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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei563Aminoacyl-adenylateUniRule annotation1
Binding sitei607ATPUniRule annotation1
Metal bindingi902ZincUniRule annotation1
Metal bindingi905ZincUniRule annotation1
Metal bindingi922ZincUniRule annotation1
Metal bindingi925ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMCAP243233:G1G0X-2221-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MCA2253
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
Proteomesi
  • UP000006821 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984151 – 939Isoleucine--tRNA ligaseAdd BLAST939

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243233.MCA2253

Structurei

3D structure databases

ProteinModelPortaliQ605M7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi604 – 608"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q605M7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKSTLNLP QTPFPMKANL AQREPEQIER WRTLDLYRKV RSRRAGRDKF
60 70 80 90 100
ILHDGPPYAN GEIHIGHAVN KILKDFIVKS RTLSGFDAPY VPGWDCHGLP
110 120 130 140 150
IELMVEKKIG KAGHKVSPAE FRKACREYAA AQVDLQRREF IRLGVLGDWE
160 170 180 190 200
NPYLTMDFRF EADIVRALGR IAARGHLAKG AKPVHWCLDC GSALAEAEVE
210 220 230 240 250
YENKHSPAID VRFAVVDEFG LMARFHTADG ALGEGPLSVV IWTTTPWTLP
260 270 280 290 300
ANQAVALNPE LDYVVVQRTD IKERLVLADA LMKDVMLRCG AEGYRVIGYC
310 320 330 340 350
KGAALEGVQL RHPFYDRIVP VILGDHVTLD AGTGAVHTAP GHGQEDYAVG
360 370 380 390 400
QRYGLAVDNP VGGDGRFLPN TELFAGEHVL SANDHVIEVL KERGALLHEA
410 420 430 440 450
RIEHSYPHCW RHKTPVIFRA TPQWFIAMDK GELRRQALEA IGQVQWIPDW
460 470 480 490 500
GQARIEAMVG NRPDWCISRQ RTWGVPIPLF VHKETGALHP DTDRLIEEIA
510 520 530 540 550
KKIETAGIDA WFELDPAELL GAEADRYGKI GDTLDVWFDS GVTHHAVLAQ
560 570 580 590 600
NPDLAFPADL YLEGSDQHRG WFQSSLMTSV AMSGQAPYKA VLTHGFTVDA
610 620 630 640 650
EGKKMSKSRG NVVAPQKVMQ TLGADVLRLW VAATDYRGEM TVSDEILKRI
660 670 680 690 700
SDVYRRIRNT ARYLLANLDG FDPALHLVKP EDMLALDRWV VDRALAIQQE
710 720 730 740 750
VIEAYETYQF NTIFQKTHHF CSVDLGSFYL DVLKDRQYTC KTDSLPRRSG
760 770 780 790 800
QTAMYHIAEA MVRWLAPVLS FTAEEIWQYL PGQREESVFL SEWYDGLFGL
810 820 830 840 850
DENSRCDRAF WDQMLKIREA VSKELEILRV RGEIGASLDA EVELFCDPPL
860 870 880 890 900
FRKLSEAGDE LRFVLLTSYA TVKPAAEAGT DALPTEIPGL ELKLAASGKP
910 920 930
KCVRCWHHRH DVGTHPDHPE LCGRCVDNVA GTGELRTFG
Length:939
Mass (Da):105,504
Last modified:November 23, 2004 - v1
Checksum:i2A212FE9025A9D6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA Translation: AAU91752.1
RefSeqiWP_010961483.1, NC_002977.6

Genome annotation databases

EnsemblBacteriaiAAU91752; AAU91752; MCA2253
KEGGimca:MCA2253

Similar proteinsi

Entry informationi

Entry nameiSYI_METCA
AccessioniPrimary (citable) accession number: Q605M7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health