Reviewed, UniProtKB/Swiss-Prot Q605W1 (DNAE2_METCA)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	MCA2164

Organism

Methylococcus capsulatus [Complete proteome] [HAMAP]

Taxonomic identifier

414 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length	1047 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1047	1047	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103382

Sequences

	Sequence		Length	Mass (Da)
	Q605W1-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 6CA4ACD601B82A94	FASTA	1,047	116,728
	10 20 30 40 50 60 MACSAEAVFA ELHALSNFSF LRGASHPKEL VAEAARLGYA ALALTDECSL AGVVRAHAEA 70 80 90 100 110 120 KTLGFKLIIG SEFRLEDGPK LVLLAMDRRG YGQLAAFITL GRRAAEKGEY RLLRGQLETH 130 140 150 160 170 180 PLDQCIAIWL PEDAPDDETG AWLARLFPAR CWIGVELFLS GGDGLRLSVL QALGERLGLP 190 200 210 220 230 240 LVACNDVHMH ARERQPLQDT LTAIRLGRPL AELGYALFPN GERHLRPIEF LARIYPRALL 250 260 270 280 290 300 EESLRIAERC RFSLDELRYE YPAELVPDGY TAIAWLRELT RQGMAERWPE GAPDKVRRQV 310 320 330 340 350 360 EHELELIGAM AYEPFFLTVH DVVRFARSRG ILCQGRGSAA NSAVCYCLGI TEVDPARLDL 370 380 390 400 410 420 LFERFISRER NEPPDIDVDF EHERREEVIQ YIYRKYGRHR AALAASLITY RVRSAVRDVA 430 440 450 460 470 480 RALGFSPSRI DTLARVLDRH GVAETLPERL AEAGLASENP AVRRLLALMQ MLVGFPRHLS 490 500 510 520 530 540 QHVGGFVIAA EDLSHWVPVE NAAMPERTVI QWDKDDLESL GLLKVDVLSL GMLTAIRKAL 550 560 570 580 590 600 AYVGEGRGRP FTLADVPPED PAVYEMLQRA DSIGVFQVES RAQMSMLPRL RPQSYYDLVI 610 620 630 640 650 660 QIAIVRPGPI QGDMVHPYLT RRAGLEPVSY PSPAVEKVLK RTLGVPIFQE QVMQLAMVAA 670 680 690 700 710 720 GFTPGEADRL RRAMAAWHRK GGLEPFEKKL MDGMRERGYE ERFARQIYRQ IRGFGEYGFP 730 740 750 760 770 780 ESHSASFALL AYVSAWLKCH HPAAFACALL NSQPMGFYGP SQLIQDARRH GVEVRPIDVN 790 800 810 820 830 840 HSDWDCTLEP TEIPNPLSLW ERVGVRALQT GSNPALRLGL RLVNGFSSAA AQRLSDARRQ 850 860 870 880 890 900 GPFQSIQDLA TRVPLDRREL EALAAADALH GLGGHRHRAF WEAAGVEAPT PLYAEPQFAE 910 920 930 940 950 960 AEPLLRKPGE AEDVIADYAA AGASLRRHPL SLLRERLDRR GFRTAEALWQ VRNGAIARVA 970 980 990 1000 1010 1020 GLVVCRQRPM TANGTTFVTI EDETGQINLI VWPATAQAQR RALLRAHLLA VSGTVQQEEG 1030 1040 VLHLVAGRLE DIGKWLDGLV VKSRDFQ

References

[1]

"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)." Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. , Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A. PLoS Biol. 2:1616-1628(2004) [PubMed: 15383840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bath / NCIMB 11132.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017282 Genomic DNA. Translation: AAU91620.1.
RefSeq	YP_114587.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3104948.
GenomeReviews	Gene locus MCA2164 in contig AE017282_GR.
KEGG	mca:MCA2164.
NMPDR	fig|243233.4.peg.2112.
TIGR	MCA2164.
Phylogenomic databases
HOGENOM	Q605W1.
OMA	LATNRNG.
Enzyme and pathway databases
BioCyc	MCAP243233:MCA_2164-MON.
BRENDA	2.7.7.7. 2172.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_METCA

Accession

Primary (citable) accession number: Q605W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)