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Protein

Adenosine receptor A2b

Gene

Adora2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

GO - Molecular functioni

  1. G-protein coupled adenosine receptor activity Source: InterPro
  2. G-protein coupled receptor activity Source: MGI

GO - Biological processi

  1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
  2. cellular response to extracellular stimulus Source: MGI
  3. positive regulation of cAMP biosynthetic process Source: MGI
  4. positive regulation of cGMP biosynthetic process Source: MGI
  5. positive regulation of chemokine production Source: MGI
  6. positive regulation of chronic inflammatory response to non-antigenic stimulus Source: MGI
  7. positive regulation of guanylate cyclase activity Source: MGI
  8. positive regulation of interleukin-6 production Source: MGI
  9. positive regulation of mast cell degranulation Source: MGI
  10. positive regulation vascular endothelial growth factor production Source: MGI
  11. relaxation of vascular smooth muscle Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_242871. G alpha (s) signalling events.
REACT_253800. Adenosine P1 receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine receptor A2b
Gene namesi
Name:Adora2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:99403. Adora2b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88ExtracellularBy similarity
Transmembranei9 – 3325Helical; Name=1By similarityAdd
BLAST
Topological domaini34 – 4310CytoplasmicBy similarity
Transmembranei44 – 6724Helical; Name=2By similarityAdd
BLAST
Topological domaini68 – 7811ExtracellularBy similarityAdd
BLAST
Transmembranei79 – 10123Helical; Name=3By similarityAdd
BLAST
Topological domaini102 – 12120CytoplasmicBy similarityAdd
BLAST
Transmembranei122 – 14423Helical; Name=4By similarityAdd
BLAST
Topological domaini145 – 17834ExtracellularBy similarityAdd
BLAST
Transmembranei179 – 20325Helical; Name=5By similarityAdd
BLAST
Topological domaini204 – 23532CytoplasmicBy similarityAdd
BLAST
Transmembranei236 – 25924Helical; Name=6By similarityAdd
BLAST
Topological domaini260 – 2678ExtracellularBy similarity
Transmembranei268 – 29124Helical; Name=7By similarityAdd
BLAST
Topological domaini292 – 33241CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Adenosine receptor A2bPRO_0000069004Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi78 ↔ 171PROSITE-ProRule annotation
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
Lipidationi311 – 3111S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

PTM databases

PhosphoSiteiQ60614.

Expressioni

Gene expression databases

BgeeiQ60614.
CleanExiMM_ADORA2B.
GenevestigatoriQ60614.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ60614.
SMRiQ60614. Positions 8-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni173 – 18210Agonist bindingBy similarity
Regioni247 – 2548Agonist bindingBy similarity
Regioni266 – 27611Agonist bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG287824.
GeneTreeiENSGT00550000074524.
HOGENOMiHOG000015770.
HOVERGENiHBG106962.
InParanoidiQ60614.
KOiK04267.
OMAiCTDFYGC.
OrthoDBiEOG79SDXQ.
TreeFamiTF325296.

Family and domain databases

InterProiIPR001435. Adeno_A2B_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00554. ADENOSINA2BR.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLETQDALY VALELVIAAL AVAGNVLVCA AVGASSALQT PTNYFLVSLA
60 70 80 90 100
TADVAVGLFA IPFAITISLG FCTDFHGCLF LACFVLVLTQ SSIFSLLAVA
110 120 130 140 150
VDRYLAIRVP LRYKGLVTGT RARGIIAVLW VLAFGIGLTP FLGWNSKDSA
160 170 180 190 200
TSNCTELGDG IANKSCCPVT CLFENVVPMS YMVYFNFFGC VLPPLLIMLV
210 220 230 240 250
IYIKIFMVAC KQLQRMELMD HSRTTLQREI HAAKSLAMIV GIFALCWLPV
260 270 280 290 300
HAINCITLFH PALAKDKPKW VMNVAILLSH ANSVVNPIVY AYRNRDFRYS
310 320 330
FHKIISRYVL CQAETKGGSG QAGAQSTLSL GL
Length:332
Mass (Da):36,120
Last modified:July 27, 2011 - v2
Checksum:iBABE2C782001564A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti169 – 1691V → L in AAA19001 (PubMed:8157966).Curated
Sequence conflicti215 – 2151R → S in AAA19001 (PubMed:8157966).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05673 mRNA. Translation: AAA19001.1.
AK047002 mRNA. Translation: BAC32938.1.
AL596110 Genomic DNA. Translation: CAI35330.1.
BC116415 mRNA. Translation: AAI16416.1.
BC116416 mRNA. Translation: AAI16417.1.
CCDSiCCDS24822.1.
PIRiI48933.
RefSeqiNP_031439.2. NM_007413.4.
UniGeneiMm.40740.

Genome annotation databases

EnsembliENSMUST00000018644; ENSMUSP00000018644; ENSMUSG00000018500.
GeneIDi11541.
KEGGimmu:11541.
UCSCiuc007jio.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05673 mRNA. Translation: AAA19001.1.
AK047002 mRNA. Translation: BAC32938.1.
AL596110 Genomic DNA. Translation: CAI35330.1.
BC116415 mRNA. Translation: AAI16416.1.
BC116416 mRNA. Translation: AAI16417.1.
CCDSiCCDS24822.1.
PIRiI48933.
RefSeqiNP_031439.2. NM_007413.4.
UniGeneiMm.40740.

3D structure databases

ProteinModelPortaliQ60614.
SMRiQ60614. Positions 8-314.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ60614.
ChEMBLiCHEMBL2237.
GuidetoPHARMACOLOGYi20.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ60614.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018644; ENSMUSP00000018644; ENSMUSG00000018500.
GeneIDi11541.
KEGGimmu:11541.
UCSCiuc007jio.1. mouse.

Organism-specific databases

CTDi136.
MGIiMGI:99403. Adora2b.

Phylogenomic databases

eggNOGiNOG287824.
GeneTreeiENSGT00550000074524.
HOGENOMiHOG000015770.
HOVERGENiHBG106962.
InParanoidiQ60614.
KOiK04267.
OMAiCTDFYGC.
OrthoDBiEOG79SDXQ.
TreeFamiTF325296.

Enzyme and pathway databases

ReactomeiREACT_242871. G alpha (s) signalling events.
REACT_253800. Adenosine P1 receptors.

Miscellaneous databases

NextBioi279004.
PROiQ60614.
SOURCEiSearch...

Gene expression databases

BgeeiQ60614.
CleanExiMM_ADORA2B.
GenevestigatoriQ60614.

Family and domain databases

InterProiIPR001435. Adeno_A2B_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00554. ADENOSINA2BR.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of two adenosine receptor subtypes from mouse bone marrow-derived mast cells."
    Marquardt D.L., Walker L.L., Heinemann S.
    J. Immunol. 152:4508-4515(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Bone marrow.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiAA2BR_MOUSE
AccessioniPrimary (citable) accession number: Q60614
Secondary accession number(s): Q8BXI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: January 7, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.