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Q60592 (MAST2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Microtubule-associated serine/threonine-protein kinase 2

EC=2.7.11.1
Gene names
Name:Mast2
Synonyms:Mast205
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1734 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1

Cofactor

Magnesium. Ref.1

Subunit structure

Interacts with CDHR2 By similarity. Ref.5 Ref.7

Subcellular location

Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Cell junction. Note: Colocalizes with beta 2-syntrophin and utrophin at neuromuscular junctions. Ref.5

Tissue specificity

Detected in round spermatids and residual bodies but not epididymal spermatozoa (at protein level). Expressed in adult but not fetal testis with levels increasing in parallel with testicular development. Also expressed at high levels in heart, lower levels in all other tissues tested. Ref.1 Ref.4 Ref.5 Ref.7

Post-translational modification

Phosphorylated and ubiquitinated. N-terminal ubiquitination leads to degradation of MAST2 by proteasome-mediated proteolysis. N-terminal phosphorylation appears to be a prerequisite for ubiquitination. Ref.6 Ref.7

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PDZ (DHR) domain.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PTENP604844EBI-493888,EBI-696162From a different organism.
UBBP0CG472EBI-493888,EBI-413034From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17341734Microtubule-associated serine/threonine-protein kinase 2
PRO_0000086313

Regions

Domain453 – 726274Protein kinase
Domain727 – 79569AGC-kinase C-terminal
Domain1042 – 113089PDZ
Nucleotide binding459 – 4679ATP By similarity UniProtKB Q9BXM7

Sites

Active site5761Proton acceptor By similarity UniProtKB Q9BXM7
Binding site4821ATP By similarity UniProtKB Q9BXM7

Amino acid modifications

Modified residue141Phosphoserine By similarity
Modified residue881Phosphoserine By similarity
Modified residue1491Phosphoserine By similarity
Modified residue8411Phosphoserine By similarity
Modified residue9701Phosphoserine By similarity
Modified residue12751Phosphoserine Ref.8
Modified residue14461Phosphothreonine By similarity

Experimental info

Mutagenesis482 – 4832KK → RA: Abolishes LPS-stimulated IL12B synthesis. Ref.6
Sequence conflict1621R → RSHGHRTD in AAH60703. Ref.2
Sequence conflict2321R → A in AAH60703. Ref.2
Sequence conflict275 – 2784PSLT → LAD in AAH60703. Ref.2
Sequence conflict4781R → C Ref.3
Sequence conflict12281Missing Ref.2
Sequence conflict12281Missing Ref.3
Sequence conflict15021T → S Ref.3
Sequence conflict15211V → L Ref.2
Sequence conflict15211V → L Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q60592 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 97292FACD85F12E3

FASTA1,734190,534
        10         20         30         40         50         60 
MVTGLSPLLF RKLSNPDIFA PTGKVKLQRQ LSQDDCKLRR GSLASSLSGK QLLPLSSSVH 

        70         80         90        100        110        120 
SSVGQVTWQS TGEASNLVRM RNQSLGQSAP SLTAGLKELS LPRRGSFCRT SNRKSLIVTS 

       130        140        150        160        170        180 
STSPTLPRPH SPLHGHTGNS PLDSPRNFSP NAPAHFSFVP ARRTDGRRWS LASLPSSGYG 

       190        200        210        220        230        240 
TNTPSSTVSS SCSSQEKLHQ LPFQPTADEL HFLTKHFSTE NVPDEEGRRS PRMRPRSRSL 

       250        260        270        280        290        300 
SPGRSPVSFD SEIIMMNHVY KERFPKATAQ MEERPSLTFI SSNTPDSVLP LADGALSFIH 

       310        320        330        340        350        360 
HQVIEMARDC LDKSRSGLIT SHYFYELQEN LEKLLQDAHE RSESSDVAFV IQLVKKLMII 

       370        380        390        400        410        420 
IARPARLLEC LEFDPEEFYH LLEAAEGHAK EGHGIKCDIP RYIVSQLGLT RDPLEEMAQL 

       430        440        450        460        470        480 
SSYDSPDTPE TDDSVEGRGV SQPSQKTPSE EDFETIKLIS NGAYGAVFLV RHKSTRQRFA 

       490        500        510        520        530        540 
MKKINKQNLI LRNQIQQAFV ERDILTFAEN PFVVSMFCSF ETKRHLCMVM EYVEGGDCAT 

       550        560        570        580        590        600 
LLKNIGALPV DMVRLYFAET VLALEYLHNY GIVHRDLKPD NLLITSMGHI KLTDFGLSKI 

       610        620        630        640        650        660 
GLMSLTTNLY EGHIEKDARE FLDKQVCGTP EYIAPEVILR QGYGKPVDWW AMGIILYEFL 

       670        680        690        700        710        720 
VGCVPFFGDT PEELFGQVIS DEIVWPEGDD ALPPDAQDLT SKLLHQNPLE RLGTSSAYEV 

       730        740        750        760        770        780 
KQHPFFMGLD WTGLLRQKAE FIPQLESEDD TSYFDTRSER YHHVDSEDEE EVSEDGCLEI 

       790        800        810        820        830        840 
RQFSSCSPRF SKVYSSMERL SLLEERRTPP PTKRSLSEEK EDHSDGLAGL KGRDRSWVIG 

       850        860        870        880        890        900 
SPEILRKRLS VSESSHTESD SSPPMTVRHR CSGLPDGPHC PEETSSTPRK QQQEGIWVLI 

       910        920        930        940        950        960 
PPSGEGSSRP VPERPLERQL KLDEEPPGQS SRCCPALETR GRGTPQLAEE ATAKAISDLA 

       970        980        990       1000       1010       1020 
VRRARHRLLS GDSIEKRTTR PVNKVIKSAS ATALSLLIPS EHHACSPLAS PMSPHSQSSN 

      1030       1040       1050       1060       1070       1080 
PSSRDSSPSR DFLPALGSLR PPIIIHRAGK KYGFTLRAIR VYMGDTDVYT VHHMVWHVED 

      1090       1100       1110       1120       1130       1140 
GGPASEAGLR QGDLITHVNG EPVHGLVHTE VVELVLKSGN KVSISTTPLE NTSIKVGPAR 

      1150       1160       1170       1180       1190       1200 
KGSYKAKMAR RSKRSKGKDG QESRKRSSLF RKITKQASLL HTSRSLSSLN RSLSSGESGP 

      1210       1220       1230       1240       1250       1260 
GSPTHSHSLS PRSPPQGYRV APDAVHSVGG NSSQSSSPSS SVPSSPAGSG HTRPSSLHGL 

      1270       1280       1290       1300       1310       1320 
APKLQRQYRS PRRKSAGSIP LSPLAHTPSP PATAASPQRS PSPLSGHGSQ SFPTKLHLSP 

      1330       1340       1350       1360       1370       1380 
PLGRQLSRPK SAEPPRSPLL KRVQSAEKLA AALAAAEKKL APSRKHSLDL PHGELKKELT 

      1390       1400       1410       1420       1430       1440 
PREASPLEVV GTRSVLSGKG PLPGKGVLQP APSRALGTLR QDRAERRESL QKQEAIREVD 

      1450       1460       1470       1480       1490       1500 
SSEDDTDEEP ENSQATQEPR LSPHPEASHN LLPKGSGEGT EEDTFLHRDL KKQGPVLSGL 

      1510       1520       1530       1540       1550       1560 
VTGATLGSPR VDVPGLSPRK VSRPQAFEEA TNPLQVPSLS RSGPTSPTPS EGCWKAQHLH 

      1570       1580       1590       1600       1610       1620 
TQALTALCPS FSELTPTGCS AATSTSGKPG TWSWKFLIEG PDRASTNKTI TRKGEPANSQ 

      1630       1640       1650       1660       1670       1680 
DTNTTVPNLL KNLSPEEEKP QPPSVPGLTH PLLEVPSQNW PWESECEQME KEEPSLSITE 

      1690       1700       1710       1720       1730 
VPDSSGDRRQ DIPCRAHPLS PETRPSLLWK SQELGGQQDH QDLALTSDEL LKQT 

« Hide

References

« Hide 'large scale' references
[1]"A novel 205-kDa testis-specific serine/threonine protein kinase associated with microtubules of the spermatid manchette."
Walden P.D., Cowan N.J.
Mol. Cell. Biol. 13:7625-7635(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: Swiss Webster.
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 302-1734.
Tissue: Brain.
[4]"Increased activity associated with the MAST205 protein kinase complex during mammalian spermiogenesis."
Walden P.D., Millette C.F.
Biol. Reprod. 55:1039-1044(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]"Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases."
Lumeng C., Phelps S., Crawford G.E., Walden P.D., Barald K., Chamberlain J.S.
Nat. Neurosci. 2:611-617(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SNTB2.
[6]"Microtubule-associated serine/threonine kinase-205 kDa and Fc gamma receptor control IL-12 p40 synthesis and NF-kappa B activation."
Zhou H., Xiong H., Li H., Plevy S.E., Walden P.D., Sassaroli M., Prestwich G.D., Unkeless J.C.
J. Immunol. 172:2559-2568(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, UBIQUITINATION, MUTAGENESIS OF 482-LYS-LYS-483.
[7]"Interaction of TRAF6 with MAST205 regulates NF-kappaB activation and MAST205 stability."
Xiong H., Li H., Chen Y., Zhao J., Unkeless J.C.
J. Biol. Chem. 279:43675-43683(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, UBIQUITINATION, PHOSPHORYLATION, INTERACTION WITH TRAF6.
[8]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U02313 mRNA. Translation: AAC04312.1.
BC060703 mRNA. Translation: AAH60703.1.
AB093264 mRNA. Translation: BAC41448.1.
PIRA54602.
RefSeqNP_001036208.1. NM_001042743.2.
NP_032667.2. NM_008641.3.
UniGeneMm.9287.

3D structure databases

ProteinModelPortalQ60592.
SMRQ60592. Positions 263-788, 1037-1131.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201600. 1 interaction.
IntActQ60592. 7 interactions.
MINTMINT-152278.
STRING10090.ENSMUSP00000102095.

PTM databases

PhosphoSiteQ60592.

Proteomic databases

PaxDbQ60592.
PRIDEQ60592.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID17776.
KEGGmmu:17776.

Organism-specific databases

CTD23139.
MGIMGI:894676. Mast2.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000046662.
HOVERGENHBG052414.
KOK08789.

Gene expression databases

CleanExMM_MAST2.
GenevestigatorQ60592.

Family and domain databases

Gene3D1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR028779. MAST2.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24356:SF116. PTHR24356:SF116. 1 hit.
PfamPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMAST2. mouse.
NextBio292503.
PROQ60592.
SOURCESearch...

Entry information

Entry nameMAST2_MOUSE
AccessionPrimary (citable) accession number: Q60592
Secondary accession number(s): Q6P9M1, Q8CHD1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot