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Protein

Zinc finger E-box-binding homeobox 1

Gene

ZEB1

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a transcriptional repressor. Binds to E-box sequences in the immunoglobulin heavy chain enhancer as well as in the regulatory regions of many other tissue-specific genes. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri94 – 11724C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri124 – 14623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri164 – 18623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri192 – 21625C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
DNA bindingi504 – 56360Homeobox; atypicalAdd
BLAST
Zinc fingeri827 – 84923C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri855 – 87723C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri883 – 90422C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger E-box-binding homeobox 1
Alternative name(s):
Transcription factor 8
Short name:
TCF-8
Zinc finger protein BZP
Gene namesi
Name:ZEB1
Synonyms:BZP, TCF8
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10431043Zinc finger E-box-binding homeobox 1PRO_0000047232Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei237 – 2371PhosphoserineBy similarity
Modified residuei246 – 2461PhosphoserineBy similarity
Cross-linki271 – 271Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki417 – 417Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki428 – 428Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki439 – 439Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei602 – 6021PhosphoserineBy similarity
Modified residuei609 – 6091PhosphoserineBy similarity
Modified residuei616 – 6161PhosphoserineBy similarity
Modified residuei623 – 6231PhosphoserineBy similarity
Modified residuei625 – 6251PhosphothreonineBy similarity
Cross-linki697 – 697Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Interacts (via N-terminus) with SMARCA4/BRG1.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ60542.
SMRiQ60542. Positions 506-564.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi912 – 1043132Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri94 – 11724C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri124 – 14623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri164 – 18623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri192 – 21625C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri827 – 84923C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri855 – 87723C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri883 – 90422C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG004697.
OrthoDBiEOG091G0F7Y.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProiIPR008598. Di19_Zn_binding_dom.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60542-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEDELPADQ TVLPGGSDRE GGAKNCWQDD VKDDECDSDA ENEQNHDPNV
60 70 80 90 100
EEFLQQQDTA IIYPEAPEED QRQGTPEASG QDENGAADAF SQLLTCPYCD
110 120 130 140 150
RGYKRFTSLK EHIKYRHEKN EDNFSCSLCS YTFAYRTQLE RHMTSHKSGR
160 170 180 190 200
EQRHVTQSGG NRKFKCTECG KAFKYKHHLK EHLRIHSGEK PYECPNCKKR
210 220 230 240 250
FSHSGSYSSH ISSKKCISLM PVNGRPRSGL KTSQCPSPSL SASPGSPTRP
260 270 280 290 300
QIRQKIENKP LQEPLSVNQI KTEPVDYEFK PIVVASGINC STPLQNGVFS
310 320 330 340 350
GGGQLQATSS PQGVVQAVVL PTVGLVSPIS INLSDIQNVL KVALDGNVIR
360 370 380 390 400
QVLENNQASL ASKEQEAVNA SSIQQGGHSV ISAISLPLVD QDGTTKIIIN
410 420 430 440 450
YSLEQPSQLQ VVPQNLKIEN PAPTNSCKSE KLPEDLTVKS EKDKGFDGAV
460 470 480 490 500
DGSTCLLYDD CPGDLNALPE LKKHYDPEHP AQPPPPAPEA EKPEASASSA
510 520 530 540 550
RDGDLSPSQP PLKNLLSLLK AYYALNAQPN SEELSKIADS VNLPLDVVKK
560 570 580 590 600
WFEKMQAGQI PGQSPEPPSP ETGTVNIPAK SDEQPQPADG SEPQEDSASG
610 620 630 640 650
QSPLKMTSSP VLPVGSAING SRSCTSPPSP LNLSSARSLQ GYFCVADGAQ
660 670 680 690 700
EEPQVEPLDL SLPKQQGELL ERSTISSVYQ NSVYSVQEEP LNLSCVKKEP
710 720 730 740 750
QEDSCVTDSE PVVNVIPPSA NPINIAIPTV TAQLPTIVAI ADQNSVPCLR
760 770 780 790 800
ALAANKQTIL IPQVAYTYAT TVSPAMQEPP VKVIQPNGNQ DERQDTSSEG
810 820 830 840 850
VSTVEDQNDS DSTPPKKKTR KTENGMYACD LCDKIFQKSS SLLRHKYEHT
860 870 880 890 900
GKRPHECGIC KKAFKHKHHL IEHMRLHSGE KPYQCDKCGK RFSHSGSYSQ
910 920 930 940 950
HMNHRYSYCK REAEERDGTE QEQEEAGLEA LMNEHVGARA SPSQADSDER
960 970 980 990 1000
ESLTREEDED SEKEEEEEED KEMEELQEEK ECGNAQAEEE EEEEEEEMDG
1010 1020 1030 1040
AKDEAAAKTD GAVENGAAPQ AGSLEQKASE SSEQLSEETT NEA
Length:1,043
Mass (Da):114,203
Last modified:November 1, 1996 - v1
Checksum:i5F8BE23DD1BCC667
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13856 mRNA. Translation: AAC37667.1.
PIRiA56037.
RefSeqiNP_001268549.1. NM_001281620.1.

Genome annotation databases

GeneIDi101832581.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13856 mRNA. Translation: AAC37667.1.
PIRiA56037.
RefSeqiNP_001268549.1. NM_001281620.1.

3D structure databases

ProteinModelPortaliQ60542.
SMRiQ60542. Positions 506-564.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101832581.

Organism-specific databases

CTDi6935.

Phylogenomic databases

HOVERGENiHBG004697.
OrthoDBiEOG091G0F7Y.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProiIPR008598. Di19_Zn_binding_dom.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZEB1_MESAU
AccessioniPrimary (citable) accession number: Q60542
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.