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Q60561

- RIR2_MESAU

UniProt

Q60561 - RIR2_MESAU

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Protein

Ribonucleoside-diphosphate reductase subunit M2

Gene
RRM2
Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity. Inhibits Wnt signaling By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi139 – 1391Iron 1 By similarity
Metal bindingi170 – 1701Iron 1 By similarity
Metal bindingi170 – 1701Iron 2 By similarity
Metal bindingi173 – 1731Iron 1 By similarity
Active sitei177 – 1771 By similarity
Metal bindingi233 – 2331Iron 2 By similarity
Metal bindingi267 – 2671Iron 2 By similarity
Metal bindingi270 – 2701Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. deoxyribonucleotide biosynthetic process Source: UniProtKB
  3. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit M2 (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small chain
Ribonucleotide reductase small subunit
Gene namesi
Name:RRM2
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Ribonucleoside-diphosphate reductase subunit M2PRO_0000190449Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201Phosphoserine By similarity
Modified residuei33 – 331Phosphothreonine By similarity

Post-translational modificationi

Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling By similarity.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Structurei

3D structure databases

ProteinModelPortaliQ60561.
SMRiQ60561. Positions 7-353.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG001647.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60561-1 [UniParc]FASTAAdd to Basket

« Hide

MFSVRVPLAT ITDQQQLQVS PLKALSLADK ENTPPSLSAT PVLASKVARR    50
ILQDVAEPES KVSTNPSVED EPLLRENPRR FVVFPIEYHD IWKMYKKAEA 100
SFWTAEEVDL SKDIQHWEAL KPDERHFISH VLAFFAASDG IVNENLVERF 150
SQEVQVTEAR CFYGFQIAME NIHSEMYSLL IDTYIKDSKE REYLFNAIET 200
MPCVKKKADW ALRWIGDKEA TYGERVVAFA AVEGIFFSGS FASIFWLKKR 250
GLMPGLTFSN ELISRDEGLH CDFACLMFKH LVHKPSEQRV QEIITNAVRI 300
EQEFLTEALP VKLIGMNCTL MKQYIEFVAD RLMLELGFNK IFKVENPFDF 350
MENISLEGKT NFFEKRVGEY QRMGVMSNSF TLDADF 386
Length:386
Mass (Da):44,482
Last modified:November 1, 1997 - v1
Checksum:i8B057993D859F9A6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68127 mRNA. Translation: CAA48232.1.
PIRiS27153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68127 mRNA. Translation: CAA48232.1 .
PIRi S27153.

3D structure databases

ProteinModelPortali Q60561.
SMRi Q60561. Positions 7-353.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG001647.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA sequence of the small subunit of the hamster ribonucleotide reductase."
    Chaudhuri M.M., Tonin P.N., Srinivasan P.R.
    Biochim. Biophys. Acta 1171:117-121(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiRIR2_MESAU
AccessioniPrimary (citable) accession number: Q60561
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: December 11, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two distinct regulatory sites have been defined: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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