Q60544 (TAF1_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 1
EC=2.7.11.1

Alternative name(s):
Cell cycle gene 1 protein
TBP-associated factor 250 kDa
Short name=p250
Transcription initiation factor TFIID 250 kDa subunit
Short name=TAF(II)250
Short name=TAFII-250
Short name=TAFII250

Gene names

Name:	TAF1
Synonyms:	CCG1

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	1865 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1 By similarity.
Subunit structure	TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H1.4, H2A, H2B, H3 and H4 (in vitro) By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the TAF1 family. Contains 2 bromo domains. Contains 1 HMG box DNA-binding domain. Contains 2 protein kinase domains.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Domain	Bromodomain Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Disulfide bond Phosphoprotein
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: InterPro G1 phase of mitotic cell cycle Inferred from mutant phenotype Ref.1. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 9660973. Source: BHF-UCL
Cellular_component	MLL1 complex Inferred from sequence or structural similarity. Source: UniProtKB transcription factor TFIID complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW histone acetyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1865	1865	Transcription initiation factor TFIID subunit 1		PRO_0000278523
Regions
	Domain	1 – 409	409	Protein kinase 1
	Domain	1392 – 1462	71	Bromo 1
	Domain	1420 – 1865	446	Protein kinase 2
	Domain	1515 – 1585	71	Bromo 2
	DNA binding	1190 – 1268	79	HMG box By similarity
	Region	1337 – 1624	288	Interaction with ASF1A and ASF1B By similarity
	Motif	1346 – 1353	8	Nuclear localization signal Potential
	Compositional bias	152 – 160	9	Poly-Pro
	Compositional bias	178 – 185	8	Poly-Ser
	Compositional bias	293 – 297	5	Poly-Pro
	Compositional bias	1822 – 1829	8	Poly-Glu
Amino acid modifications
	Modified residue	131	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	302	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	1821	1	Phosphoserine By similarity
	Disulfide bond	1359 ↔ 1614		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60544 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A81614946C0C0F24	FASTA	1,865	211,868
	10 20 30 40 50 60 MLLPATGASR SAAIMSDTDS DEDSSGGGPF SLTGFLFGNI NGAGQLEGES VLDDECKKHL 70 80 90 100 110 120 AGLGALGLGS LITELTANEE LAGTDGALVN DEGWIRSRED AVDYSDINEV AEDESRRYQQ 130 140 150 160 170 180 TMGSLQPLCH SADYDEDDYD ADCEDIDCKL MPPPPPPPGP VKKEKDQDGL TGEKVDFSSS 190 200 210 220 230 240 SDSESEMGPQ EAAQAESKDG KLTLPLAGIM QHDATKLLPS VTELFPEFRP GKVLRFLRLF 250 260 270 280 290 300 GPGKNVPSVW RSARRKRKKK HREPIQEEQI QEEECSVELE VNQKSLWNYD YAPPPPPEQC 310 320 330 340 350 360 LSDDEITMMA PVESKFSQST GDTDKVMDTK PRVAEWRYGP ARLWYDMLGV PEDGSGFDYG 370 380 390 400 410 420 FKMRKTEHEP AIKCKMMTKL RKLEESNGID LLADENFLMV TQLHWEDDII WDGEDVKHKG 430 440 450 460 470 480 TKPQRASLAG WLPSSMTRNA MAYNVQQGFA ATLDDDKPWY SIFPIDNEDL VYGRWEDNII 490 500 510 520 530 540 WDAQAMPRIL EPPVLTLDPN DENLILEIPD EKEEATSNSP SKENKKESSL KKSRILLGKT 550 560 570 580 590 600 GVIKEEPQQN MSQPEVKDPW NLSNDEYYYP KQQGLRGTFG GNIIQHSIPA VELRQPFFPT 610 620 630 640 650 660 HMGPIKLRQF HRPPLKKYSF GALSQPGPHS VQPLLKHIKK KAKMREQERQ ASGGGEMFFM 670 680 690 700 710 720 RTPQDLTGKD GDLILAEYSE ENGPLMMQVG MATKIKNYYK RKPGKDPGAP DCKYGETVYC 730 740 750 760 770 780 HTSPFLGSLH PGQLLQAFEN NLFRAPIYLH KMPETDFLII RTRQGYYIRE LVDIFVVGQQ 790 800 810 820 830 840 CPLFEVPGPN SKRANTHIRD FLQVFIYRLF WKSKDRPRRI RMEDIKKAFP SHSESSIRKR 850 860 870 880 890 900 LKLCADFKRT GMDSNWWVLK SDFRLPTEEE IRAMVSPEQC CAYYSMIAAE QRLKDAGYGE 910 920 930 940 950 960 KSFFAPEEEN EEDFQMKIDD EVRTAPWNTT RAFIAAMKGK CLLEVTGVAD PTGCGEGFSY 970 980 990 1000 1010 1020 VKIPNKPTQQ KDDKEPQPVK KTVTGTDADL RRLSLKNAKQ LLRKFGVPEE EIKKLSRWEV 1030 1040 1050 1060 1070 1080 IDVVRTMSTE QARSGEGPMS KFARGSRFSV AEHQERYKEE CQRIFDLQNK VLSSTEVLST 1090 1100 1110 1120 1130 1140 DTDSSSAEDS DFEEMGKNIE NMLQNKKTSS QLSREREEQE RKELQRMLLA AGSASAGNNH 1150 1160 1170 1180 1190 1200 RDDDTASVTS LNSSATGRCL KIYRTFRDEE GKEYVRCETV RKPAVIDAYV RIRTTKDEEF 1210 1220 1230 1240 1250 1260 IRKFALFDEQ HREEMRKERR RIQEQLRRLK RNQEKEKLKG PPEKKPKKMK ERPDLKLKCG 1270 1280 1290 1300 1310 1320 ACGAIGHMRT NKFCPLYYQT NAPPSNPVAM TEEQEEELEK TVIHNDNEEL IKVEGTKIVL 1330 1340 1350 1360 1370 1380 GKQLIESADE VRRKSLVLKF PKQQLPPKKK RRVGTTVHCD YLNRPHKSIH RRRTDPMVTL 1390 1400 1410 1420 1430 1440 SSILESIIND MRDLPNTYPF HTPVNAKVVK DYYKIITRPM DLQTLRENVR KRLYPSREEF 1450 1460 1470 1480 1490 1500 REHLELIVKN SATYNGPKHS LTQISQSMLD LCDEKLKEKE DKLARLEKAI NPLLDDDDQV 1510 1520 1530 1540 1550 1560 AFSFILDNIV TQKMMAVPDS WPFHHPVNKK FVPDYYKVIV SPMDLETIRK NISKHKYQSR 1570 1580 1590 1600 1610 1620 ESFLDDVNLI LANSVKYNGS ESQYTKTAQE IVNVCYQTLT EYDEHLTQLE KDICTAKEAA 1630 1640 1650 1660 1670 1680 LEEAELESLD PMTPGPYTPQ PPDLYDNSTS LSVSRDASVY QDESNMSVLD IPSATSEKQL 1690 1700 1710 1720 1730 1740 TQEGEDGDGD LADEEEGTVQ QPQASVLYED LLMSEGEDDE EDAGSDEEGD NPFSAIQLSE 1750 1760 1770 1780 1790 1800 SGSDSDVGSG SIRPKQPRVL QENTRMGMEN EESMMSYEGD GGEVSRGLED SNISYGSYEE 1810 1820 1830 1840 1850 1860 PDPKSNTQDT SFSSIGGYEV SEEEEDEEEQ RSGPSVLSQV HLSEDEEDSE DFHSIAGDSD MDSDE

References

[1]	"The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster." Hayashida T., Sekiguchi T., Noguchi E., Sunamoto H., Ohba T., Nishimoto T. Gene 141:267-270(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26114 mRNA. Translation: BAA05110.1.
PIR	I48155.
3D structure databases
HSSP	HSSP built from PDB template 1EQF based on UniProtKB P21675.
ProteinModelPortal	Q60544.
SMR	Q60544. Positions 1354-1620.
ModBase	Search...
Proteomic databases
PRIDE	Q60544.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050223.
Family and domain databases
Gene3D	1.10.1100.10. 1 hit. 1.20.920.10. 2 hits.
InterPro	IPR001487. Bromodomain. IPR018359. Bromodomain_CS. IPR011177. TAF1_animal. IPR009067. TAF_II_230-bd. IPR022591. TFIID_sub1_DUF3591. [Graphical view]
Pfam	PF00439. Bromodomain. 2 hits. PF12157. DUF3591. 1 hit. PF09247. TBP-binding. 1 hit. [Graphical view]
PIRSF	PIRSF003047. TAF1_animal. 1 hit.
PRINTS	PR00503. BROMODOMAIN.
SMART	SM00297. BROMO. 2 hits. [Graphical view]
SUPFAM	SSF47370. Bromodomain. 2 hits. SSF47055. TAF_II_230. 1 hit.
PROSITE	PS00633. BROMODOMAIN_1. 2 hits. PS50014. BROMODOMAIN_2. 2 hits. PS50118. HMG_BOX_2. False negative. PS50011. PROTEIN_KINASE_DOM. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TAF1_MESAU

Accession

Primary (citable) accession number: Q60544

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program