Reviewed, UniProtKB/Swiss-Prot Q60555 (3BHS1_MESAU)

Names and origin

Protein names

Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
      Short name=3-beta-HSD I
Including the following 2 domains:
    1- Recommended name:
            3-beta-hydroxy-Delta(5)-steroid dehydrogenase
              EC=1.1.1.145
        Alternative name(s):
            3-beta-hydroxy-5-ene steroid dehydrogenase
            Progesterone reductase
    2- Recommended name:
            Steroid Delta-isomerase
              EC=5.3.3.1
        Alternative name(s):
            Delta-5-3-ketosteroid isomerase

Gene names

Name:

HSD3B1

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes hormonal steroids. Also able to act as 3-keto-reductase reducing 5-alpha-dihydrotestosterone to the corresponding 3-beta-androstan-3-beta,17-beta-diol.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue specificity	High levels in adrenal, kidney and male liver. Low levels in female liver.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 373	372	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1		PRO_0000087777
Regions
	Transmembrane	288 – 308	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q60555-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4975BC9F2D93D159	FASTA	373	41,754
	10 20 30 40 50 60 MPGWSCLVTG AGGFLGQRII RMLVQEKELQ EVRALDKVFR PETREEFCKL QTKTKVTVLE 70 80 90 100 110 120 GDILDAQCLR RACQGISVVI HTAAAIDVFG AIPRQTIIDI NLKGTLNLLE ACVQASVPAF 130 140 150 160 170 180 IYTSSIDVAG PNSYKEIVLN GHEEQQHEST WSDPYPYSKK MAEKAVLAAN GSSLKNGGTL 190 200 210 220 230 240 HTCALRPMYI YGEKSPLISV TIIRAVKNSG ILDVTGKFST VNPVYVNNAA WAHILAARGL 250 260 270 280 290 300 QDPRKSPNIQ GQFYYISDDT PHQSYDDLNY VLSKDWGLRP DSSWRPPVAL LYWLGFLLEL 310 320 330 340 350 360 VSFLLRPVYN YQPPFNRHLV TLSNTVFTFS YKKAQRDLGY EPLVGWEEAR ENTSEWIGSL 370 VEQHKGTLNT KAQ

References

[1]

"Expression and characterization of isoforms of 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase in the hamster." Rogerson F.M., Lehoux J.-G., Mason J.I. J. Steroid Biochem. Mol. Biol. 55:481-487(1995) [PubMed: 8547173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L38709 mRNA. Translation: AAB52546.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q60555.
Family and domain databases
InterPro	IPR002225. 3Beta_OHSteriod_DHase/Estase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3BHS1_MESAU

Accession

Primary (citable) accession number: Q60555
Secondary accession number(s): Q60556

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)