Q605Q9 (FTHS_METCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	MCA2219

Organism

Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]

Taxonomic identifier

243233 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000199360
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q605Q9 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: F0655EC3CF7A38E8	FASTA	557	59,411
	10 20 30 40 50 60 MSDIEIAQRA KMLPIIDLAR EKLGIPAASL DPYGHYKAKV ALDYIDGLKD RPDGKLILVT 70 80 90 100 110 120 AISPTPAGEG KTTTTVGLGD ALNRIGKKTV MCLREPSLGP CFGVKGGAAG GGHAQVVPME 130 140 150 160 170 180 DINLHFTGDF HAVGVAHNLL SALIDNHINH GNALDIDPRR IQWKRVVDMN DRALRKIVVG 190 200 210 220 230 240 MGGTANGYLR EDGFDIVVAS EVMAILCLAT SMADLKERLG RIIVGYKSDG KTPVYARDLK 250 260 270 280 290 300 AHGAMAALLK DAIKPNLVQT LENNLAIIHG GPFANIAHGC NTVTATQTAL KLADYVVTEA 310 320 330 340 350 360 GFGADLGAEK FIDIKCRMAG LNPAAVVLVA TVRALKFHGG VKKEDLNQEN LAALEAGFAN 370 380 390 400 410 420 LERHVHNIRE HYGLPCVVSI NHFSFDTEAE IAWLMKKCEA LGVKAVLARH WAEGGKGAEA 430 440 450 460 470 480 LARTVADIVD HQPGRHTFVY GDEATLWNKI ETIATKIYGA AGISADAKVK AQLEAWNADY 490 500 510 520 530 540 GHYPVCMAKT QMSFSTDPNA KGAPSGHTVA IREVRLANGA GFVVAIAGDM MTMPGLPKVP 550 AAEHIDVDDD GRISGLF

References

[1]

"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)." Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. , Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A. PLoS Biol. 2:1616-1628(2004) [PubMed: 15383840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33009 / NCIMB 11132 / Bath.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017282 Genomic DNA. Translation: AAU91814.1.
RefSeq	YP_114639.1. NC_002977.6.
3D structure databases
ProteinModelPortal	Q605Q9.
SMR	Q605Q9. Positions 3-555.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3102111.
GenomeReviews	Gene locus MCA2219 in contig AE017282_GR.
KEGG	mca:MCA2219.
NMPDR	fig|243233.4.peg.2164.
PATRIC	22608288. VBIMetCap22254_2248.
TIGR	MCA2219.
Phylogenomic databases
HOGENOM	HBG677721.
OMA	TDFTVTI.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	MCAP243233:MCA_2219-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_METCA

Accession

Primary (citable) accession number: Q605Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program