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Q60544

- TAF1_MESAU

UniProt

Q60544 - TAF1_MESAU

Protein

Transcription initiation factor TFIID subunit 1

Gene

TAF1

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4 By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1. Binding to TAF1 or CIITA inhibits the histone acetyltransferase activity By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi1190 – 126879HMG boxBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. histone acetyltransferase activity Source: UniProtKB
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. DNA-templated transcription, initiation Source: InterPro
    2. histone acetylation Source: GOC
    3. mitotic G1 phase Source: BHF-UCL
    4. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    5. protein phosphorylation Source: GOC

    Keywords - Molecular functioni

    Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor TFIID subunit 1 (EC:2.3.1.48, EC:2.7.11.1)
    Alternative name(s):
    Cell cycle gene 1 protein
    TBP-associated factor 250 kDa
    Short name:
    p250
    Transcription initiation factor TFIID 250 kDa subunit
    Short name:
    TAF(II)250
    Short name:
    TAFII-250
    Short name:
    TAFII250
    Gene namesi
    Name:TAF1
    Synonyms:CCG1
    OrganismiMesocricetus auratus (Golden hamster)
    Taxonomic identifieri10036 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. MLL1 complex Source: UniProtKB
    2. transcription factor TFIID complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18651865Transcription initiation factor TFIID subunit 1PRO_0000278523Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei131 – 1311Phosphoserine; by autocatalysisBy similarity
    Modified residuei302 – 3021Phosphoserine; by autocatalysisBy similarity
    Modified residuei539 – 5391N6-acetyllysineBy similarity
    Disulfide bondi1359 ↔ 1614By similarity
    Modified residuei1821 – 18211PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Phosphoprotein

    Proteomic databases

    PRIDEiQ60544.

    Interactioni

    Subunit structurei

    TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H1.4, H2A, H2B, H3 and H4 (in vitro) By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ60544.
    SMRiQ60544. Positions 1354-1620.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 409409Protein kinase 1Add
    BLAST
    Domaini1392 – 146271Bromo 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1420 – 1865446Protein kinase 2Add
    BLAST
    Domaini1515 – 158571Bromo 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni512 – 971460Histone acetyltransferase (HAT)By similarityAdd
    BLAST
    Regioni1337 – 1624288Interaction with ASF1A and ASF1BBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1346 – 13538Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi152 – 1609Poly-Pro
    Compositional biasi178 – 1858Poly-Ser
    Compositional biasi293 – 2975Poly-Pro
    Compositional biasi1822 – 18298Poly-Glu

    Sequence similaritiesi

    Belongs to the TAF1 family.Curated
    Contains 2 bromo domains.PROSITE-ProRule annotation
    Contains 1 HMG box DNA-binding domain.Curated
    Contains 2 protein kinase domains.Curated

    Keywords - Domaini

    Bromodomain, Repeat

    Phylogenomic databases

    HOVERGENiHBG050223.

    Family and domain databases

    Gene3Di1.10.1100.10. 1 hit.
    1.20.920.10. 2 hits.
    InterProiIPR001487. Bromodomain.
    IPR018359. Bromodomain_CS.
    IPR011177. TAF1_animal.
    IPR009067. TAF_II_230-bd.
    IPR022591. TFIID_sub1_DUF3591.
    [Graphical view]
    PfamiPF00439. Bromodomain. 2 hits.
    PF12157. DUF3591. 1 hit.
    PF09247. TBP-binding. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003047. TAF1_animal. 1 hit.
    PRINTSiPR00503. BROMODOMAIN.
    SMARTiSM00297. BROMO. 2 hits.
    [Graphical view]
    SUPFAMiSSF47055. SSF47055. 1 hit.
    SSF47370. SSF47370. 2 hits.
    PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
    PS50014. BROMODOMAIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60544-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLPATGASR SAAIMSDTDS DEDSSGGGPF SLTGFLFGNI NGAGQLEGES     50
    VLDDECKKHL AGLGALGLGS LITELTANEE LAGTDGALVN DEGWIRSRED 100
    AVDYSDINEV AEDESRRYQQ TMGSLQPLCH SADYDEDDYD ADCEDIDCKL 150
    MPPPPPPPGP VKKEKDQDGL TGEKVDFSSS SDSESEMGPQ EAAQAESKDG 200
    KLTLPLAGIM QHDATKLLPS VTELFPEFRP GKVLRFLRLF GPGKNVPSVW 250
    RSARRKRKKK HREPIQEEQI QEEECSVELE VNQKSLWNYD YAPPPPPEQC 300
    LSDDEITMMA PVESKFSQST GDTDKVMDTK PRVAEWRYGP ARLWYDMLGV 350
    PEDGSGFDYG FKMRKTEHEP AIKCKMMTKL RKLEESNGID LLADENFLMV 400
    TQLHWEDDII WDGEDVKHKG TKPQRASLAG WLPSSMTRNA MAYNVQQGFA 450
    ATLDDDKPWY SIFPIDNEDL VYGRWEDNII WDAQAMPRIL EPPVLTLDPN 500
    DENLILEIPD EKEEATSNSP SKENKKESSL KKSRILLGKT GVIKEEPQQN 550
    MSQPEVKDPW NLSNDEYYYP KQQGLRGTFG GNIIQHSIPA VELRQPFFPT 600
    HMGPIKLRQF HRPPLKKYSF GALSQPGPHS VQPLLKHIKK KAKMREQERQ 650
    ASGGGEMFFM RTPQDLTGKD GDLILAEYSE ENGPLMMQVG MATKIKNYYK 700
    RKPGKDPGAP DCKYGETVYC HTSPFLGSLH PGQLLQAFEN NLFRAPIYLH 750
    KMPETDFLII RTRQGYYIRE LVDIFVVGQQ CPLFEVPGPN SKRANTHIRD 800
    FLQVFIYRLF WKSKDRPRRI RMEDIKKAFP SHSESSIRKR LKLCADFKRT 850
    GMDSNWWVLK SDFRLPTEEE IRAMVSPEQC CAYYSMIAAE QRLKDAGYGE 900
    KSFFAPEEEN EEDFQMKIDD EVRTAPWNTT RAFIAAMKGK CLLEVTGVAD 950
    PTGCGEGFSY VKIPNKPTQQ KDDKEPQPVK KTVTGTDADL RRLSLKNAKQ 1000
    LLRKFGVPEE EIKKLSRWEV IDVVRTMSTE QARSGEGPMS KFARGSRFSV 1050
    AEHQERYKEE CQRIFDLQNK VLSSTEVLST DTDSSSAEDS DFEEMGKNIE 1100
    NMLQNKKTSS QLSREREEQE RKELQRMLLA AGSASAGNNH RDDDTASVTS 1150
    LNSSATGRCL KIYRTFRDEE GKEYVRCETV RKPAVIDAYV RIRTTKDEEF 1200
    IRKFALFDEQ HREEMRKERR RIQEQLRRLK RNQEKEKLKG PPEKKPKKMK 1250
    ERPDLKLKCG ACGAIGHMRT NKFCPLYYQT NAPPSNPVAM TEEQEEELEK 1300
    TVIHNDNEEL IKVEGTKIVL GKQLIESADE VRRKSLVLKF PKQQLPPKKK 1350
    RRVGTTVHCD YLNRPHKSIH RRRTDPMVTL SSILESIIND MRDLPNTYPF 1400
    HTPVNAKVVK DYYKIITRPM DLQTLRENVR KRLYPSREEF REHLELIVKN 1450
    SATYNGPKHS LTQISQSMLD LCDEKLKEKE DKLARLEKAI NPLLDDDDQV 1500
    AFSFILDNIV TQKMMAVPDS WPFHHPVNKK FVPDYYKVIV SPMDLETIRK 1550
    NISKHKYQSR ESFLDDVNLI LANSVKYNGS ESQYTKTAQE IVNVCYQTLT 1600
    EYDEHLTQLE KDICTAKEAA LEEAELESLD PMTPGPYTPQ PPDLYDNSTS 1650
    LSVSRDASVY QDESNMSVLD IPSATSEKQL TQEGEDGDGD LADEEEGTVQ 1700
    QPQASVLYED LLMSEGEDDE EDAGSDEEGD NPFSAIQLSE SGSDSDVGSG 1750
    SIRPKQPRVL QENTRMGMEN EESMMSYEGD GGEVSRGLED SNISYGSYEE 1800
    PDPKSNTQDT SFSSIGGYEV SEEEEDEEEQ RSGPSVLSQV HLSEDEEDSE 1850
    DFHSIAGDSD MDSDE 1865
    Length:1,865
    Mass (Da):211,868
    Last modified:November 1, 1996 - v1
    Checksum:iA81614946C0C0F24
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26114 mRNA. Translation: BAA05110.1.
    PIRiI48155.
    RefSeqiNP_001268498.1. NM_001281569.1.

    Genome annotation databases

    GeneIDi101838159.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26114 mRNA. Translation: BAA05110.1 .
    PIRi I48155.
    RefSeqi NP_001268498.1. NM_001281569.1.

    3D structure databases

    ProteinModelPortali Q60544.
    SMRi Q60544. Positions 1354-1620.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q60544.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101838159.

    Organism-specific databases

    CTDi 6872.

    Phylogenomic databases

    HOVERGENi HBG050223.

    Family and domain databases

    Gene3Di 1.10.1100.10. 1 hit.
    1.20.920.10. 2 hits.
    InterProi IPR001487. Bromodomain.
    IPR018359. Bromodomain_CS.
    IPR011177. TAF1_animal.
    IPR009067. TAF_II_230-bd.
    IPR022591. TFIID_sub1_DUF3591.
    [Graphical view ]
    Pfami PF00439. Bromodomain. 2 hits.
    PF12157. DUF3591. 1 hit.
    PF09247. TBP-binding. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003047. TAF1_animal. 1 hit.
    PRINTSi PR00503. BROMODOMAIN.
    SMARTi SM00297. BROMO. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47055. SSF47055. 1 hit.
    SSF47370. SSF47370. 2 hits.
    PROSITEi PS00633. BROMODOMAIN_1. 2 hits.
    PS50014. BROMODOMAIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster."
      Hayashida T., Sekiguchi T., Noguchi E., Sunamoto H., Ohba T., Nishimoto T.
      Gene 141:267-270(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiTAF1_MESAU
    AccessioniPrimary (citable) accession number: Q60544
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3