_006532193.3. [Q60634-3]
UniGeneiMm.130227.

Genome annotation databases

EnsembliENSMUST00000072289; ENSMUSP00000072136; ENSMUSG00000061981. [Q60634-1]
ENSMUST00000100784; ENSMUSP00000098347; ENSMUSG00000061981. [Q60634-3]
GeneIDi14252.
KEGGimmu:14252.
UCSCiuc007khw.1. mouse. [Q60634-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07890 mRNA. Translation: AAA93127.1.
AK170557 mRNA. Translation: BAE41879.1.
AL669840 Genomic DNA. Translation: CAI25705.1.
CH466596 Genomic DNA. Translation: EDL12906.1.
BC070423 mRNA. Translation: AAH70423.1.
CCDSiCCDS25088.1. [Q60634-3]
CCDS36237.1. [Q60634-1]
RefSeqiNP_001035493.1. NM_001040403.1. [Q60634-1]
NP_001271156.1. NM_001284227.1.
NP_001271157.1. NM_001284228.1. [Q60634-3]
NP_032054.1. NM_008028.2. [Q60634-3]
XP_006532255.1. XM_006532192.2. [Q60634-3]
XP_006532256.1. XM_006532193.3. [Q60634-3]
UniGeneiMm.130227.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WINNMR-A43-172[»]
ProteinModelPortaliQ60634.
SMRiQ60634.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199705. 57 interactors.
IntActiQ60634. 61 interactors.
MINTiMINT-2982972.
STRINGi10090.ENSMUSP00000072136.

PTM databases

iPTMnetiQ60634.
PhosphoSitePlusiQ60634.
SwissPalmiQ60634.

Proteomic databases

PaxDbiQ60634.
PeptideAtlasiQ60634.
PRIDEiQ60634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072289; ENSMUSP00000072136; ENSMUSG00000061981. [Q60634-1]
ENSMUST00000100784; ENSMUSP00000098347; ENSMUSG00000061981. [Q60634-3]
GeneIDi14252.
KEGGimmu:14252.
UCSCiuc007khw.1. mouse. [Q60634-1]

Organism-specific databases

CTDi2319.
MGIiMGI:103309. Flot2.

Phylogenomic databases

eggNOGiKOG2668. Eukaryota.
COG2268. LUCA.
GeneTreeiENSGT00560000077232.
HOGENOMiHOG000240804.
HOVERGENiHBG051628.
InParanoidiQ60634.
KOiK07192.
OMAiIRCIGEA.
TreeFamiTF324879.

Enzyme and pathway databases

ReactomeiR-MMU-8849932. SALM protein interactions at the synapses.

Miscellaneous databases

EvolutionaryTraceiQ60634.
PROiQ60634.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061981.
CleanExiMM_FLOT2.
ExpressionAtlasiQ60634. baseline and differential.
GenevisibleiQ60634. MM.

Family and domain databases

InterProiIPR001107. Band_7.
IPR031905. Flotillin_C.
IPR027705. Flotillin_fam.
[Graphical view]
PANTHERiPTHR13806. PTHR13806. 1 hit.
PfamiPF01145. Band_7. 1 hit.
PF15975. Flot. 1 hit.
[Graphical view]
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFLOT2_MOUSE
AccessioniPrimary (citable) accession number: Q60634
Secondary accession number(s): Q5SS82, Q6NS75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 28, 2009
Last modified: November 2, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.