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Reviewed, UniProtKB/Swiss-Prot Q60610 (TIAM1_MOUSE)

Last modified June 16, 2009. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    T-lymphoma invasion and metastasis-inducing protein 1
      Short name=TIAM-1
Gene names
Name: Tiam1
Synonyms: Tiam-1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length1591 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA By similarity. Affects invasiveness of T-lymphoma cells.

Subunit structure

Interacts with BAIAP2 By similarity.

Tissue specificity

Highly expressed in brain and testis and at low or moderate levels in almost all other normal tissues. Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas.

Sequence similarities

Belongs to the TIAM family.

Contains 1 DH (DBL-homology) domain.

Contains 1 PDZ (DHR) domain.

Contains 2 PH domains.

Contains 1 RBD (Ras-binding) domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Potential
Chain2 – 15911590T-lymphoma invasion and metastasis-inducing protein 1
PRO_0000080977

Regions

Domain434 – 549116PH 1
Domain765 – 83268RBD
Domain845 – 90864PDZ
Domain1040 – 1234195DH
Domain1261 – 1397137PH 2
Compositional bias595 – 5984Poly-Lys
Compositional bias1445 – 14495Poly-Arg

Amino acid modifications

Modified residue2311Phosphoserine Ref.2
Modified residue13231Phosphotyrosine Ref.3
Lipidation21N-myristoyl glycine Potential

Secondary structure

1 .......................................................... 1591
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q60610-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: ECC2BAB189A6F019

FASTA1,591177,533
        10         20         30         40         50         60 
MGNAESQNVD HEFYGEKHAS LGRKHTSRSL RLSHKTRRTR HASSGKAIHR NSEVSTRSSS 

        70         80         90        100        110        120 
TPSIPQSLAE NGLEPFSQEG ALDDFGDPIW VDRVDMGLRP VSYTDSSVTP SVDGSIVLTA 

       130        140        150        160        170        180 
ASVQSMPDSE ESRLYGDDAT YLAEGGRRQC PYTSNGPTFM ETASFKKKRS KSADIWREDS 

       190        200        210        220        230        240 
LEFSLSDLSQ EHLTSNEEIL GSAEEKDCEE ARGMETEASP RQLSTCQRAN SLGDLYAQKN 

       250        260        270        280        290        300 
SGVKANGGPR NRFSSYCRNL VSDIPDLAKH KMPPAAAEET PPYSNYNTLP CRKSHCLSEG 

       310        320        330        340        350        360 
ATNPQISLSK SMQGRRAKTT QDVNTGEGSE FADSGIEGAT TDTDLLSRRS NATNSSYSPP 

       370        380        390        400        410        420 
TGRAFVGSDS GSSSTGDRAR QGVYENFRRE LEMSTTNSES LEEAGSAHSD EQSSGTLSSP 

       430        440        450        460        470        480 
GQSDILLTAA QGTVRKAGAL AVKNFLVHKK NKKVESATRR KWKHYWVSLK GCTLFFYETD 

       490        500        510        520        530        540 
GRSGIDHNSV PKHAVWVENS IVQAVPEHPK KDFVFCLSNS LGDAFLFQTT SQTELENWIT 

       550        560        570        580        590        600 
AIHSACAAAV ARHHHKEDTL RLLKSEIKKL EQKIDMDEKM KKMGEMQLSS VTDSKKKKTI 

       610        620        630        640        650        660 
LDQIFVWEQN LEQFQMDLFR FRCYLASLQG GELPNPKRLL AFASRPTKVA MGRLGIFSVS 

       670        680        690        700        710        720 
SFHALVAART GEIGVRRRTQ AMSRSASKRR SRFSSLWGLD TTSKKKQGRP TINQVFGEGT 

       730        740        750        760        770        780 
DAVKRSLEGI FDDTVPDGKR EKEVVLPSVH QHNPDCDIWV HEYFTPSWFC LPNNQPALTV 

       790        800        810        820        830        840 
VRPGDTARDT LELICKTHQL DHSAHYLRLK FLMENRVQFY IPQPEEDIYE LLYKEIEICP 

       850        860        870        880        890        900 
KVTQNIHIEK SDAAADNYGF LLSSVDEDGI RRLYVNSVKE TGLASKKGLK AGDEILEINN 

       910        920        930        940        950        960 
RAAGTLNSSM LKDFLSQPSL GLLVRTYPEP EGGVELLENP PHRVDGPVDL GESPLAFLTS 

       970        980        990       1000       1010       1020 
NPGHSLSSEQ GSSAETAPEE GEGPDLESSD ETDHSSKSTE QVAAFCRSLH EMSPSDSSPS 

      1030       1040       1050       1060       1070       1080 
PQDATSPQLA TTRQLSDADK LRKVICELLE TERTYVKDLN CLMERYLKPL QKETFLTQDE 

      1090       1100       1110       1120       1130       1140 
LDVLFGNLTE MVEFQVEFLK TLEDGVRLVP DLEKLEKVDQ FKKVLFSLGG SFLYYADRFK 

      1150       1160       1170       1180       1190       1200 
LYSAFCASHT KVPKVLVKAK TDTAFKAFLD AQNPRQQHSS TLESYLIKPI QRVLKYPLLL 

      1210       1220       1230       1240       1250       1260 
RELFALTDAE SEEHYHLDVA IKTMNKVASH INEMQKIHEE FGAVFDQLIA EQTGEKKEVA 

      1270       1280       1290       1300       1310       1320 
DLSMGDLLLH TSVIWLNPPA SLGKWKKEPE LAAFVFKTAV VLVYKDGSKQ KKKLVGSHRL 

      1330       1340       1350       1360       1370       1380 
SIYEEWDPFR FRHMIPTEAL QVRALPSADA EANAVCEIVH VKSESEGRPE RVFHLCCSSP 

      1390       1400       1410       1420       1430       1440 
ESRKDFLKSV HSILRDKHRR QLLKTESLPS AQQYVPFGGK RLCALKGARP AMSRAVSAPS 

      1450       1460       1470       1480       1490       1500 
KSLGRRRRRL ARNRFTIDSD AISASSPEKE PQQPAGGGDT DRWVEEQFDL AQYEEQDDIK 

      1510       1520       1530       1540       1550       1560 
ETDILSDDDE FCESLKGASV DRDLQEQLQA ASISQRARGR RTLDSHASRM TQLKKQAALS 

      1570       1580       1590 
GINGGLESAS EEVIWVRRED FAPSRKLNTE I

« Hide

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References

« Hide 'large scale' references
[1]"Identification of an invasion-inducing gene, Tiam-1, that encodes a protein with homology to GDP-GTP exchangers for Rho-like proteins."
Habets G.G.M., Scholtes E.H.M., Zuydgeest D., van der Kammen R.A., Stam J.C., Berns A., Collard J.G.
Cell 77:537-549(1994) [PubMed: 7999144] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Brain.
[2]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, MASS SPECTROMETRY.
Tissue: Brain.
[3]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1323, MASS SPECTROMETRY.
Tissue: Mast cell.
[4]"Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiam1."
Worthylake D.K., Rossman K.L., Sondek J.
Nature 408:682-688(2000) [PubMed: 11130063] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 1033-1406 IN COMPLEX WITH RAC1.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U05245 mRNA. Translation: AAA18830.1.
IPIIPI00119006.
PIRA54146.
UniGeneMm.310902

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1FOEX-ray2.80A/C/E/G1033-1406[»]
SMRQ60610. Positions 835-935.
ModBaseSearch...

PTM databases

PhosphoSiteQ60610.

2-D gel databases

SWISS-2DPAGEQ60610.

Proteomic databases

PRIDEQ60610.

Genome annotation databases

EnsemblENSMUSG00000002489. Mus musculus. [Contig view]
KEGGmmu:21844.

Organism-specific databases

MGIMGI:103306. Tiam1.

Phylogenomic databases

HOGENOMQ60610.
HOVERGENQ60610.

Gene expression databases

ArrayExpressQ60610.
BgeeQ60610.
CleanExMM_TIAM1.
GermOnlineENSMUSG00000002489. Mus musculus.

Family and domain databases

InterProIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR001478. PDZ/DHR/GLGF.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR003116. Raf-like_ras_bd.
[Graphical view]
Gene3DG3DSA:2.30.29.30. PH_type. 2 hits.
G3DSA:1.20.900.10. RhoGEF. 1 hit.
PfamPF00595. PDZ. 1 hit.
PF00169. PH. 2 hits.
PF02196. RBD. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
SM00233. PH. 2 hits.
SM00455. RBD. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
PROSITEPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50898. RBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio301314.
PMAP-CutDBQ60610.
SOURCESearch...

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Entry information

Entry nameTIAM1_MOUSE
AccessionPrimary (citable) accession number: Q60610
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents