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Q60631

- GRB2_MOUSE

UniProt

Q60631 - GRB2_MOUSE

Protein

Growth factor receptor-bound protein 2

Gene

Grb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 159 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced. transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death By similarity.By similarity

    GO - Molecular functioni

    1. ephrin receptor binding Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. protein domain specific binding Source: MGI
    4. protein phosphatase binding Source: UniProtKB
    5. SH3/SH2 adaptor activity Source: MGI
    6. SH3 domain binding Source: UniProtKB

    GO - Biological processi

    1. aging Source: Ensembl
    2. anatomical structure formation involved in morphogenesis Source: MGI
    3. branching involved in labyrinthine layer morphogenesis Source: MGI
    4. cell differentiation Source: MGI
    5. cellular response to ionizing radiation Source: Ensembl
    6. insulin receptor signaling pathway Source: Ensembl
    7. positive regulation of actin filament polymerization Source: MGI
    8. positive regulation of reactive oxygen species metabolic process Source: Ensembl
    9. positive regulation of signal transduction Source: GOC
    10. protein heterooligomerization Source: Ensembl
    11. Ras protein signal transduction Source: MGI
    12. receptor internalization Source: Ensembl
    13. regulation of MAPK cascade Source: MGI
    14. signal transduction in response to DNA damage Source: Ensembl

    Enzyme and pathway databases

    ReactomeiREACT_188185. DAP12 signaling.
    REACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_188202. FCERI mediated Ca+2 mobilization.
    REACT_188528. GRB2 events in ERBB2 signaling.
    REACT_188530. FCERI mediated MAPK activation.
    REACT_188574. SHC1 events in ERBB2 signaling.
    REACT_188578. Signaling by SCF-KIT.
    REACT_188580. SHC1 events in ERBB4 signaling.
    REACT_196455. Signaling by FGFR mutants.
    REACT_196460. Signaling by FGFR1 fusion mutants.
    REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_198350. EGFR Transactivation by Gastrin.
    REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_198525. Negative regulation of FGFR signaling.
    REACT_198526. Spry regulation of FGF signaling.
    REACT_198634. Regulation of signaling by CBL.
    REACT_199123. Signaling by constitutively active EGFR.
    REACT_203917. EGFR downregulation.
    REACT_206286. GAB1 signalosome.
    REACT_210793. Interleukin receptor SHC signaling.
    REACT_211860. Tie2 Signaling.
    REACT_213364. Role of LAT2/NTAL/LAB on calcium mobilization.
    REACT_215348. PI3K events in ERBB2 signaling.
    REACT_215628. Signal regulatory protein (SIRP) family interactions.
    REACT_223993. PI-3K cascade.
    REACT_225477. Costimulation by the CD28 family.
    REACT_225836. Interleukin-3, 5 and GM-CSF signaling.
    REACT_226341. PIP3 activates AKT signaling.
    REACT_227425. Regulation of KIT signaling.
    REACT_94437. SHC-mediated cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Growth factor receptor-bound protein 2
    Alternative name(s):
    Adapter protein GRB2
    SH2/SH3 adapter GRB2
    Gene namesi
    Name:Grb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:95805. Grb2.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity. Endosome By similarity. Golgi apparatus 1 Publication

    GO - Cellular componenti

    1. cell-cell junction Source: MGI
    2. COP9 signalosome Source: UniProtKB
    3. cytoplasm Source: MGI
    4. cytosol Source: Reactome
    5. endosome Source: UniProtKB
    6. Golgi apparatus Source: UniProtKB-SubCell
    7. Grb2-EGFR complex Source: Ensembl
    8. membrane Source: MGI
    9. nucleus Source: UniProtKB
    10. plasma membrane Source: MGI
    11. vesicle membrane Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Golgi apparatus, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 217217Growth factor receptor-bound protein 2PRO_0000088199Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei6 – 61N6-acetyllysineBy similarity
    Modified residuei50 – 501N6-acetyllysineBy similarity
    Modified residuei109 – 1091N6-acetyllysineBy similarity
    Modified residuei211 – 2111PhosphothreonineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ60631.
    PaxDbiQ60631.
    PRIDEiQ60631.

    PTM databases

    PhosphoSiteiQ60631.

    Expressioni

    Gene expression databases

    ArrayExpressiQ60631.
    BgeeiQ60631.
    CleanExiMM_GRB2.
    GenevestigatoriQ60631.

    Interactioni

    Subunit structurei

    Associates (via SH2 domain) with activated EGF receptors (tyrosine phosphorylated). Interacts with PDGFRA (tyrosine phosphorylated); the interaction may be indirect. Also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK; probably via the concerted action of both its SH2 and SH3 domains. It also seems to interact with RAS in the signaling pathway leading to DNA synthesis. Binds to and translocates the guanine nucleotide exchange factors SOS. Interacts with phosphorylated TOM1L1 and MET. Interacts with the phosphorylated C-terminus of SH2B2. Interacts with phosphorylated SIT1, LAX1, LAT, LAT2 and LIME1 upon TCR and/or BCR activation. Interacts with PTPNS1, REPS2 and the syntrophin SNTA1. Interacts with REPS1 and PIK3C2B via its SH3 domains. Interacts with CBL and CBLB. Interacts with AJUBA. Interacts with SHB, INPP5D/SHIP1, SKAP1 and SKAP2 By similarity. Interacts with CLNK. Forms a complex with MUC1 and SOS1, through interaction of the SH3 domains with SOS1 and the SH2 domain with phosphorylated MUC1. Interacts with PRNP By similarity. Interacts with NISCH and RALGPS1 By similarity. Interacts also with HCST. Interacts with GAPT and PTPRE. Interacts (via SH2 domain) with KIF26A By similarity. Interacts with THEMIS. Interacts (via SH3 2) with GAB2. Interacts with ADAM15. Interacts with PTPRJ and BCR. Interacts with PTPN23. Interacts with FLT4 (tyrosine phosphorylated) By similarity. Part of a complex including TNK2, GRB2 and one receptor tyrosine kinase (RTK) such as AXL, in which GRB2 promotes RTK recruitment by TNK2 By similarity. Interacts with NTRK1 (phosphorylated upon ligand-binding). Interacts with PTK2B/PYK2 (tyrosine phosphorylated) By similarity. Interacts with KDR. Interacts with FLT1 (tyrosine-phosphorylated). Interacts with THEMIS2. Interacts (via SH2 domain) with AXL and KIT (phosphorylated). Interacts with EPHB1 and SHC1; activates the MAPK/ERK cascade to regulate cell migration. Interacts with PTPN11. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Interacts with PTK2/FAK1 (tyrosine phosphorylated). Interacts with SCIMP. Interacts with TESPA1. Interacts (via SH3 domains) with GAREM (via proline-rich domain and tyrosine phosphorylated); the interaction occurs upon EGF stimulation. Interacts with ASAP3 (phosphorylated form). Interacts with CD28 By similarity. Interacts with PLCG1, LAT and THEMIS upon TCR activation in thymocytes; the association is weaker in the absence of TESPA1. Interacts with DAB2. Interacts with RAB13; may recruit RAB13 to the leading edge of migrating endothelial cells where it can activate RHOA.By similarity29 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BlnkO547374EBI-1688,EBI-641814
    BtkP359914EBI-1688,EBI-625119
    CblbB9EKI53EBI-1688,EBI-682463
    Cd22P353294EBI-1688,EBI-300059
    Dab2P980784EBI-1688,EBI-1391846
    Errfi1Q99JZ73EBI-1688,EBI-643375
    Frs2Q8C1805EBI-1688,EBI-6880000
    Gab1Q9QYY08EBI-1688,EBI-644784
    Kif26aQ52KG52EBI-1688,EBI-2480646
    LatO549572EBI-1688,EBI-6390034
    Plcg1Q620772EBI-1688,EBI-300133
    PrnpP049257EBI-1688,EBI-768613
    Ptpn11P352353EBI-1688,EBI-397236
    PtpraP180524EBI-1688,EBI-6597520
    Rapgef1Q91ZZ23EBI-1688,EBI-644719
    Shc1P980837EBI-1688,EBI-300201
    Sos1Q622457EBI-1688,EBI-1693
    Sos2Q023845EBI-1688,EBI-395573
    TekQ028583EBI-1688,EBI-7099626
    Uck2Q99PM93EBI-1688,EBI-644712

    Protein-protein interaction databases

    BioGridi200046. 28 interactions.
    DIPiDIP-259N.
    IntActiQ60631. 64 interactions.
    MINTiMINT-84889.

    Structurei

    Secondary structure

    1
    217
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 76
    Beta strandi13 – 164
    Beta strandi24 – 263
    Beta strandi34 – 418
    Beta strandi44 – 496
    Helixi50 – 523
    Beta strandi53 – 564

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GBQNMR-A1-61[»]
    1GBRNMR-A1-59[»]
    2GBQNMR-A1-59[»]
    3GBQNMR-A1-59[»]
    4GBQNMR-A1-59[»]
    ProteinModelPortaliQ60631.
    SMRiQ60631. Positions 1-217.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ60631.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 5858SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini60 – 15293SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini156 – 21560SH3 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The SH3 domains mediate interaction with RALGPS1 and SHB.By similarity

    Sequence similaritiesi

    Belongs to the GRB2/sem-5/DRK family.Curated
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 2 SH3 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiNOG298780.
    GeneTreeiENSGT00550000074482.
    HOGENOMiHOG000251625.
    HOVERGENiHBG005404.
    InParanoidiQ60631.
    KOiK04364.
    OMAiVETKFVQ.
    OrthoDBiEOG75F4F6.
    PhylomeDBiQ60631.
    TreeFamiTF354288.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00017. SH2. 1 hit.
    PF00018. SH3_1. 2 hits.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 2 hits.
    PROSITEiPS50001. SH2. 1 hit.
    PS50002. SH3. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: Q60631-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEAIAKYDFK ATADDELSFK RGDILKVLNE ECDQNWYKAE LNGKDGFIPK    50
    NYIEMKPHPW FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK 100
    FGNDVQHFKV LRDGAGKYFL WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD 150
    IEQMPQQPTY VQALFDFDPQ EDGELGFRRG DFIHVMDNSD PNWWKGACHG 200
    QTGMFPRNYV TPVNRNV 217
    Length:217
    Mass (Da):25,238
    Last modified:November 1, 1996 - v1
    Checksum:i97F4A4FE4B248DDF
    GO
    Isoform 2 (identifier: Q60631-2) [UniParc]FASTAAdd to Basket

    Also known as: GRB3-3

    The sequence of this isoform differs from the canonical sequence as follows:
         60-100: Missing.

    Show »
    Length:176
    Mass (Da):20,589
    Checksum:i64323FA95FE940C5
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei60 – 10041Missing in isoform 2. 1 PublicationVSP_001841Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07617 mRNA. Translation: AAB40022.1.
    D85748 mRNA. Translation: BAA12862.1.
    BC052377 mRNA. Translation: AAH52377.1.
    BC085254 mRNA. Translation: AAH85254.1.
    CCDSiCCDS25645.1. [Q60631-1]
    PIRiA54688.
    RefSeqiNP_032189.1. NM_008163.3. [Q60631-1]
    UniGeneiMm.439649.
    Mm.490413.

    Genome annotation databases

    EnsembliENSMUST00000021090; ENSMUSP00000021090; ENSMUSG00000059923. [Q60631-1]
    ENSMUST00000106497; ENSMUSP00000102106; ENSMUSG00000059923. [Q60631-1]
    ENSMUST00000106499; ENSMUSP00000102108; ENSMUSG00000059923. [Q60631-2]
    GeneIDi14784.
    KEGGimmu:14784.
    UCSCiuc007mij.1. mouse. [Q60631-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Grb2 entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07617 mRNA. Translation: AAB40022.1 .
    D85748 mRNA. Translation: BAA12862.1 .
    BC052377 mRNA. Translation: AAH52377.1 .
    BC085254 mRNA. Translation: AAH85254.1 .
    CCDSi CCDS25645.1. [Q60631-1 ]
    PIRi A54688.
    RefSeqi NP_032189.1. NM_008163.3. [Q60631-1 ]
    UniGenei Mm.439649.
    Mm.490413.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GBQ NMR - A 1-61 [» ]
    1GBR NMR - A 1-59 [» ]
    2GBQ NMR - A 1-59 [» ]
    3GBQ NMR - A 1-59 [» ]
    4GBQ NMR - A 1-59 [» ]
    ProteinModelPortali Q60631.
    SMRi Q60631. Positions 1-217.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200046. 28 interactions.
    DIPi DIP-259N.
    IntActi Q60631. 64 interactions.
    MINTi MINT-84889.

    Chemistry

    BindingDBi Q60631.
    ChEMBLi CHEMBL4830.

    PTM databases

    PhosphoSitei Q60631.

    Proteomic databases

    MaxQBi Q60631.
    PaxDbi Q60631.
    PRIDEi Q60631.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021090 ; ENSMUSP00000021090 ; ENSMUSG00000059923 . [Q60631-1 ]
    ENSMUST00000106497 ; ENSMUSP00000102106 ; ENSMUSG00000059923 . [Q60631-1 ]
    ENSMUST00000106499 ; ENSMUSP00000102108 ; ENSMUSG00000059923 . [Q60631-2 ]
    GeneIDi 14784.
    KEGGi mmu:14784.
    UCSCi uc007mij.1. mouse. [Q60631-1 ]

    Organism-specific databases

    CTDi 2885.
    MGIi MGI:95805. Grb2.

    Phylogenomic databases

    eggNOGi NOG298780.
    GeneTreei ENSGT00550000074482.
    HOGENOMi HOG000251625.
    HOVERGENi HBG005404.
    InParanoidi Q60631.
    KOi K04364.
    OMAi VETKFVQ.
    OrthoDBi EOG75F4F6.
    PhylomeDBi Q60631.
    TreeFami TF354288.

    Enzyme and pathway databases

    Reactomei REACT_188185. DAP12 signaling.
    REACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_188202. FCERI mediated Ca+2 mobilization.
    REACT_188528. GRB2 events in ERBB2 signaling.
    REACT_188530. FCERI mediated MAPK activation.
    REACT_188574. SHC1 events in ERBB2 signaling.
    REACT_188578. Signaling by SCF-KIT.
    REACT_188580. SHC1 events in ERBB4 signaling.
    REACT_196455. Signaling by FGFR mutants.
    REACT_196460. Signaling by FGFR1 fusion mutants.
    REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_198350. EGFR Transactivation by Gastrin.
    REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_198525. Negative regulation of FGFR signaling.
    REACT_198526. Spry regulation of FGF signaling.
    REACT_198634. Regulation of signaling by CBL.
    REACT_199123. Signaling by constitutively active EGFR.
    REACT_203917. EGFR downregulation.
    REACT_206286. GAB1 signalosome.
    REACT_210793. Interleukin receptor SHC signaling.
    REACT_211860. Tie2 Signaling.
    REACT_213364. Role of LAT2/NTAL/LAB on calcium mobilization.
    REACT_215348. PI3K events in ERBB2 signaling.
    REACT_215628. Signal regulatory protein (SIRP) family interactions.
    REACT_223993. PI-3K cascade.
    REACT_225477. Costimulation by the CD28 family.
    REACT_225836. Interleukin-3, 5 and GM-CSF signaling.
    REACT_226341. PIP3 activates AKT signaling.
    REACT_227425. Regulation of KIT signaling.
    REACT_94437. SHC-mediated cascade.

    Miscellaneous databases

    ChiTaRSi GRB2. mouse.
    EvolutionaryTracei Q60631.
    NextBioi 286907.
    PROi Q60631.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q60631.
    Bgeei Q60631.
    CleanExi MM_GRB2.
    Genevestigatori Q60631.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00017. SH2. 1 hit.
    PF00018. SH3_1. 2 hits.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 2 hits.
    PROSITEi PS50001. SH2. 1 hit.
    PS50002. SH3. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the mouse grb2 gene: differential interaction of the Grb2 adaptor protein with epidermal growth factor and nerve growth factor receptors."
      Suen K., Bustelo X.R., Pawson T., Barbacid M.
      Mol. Cell. Biol. 13:5500-5512(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. Tanaka S.
      Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: BALB/c.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary gland.
    4. Lubec G., Klug S.
      Submitted (MAR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 125-136, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Hippocampus.
    5. "Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase."
      Schlaepfer D.D., Hanks S.K., Hunter T., van der Geer P.
      Nature 372:786-791(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PTK2/FAK1.
    6. "Phosphorylation of tyrosine 720 in the platelet-derived growth factor alpha receptor is required for binding of Grb2 and SHP-2 but not for activation of Ras or cell proliferation."
      Bazenet C.E., Gelderloos J.A., Kazlauskas A.
      Mol. Cell. Biol. 16:6926-6936(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PDGFRA AND PTPN11.
    7. "Sequential activation of phoshatidylinositol 3-kinase and phospholipase C-gamma2 by the M-CSF receptor is necessary for differentiation signaling."
      Bourette R.P., Myles G.M., Choi J.L., Rohrschneider L.R.
      EMBO J. 16:5880-5893(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CSF1R, PHOSPHORYLATION.
    8. "An eps homology (EH) domain protein that binds to the ral-GTPase target, RalBP1."
      Yamaguchi A., Urano T., Goi T., Feig L.A.
      J. Biol. Chem. 272:31230-31234(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH REPS1.
      Tissue: Muscle.
    9. "Identification of vascular endothelial growth factor receptor-1 tyrosine phosphorylation sites and binding of SH2 domain-containing molecules."
      Ito N., Wernstedt C., Engstrom U., Claesson-Welsh L.
      J. Biol. Chem. 273:23410-23418(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FLT1.
    10. "Disabled-2 (Dab2) is an SH3 domain-binding partner of Grb2."
      Xu X.X., Yi T., Tang B., Lambeth J.D.
      Oncogene 16:1561-1569(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DAB2.
    11. "Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor."
      Thommes K., Lennartsson J., Carlberg M., Ronnstrand L.
      Biochem. J. 341:211-216(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KIT.
    12. "Gab-family adapter proteins act downstream of cytokine and growth factor receptors and T- and B-cell antigen receptors."
      Nishida K., Yoshida Y., Itoh M., Fukada T., Ohtani T., Shirogane T., Atsumi T., Takahashi-Tezuka M., Ishihara K., Hibi M., Hirano T.
      Blood 93:1809-1816(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GAB2.
    13. "Identification of Tek/Tie2 binding partners. Binding to a multifunctional docking site mediates cell survival and migration."
      Jones N., Master Z., Jones J., Bouchard D., Gunji Y., Sasaki H., Daly R., Alitalo K., Dumont D.J.
      J. Biol. Chem. 274:30896-30905(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TEK/TIE2.
    14. "Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner."
      Goyal R.K., Lin P., Kanungo J., Payne A.S., Muslin A.J., Longmore G.D.
      Mol. Cell. Biol. 19:4379-4389(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AJUBA.
    15. "Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling."
      Oak S.A., Russo K., Petrucci T.C., Jarrett H.W.
      Biochemistry 40:11270-11278(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNTA1.
    16. "MIST functions through distinct domains in immunoreceptor signaling in the presence and absence of LAT."
      Goitsuka R., Tatsuno A., Ishiai M., Kurosaki T., Kitamura D.
      J. Biol. Chem. 276:36043-36050(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CLNK.
    17. "PrPC directly interacts with proteins involved in signaling pathways."
      Spielhaupter C., Schaetzl H.M.
      J. Biol. Chem. 276:44604-44612(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PRNP.
    18. "Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and IRS-2-mediated signaling."
      Tsuruzoe K., Emkey R., Kriauciunas K.M., Ueki K., Kahn C.R.
      Mol. Cell. Biol. 21:26-38(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IRS4.
    19. "'Srcasm: a novel Src activating and signaling molecule."
      Seykora J.T., Mei L., Dotto G.P., Stein P.L.
      J. Biol. Chem. 277:2812-2822(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TOM1L1.
    20. "EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis."
      Vindis C., Cerretti D.P., Daniel T.O., Huynh-Do U.
      J. Cell Biol. 162:661-671(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EPHB1 AND SHC1.
    21. "LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation."
      Hur E.M., Son M., Lee O.-H., Choi Y.B., Park C., Lee H., Yun Y.
      J. Exp. Med. 198:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LIME1.
    22. "Positive and negative regulation of FcepsilonRI-mediated signaling by the adaptor protein LAB/NTAL."
      Zhu M., Liu Y., Koonpaew S., Granillo O., Zhang W.
      J. Exp. Med. 200:991-1000(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LAT2.
    23. Cited for: INTERACTION WITH LAT2.
    24. "LIME acts as a transmembrane adapter mediating BCR-dependent B-cell activation."
      Ahn E., Lee H., Yun Y.
      Blood 107:1521-1527(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LIME1.
    25. "Phosphorylation of Tyr1214 within VEGFR-2 triggers the recruitment of Nck and activation of Fyn leading to SAPK2/p38 activation and endothelial cell migration in response to VEGF."
      Lamalice L., Houle F., Huot J.
      J. Biol. Chem. 281:34009-34020(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KDR.
    26. "NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells."
      Upshaw J.L., Arneson L.N., Schoon R.A., Dick C.J., Billadeau D.D., Leibson P.J.
      Nat. Immunol. 7:524-532(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HCST.
    27. "Themis, a T cell-specific protein important for late thymocyte development."
      Lesourne R., Uehara S., Lee J., Song K.-D., Li L., Pinkhasov J., Zhang Y., Weng N.-P., Wildt K.F., Wang L., Bosselut R., Love P.E.
      Nat. Immunol. 10:840-847(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THEMIS.
    28. "Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection."
      Johnson A.L., Aravind L., Shulzhenko N., Morgun A., Choi S.-Y., Crockford T.L., Lambe T., Domaschenz H., Kucharska E.M., Zheng L., Vinuesa C.G., Lenardo M.J., Goodnow C.C., Cornall R.J., Schwartz R.H.
      Nat. Immunol. 10:831-839(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THEMIS.
    29. Cited for: INTERACTION WITH THEMIS.
    30. "Themis2/ICB1 is a signaling scaffold that selectively regulates macrophage Toll-like receptor signaling and cytokine production."
      Peirce M.J., Brook M., Morrice N., Snelgrove R., Begum S., Lanfrancotti A., Notley C., Hussell T., Cope A.P., Wait R.
      PLoS ONE 5:E11465-E11465(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THEMIS2.
    31. "Rab13-dependent trafficking of RhoA is required for directional migration and angiogenesis."
      Wu C., Agrawal S., Vasanji A., Drazba J., Sarkaria S., Xie J., Welch C.M., Liu M., Anand-Apte B., Horowitz A.
      J. Biol. Chem. 286:23511-23520(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAB13.
    32. "SCIMP, a transmembrane adapter protein involved in major histocompatibility complex class II signaling."
      Draber P., Vonkova I., Stepanek O., Hrdinka M., Kucova M., Skopcova T., Otahal P., Angelisova P., Horejsi V., Yeung M., Weiss A., Brdicka T.
      Mol. Cell. Biol. 31:4550-4562(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SCIMP.
    33. "Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling."
      Wang D., Zheng M., Lei L., Ji J., Yao Y., Qiu Y., Ma L., Lou J., Ouyang C., Zhang X., He Y., Chi J., Wang L., Kuang Y., Wang J., Cao X., Lu L.
      Nat. Immunol. 13:560-568(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LAT; PLCG1 AND THEMIS.
      Tissue: Thymocyte.
    34. "Solution structure of the Grb2 N-terminal SH3 domain complexed with a ten-residue peptide derived from SOS: direct refinement against NOEs, J-couplings and 1H and 13C chemical shifts."
      Wittekind M., Mapelli C., Lee V., Goldfarb V., Friedrichs M.S., Meyers C.A., Mueller L.
      J. Mol. Biol. 267:933-952(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 1-65.

    Entry informationi

    Entry nameiGRB2_MOUSE
    AccessioniPrimary (citable) accession number: Q60631
    Secondary accession number(s): Q61240
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 159 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3