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Protein

Transcription initiation factor TFIID subunit 1

Gene

TAF1

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity (By similarity). Essential for progression of the G1 phase of the cell cycle (PubMed:8163200). Exhibits histone acetyltransferase activity towards histones H3 and H4 (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Cofactori

Mg2+By similarity

Enzyme regulationi

Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1. Binding to TAF1 or CIITA inhibits the histone acetyltransferase activity.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1190 – 1268HMG boxBy similarityAdd BLAST79

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA-templated transcription, initiation Source: InterPro
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 1 (EC:2.3.1.48, EC:2.7.11.1)
Alternative name(s):
Cell cycle gene 1 protein
TBP-associated factor 250 kDa
Short name:
p250
Transcription initiation factor TFIID 250 kDa subunit
Short name:
TAF(II)250
Short name:
TAFII-250
Short name:
TAFII2501 Publication
Gene namesi
Name:TAF1
Synonyms:CCG1
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002785231 – 1865Transcription initiation factor TFIID subunit 1Add BLAST1865

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei131Phosphoserine; by autocatalysisBy similarity1
Modified residuei302Phosphoserine; by autocatalysisBy similarity1
Modified residuei539N6-acetyllysineBy similarity1
Modified residuei1664PhosphoserineBy similarity1
Modified residuei1667PhosphoserineBy similarity1
Modified residuei1773PhosphoserineBy similarity1
Modified residuei1776PhosphoserineBy similarity1
Modified residuei1794PhosphoserineBy similarity1
Modified residuei1821PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by casein kinase II in vitro.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ60544.

Interactioni

Subunit structurei

TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Interacts with TAF7; the interaction is direct. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H1.4, H2A, H2B, H3 and H4 (in vitro).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ60544.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 409Protein kinase 1Add BLAST409
Domaini1392 – 1462Bromo 1PROSITE-ProRule annotationAdd BLAST71
Domaini1420 – 1865Protein kinase 2Add BLAST446
Domaini1515 – 1585Bromo 2PROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni512 – 971Histone acetyltransferase (HAT)By similarityAdd BLAST460
Regioni1337 – 1624Interaction with ASF1A and ASF1BBy similarityAdd BLAST288

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1346 – 1353Nuclear localization signalSequence analysis8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi152 – 160Poly-Pro9
Compositional biasi178 – 185Poly-Ser8
Compositional biasi293 – 297Poly-Pro5
Compositional biasi1822 – 1829Poly-Glu8

Sequence similaritiesi

Belongs to the TAF1 family.Curated
Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 1 HMG box DNA-binding domain.Curated
Contains 2 protein kinase domains.Curated

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

HOVERGENiHBG050223.
OrthoDBiEOG091G00MO.

Family and domain databases

Gene3Di1.10.1100.10. 1 hit.
1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR011177. TAF1_animal.
IPR009067. TAF_II_230-bd.
IPR022591. TFIID_sub1_DUF3591.
[Graphical view]
PfamiPF00439. Bromodomain. 2 hits.
PF12157. DUF3591. 1 hit.
PF09247. TBP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF003047. TAF1_animal. 1 hit.
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47055. SSF47055. 1 hit.
SSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60544-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLPATGASR SAAIMSDTDS DEDSSGGGPF SLTGFLFGNI NGAGQLEGES
60 70 80 90 100
VLDDECKKHL AGLGALGLGS LITELTANEE LAGTDGALVN DEGWIRSRED
110 120 130 140 150
AVDYSDINEV AEDESRRYQQ TMGSLQPLCH SADYDEDDYD ADCEDIDCKL
160 170 180 190 200
MPPPPPPPGP VKKEKDQDGL TGEKVDFSSS SDSESEMGPQ EAAQAESKDG
210 220 230 240 250
KLTLPLAGIM QHDATKLLPS VTELFPEFRP GKVLRFLRLF GPGKNVPSVW
260 270 280 290 300
RSARRKRKKK HREPIQEEQI QEEECSVELE VNQKSLWNYD YAPPPPPEQC
310 320 330 340 350
LSDDEITMMA PVESKFSQST GDTDKVMDTK PRVAEWRYGP ARLWYDMLGV
360 370 380 390 400
PEDGSGFDYG FKMRKTEHEP AIKCKMMTKL RKLEESNGID LLADENFLMV
410 420 430 440 450
TQLHWEDDII WDGEDVKHKG TKPQRASLAG WLPSSMTRNA MAYNVQQGFA
460 470 480 490 500
ATLDDDKPWY SIFPIDNEDL VYGRWEDNII WDAQAMPRIL EPPVLTLDPN
510 520 530 540 550
DENLILEIPD EKEEATSNSP SKENKKESSL KKSRILLGKT GVIKEEPQQN
560 570 580 590 600
MSQPEVKDPW NLSNDEYYYP KQQGLRGTFG GNIIQHSIPA VELRQPFFPT
610 620 630 640 650
HMGPIKLRQF HRPPLKKYSF GALSQPGPHS VQPLLKHIKK KAKMREQERQ
660 670 680 690 700
ASGGGEMFFM RTPQDLTGKD GDLILAEYSE ENGPLMMQVG MATKIKNYYK
710 720 730 740 750
RKPGKDPGAP DCKYGETVYC HTSPFLGSLH PGQLLQAFEN NLFRAPIYLH
760 770 780 790 800
KMPETDFLII RTRQGYYIRE LVDIFVVGQQ CPLFEVPGPN SKRANTHIRD
810 820 830 840 850
FLQVFIYRLF WKSKDRPRRI RMEDIKKAFP SHSESSIRKR LKLCADFKRT
860 870 880 890 900
GMDSNWWVLK SDFRLPTEEE IRAMVSPEQC CAYYSMIAAE QRLKDAGYGE
910 920 930 940 950
KSFFAPEEEN EEDFQMKIDD EVRTAPWNTT RAFIAAMKGK CLLEVTGVAD
960 970 980 990 1000
PTGCGEGFSY VKIPNKPTQQ KDDKEPQPVK KTVTGTDADL RRLSLKNAKQ
1010 1020 1030 1040 1050
LLRKFGVPEE EIKKLSRWEV IDVVRTMSTE QARSGEGPMS KFARGSRFSV
1060 1070 1080 1090 1100
AEHQERYKEE CQRIFDLQNK VLSSTEVLST DTDSSSAEDS DFEEMGKNIE
1110 1120 1130 1140 1150
NMLQNKKTSS QLSREREEQE RKELQRMLLA AGSASAGNNH RDDDTASVTS
1160 1170 1180 1190 1200
LNSSATGRCL KIYRTFRDEE GKEYVRCETV RKPAVIDAYV RIRTTKDEEF
1210 1220 1230 1240 1250
IRKFALFDEQ HREEMRKERR RIQEQLRRLK RNQEKEKLKG PPEKKPKKMK
1260 1270 1280 1290 1300
ERPDLKLKCG ACGAIGHMRT NKFCPLYYQT NAPPSNPVAM TEEQEEELEK
1310 1320 1330 1340 1350
TVIHNDNEEL IKVEGTKIVL GKQLIESADE VRRKSLVLKF PKQQLPPKKK
1360 1370 1380 1390 1400
RRVGTTVHCD YLNRPHKSIH RRRTDPMVTL SSILESIIND MRDLPNTYPF
1410 1420 1430 1440 1450
HTPVNAKVVK DYYKIITRPM DLQTLRENVR KRLYPSREEF REHLELIVKN
1460 1470 1480 1490 1500
SATYNGPKHS LTQISQSMLD LCDEKLKEKE DKLARLEKAI NPLLDDDDQV
1510 1520 1530 1540 1550
AFSFILDNIV TQKMMAVPDS WPFHHPVNKK FVPDYYKVIV SPMDLETIRK
1560 1570 1580 1590 1600
NISKHKYQSR ESFLDDVNLI LANSVKYNGS ESQYTKTAQE IVNVCYQTLT
1610 1620 1630 1640 1650
EYDEHLTQLE KDICTAKEAA LEEAELESLD PMTPGPYTPQ PPDLYDNSTS
1660 1670 1680 1690 1700
LSVSRDASVY QDESNMSVLD IPSATSEKQL TQEGEDGDGD LADEEEGTVQ
1710 1720 1730 1740 1750
QPQASVLYED LLMSEGEDDE EDAGSDEEGD NPFSAIQLSE SGSDSDVGSG
1760 1770 1780 1790 1800
SIRPKQPRVL QENTRMGMEN EESMMSYEGD GGEVSRGLED SNISYGSYEE
1810 1820 1830 1840 1850
PDPKSNTQDT SFSSIGGYEV SEEEEDEEEQ RSGPSVLSQV HLSEDEEDSE
1860
DFHSIAGDSD MDSDE
Length:1,865
Mass (Da):211,868
Last modified:November 1, 1996 - v1
Checksum:iA81614946C0C0F24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26114 mRNA. Translation: BAA05110.1.
PIRiI48155.
RefSeqiNP_001268498.1. NM_001281569.1.

Genome annotation databases

GeneIDi101838159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26114 mRNA. Translation: BAA05110.1.
PIRiI48155.
RefSeqiNP_001268498.1. NM_001281569.1.

3D structure databases

ProteinModelPortaliQ60544.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ60544.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101838159.

Organism-specific databases

CTDi6872.

Phylogenomic databases

HOVERGENiHBG050223.
OrthoDBiEOG091G00MO.

Family and domain databases

Gene3Di1.10.1100.10. 1 hit.
1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR011177. TAF1_animal.
IPR009067. TAF_II_230-bd.
IPR022591. TFIID_sub1_DUF3591.
[Graphical view]
PfamiPF00439. Bromodomain. 2 hits.
PF12157. DUF3591. 1 hit.
PF09247. TBP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF003047. TAF1_animal. 1 hit.
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47055. SSF47055. 1 hit.
SSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAF1_MESAU
AccessioniPrimary (citable) accession number: Q60544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.