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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • glutamine biosynthetic process Source: JCVI

Keywordsi

Molecular functionMultifunctional enzyme, Nucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMCAP243233:G1G0X-2317-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:MCA2345
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
Proteomesi
  • UP000006821 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092531 – 948Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST948

Proteomic databases

PRIDEiQ605D9

Interactioni

Protein-protein interaction databases

STRINGi243233.MCA2345

Structurei

3D structure databases

ProteinModelPortaliQ605D9
SMRiQ605D9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 445Adenylyl removaseUniRule annotationAdd BLAST445
Regioni451 – 948Adenylyl transferaseUniRule annotationAdd BLAST498

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6 Bacteria
COG1391 LUCA
HOGENOMiHOG000256491
KOiK00982
OMAiFTVQLLQ
OrthoDBiPOG091H0675

Family and domain databases

HAMAPiMF_00802 GlnE, 1 hit
InterProiView protein in InterPro
IPR023057 GlnE
IPR005190 GlnE_rpt_dom
IPR013546 PII_UdlTrfase/GS_AdlTrfase
PANTHERiPTHR30621 PTHR30621, 1 hit
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 2 hits
PF03710 GlnE, 2 hits

Sequencei

Sequence statusi: Complete.

Q605D9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPPDTSGS CSAFHECERI FAQWGYPFQD YSESFRQSLD TVCRCSRFFL
60 70 80 90 100
GVVKRDAHVI PLLASLFEGQ TPERNPSADL QQRVAEVADR NGLMRVLRQW
110 120 130 140 150
RNREMAIIAW QDICGLLDID SVLTRISETA ESAIRTALDW LFDDACRRWG
160 170 180 190 200
VPRRKDGSAQ SLVVLGMGKL GGYELNFSSD IDLVFAYVED GELPGRNGAT
210 220 230 240 250
YAEFYTRLAQ SLVHVLDAVT EDGFVFRVDV RLRPFGESGP LVVSFDSCER
260 270 280 290 300
YYQAQARDWE RYAMVKVRAV GGDAKDGREF ERFFHPFVYR RYLDYRVFGE
310 320 330 340 350
LRSLKARIMA ELRRKDRGDN IKLGAGGIRE IEFIGQAFQL IRGGRNVELQ
360 370 380 390 400
DRRILVVLER LGRLRLLEHG TASFLCAAYR FLRKVENRLQ QYEDKQTHEL
410 420 430 440 450
PVSEERRGLL AFSMGFESWE GFLHRLDEVR RRVHEIFTEV IAEPPQPARG
460 470 480 490 500
EPLLDGGEAE LSQALAGFDG AAEAMTGALV KFRASAPVRR LGAGARAELQ
510 520 530 540 550
RVIGKLLAEL AAFRVSDPVG VLGRILELFE SIAGRGVYFS LLAENPSALS
560 570 580 590 600
QLVRLAAASP WIVRLIAGAP ILLDELLDPR TLYSPLTRES LGREADILFS
610 620 630 640 650
SLAPDDDEQL MLRLRQFKAA HQLRIAAADI MNVIPVMVVS DYLTDLAEVV
660 670 680 690 700
IERVLRLAWK FAAARHGIPP VEAETSVDFP GFGIIAYGKL GGIELGYGSD
710 720 730 740 750
LDLVFLYDGV ASDAVTDGPR PISAAEFYAR VVQRMVSLLT ADMPGGALYE
760 770 780 790 800
VDLRLRPSGS SGLLVSKADA YENYQLHQAW TWERQALVRA RFVAGDPKVG
810 820 830 840 850
ARFDAIRRNV LCRERDSRQV RLDVRSMREK MREHLADRRP GVFDLKQGVG
860 870 880 890 900
GIADIEFIVQ FGVLISAAKH CEITRWTDTV RLLDSLRAIN FLELGQADRL
910 920 930 940
RCAYCDYRGR VHRLALQEMP ALAASSEFPG HRTAVEAVWK QIIEAPVF
Length:948
Mass (Da):106,527
Last modified:November 23, 2004 - v1
Checksum:i0EB7140FB6C9A957
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA Translation: AAU91502.1
RefSeqiWP_010961573.1, NC_002977.6

Genome annotation databases

EnsemblBacteriaiAAU91502; AAU91502; MCA2345
KEGGimca:MCA2345

Similar proteinsi

Entry informationi

Entry nameiGLNE_METCA
AccessioniPrimary (citable) accession number: Q605D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 23, 2004
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health