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Q605M7

- SYI_METCA

UniProt

Q605M7 - SYI_METCA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, MCA2253
Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylate By similarity
Binding sitei607 – 6071ATP By similarity
Metal bindingi902 – 9021Zinc By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi922 – 9221Zinc By similarity
Metal bindingi925 – 9251Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:MCA2253
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
ProteomesiUP000006821: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098415Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243233.MCA2253.

Structurei

3D structure databases

ProteinModelPortaliQ605M7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi604 – 6085"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q605M7-1 [UniParc]FASTAAdd to Basket

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MDYKSTLNLP QTPFPMKANL AQREPEQIER WRTLDLYRKV RSRRAGRDKF    50
ILHDGPPYAN GEIHIGHAVN KILKDFIVKS RTLSGFDAPY VPGWDCHGLP 100
IELMVEKKIG KAGHKVSPAE FRKACREYAA AQVDLQRREF IRLGVLGDWE 150
NPYLTMDFRF EADIVRALGR IAARGHLAKG AKPVHWCLDC GSALAEAEVE 200
YENKHSPAID VRFAVVDEFG LMARFHTADG ALGEGPLSVV IWTTTPWTLP 250
ANQAVALNPE LDYVVVQRTD IKERLVLADA LMKDVMLRCG AEGYRVIGYC 300
KGAALEGVQL RHPFYDRIVP VILGDHVTLD AGTGAVHTAP GHGQEDYAVG 350
QRYGLAVDNP VGGDGRFLPN TELFAGEHVL SANDHVIEVL KERGALLHEA 400
RIEHSYPHCW RHKTPVIFRA TPQWFIAMDK GELRRQALEA IGQVQWIPDW 450
GQARIEAMVG NRPDWCISRQ RTWGVPIPLF VHKETGALHP DTDRLIEEIA 500
KKIETAGIDA WFELDPAELL GAEADRYGKI GDTLDVWFDS GVTHHAVLAQ 550
NPDLAFPADL YLEGSDQHRG WFQSSLMTSV AMSGQAPYKA VLTHGFTVDA 600
EGKKMSKSRG NVVAPQKVMQ TLGADVLRLW VAATDYRGEM TVSDEILKRI 650
SDVYRRIRNT ARYLLANLDG FDPALHLVKP EDMLALDRWV VDRALAIQQE 700
VIEAYETYQF NTIFQKTHHF CSVDLGSFYL DVLKDRQYTC KTDSLPRRSG 750
QTAMYHIAEA MVRWLAPVLS FTAEEIWQYL PGQREESVFL SEWYDGLFGL 800
DENSRCDRAF WDQMLKIREA VSKELEILRV RGEIGASLDA EVELFCDPPL 850
FRKLSEAGDE LRFVLLTSYA TVKPAAEAGT DALPTEIPGL ELKLAASGKP 900
KCVRCWHHRH DVGTHPDHPE LCGRCVDNVA GTGELRTFG 939
Length:939
Mass (Da):105,504
Last modified:November 23, 2004 - v1
Checksum:i2A212FE9025A9D6C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017282 Genomic DNA. Translation: AAU91752.1.
RefSeqiYP_114671.1. NC_002977.6.

Genome annotation databases

EnsemblBacteriaiAAU91752; AAU91752; MCA2253.
GeneIDi3103165.
KEGGimca:MCA2253.
PATRICi22608354. VBIMetCap22254_2281.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017282 Genomic DNA. Translation: AAU91752.1 .
RefSeqi YP_114671.1. NC_002977.6.

3D structure databases

ProteinModelPortali Q605M7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243233.MCA2253.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU91752 ; AAU91752 ; MCA2253 .
GeneIDi 3103165.
KEGGi mca:MCA2253.
PATRICi 22608354. VBIMetCap22254_2281.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33009 / NCIMB 11132 / Bath.

Entry informationi

Entry nameiSYI_METCA
AccessioniPrimary (citable) accession number: Q605M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi