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Protein

Homeobox protein Nkx-6.1

Gene

NKX6-1

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity). Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene.By similarity4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi236 – 29560HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • detection of glucose Source: BHF-UCL
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • pancreas development Source: BHF-UCL
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of type B pancreatic cell development Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW
  • type B pancreatic cell maturation Source: BHF-UCL
  • type B pancreatic cell proliferation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-6.1
Alternative name(s):
Homeobox protein NK-6 homolog A
Gene namesi
Name:NKX6-1
Synonyms:NKX6A
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi321 – 3244EEDD → RPPR: Loss of DNA -binding specificity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Homeobox protein Nkx-6.1PRO_0000048952Add
BLAST

Expressioni

Tissue specificityi

Pancreatic beta cells.

Structurei

3D structure databases

ProteinModelPortaliQ60554.
SMRiQ60554. Positions 243-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni101 – 268168Repressor domainAdd
BLAST
Regioni306 – 36459Involved in DNA-bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi49 – 6012Poly-SerAdd
BLAST
Compositional biasi118 – 13114Poly-SerAdd
BLAST
Compositional biasi135 – 15016Poly-AlaAdd
BLAST
Compositional biasi168 – 1736Poly-Pro

Domaini

The C-terminal domain contributes to sequence-specific DNA-binding.By similarity

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG070987.
OrthoDBiEOG091G142D.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
IPR033630. NKX-6.1.
[Graphical view]
PANTHERiPTHR24340:SF31. PTHR24340:SF31. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAVGAMEGP RQSAFLLSSP PLAALHSMAE MKTPLYPATY PPLPTGPPSS
60 70 80 90 100
SSSSSSSSPS PPLGAHNPGG LKPPAAGGLS SLSSPPQQLS AATPHGINDI
110 120 130 140 150
LSRPSMPVAS GAALPSASPS GSSSSSSSSA SATSASAAAA AAAAAAAAAA
160 170 180 190 200
SSPAGLLAGL PRFSSLSPPP PPPGLYFSPS AAAVAAVGRY PKPLAELPGR
210 220 230 240 250
APIFWPGVMQ SPPWRDARLA CTPHQGSILL DKDGKRKHTR PTFSGQQIFA
260 270 280 290 300
LEKTFEQTKY LAGPERARLA YSLGMTESQV KVWFQNRRTK WRKKHAAEMA
310 320 330 340 350
TAKKKQDSET ERLKGTSENE EEDDDYNKPL DPNSDDEKIT QLLKKHKSSS
360
GGLLLHASEA EGSS
Length:364
Mass (Da):37,646
Last modified:November 1, 1996 - v1
Checksum:i08B1AE1225F0F06E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81409 mRNA. Translation: CAA57166.1.
PIRiI48188.
RefSeqiNP_001268625.1. NM_001281696.1.

Genome annotation databases

GeneIDi101826537.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81409 mRNA. Translation: CAA57166.1.
PIRiI48188.
RefSeqiNP_001268625.1. NM_001281696.1.

3D structure databases

ProteinModelPortaliQ60554.
SMRiQ60554. Positions 243-293.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101826537.

Organism-specific databases

CTDi4825.

Phylogenomic databases

HOVERGENiHBG070987.
OrthoDBiEOG091G142D.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
IPR033630. NKX-6.1.
[Graphical view]
PANTHERiPTHR24340:SF31. PTHR24340:SF31. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNKX61_MESAU
AccessioniPrimary (citable) accession number: Q60554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.