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Q60613 (AA2AR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenosine receptor A2a
Gene names
Name:Adora2a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subunit structure

Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct By similarity. May interact with DRD4 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Domain

The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface By similarity.

Post-translational modification

Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 11404425. Source: MGI

adenosine receptor signaling pathway

Inferred from direct assay PubMed 12223546. Source: MGI

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 10934242PubMed 12223546PubMed 8738226PubMed 9269779. Source: MGI

astrocyte activation

Inferred from electronic annotation. Source: Ensembl

eating behavior

Inferred from genetic interaction PubMed 12538862. Source: MGI

locomotory behavior

Inferred from genetic interaction PubMed 10908627PubMed 11172060PubMed 12538862PubMed 16280580. Source: MGI

negative regulation of alpha-beta T cell activation

Inferred from direct assay PubMed 10069416. Source: MGI

negative regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of inflammatory response

Inferred from genetic interaction PubMed 17601796. Source: MGI

negative regulation of locomotion

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein kinase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of vascular permeability

Inferred from electronic annotation. Source: Ensembl

neuron projection morphogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of acetylcholine secretion, neurotransmission

Inferred from electronic annotation. Source: Ensembl

positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of apoptotic signaling pathway

Inferred from mutant phenotype PubMed 10845921. Source: MGI

positive regulation of cAMP biosynthetic process

Inferred from direct assay PubMed 9269779. Source: MGI

positive regulation of circadian sleep/wake cycle, sleep

Inferred from electronic annotation. Source: Ensembl

positive regulation of glutamate secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of renal sodium excretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of synaptic transmission, GABAergic

Inferred from direct assay PubMed 10934242. Source: MGI

positive regulation of synaptic transmission, glutamatergic

Inferred from electronic annotation. Source: Ensembl

positive regulation of urine volume

Inferred from electronic annotation. Source: Ensembl

prepulse inhibition

Inferred from electronic annotation. Source: Ensembl

protein kinase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

regulation of calcium ion transport

Inferred from electronic annotation. Source: Ensembl

regulation of excitatory postsynaptic membrane potential

Inferred from electronic annotation. Source: Ensembl

regulation of inhibitory postsynaptic membrane potential

Inferred from genetic interaction PubMed 10704492. Source: MGI

regulation of mitochondrial membrane potential

Inferred from electronic annotation. Source: Ensembl

regulation of norepinephrine secretion

Inferred from electronic annotation. Source: Ensembl

regulation of synaptic plasticity

Inferred from electronic annotation. Source: Ensembl

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

response to amphetamine

Inferred from mutant phenotype PubMed 11172060. Source: MGI

response to caffeine

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

synaptic transmission, cholinergic

Inferred from electronic annotation. Source: Ensembl

synaptic transmission, dopaminergic

Inferred from genetic interaction PubMed 12538862. Source: MGI

synaptic transmission, glutamatergic

Inferred from electronic annotation. Source: Ensembl

vasodilation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentasymmetric synapse

Inferred from electronic annotation. Source: Ensembl

axolemma

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from electronic annotation. Source: Ensembl

endomembrane system

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Inferred from electronic annotation. Source: Ensembl

membrane

Inferred from direct assay PubMed 10908627PubMed 8738226. Source: MGI

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay PubMed 12817016. Source: MGI

postsynaptic density

Inferred from electronic annotation. Source: Ensembl

postsynaptic membrane

Inferred from electronic annotation. Source: Ensembl

presynaptic active zone

Inferred from electronic annotation. Source: Ensembl

presynaptic membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionG-protein coupled adenosine receptor activity

Inferred from direct assay PubMed 10908627PubMed 10934242PubMed 12223546PubMed 8738226. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 410410Adenosine receptor A2a
PRO_0000069000

Regions

Topological domain1 – 44Extracellular By similarity
Transmembrane5 – 2925Helical; Name=1; By similarity
Topological domain30 – 3910Cytoplasmic By similarity
Transmembrane40 – 6324Helical; Name=2; By similarity
Topological domain64 – 7411Extracellular By similarity
Transmembrane75 – 9723Helical; Name=3; By similarity
Topological domain98 – 11720Cytoplasmic By similarity
Transmembrane118 – 14023Helical; Name=4; By similarity
Topological domain141 – 16828Extracellular By similarity
Transmembrane169 – 19325Helical; Name=5; By similarity
Topological domain194 – 22936Cytoplasmic By similarity
Transmembrane230 – 25324Helical; Name=6; By similarity
Topological domain254 – 2618Extracellular By similarity
Transmembrane262 – 28524Helical; Name=7; By similarity
Topological domain286 – 410125Cytoplasmic By similarity
Region163 – 17210Agonist binding By similarity
Region241 – 2488Agonist binding By similarity
Region259 – 26911Agonist binding By similarity

Amino acid modifications

Glycosylation1421N-linked (GlcNAc...) Potential
Glycosylation1491N-linked (GlcNAc...) Potential
Disulfide bond68 ↔ 154 By similarity
Disulfide bond71 ↔ 143 By similarity
Disulfide bond74 ↔ 161 By similarity
Disulfide bond254 ↔ 257 By similarity

Experimental info

Sequence conflict771F → I in CAA73787. Ref.1
Sequence conflict771F → I in CAA73788. Ref.1
Sequence conflict1171R → K in CAA73787. Ref.1
Sequence conflict1171R → K in CAA73788. Ref.1
Sequence conflict137 – 1382ML → TA in AAA19000. Ref.4
Sequence conflict1461K → T in CAA73787. Ref.1
Sequence conflict1461K → T in CAA73788. Ref.1
Sequence conflict2041K → P in AAA19000. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q60613 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: E5670F442D243E71

FASTA41044,971
        10         20         30         40         50         60 
MGSSVYIMVE LAIAVLAILG NVLVCWAVWI NSNLQNVTNF FVVSLAAADI AVGVLAIPFA 

        70         80         90        100        110        120 
ITISTGFCAA CHGCLFFACF VLVLTQSSIF SLLAIAIDRY IAIRIPLRYN GLVTGMRAKG 

       130        140        150        160        170        180 
IIAICWVLSF AIGLTPMLGW NNCSQKDENS TKTCGEGRVT CLFEDVVPMN YMVYYNFFAF 

       190        200        210        220        230        240 
VLLPLLLMLA IYLRIFLAAR RQLKQMESQP LPGERTRSTL QKEVHAAKSL AIIVGLFALC 

       250        260        270        280        290        300 
WLPLHIINCF TFFCSTCQHA PPWLMYLAII LSHSNSVVNP FIYAYRIREF RQTFRKIIRT 

       310        320        330        340        350        360 
HVLRRQEPFR AGGSSAWALA AHSTEGEQVS LRLNGHPLGV WANGSAPHSG RRPNGYTLGP 

       370        380        390        400        410 
GGGGSTQGSP GDVELLTQEH QEGQEHPGLG DHLAQGRVGT ASWSSEFAPS 

« Hide

References

« Hide 'large scale' references
[1]"Aggressiveness, hypoalgesia and high blood pressure in mice lacking the adenosine A2a receptor."
Ledent C., Vaugeois J.M., Schiffmann S.N., Pedrazzini T., El-Yacoubi M., Vanderhaeghen J.J., Costentin J., Heath J.K., Vassart G., Parmentier M.
Nature 388:674-678(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: 129/Sv.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Jaw.
[4]"Cloning of two adenosine receptor subtypes from mouse bone marrow-derived mast cells."
Marquardt D.L., Walker L.L., Heinemann S.
J. Immunol. 152:4508-4515(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-213.
Strain: BALB/c.
Tissue: Bone marrow.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y13344, Y13345 Genomic DNA. Translation: CAA73787.1.
Y13346 mRNA. Translation: CAA73788.1.
BC110692 mRNA. Translation: AAI10693.1.
CH466553 Genomic DNA. Translation: EDL31926.1.
CH466553 Genomic DNA. Translation: EDL31927.1.
U05672 mRNA. Translation: AAA19000.1.
CCDSCCDS23924.1.
PIRI48932.
RefSeqNP_033760.2. NM_009630.3.
XP_006513155.1. XM_006513092.1.
XP_006513156.1. XM_006513093.1.
XP_006513157.1. XM_006513094.1.
XP_006513158.1. XM_006513095.1.
UniGeneMm.333734.

3D structure databases

ProteinModelPortalQ60613.
SMRQ60613. Positions 3-304.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ60613. 2 interactions.
MINTMINT-4997181.
STRING10090.ENSMUSP00000020395.

Chemistry

ChEMBLCHEMBL2115.
GuidetoPHARMACOLOGY19.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ60613.

Proteomic databases

PRIDEQ60613.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000105420; ENSMUSP00000101060; ENSMUSG00000020178.
GeneID11540.
KEGGmmu:11540.
UCSCuc007fqh.1. mouse.

Organism-specific databases

CTD135.
MGIMGI:99402. Adora2a.

Phylogenomic databases

eggNOGNOG287824.
GeneTreeENSGT00550000074524.
HOGENOMHOG000015770.
HOVERGENHBG106962.
InParanoidQ2NLC1.
KOK04266.
OMAPGVWANG.
OrthoDBEOG79SDXQ.
TreeFamTF325296.

Gene expression databases

BgeeQ60613.
CleanExMM_ADORA2A.
GenevestigatorQ60613.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001513. Adeno_A2A_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00553. ADENOSINA2AR.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279000.
PROQ60613.
SOURCESearch...

Entry information

Entry nameAA2AR_MOUSE
AccessionPrimary (citable) accession number: Q60613
Secondary accession number(s): Q2NLC1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries