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Protein

Adseverin

Gene

Scin

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane (PubMed:9671468). Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2) (By similarity). In vitro, also has barbed end capping and nucleating activities in the presence of Ca2+ (PubMed:9671468). Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles (By similarity). Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts (PubMed:25275604, PubMed:25681458). Regulates chondrocyte proliferation and differentiation (By similarity). Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways (By similarity).By similarity3 Publications
Isoform 2: fails to nucleate actin polymerization, although it severs and caps actin filaments in a Ca2+-dependent manner.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi538Calcium 1By similarity1
Metal bindingi539Calcium 1By similarity1
Metal bindingi562Calcium 1By similarity1
Metal bindingi643Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi644Calcium 2By similarity1
Metal bindingi666Calcium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Actin capping

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adseverin
Alternative name(s):
Gelsolin-like protein
Scinderin
Gene namesi
Name:Scin
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1306794. Scin.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: UniProtKB
  • cell junction Source: UniProtKB-KW
  • cell projection Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB-KW
  • extracellular exosome Source: MGI
  • podosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Knockdown in bone marrow monocytes protect mice from bone resorption in periodontal disease model.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187451 – 715AdseverinAdd BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphotyrosineCombined sources1
Modified residuei599PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60604.
PeptideAtlasiQ60604.
PRIDEiQ60604.

PTM databases

iPTMnetiQ60604.
PhosphoSitePlusiQ60604.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in mature osteoclasts (at protein level) (PubMed:25275604). Isoform 2 is expressed in blood cells (PubMed:9671468).2 Publications

Developmental stagei

Expression is induced during osteoclastogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000002565.
CleanExiMM_SCIN.
GenevisibleiQ60604. MM.

Interactioni

Protein-protein interaction databases

BioGridi203095. 1 interactor.
IntActiQ60604. 1 interactor.
MINTiMINT-4087296.
STRINGi10090.ENSMUSP00000002640.

Structurei

3D structure databases

ProteinModelPortaliQ60604.
SMRiQ60604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 76Gelsolin-like 1Add BLAST50
Repeati148 – 188Gelsolin-like 2Add BLAST41
Repeati265 – 307Gelsolin-like 3Add BLAST43
Repeati398 – 451Gelsolin-like 4Add BLAST54
Repeati523 – 564Gelsolin-like 5Add BLAST42
Repeati626 – 668Gelsolin-like 6Add BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 363Actin-severingSequence analysisAdd BLAST363
Regioni112 – 119Polyphosphoinositide bindingBy similarity8
Regioni138 – 146Polyphosphoinositide bindingBy similarity9
Regioni364 – 715Ca(2+)-dependent actin bindingAdd BLAST352

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated
Contains 6 gelsolin-like repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
GeneTreeiENSGT00760000119111.
HOGENOMiHOG000233630.
HOVERGENiHBG004183.
InParanoidiQ60604.
KOiK05768.
OMAiGLYHEEF.
OrthoDBiEOG091G05SC.
TreeFamiTF313468.

Family and domain databases

Gene3Di3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR030012. Adseverin.
IPR007123. Gelsolin-like_dom.
IPR007122. Villin/Gelsolin.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF14. PTHR11977:SF14. 1 hit.
PfamiPF00626. Gelsolin. 6 hits.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
[Graphical view]
SUPFAMiSSF82754. SSF82754. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60604-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQELQHPEF ARAGQQAGLQ VWRVEKLELV PVPQGAYGDF YVGDAYLVLH
60 70 80 90 100
TTKSSRGFSY RLHFWLGKEC SQDESTAAAI FTVQMDDYLG GKPVQSRELQ
110 120 130 140 150
GYESTDFVGY FKGGLKYKAG GVASGLNHVL TNDLTAKRLL HVKGRRVVRA
160 170 180 190 200
TEVPLSWESF NKGDCFIIDL GTEIYQWCGS SCNKYERLKA SQVAIGIRDN
210 220 230 240 250
ERKGRSQLIV VEEGSEPSEL MKVLGRKPEL PDGDNDDDVV ADISNRKMAK
260 270 280 290 300
LYMVSDASGS MKVTLVAEEN PFSMGMLLSE ECFILDHGAA KQIFVWKGKN
310 320 330 340 350
ANPQERKTAM KTAEEFLQKM KYSTNTQIQV LPEGGETPIF KQFFKDWKDK
360 370 380 390 400
DQSDGFGKVY ITEKVAQIKQ IPFDASKLHS SPQMAAQHNM VDDGSGGVEI
410 420 430 440 450
WRVENSGRVQ IDPSSYGEFY GGDCYIILYT YPRGQIIYTW QGANATRDEL
460 470 480 490 500
TMSAFLTVQL DRSLGGQAVQ VRVSQGKEPA HLLSLFKDKP LIIYKNGTSK
510 520 530 540 550
KEGQAPAPPT RLFQVRRNLA SITRIVEVDV DANSLNSNDT FVLKLPRNNG
560 570 580 590 600
FIWIGKGASQ EEEKGAEYVA DVLKCKASRI QEGKEPEEFW NSLGGRGDYQ
610 620 630 640 650
TSPLLETRAE DHPPRLYGCS NKTGRFIIEE VPGEFTQDDL AEDDVMLLDA
660 670 680 690 700
WEQIFIWIGK DANEVEKKES VKSAKMYLET DPSGRDKRTP IVIIKQGHEP
710
PTFTGWFLGW DSSRW
Length:715
Mass (Da):80,254
Last modified:July 27, 2011 - v3
Checksum:i6EF9F45FBC82E249
GO
Isoform 2 (identifier: Q60604-2) [UniParc]FASTAAdd to basket
Also known as: D51 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     528-627: Missing.

Show »
Length:615
Mass (Da):69,110
Checksum:iAF386AFFF4239CDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44 – 45DA → EP in AAB61682 (PubMed:9671468).Curated2
Sequence conflicti44 – 45DA → EP in CAA74304 (PubMed:9671468).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006730528 – 627Missing in isoform 2. CuratedAdd BLAST100

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04354 mRNA. Translation: AAB61682.1.
Y13971 mRNA. Translation: CAA74304.1.
AK133484 mRNA. Translation: BAE21681.1.
BC063328 mRNA. Translation: AAH63328.1.
CCDSiCCDS25891.1. [Q60604-2]
CCDS49055.1. [Q60604-1]
PIRiT10049.
RefSeqiNP_001139668.1. NM_001146196.1. [Q60604-1]
NP_033158.2. NM_009132.2. [Q60604-2]
UniGeneiMm.2416.

Genome annotation databases

EnsembliENSMUST00000002640; ENSMUSP00000002640; ENSMUSG00000002565. [Q60604-1]
ENSMUST00000078481; ENSMUSP00000077573; ENSMUSG00000002565. [Q60604-2]
GeneIDi20259.
KEGGimmu:20259.
UCSCiuc007nkw.2. mouse. [Q60604-1]
uc011yln.1. mouse. [Q60604-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04354 mRNA. Translation: AAB61682.1.
Y13971 mRNA. Translation: CAA74304.1.
AK133484 mRNA. Translation: BAE21681.1.
BC063328 mRNA. Translation: AAH63328.1.
CCDSiCCDS25891.1. [Q60604-2]
CCDS49055.1. [Q60604-1]
PIRiT10049.
RefSeqiNP_001139668.1. NM_001146196.1. [Q60604-1]
NP_033158.2. NM_009132.2. [Q60604-2]
UniGeneiMm.2416.

3D structure databases

ProteinModelPortaliQ60604.
SMRiQ60604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203095. 1 interactor.
IntActiQ60604. 1 interactor.
MINTiMINT-4087296.
STRINGi10090.ENSMUSP00000002640.

PTM databases

iPTMnetiQ60604.
PhosphoSitePlusiQ60604.

Proteomic databases

PaxDbiQ60604.
PeptideAtlasiQ60604.
PRIDEiQ60604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002640; ENSMUSP00000002640; ENSMUSG00000002565. [Q60604-1]
ENSMUST00000078481; ENSMUSP00000077573; ENSMUSG00000002565. [Q60604-2]
GeneIDi20259.
KEGGimmu:20259.
UCSCiuc007nkw.2. mouse. [Q60604-1]
uc011yln.1. mouse. [Q60604-2]

Organism-specific databases

CTDi85477.
MGIiMGI:1306794. Scin.

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
GeneTreeiENSGT00760000119111.
HOGENOMiHOG000233630.
HOVERGENiHBG004183.
InParanoidiQ60604.
KOiK05768.
OMAiGLYHEEF.
OrthoDBiEOG091G05SC.
TreeFamiTF313468.

Miscellaneous databases

PROiQ60604.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002565.
CleanExiMM_SCIN.
GenevisibleiQ60604. MM.

Family and domain databases

Gene3Di3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR030012. Adseverin.
IPR007123. Gelsolin-like_dom.
IPR007122. Villin/Gelsolin.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF14. PTHR11977:SF14. 1 hit.
PfamiPF00626. Gelsolin. 6 hits.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
[Graphical view]
SUPFAMiSSF82754. SSF82754. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADSV_MOUSE
AccessioniPrimary (citable) accession number: Q60604
Secondary accession number(s): O08988, O08990, Q6P4N8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.