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Q60544

- TAF1_MESAU

UniProt

Q60544 - TAF1_MESAU

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Protein
Transcription initiation factor TFIID subunit 1
Gene
TAF1, CCG1
Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4 By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Cofactori

Magnesium By similarity.

Enzyme regulationi

Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1. Binding to TAF1 or CIITA inhibits the histone acetyltransferase activity By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi1190 – 126879HMG box By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. histone acetyltransferase activity Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. DNA-templated transcription, initiation Source: InterPro
  2. histone acetylation Source: GOC
  3. mitotic G1 phase Source: BHF-UCL
  4. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  5. protein phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 1 (EC:2.3.1.48, EC:2.7.11.1)
Alternative name(s):
Cell cycle gene 1 protein
TBP-associated factor 250 kDa
Short name:
p250
Transcription initiation factor TFIID 250 kDa subunit
Short name:
TAF(II)250
Short name:
TAFII-250
Short name:
TAFII250
Gene namesi
Name:TAF1
Synonyms:CCG1
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. MLL1 complex Source: UniProtKB
  2. transcription factor TFIID complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18651865Transcription initiation factor TFIID subunit 1
PRO_0000278523Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei131 – 1311Phosphoserine; by autocatalysis By similarity
Modified residuei302 – 3021Phosphoserine; by autocatalysis By similarity
Modified residuei539 – 5391N6-acetyllysine By similarity
Disulfide bondi1359 ↔ 1614 By similarity
Modified residuei1821 – 18211Phosphoserine By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PRIDEiQ60544.

Interactioni

Subunit structurei

TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H1.4, H2A, H2B, H3 and H4 (in vitro) By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ60544.
SMRiQ60544. Positions 1354-1620.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 409409Protein kinase 1
Add
BLAST
Domaini1392 – 146271Bromo 1
Add
BLAST
Domaini1420 – 1865446Protein kinase 2
Add
BLAST
Domaini1515 – 158571Bromo 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni512 – 971460Histone acetyltransferase (HAT) By similarity
Add
BLAST
Regioni1337 – 1624288Interaction with ASF1A and ASF1B By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1346 – 13538Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi152 – 1609Poly-Pro
Compositional biasi178 – 1858Poly-Ser
Compositional biasi293 – 2975Poly-Pro
Compositional biasi1822 – 18298Poly-Glu

Sequence similaritiesi

Belongs to the TAF1 family.
Contains 2 bromo domains.

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

HOVERGENiHBG050223.

Family and domain databases

Gene3Di1.10.1100.10. 1 hit.
1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR011177. TAF1_animal.
IPR009067. TAF_II_230-bd.
IPR022591. TFIID_sub1_DUF3591.
[Graphical view]
PfamiPF00439. Bromodomain. 2 hits.
PF12157. DUF3591. 1 hit.
PF09247. TBP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF003047. TAF1_animal. 1 hit.
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47055. SSF47055. 1 hit.
SSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60544-1 [UniParc]FASTAAdd to Basket

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MLLPATGASR SAAIMSDTDS DEDSSGGGPF SLTGFLFGNI NGAGQLEGES     50
VLDDECKKHL AGLGALGLGS LITELTANEE LAGTDGALVN DEGWIRSRED 100
AVDYSDINEV AEDESRRYQQ TMGSLQPLCH SADYDEDDYD ADCEDIDCKL 150
MPPPPPPPGP VKKEKDQDGL TGEKVDFSSS SDSESEMGPQ EAAQAESKDG 200
KLTLPLAGIM QHDATKLLPS VTELFPEFRP GKVLRFLRLF GPGKNVPSVW 250
RSARRKRKKK HREPIQEEQI QEEECSVELE VNQKSLWNYD YAPPPPPEQC 300
LSDDEITMMA PVESKFSQST GDTDKVMDTK PRVAEWRYGP ARLWYDMLGV 350
PEDGSGFDYG FKMRKTEHEP AIKCKMMTKL RKLEESNGID LLADENFLMV 400
TQLHWEDDII WDGEDVKHKG TKPQRASLAG WLPSSMTRNA MAYNVQQGFA 450
ATLDDDKPWY SIFPIDNEDL VYGRWEDNII WDAQAMPRIL EPPVLTLDPN 500
DENLILEIPD EKEEATSNSP SKENKKESSL KKSRILLGKT GVIKEEPQQN 550
MSQPEVKDPW NLSNDEYYYP KQQGLRGTFG GNIIQHSIPA VELRQPFFPT 600
HMGPIKLRQF HRPPLKKYSF GALSQPGPHS VQPLLKHIKK KAKMREQERQ 650
ASGGGEMFFM RTPQDLTGKD GDLILAEYSE ENGPLMMQVG MATKIKNYYK 700
RKPGKDPGAP DCKYGETVYC HTSPFLGSLH PGQLLQAFEN NLFRAPIYLH 750
KMPETDFLII RTRQGYYIRE LVDIFVVGQQ CPLFEVPGPN SKRANTHIRD 800
FLQVFIYRLF WKSKDRPRRI RMEDIKKAFP SHSESSIRKR LKLCADFKRT 850
GMDSNWWVLK SDFRLPTEEE IRAMVSPEQC CAYYSMIAAE QRLKDAGYGE 900
KSFFAPEEEN EEDFQMKIDD EVRTAPWNTT RAFIAAMKGK CLLEVTGVAD 950
PTGCGEGFSY VKIPNKPTQQ KDDKEPQPVK KTVTGTDADL RRLSLKNAKQ 1000
LLRKFGVPEE EIKKLSRWEV IDVVRTMSTE QARSGEGPMS KFARGSRFSV 1050
AEHQERYKEE CQRIFDLQNK VLSSTEVLST DTDSSSAEDS DFEEMGKNIE 1100
NMLQNKKTSS QLSREREEQE RKELQRMLLA AGSASAGNNH RDDDTASVTS 1150
LNSSATGRCL KIYRTFRDEE GKEYVRCETV RKPAVIDAYV RIRTTKDEEF 1200
IRKFALFDEQ HREEMRKERR RIQEQLRRLK RNQEKEKLKG PPEKKPKKMK 1250
ERPDLKLKCG ACGAIGHMRT NKFCPLYYQT NAPPSNPVAM TEEQEEELEK 1300
TVIHNDNEEL IKVEGTKIVL GKQLIESADE VRRKSLVLKF PKQQLPPKKK 1350
RRVGTTVHCD YLNRPHKSIH RRRTDPMVTL SSILESIIND MRDLPNTYPF 1400
HTPVNAKVVK DYYKIITRPM DLQTLRENVR KRLYPSREEF REHLELIVKN 1450
SATYNGPKHS LTQISQSMLD LCDEKLKEKE DKLARLEKAI NPLLDDDDQV 1500
AFSFILDNIV TQKMMAVPDS WPFHHPVNKK FVPDYYKVIV SPMDLETIRK 1550
NISKHKYQSR ESFLDDVNLI LANSVKYNGS ESQYTKTAQE IVNVCYQTLT 1600
EYDEHLTQLE KDICTAKEAA LEEAELESLD PMTPGPYTPQ PPDLYDNSTS 1650
LSVSRDASVY QDESNMSVLD IPSATSEKQL TQEGEDGDGD LADEEEGTVQ 1700
QPQASVLYED LLMSEGEDDE EDAGSDEEGD NPFSAIQLSE SGSDSDVGSG 1750
SIRPKQPRVL QENTRMGMEN EESMMSYEGD GGEVSRGLED SNISYGSYEE 1800
PDPKSNTQDT SFSSIGGYEV SEEEEDEEEQ RSGPSVLSQV HLSEDEEDSE 1850
DFHSIAGDSD MDSDE 1865
Length:1,865
Mass (Da):211,868
Last modified:November 1, 1996 - v1
Checksum:iA81614946C0C0F24
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26114 mRNA. Translation: BAA05110.1.
PIRiI48155.
RefSeqiNP_001268498.1. NM_001281569.1.

Genome annotation databases

GeneIDi101838159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26114 mRNA. Translation: BAA05110.1 .
PIRi I48155.
RefSeqi NP_001268498.1. NM_001281569.1.

3D structure databases

ProteinModelPortali Q60544.
SMRi Q60544. Positions 1354-1620.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q60544.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101838159.

Organism-specific databases

CTDi 6872.

Phylogenomic databases

HOVERGENi HBG050223.

Family and domain databases

Gene3Di 1.10.1100.10. 1 hit.
1.20.920.10. 2 hits.
InterProi IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR011177. TAF1_animal.
IPR009067. TAF_II_230-bd.
IPR022591. TFIID_sub1_DUF3591.
[Graphical view ]
Pfami PF00439. Bromodomain. 2 hits.
PF12157. DUF3591. 1 hit.
PF09247. TBP-binding. 1 hit.
[Graphical view ]
PIRSFi PIRSF003047. TAF1_animal. 1 hit.
PRINTSi PR00503. BROMODOMAIN.
SMARTi SM00297. BROMO. 2 hits.
[Graphical view ]
SUPFAMi SSF47055. SSF47055. 1 hit.
SSF47370. SSF47370. 2 hits.
PROSITEi PS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster."
    Hayashida T., Sekiguchi T., Noguchi E., Sunamoto H., Ohba T., Nishimoto T.
    Gene 141:267-270(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTAF1_MESAU
AccessioniPrimary (citable) accession number: Q60544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi