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Q60613

- AA2AR_MOUSE

UniProt

Q60613 - AA2AR_MOUSE

Protein

Adenosine receptor A2a

Gene

Adora2a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

    GO - Molecular functioni

    1. G-protein coupled adenosine receptor activity Source: MGI

    GO - Biological processi

    1. adenosine receptor signaling pathway Source: MGI
    2. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
    3. astrocyte activation Source: Ensembl
    4. eating behavior Source: MGI
    5. G-protein coupled receptor signaling pathway Source: MGI
    6. locomotory behavior Source: MGI
    7. negative regulation of alpha-beta T cell activation Source: MGI
    8. negative regulation of cell proliferation Source: Ensembl
    9. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    10. negative regulation of inflammatory response Source: MGI
    11. negative regulation of locomotion Source: Ensembl
    12. negative regulation of neuron apoptotic process Source: Ensembl
    13. negative regulation of protein kinase activity Source: Ensembl
    14. negative regulation of vascular permeability Source: Ensembl
    15. neuron projection morphogenesis Source: Ensembl
    16. positive regulation of acetylcholine secretion, neurotransmission Source: Ensembl
    17. positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway Source: Ensembl
    18. positive regulation of apoptotic signaling pathway Source: MGI
    19. positive regulation of cAMP biosynthetic process Source: MGI
    20. positive regulation of circadian sleep/wake cycle, sleep Source: Ensembl
    21. positive regulation of glutamate secretion Source: Ensembl
    22. positive regulation of protein secretion Source: Ensembl
    23. positive regulation of renal sodium excretion Source: Ensembl
    24. positive regulation of synaptic transmission, GABAergic Source: MGI
    25. positive regulation of synaptic transmission, glutamatergic Source: Ensembl
    26. positive regulation of urine volume Source: Ensembl
    27. prepulse inhibition Source: Ensembl
    28. protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
    29. regulation of calcium ion transport Source: Ensembl
    30. regulation of excitatory postsynaptic membrane potential Source: Ensembl
    31. regulation of inhibitory postsynaptic membrane potential Source: MGI
    32. regulation of mitochondrial membrane potential Source: Ensembl
    33. regulation of norepinephrine secretion Source: Ensembl
    34. regulation of synaptic plasticity Source: Ensembl
    35. regulation of transcription, DNA-templated Source: Ensembl
    36. response to amphetamine Source: MGI
    37. response to caffeine Source: Ensembl
    38. response to drug Source: Ensembl
    39. synaptic transmission, cholinergic Source: Ensembl
    40. synaptic transmission, dopaminergic Source: MGI
    41. synaptic transmission, glutamatergic Source: Ensembl
    42. vasodilation Source: Ensembl

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_209347. NGF-independant TRKA activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosine receptor A2a
    Gene namesi
    Name:Adora2a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:99402. Adora2a.

    Subcellular locationi

    GO - Cellular componenti

    1. asymmetric synapse Source: Ensembl
    2. axolemma Source: Ensembl
    3. dendrite Source: Ensembl
    4. endomembrane system Source: Ensembl
    5. integral component of plasma membrane Source: Ensembl
    6. membrane Source: MGI
    7. neuronal cell body Source: Ensembl
    8. plasma membrane Source: MGI
    9. postsynaptic density Source: Ensembl
    10. postsynaptic membrane Source: Ensembl
    11. presynaptic active zone Source: Ensembl
    12. presynaptic membrane Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 410410Adenosine receptor A2aPRO_0000069000Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi68 ↔ 154PROSITE-ProRule annotation
    Disulfide bondi71 ↔ 143PROSITE-ProRule annotation
    Disulfide bondi74 ↔ 161PROSITE-ProRule annotation
    Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi254 ↔ 257PROSITE-ProRule annotation

    Post-translational modificationi

    Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ60613.

    PTM databases

    PhosphoSiteiQ60613.

    Expressioni

    Gene expression databases

    BgeeiQ60613.
    CleanExiMM_ADORA2A.
    GenevestigatoriQ60613.

    Interactioni

    Subunit structurei

    Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct. May interact with DRD4.By similarity

    Protein-protein interaction databases

    IntActiQ60613. 2 interactions.
    MINTiMINT-4997181.
    STRINGi10090.ENSMUSP00000020395.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60613.
    SMRiQ60613. Positions 3-304.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 44ExtracellularBy similarity
    Topological domaini30 – 3910CytoplasmicBy similarity
    Topological domaini64 – 7411ExtracellularBy similarityAdd
    BLAST
    Topological domaini98 – 11720CytoplasmicBy similarityAdd
    BLAST
    Topological domaini141 – 16828ExtracellularBy similarityAdd
    BLAST
    Topological domaini194 – 22936CytoplasmicBy similarityAdd
    BLAST
    Topological domaini254 – 2618ExtracellularBy similarity
    Topological domaini286 – 410125CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei5 – 2925Helical; Name=1By similarityAdd
    BLAST
    Transmembranei40 – 6324Helical; Name=2By similarityAdd
    BLAST
    Transmembranei75 – 9723Helical; Name=3By similarityAdd
    BLAST
    Transmembranei118 – 14023Helical; Name=4By similarityAdd
    BLAST
    Transmembranei169 – 19325Helical; Name=5By similarityAdd
    BLAST
    Transmembranei230 – 25324Helical; Name=6By similarityAdd
    BLAST
    Transmembranei262 – 28524Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 17210Agonist bindingBy similarity
    Regioni241 – 2488Agonist bindingBy similarity
    Regioni259 – 26911Agonist bindingBy similarityAdd
    BLAST

    Domaini

    The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.By similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG287824.
    GeneTreeiENSGT00550000074524.
    HOGENOMiHOG000015770.
    HOVERGENiHBG106962.
    InParanoidiQ2NLC1.
    KOiK04266.
    OMAiPGVWANG.
    OrthoDBiEOG79SDXQ.
    TreeFamiTF325296.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR001513. Adeno_A2A_rcpt.
    IPR001634. Adenosn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00553. ADENOSINA2AR.
    PR00424. ADENOSINER.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60613-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSSVYIMVE LAIAVLAILG NVLVCWAVWI NSNLQNVTNF FVVSLAAADI    50
    AVGVLAIPFA ITISTGFCAA CHGCLFFACF VLVLTQSSIF SLLAIAIDRY 100
    IAIRIPLRYN GLVTGMRAKG IIAICWVLSF AIGLTPMLGW NNCSQKDENS 150
    TKTCGEGRVT CLFEDVVPMN YMVYYNFFAF VLLPLLLMLA IYLRIFLAAR 200
    RQLKQMESQP LPGERTRSTL QKEVHAAKSL AIIVGLFALC WLPLHIINCF 250
    TFFCSTCQHA PPWLMYLAII LSHSNSVVNP FIYAYRIREF RQTFRKIIRT 300
    HVLRRQEPFR AGGSSAWALA AHSTEGEQVS LRLNGHPLGV WANGSAPHSG 350
    RRPNGYTLGP GGGGSTQGSP GDVELLTQEH QEGQEHPGLG DHLAQGRVGT 400
    ASWSSEFAPS 410
    Length:410
    Mass (Da):44,971
    Last modified:July 27, 2011 - v3
    Checksum:iE5670F442D243E71
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 771F → I in CAA73787. (PubMed:9262401)Curated
    Sequence conflicti77 – 771F → I in CAA73788. (PubMed:9262401)Curated
    Sequence conflicti117 – 1171R → K in CAA73787. (PubMed:9262401)Curated
    Sequence conflicti117 – 1171R → K in CAA73788. (PubMed:9262401)Curated
    Sequence conflicti137 – 1382ML → TA in AAA19000. (PubMed:8157966)Curated
    Sequence conflicti146 – 1461K → T in CAA73787. (PubMed:9262401)Curated
    Sequence conflicti146 – 1461K → T in CAA73788. (PubMed:9262401)Curated
    Sequence conflicti204 – 2041K → P in AAA19000. (PubMed:8157966)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13344, Y13345 Genomic DNA. Translation: CAA73787.1.
    Y13346 mRNA. Translation: CAA73788.1.
    BC110692 mRNA. Translation: AAI10693.1.
    CH466553 Genomic DNA. Translation: EDL31926.1.
    CH466553 Genomic DNA. Translation: EDL31927.1.
    U05672 mRNA. Translation: AAA19000.1.
    CCDSiCCDS23924.1.
    PIRiI48932.
    RefSeqiNP_033760.2. NM_009630.3.
    XP_006513155.1. XM_006513092.1.
    XP_006513156.1. XM_006513093.1.
    XP_006513157.1. XM_006513094.1.
    XP_006513158.1. XM_006513095.1.
    UniGeneiMm.333734.

    Genome annotation databases

    EnsembliENSMUST00000105420; ENSMUSP00000101060; ENSMUSG00000020178.
    GeneIDi11540.
    KEGGimmu:11540.
    UCSCiuc007fqh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13344 , Y13345 Genomic DNA. Translation: CAA73787.1 .
    Y13346 mRNA. Translation: CAA73788.1 .
    BC110692 mRNA. Translation: AAI10693.1 .
    CH466553 Genomic DNA. Translation: EDL31926.1 .
    CH466553 Genomic DNA. Translation: EDL31927.1 .
    U05672 mRNA. Translation: AAA19000.1 .
    CCDSi CCDS23924.1.
    PIRi I48932.
    RefSeqi NP_033760.2. NM_009630.3.
    XP_006513155.1. XM_006513092.1.
    XP_006513156.1. XM_006513093.1.
    XP_006513157.1. XM_006513094.1.
    XP_006513158.1. XM_006513095.1.
    UniGenei Mm.333734.

    3D structure databases

    ProteinModelPortali Q60613.
    SMRi Q60613. Positions 3-304.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q60613. 2 interactions.
    MINTi MINT-4997181.
    STRINGi 10090.ENSMUSP00000020395.

    Chemistry

    ChEMBLi CHEMBL2115.
    GuidetoPHARMACOLOGYi 19.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q60613.

    Proteomic databases

    PRIDEi Q60613.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000105420 ; ENSMUSP00000101060 ; ENSMUSG00000020178 .
    GeneIDi 11540.
    KEGGi mmu:11540.
    UCSCi uc007fqh.1. mouse.

    Organism-specific databases

    CTDi 135.
    MGIi MGI:99402. Adora2a.

    Phylogenomic databases

    eggNOGi NOG287824.
    GeneTreei ENSGT00550000074524.
    HOGENOMi HOG000015770.
    HOVERGENi HBG106962.
    InParanoidi Q2NLC1.
    KOi K04266.
    OMAi PGVWANG.
    OrthoDBi EOG79SDXQ.
    TreeFami TF325296.

    Enzyme and pathway databases

    Reactomei REACT_209347. NGF-independant TRKA activation.

    Miscellaneous databases

    NextBioi 279000.
    PROi Q60613.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q60613.
    CleanExi MM_ADORA2A.
    Genevestigatori Q60613.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR001513. Adeno_A2A_rcpt.
    IPR001634. Adenosn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00553. ADENOSINA2AR.
    PR00424. ADENOSINER.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Aggressiveness, hypoalgesia and high blood pressure in mice lacking the adenosine A2a receptor."
      Ledent C., Vaugeois J.M., Schiffmann S.N., Pedrazzini T., El-Yacoubi M., Vanderhaeghen J.J., Costentin J., Heath J.K., Vassart G., Parmentier M.
      Nature 388:674-678(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/Sv.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Jaw.
    4. "Cloning of two adenosine receptor subtypes from mouse bone marrow-derived mast cells."
      Marquardt D.L., Walker L.L., Heinemann S.
      J. Immunol. 152:4508-4515(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-213.
      Strain: BALB/c.
      Tissue: Bone marrow.

    Entry informationi

    Entry nameiAA2AR_MOUSE
    AccessioniPrimary (citable) accession number: Q60613
    Secondary accession number(s): Q2NLC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3