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Protein

Myosin light polypeptide 6

Gene

Myl6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory light chain of myosin. Does not bind calcium.

GO - Molecular functioni

  • actin-dependent ATPase activity Source: HGNC
  • calcium ion binding Source: InterPro
  • motor activity Source: HGNC
  • structural constituent of muscle Source: HGNC

GO - Biological processi

  • muscle contraction Source: HGNC
  • muscle filament sliding Source: HGNC
  • skeletal muscle tissue development Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Enzyme and pathway databases

ReactomeiR-MMU-445355. Smooth Muscle Contraction.
R-MMU-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light polypeptide 6
Alternative name(s):
17 kDa myosin light chain
Short name:
LC17
Myosin light chain 3
Short name:
MLC-3
Myosin light chain alkali 3
Short name:
Myosin light chain A3
Smooth muscle and nonmuscle myosin light chain alkali 6
Gene namesi
Name:Myl6
Synonyms:Myln
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:109318. Myl6.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: MGI
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • myosin complex Source: HGNC
  • unconventional myosin complex Source: UniProtKB
  • vesicle Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 151150Myosin light polypeptide 6PRO_0000198691Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylcysteine1 Publication
Modified residuei57 – 571PhosphoserineCombined sources
Modified residuei81 – 811N6-acetyllysineCombined sources
Isoform Smooth muscle (identifier: Q60605-2)
Modified residuei135 – 1351PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ60605.
PaxDbiQ60605.
PeptideAtlasiQ60605.
PRIDEiQ60605.
TopDownProteomicsiQ60605-2. [Q60605-2]

2D gel databases

REPRODUCTION-2DPAGEQ60605.
UCD-2DPAGEQ60605.

PTM databases

iPTMnetiQ60605.
PhosphoSiteiQ60605.
SwissPalmiQ60605.

Expressioni

Gene expression databases

BgeeiENSMUSG00000090841.
ExpressionAtlasiQ60605. baseline and differential.
GenevisibleiQ60605. MM.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with SPATA6 (PubMed:25605924).1 Publication

Protein-protein interaction databases

BioGridi201658. 4 interactions.
IntActiQ60605. 9 interactions.
MINTiMINT-4102785.
STRINGi10090.ENSMUSP00000128803.

Structurei

3D structure databases

ProteinModelPortaliQ60605.
SMRiQ60605. Positions 4-151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 4236EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini84 – 11936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini119 – 15133EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0030. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiQ60605.
KOiK12751.
OMAiNHEDANG.
PhylomeDBiQ60605.
TreeFamiTF351553.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Non-muscle (identifier: Q60605-1) [UniParc]FASTAAdd to basket
Also known as: MLC3nm, LC17A, LC17-nm

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCDFTEDQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVLK
60 70 80 90 100
VLGNPKSDEM NVKVLDFEHF LPMLQTVAKN KDQGTYEDYV EGLRVFDKEG
110 120 130 140 150
NGTVMGAEIR HVLVTLGEKM TEEEVEMLVA GHEDSNGCIN YEAFVRHILS

G
Length:151
Mass (Da):16,930
Last modified:January 23, 2007 - v3
Checksum:iA2B7B4F41179523D
GO
Isoform Smooth muscle (identifier: Q60605-2) [UniParc]FASTAAdd to basket
Also known as: MLC3sm, LC17B, LC17-sm

The sequence of this isoform differs from the canonical sequence as follows:
     143-151: AFVRHILSG → ELVRMVLNG

Show »
Length:151
Mass (Da):16,961
Checksum:i25B244E686095233
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541N → T in AAC52278 (PubMed:7804326).Curated
Sequence conflicti82 – 821D → A in AAC52278 (PubMed:7804326).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei143 – 1519AFVRHILSG → ELVRMVLNG in isoform Smooth muscle. 1 PublicationVSP_009736

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026760 mRNA. Translation: AAH26760.1.
U04443 mRNA. Translation: AAC52278.1.
CCDSiCCDS48728.1. [Q60605-2]
RefSeqiNP_001304147.1. NM_001317218.1. [Q60605-1]
NP_034990.1. NM_010860.4. [Q60605-2]
UniGeneiMm.337074.

Genome annotation databases

EnsembliENSMUST00000164181; ENSMUSP00000128803; ENSMUSG00000090841. [Q60605-2]
GeneIDi17904.
KEGGimmu:17904.
UCSCiuc007hnb.1. mouse. [Q60605-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026760 mRNA. Translation: AAH26760.1.
U04443 mRNA. Translation: AAC52278.1.
CCDSiCCDS48728.1. [Q60605-2]
RefSeqiNP_001304147.1. NM_001317218.1. [Q60605-1]
NP_034990.1. NM_010860.4. [Q60605-2]
UniGeneiMm.337074.

3D structure databases

ProteinModelPortaliQ60605.
SMRiQ60605. Positions 4-151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201658. 4 interactions.
IntActiQ60605. 9 interactions.
MINTiMINT-4102785.
STRINGi10090.ENSMUSP00000128803.

PTM databases

iPTMnetiQ60605.
PhosphoSiteiQ60605.
SwissPalmiQ60605.

2D gel databases

REPRODUCTION-2DPAGEQ60605.
UCD-2DPAGEQ60605.

Proteomic databases

MaxQBiQ60605.
PaxDbiQ60605.
PeptideAtlasiQ60605.
PRIDEiQ60605.
TopDownProteomicsiQ60605-2. [Q60605-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000164181; ENSMUSP00000128803; ENSMUSG00000090841. [Q60605-2]
GeneIDi17904.
KEGGimmu:17904.
UCSCiuc007hnb.1. mouse. [Q60605-1]

Organism-specific databases

CTDi4637.
MGIiMGI:109318. Myl6.

Phylogenomic databases

eggNOGiKOG0030. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiQ60605.
KOiK12751.
OMAiNHEDANG.
PhylomeDBiQ60605.
TreeFamiTF351553.

Enzyme and pathway databases

ReactomeiR-MMU-445355. Smooth Muscle Contraction.
R-MMU-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

PROiQ60605.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000090841.
ExpressionAtlasiQ60605. baseline and differential.
GenevisibleiQ60605. MM.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYL6_MOUSE
AccessioniPrimary (citable) accession number: Q60605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.