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Q60544

- TAF1_MESAU

UniProt

Q60544 - TAF1_MESAU

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Protein

Transcription initiation factor TFIID subunit 1

Gene

TAF1

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4 (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Cofactori

Magnesium.By similarity

Enzyme regulationi

Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1. Binding to TAF1 or CIITA inhibits the histone acetyltransferase activity (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi1190 – 126879HMG boxBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. histone acetyltransferase activity Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. DNA-templated transcription, initiation Source: InterPro
  3. histone acetylation Source: GOC
  4. mitotic G1 phase Source: BHF-UCL
  5. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  6. protein phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 1 (EC:2.3.1.48, EC:2.7.11.1)
Alternative name(s):
Cell cycle gene 1 protein
TBP-associated factor 250 kDa
Short name:
p250
Transcription initiation factor TFIID 250 kDa subunit
Short name:
TAF(II)250
Short name:
TAFII-250
Short name:
TAFII250
Gene namesi
Name:TAF1
Synonyms:CCG1
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. MLL1 complex Source: UniProtKB
  2. transcription factor TFIID complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18651865Transcription initiation factor TFIID subunit 1PRO_0000278523Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei131 – 1311Phosphoserine; by autocatalysisBy similarity
Modified residuei302 – 3021Phosphoserine; by autocatalysisBy similarity
Modified residuei539 – 5391N6-acetyllysineBy similarity
Disulfide bondi1359 ↔ 1614By similarity
Modified residuei1821 – 18211PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PRIDEiQ60544.

Interactioni

Subunit structurei

TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H1.4, H2A, H2B, H3 and H4 (in vitro) (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ60544.
SMRiQ60544. Positions 1354-1620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 409409Protein kinase 1Add
BLAST
Domaini1392 – 146271Bromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini1420 – 1865446Protein kinase 2Add
BLAST
Domaini1515 – 158571Bromo 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni512 – 971460Histone acetyltransferase (HAT)By similarityAdd
BLAST
Regioni1337 – 1624288Interaction with ASF1A and ASF1BBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1346 – 13538Nuclear localization signalSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi152 – 1609Poly-Pro
Compositional biasi178 – 1858Poly-Ser
Compositional biasi293 – 2975Poly-Pro
Compositional biasi1822 – 18298Poly-Glu

Sequence similaritiesi

Belongs to the TAF1 family.Curated
Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 1 HMG box DNA-binding domain.Curated
Contains 2 protein kinase domains.Curated

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

HOVERGENiHBG050223.

Family and domain databases

Gene3Di1.10.1100.10. 1 hit.
1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR011177. TAF1_animal.
IPR009067. TAF_II_230-bd.
IPR022591. TFIID_sub1_DUF3591.
[Graphical view]
PfamiPF00439. Bromodomain. 2 hits.
PF12157. DUF3591. 1 hit.
PF09247. TBP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF003047. TAF1_animal. 1 hit.
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47055. SSF47055. 1 hit.
SSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60544 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLPATGASR SAAIMSDTDS DEDSSGGGPF SLTGFLFGNI NGAGQLEGES
60 70 80 90 100
VLDDECKKHL AGLGALGLGS LITELTANEE LAGTDGALVN DEGWIRSRED
110 120 130 140 150
AVDYSDINEV AEDESRRYQQ TMGSLQPLCH SADYDEDDYD ADCEDIDCKL
160 170 180 190 200
MPPPPPPPGP VKKEKDQDGL TGEKVDFSSS SDSESEMGPQ EAAQAESKDG
210 220 230 240 250
KLTLPLAGIM QHDATKLLPS VTELFPEFRP GKVLRFLRLF GPGKNVPSVW
260 270 280 290 300
RSARRKRKKK HREPIQEEQI QEEECSVELE VNQKSLWNYD YAPPPPPEQC
310 320 330 340 350
LSDDEITMMA PVESKFSQST GDTDKVMDTK PRVAEWRYGP ARLWYDMLGV
360 370 380 390 400
PEDGSGFDYG FKMRKTEHEP AIKCKMMTKL RKLEESNGID LLADENFLMV
410 420 430 440 450
TQLHWEDDII WDGEDVKHKG TKPQRASLAG WLPSSMTRNA MAYNVQQGFA
460 470 480 490 500
ATLDDDKPWY SIFPIDNEDL VYGRWEDNII WDAQAMPRIL EPPVLTLDPN
510 520 530 540 550
DENLILEIPD EKEEATSNSP SKENKKESSL KKSRILLGKT GVIKEEPQQN
560 570 580 590 600
MSQPEVKDPW NLSNDEYYYP KQQGLRGTFG GNIIQHSIPA VELRQPFFPT
610 620 630 640 650
HMGPIKLRQF HRPPLKKYSF GALSQPGPHS VQPLLKHIKK KAKMREQERQ
660 670 680 690 700
ASGGGEMFFM RTPQDLTGKD GDLILAEYSE ENGPLMMQVG MATKIKNYYK
710 720 730 740 750
RKPGKDPGAP DCKYGETVYC HTSPFLGSLH PGQLLQAFEN NLFRAPIYLH
760 770 780 790 800
KMPETDFLII RTRQGYYIRE LVDIFVVGQQ CPLFEVPGPN SKRANTHIRD
810 820 830 840 850
FLQVFIYRLF WKSKDRPRRI RMEDIKKAFP SHSESSIRKR LKLCADFKRT
860 870 880 890 900
GMDSNWWVLK SDFRLPTEEE IRAMVSPEQC CAYYSMIAAE QRLKDAGYGE
910 920 930 940 950
KSFFAPEEEN EEDFQMKIDD EVRTAPWNTT RAFIAAMKGK CLLEVTGVAD
960 970 980 990 1000
PTGCGEGFSY VKIPNKPTQQ KDDKEPQPVK KTVTGTDADL RRLSLKNAKQ
1010 1020 1030 1040 1050
LLRKFGVPEE EIKKLSRWEV IDVVRTMSTE QARSGEGPMS KFARGSRFSV
1060 1070 1080 1090 1100
AEHQERYKEE CQRIFDLQNK VLSSTEVLST DTDSSSAEDS DFEEMGKNIE
1110 1120 1130 1140 1150
NMLQNKKTSS QLSREREEQE RKELQRMLLA AGSASAGNNH RDDDTASVTS
1160 1170 1180 1190 1200
LNSSATGRCL KIYRTFRDEE GKEYVRCETV RKPAVIDAYV RIRTTKDEEF
1210 1220 1230 1240 1250
IRKFALFDEQ HREEMRKERR RIQEQLRRLK RNQEKEKLKG PPEKKPKKMK
1260 1270 1280 1290 1300
ERPDLKLKCG ACGAIGHMRT NKFCPLYYQT NAPPSNPVAM TEEQEEELEK
1310 1320 1330 1340 1350
TVIHNDNEEL IKVEGTKIVL GKQLIESADE VRRKSLVLKF PKQQLPPKKK
1360 1370 1380 1390 1400
RRVGTTVHCD YLNRPHKSIH RRRTDPMVTL SSILESIIND MRDLPNTYPF
1410 1420 1430 1440 1450
HTPVNAKVVK DYYKIITRPM DLQTLRENVR KRLYPSREEF REHLELIVKN
1460 1470 1480 1490 1500
SATYNGPKHS LTQISQSMLD LCDEKLKEKE DKLARLEKAI NPLLDDDDQV
1510 1520 1530 1540 1550
AFSFILDNIV TQKMMAVPDS WPFHHPVNKK FVPDYYKVIV SPMDLETIRK
1560 1570 1580 1590 1600
NISKHKYQSR ESFLDDVNLI LANSVKYNGS ESQYTKTAQE IVNVCYQTLT
1610 1620 1630 1640 1650
EYDEHLTQLE KDICTAKEAA LEEAELESLD PMTPGPYTPQ PPDLYDNSTS
1660 1670 1680 1690 1700
LSVSRDASVY QDESNMSVLD IPSATSEKQL TQEGEDGDGD LADEEEGTVQ
1710 1720 1730 1740 1750
QPQASVLYED LLMSEGEDDE EDAGSDEEGD NPFSAIQLSE SGSDSDVGSG
1760 1770 1780 1790 1800
SIRPKQPRVL QENTRMGMEN EESMMSYEGD GGEVSRGLED SNISYGSYEE
1810 1820 1830 1840 1850
PDPKSNTQDT SFSSIGGYEV SEEEEDEEEQ RSGPSVLSQV HLSEDEEDSE
1860
DFHSIAGDSD MDSDE
Length:1,865
Mass (Da):211,868
Last modified:November 1, 1996 - v1
Checksum:iA81614946C0C0F24
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26114 mRNA. Translation: BAA05110.1.
PIRiI48155.
RefSeqiNP_001268498.1. NM_001281569.1.

Genome annotation databases

GeneIDi101838159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26114 mRNA. Translation: BAA05110.1 .
PIRi I48155.
RefSeqi NP_001268498.1. NM_001281569.1.

3D structure databases

ProteinModelPortali Q60544.
SMRi Q60544. Positions 1354-1620.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q60544.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101838159.

Organism-specific databases

CTDi 6872.

Phylogenomic databases

HOVERGENi HBG050223.

Family and domain databases

Gene3Di 1.10.1100.10. 1 hit.
1.20.920.10. 2 hits.
InterProi IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR011177. TAF1_animal.
IPR009067. TAF_II_230-bd.
IPR022591. TFIID_sub1_DUF3591.
[Graphical view ]
Pfami PF00439. Bromodomain. 2 hits.
PF12157. DUF3591. 1 hit.
PF09247. TBP-binding. 1 hit.
[Graphical view ]
PIRSFi PIRSF003047. TAF1_animal. 1 hit.
PRINTSi PR00503. BROMODOMAIN.
SMARTi SM00297. BROMO. 2 hits.
[Graphical view ]
SUPFAMi SSF47055. SSF47055. 1 hit.
SSF47370. SSF47370. 2 hits.
PROSITEi PS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster."
    Hayashida T., Sekiguchi T., Noguchi E., Sunamoto H., Ohba T., Nishimoto T.
    Gene 141:267-270(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTAF1_MESAU
AccessioniPrimary (citable) accession number: Q60544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3