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Q60613

- AA2AR_MOUSE

UniProt

Q60613 - AA2AR_MOUSE

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Protein

Adenosine receptor A2a

Gene

Adora2a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

GO - Molecular functioni

  1. G-protein coupled adenosine receptor activity Source: MGI

GO - Biological processi

  1. adenosine receptor signaling pathway Source: MGI
  2. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
  3. astrocyte activation Source: Ensembl
  4. eating behavior Source: MGI
  5. G-protein coupled receptor signaling pathway Source: MGI
  6. locomotory behavior Source: MGI
  7. negative regulation of alpha-beta T cell activation Source: MGI
  8. negative regulation of cell proliferation Source: Ensembl
  9. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  10. negative regulation of inflammatory response Source: MGI
  11. negative regulation of locomotion Source: Ensembl
  12. negative regulation of neuron apoptotic process Source: Ensembl
  13. negative regulation of protein kinase activity Source: Ensembl
  14. negative regulation of vascular permeability Source: Ensembl
  15. neuron projection morphogenesis Source: Ensembl
  16. positive regulation of acetylcholine secretion, neurotransmission Source: Ensembl
  17. positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway Source: Ensembl
  18. positive regulation of apoptotic signaling pathway Source: MGI
  19. positive regulation of cAMP biosynthetic process Source: MGI
  20. positive regulation of circadian sleep/wake cycle, sleep Source: Ensembl
  21. positive regulation of glutamate secretion Source: Ensembl
  22. positive regulation of protein secretion Source: Ensembl
  23. positive regulation of renal sodium excretion Source: Ensembl
  24. positive regulation of synaptic transmission, GABAergic Source: MGI
  25. positive regulation of synaptic transmission, glutamatergic Source: Ensembl
  26. positive regulation of urine volume Source: Ensembl
  27. prepulse inhibition Source: Ensembl
  28. protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  29. regulation of calcium ion transport Source: Ensembl
  30. regulation of excitatory postsynaptic membrane potential Source: Ensembl
  31. regulation of inhibitory postsynaptic membrane potential Source: MGI
  32. regulation of mitochondrial membrane potential Source: Ensembl
  33. regulation of norepinephrine secretion Source: Ensembl
  34. regulation of synaptic plasticity Source: Ensembl
  35. regulation of transcription, DNA-templated Source: Ensembl
  36. response to amphetamine Source: MGI
  37. response to caffeine Source: Ensembl
  38. response to drug Source: Ensembl
  39. synaptic transmission, cholinergic Source: Ensembl
  40. synaptic transmission, dopaminergic Source: MGI
  41. synaptic transmission, glutamatergic Source: Ensembl
  42. vasodilation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_209347. NGF-independant TRKA activation.
REACT_242871. G alpha (s) signalling events.
REACT_253800. Adenosine P1 receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine receptor A2a
Gene namesi
Name:Adora2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:99402. Adora2a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44ExtracellularBy similarity
Transmembranei5 – 2925Helical; Name=1By similarityAdd
BLAST
Topological domaini30 – 3910CytoplasmicBy similarity
Transmembranei40 – 6324Helical; Name=2By similarityAdd
BLAST
Topological domaini64 – 7411ExtracellularBy similarityAdd
BLAST
Transmembranei75 – 9723Helical; Name=3By similarityAdd
BLAST
Topological domaini98 – 11720CytoplasmicBy similarityAdd
BLAST
Transmembranei118 – 14023Helical; Name=4By similarityAdd
BLAST
Topological domaini141 – 16828ExtracellularBy similarityAdd
BLAST
Transmembranei169 – 19325Helical; Name=5By similarityAdd
BLAST
Topological domaini194 – 22936CytoplasmicBy similarityAdd
BLAST
Transmembranei230 – 25324Helical; Name=6By similarityAdd
BLAST
Topological domaini254 – 2618ExtracellularBy similarity
Transmembranei262 – 28524Helical; Name=7By similarityAdd
BLAST
Topological domaini286 – 410125CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. asymmetric synapse Source: Ensembl
  2. axolemma Source: Ensembl
  3. dendrite Source: Ensembl
  4. endomembrane system Source: Ensembl
  5. integral component of plasma membrane Source: Ensembl
  6. membrane Source: MGI
  7. neuronal cell body Source: Ensembl
  8. plasma membrane Source: MGI
  9. postsynaptic density Source: Ensembl
  10. postsynaptic membrane Source: Ensembl
  11. presynaptic active zone Source: Ensembl
  12. presynaptic membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Adenosine receptor A2aPRO_0000069000Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi68 ↔ 154PROSITE-ProRule annotation
Disulfide bondi71 ↔ 143PROSITE-ProRule annotation
Disulfide bondi74 ↔ 161PROSITE-ProRule annotation
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi254 ↔ 257PROSITE-ProRule annotation

Post-translational modificationi

Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ60613.

PTM databases

PhosphoSiteiQ60613.

Expressioni

Gene expression databases

BgeeiQ60613.
CleanExiMM_ADORA2A.
GenevestigatoriQ60613.

Interactioni

Subunit structurei

Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct. May interact with DRD4.By similarity

Protein-protein interaction databases

IntActiQ60613. 2 interactions.
MINTiMINT-4997181.
STRINGi10090.ENSMUSP00000020395.

Structurei

3D structure databases

ProteinModelPortaliQ60613.
SMRiQ60613. Positions 3-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 17210Agonist bindingBy similarity
Regioni241 – 2488Agonist bindingBy similarity
Regioni259 – 26911Agonist bindingBy similarityAdd
BLAST

Domaini

The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG287824.
GeneTreeiENSGT00550000074524.
HOGENOMiHOG000015770.
HOVERGENiHBG106962.
InParanoidiQ60613.
KOiK04266.
OMAiPGVWANG.
OrthoDBiEOG79SDXQ.
TreeFamiTF325296.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR001513. Adeno_A2A_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00553. ADENOSINA2AR.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60613-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSSVYIMVE LAIAVLAILG NVLVCWAVWI NSNLQNVTNF FVVSLAAADI
60 70 80 90 100
AVGVLAIPFA ITISTGFCAA CHGCLFFACF VLVLTQSSIF SLLAIAIDRY
110 120 130 140 150
IAIRIPLRYN GLVTGMRAKG IIAICWVLSF AIGLTPMLGW NNCSQKDENS
160 170 180 190 200
TKTCGEGRVT CLFEDVVPMN YMVYYNFFAF VLLPLLLMLA IYLRIFLAAR
210 220 230 240 250
RQLKQMESQP LPGERTRSTL QKEVHAAKSL AIIVGLFALC WLPLHIINCF
260 270 280 290 300
TFFCSTCQHA PPWLMYLAII LSHSNSVVNP FIYAYRIREF RQTFRKIIRT
310 320 330 340 350
HVLRRQEPFR AGGSSAWALA AHSTEGEQVS LRLNGHPLGV WANGSAPHSG
360 370 380 390 400
RRPNGYTLGP GGGGSTQGSP GDVELLTQEH QEGQEHPGLG DHLAQGRVGT
410
ASWSSEFAPS
Length:410
Mass (Da):44,971
Last modified:July 27, 2011 - v3
Checksum:iE5670F442D243E71
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771F → I in CAA73787. (PubMed:9262401)Curated
Sequence conflicti77 – 771F → I in CAA73788. (PubMed:9262401)Curated
Sequence conflicti117 – 1171R → K in CAA73787. (PubMed:9262401)Curated
Sequence conflicti117 – 1171R → K in CAA73788. (PubMed:9262401)Curated
Sequence conflicti137 – 1382ML → TA in AAA19000. (PubMed:8157966)Curated
Sequence conflicti146 – 1461K → T in CAA73787. (PubMed:9262401)Curated
Sequence conflicti146 – 1461K → T in CAA73788. (PubMed:9262401)Curated
Sequence conflicti204 – 2041K → P in AAA19000. (PubMed:8157966)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13344, Y13345 Genomic DNA. Translation: CAA73787.1.
Y13346 mRNA. Translation: CAA73788.1.
BC110692 mRNA. Translation: AAI10693.1.
CH466553 Genomic DNA. Translation: EDL31926.1.
CH466553 Genomic DNA. Translation: EDL31927.1.
U05672 mRNA. Translation: AAA19000.1.
CCDSiCCDS23924.1.
PIRiI48932.
RefSeqiNP_033760.2. NM_009630.3.
XP_006513155.1. XM_006513092.1.
XP_006513156.1. XM_006513093.1.
XP_006513157.1. XM_006513094.1.
XP_006513158.1. XM_006513095.1.
UniGeneiMm.333734.

Genome annotation databases

EnsembliENSMUST00000105420; ENSMUSP00000101060; ENSMUSG00000020178.
GeneIDi11540.
KEGGimmu:11540.
UCSCiuc007fqh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13344 , Y13345 Genomic DNA. Translation: CAA73787.1 .
Y13346 mRNA. Translation: CAA73788.1 .
BC110692 mRNA. Translation: AAI10693.1 .
CH466553 Genomic DNA. Translation: EDL31926.1 .
CH466553 Genomic DNA. Translation: EDL31927.1 .
U05672 mRNA. Translation: AAA19000.1 .
CCDSi CCDS23924.1.
PIRi I48932.
RefSeqi NP_033760.2. NM_009630.3.
XP_006513155.1. XM_006513092.1.
XP_006513156.1. XM_006513093.1.
XP_006513157.1. XM_006513094.1.
XP_006513158.1. XM_006513095.1.
UniGenei Mm.333734.

3D structure databases

ProteinModelPortali Q60613.
SMRi Q60613. Positions 3-304.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q60613. 2 interactions.
MINTi MINT-4997181.
STRINGi 10090.ENSMUSP00000020395.

Chemistry

BindingDBi Q60613.
ChEMBLi CHEMBL2115.
GuidetoPHARMACOLOGYi 19.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q60613.

Proteomic databases

PRIDEi Q60613.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000105420 ; ENSMUSP00000101060 ; ENSMUSG00000020178 .
GeneIDi 11540.
KEGGi mmu:11540.
UCSCi uc007fqh.1. mouse.

Organism-specific databases

CTDi 135.
MGIi MGI:99402. Adora2a.

Phylogenomic databases

eggNOGi NOG287824.
GeneTreei ENSGT00550000074524.
HOGENOMi HOG000015770.
HOVERGENi HBG106962.
InParanoidi Q60613.
KOi K04266.
OMAi PGVWANG.
OrthoDBi EOG79SDXQ.
TreeFami TF325296.

Enzyme and pathway databases

Reactomei REACT_209347. NGF-independant TRKA activation.
REACT_242871. G alpha (s) signalling events.
REACT_253800. Adenosine P1 receptors.

Miscellaneous databases

NextBioi 279000.
PROi Q60613.
SOURCEi Search...

Gene expression databases

Bgeei Q60613.
CleanExi MM_ADORA2A.
Genevestigatori Q60613.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR001513. Adeno_A2A_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00553. ADENOSINA2AR.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Aggressiveness, hypoalgesia and high blood pressure in mice lacking the adenosine A2a receptor."
    Ledent C., Vaugeois J.M., Schiffmann S.N., Pedrazzini T., El-Yacoubi M., Vanderhaeghen J.J., Costentin J., Heath J.K., Vassart G., Parmentier M.
    Nature 388:674-678(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/Sv.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Jaw.
  4. "Cloning of two adenosine receptor subtypes from mouse bone marrow-derived mast cells."
    Marquardt D.L., Walker L.L., Heinemann S.
    J. Immunol. 152:4508-4515(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-213.
    Strain: BALB/c.
    Tissue: Bone marrow.

Entry informationi

Entry nameiAA2AR_MOUSE
AccessioniPrimary (citable) accession number: Q60613
Secondary accession number(s): Q2NLC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3